LOCUS       NZ_CP072952             2328 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae MGH 39 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP072952
VERSION     NZ_CP072952.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138579
            Assembly: GCF_018075085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae MGH 39
  ORGANISM  Klebsiella pneumoniae MGH 39
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH39
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 185x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 09:02:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,520
            Genes (coding)                    :: 5,378
            CDSs (with protein)               :: 5,378
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 67 of 142
            Pseudo Genes (incomplete)         :: 84 of 142
            Pseudo Genes (internal stop)      :: 23 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072952.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Klebsiella pneumoniae MGH 39"
                     /mol_type="genomic DNA"
                     /strain="MGH 39"
                     /isolation_source="urine"
                     /db_xref="taxon:1328373"
                     /plasmid="unnamed3"
     gene            join(2029..2328,1..885)
                     /locus_tag="IBD60_RS28000"
     CDS             join(2029..2328,1..885)
                     /locus_tag="IBD60_RS28000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_032432587.1"
                     /translation="MDLTIEQRRIANAKIMIAQELATNSGTFIEIQIDRMIKEIISKVK
                     ISASDASKEQYRKLYKSCISKAEGDLKSCLMSCKTRASFDKTRTSFRFSIMEEIQKLRR
                     ESEKAMKSKDYDLGKKKIQEAFKLAVKFEDEFLSENRIIFNDVKNKKGFKRVSFSKKKG
                     LKKAPSADEIIKSFSVNNNISDRYALAFSVIATFGIRPAELQKGVKLQFHDGMIYAVVQ
                     GAKVGSDKGQKVRAMGSALRENNEGDKILMDAIYKAGGEVVVTQNKKDYESLRKYLNRH
                     HPGTSLYTYRHKVASDLKASGLQKRQIAEFLGHRTTDSQQYYGYSRSGSGGRSMTAKGS
                     NEVRVKPDIKDYLKPATKPTAGSGGGALGSKIKQAKTSISTTSKVPKFKPPGIK"
                     /name="site-specific integrase"
ORIGIN
        1 aagttaaggc gtgaatctga aaaggcgatg aagtctaaag attacgatct tggaaagaaa
       61 aaaattcaag aagcatttaa actagcggtt aaatttgaag atgaattttt atctgaaaat
      121 agaattattt ttaatgatgt caaaaataaa aaaggtttta aaagggtgtc attcagtaag
      181 aaaaaaggac tgaaaaaagc accttccgca gatgaaataa taaagtcatt tagtgttaat
      241 aataatataa gtgatcgcta tgctctggcg ttctctgtga tcgctacttt tggaatacgt
      301 ccggcagaac tccaaaaagg ggttaaactc cagtttcatg atggaatgat ttatgcagtt
      361 gtacaaggtg ctaaagttgg atcggataaa ggccagaaag taagagctat ggggtctgca
      421 ttacgtgaaa ataatgaggg tgataaaatt ctaatggatg ccatctataa ggcaggtggt
      481 gaagttgtag ttacgcaaaa taaaaaagac tatgagagtt taagaaagta tttaaataga
      541 catcatccgg gaacatcgct ttatacatac cgccataaag tcgccagtga cctgaaggcg
      601 tcaggtctcc agaagcgcca gatagccgag tttctggggc atagaaccac ggactcccag
      661 cagtattacg gttattcaag atccggcagt ggtggccgtt ctatgacggc taaggggtct
      721 aatgaagtga gggttaaacc agatataaaa gactacttaa aaccagcaac aaagcctact
      781 gctggaagcg gtggaggtgc tttaggtagc aagataaaac aagctaaaac ctcgatttct
      841 accacgtcga aagtgccgaa gtttaagcct ccgggcatca aataagatgc atcaatattt
      901 ttgatagact attgacgtat aacgcaaatc ggttataaca ggatccagat acaacaaaac
      961 cagcttgcgc tggttttgag ggactaaaaa agtttggcga ctttttaagt cttgtaatca
     1021 agtgagttca gcactggatt acgaatgtag atcaaatcta acctacatct tcgtaaaagt
     1081 caaacctacc tgcattacat ttcttaaaag tttcctagca ttttcaaacc cttaagctga
     1141 tacggaaagc aaaaccgatt gtgtccggta cttggtgtgg agccgcgtat ggagtcatat
     1201 gcatgtgatt gttcgccaag tggtatcgag taggactaaa cggggagtac tgcgaacgtc
     1261 caaccgggaa cgagtcacta agtcgtatga tgagcatcag gccagctatg agaaagccca
     1321 gccgataata tggggttaga tgccgtaagt tacgatatgt ggcgactgag ggggtgcagt
     1381 agggtgccag catagccgaa aggtcatgca gctctaagtc tcagctcttt gggtgaagct
     1441 aaacgacgct ttaccaaagg atcgtcaacc tgatcagcta aggctatcgg ggaggcgaaa
     1501 ctttgggtga agcgcccttt ttcccggcgc ggaacatcct tttagaattc cggcgtagca
     1561 caggccgaag gcacgatcag agttagtaca gctataaggt aaaagaaaaa ccgccgacaa
     1621 gaaaaagtac gcaacgctcg ccgcaagaca aaatatttta ttttgtcgag gaagcgtaat
     1681 atccactaaa cgaatataga gcacttacgc gccggactaa caaccggaaa aaacattttt
     1741 tggcgatgcc actttttgct ccgcactctc tctatttgtg gatagataga cagaataaaa
     1801 ataaaagtta aaatatatat ataacattat tgggacagtg tttttatgtc ccaataatgt
     1861 gggcgtgtct tacattttcg cttttgaaaa tggaagtttg cggtagcaaa aacgccttgc
     1921 ccttcgacct ttgcgattgg gaagattgcg ccagctttct gacctttcgc attgggagat
     1981 gggcatataa agacgatgga ggaataaaat ataaagcagg ggggtaatat ggatttaaca
     2041 atagaacaaa gaaggatcgc caacgcaaaa ataatgattg cacaagagtt ggcaaccaat
     2101 agcggtactt ttatcgaaat tcaaattgac cgaatgataa aagagataat aagcaaagtt
     2161 aagatctccg ctagcgatgc atccaaagag caatacagaa aactatacaa atcatgcata
     2221 tcgaaggcag aaggagatct gaaaagctgc ttgatgagct gtaaaactcg tgcaagcttt
     2281 gataaaacaa gaacgtcatt caggttctct attatggaag aaatacag
//
