LOCUS       NZ_CP072948             2511 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 7 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP072948
VERSION     NZ_CP072948.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138547
            Assembly: GCF_018075005.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 7
  ORGANISM  Enterobacter sp. MGH 7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 162x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,185
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 31 of 91
            Pseudo Genes (incomplete)         :: 56 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 18 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072948.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter sp. MGH 7"
                     /mol_type="genomic DNA"
                     /strain="MGH 7"
                     /isolation_source="wound"
                     /db_xref="taxon:1329816"
                     /plasmid="unnamed3"
     gene            complement(174..359)
                     /locus_tag="IAU58_RS25810"
     CDS             complement(174..359)
                     /locus_tag="IAU58_RS25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(726..953)
                     /locus_tag="IAU58_RS25815"
     CDS             complement(726..953)
                     /locus_tag="IAU58_RS25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1381..1524)
                     /locus_tag="IAU58_RS26030"
     CDS             complement(1381..1524)
                     /locus_tag="IAU58_RS26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     Typing          48..127
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(1360..1920)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gggcagctcc ccggtttttt tttgcctgtt cccggggaac cactcaggac aggatgtgta
       61 tttgtaatac cgcgcacgcg ccggcatcac aaatacccag gtcagggctg cgccccgaca
      121 ccccgccatc gcctcacggc cgcgcactga attgcgcacc cgttcaccga ttttcacgca
      181 tcttcctcgc ctgtctgtgc gttcagcatg gtgtaaagtg cttcggcctg atcgtgcagg
      241 tgctcacagc agccggcagc ctcatccaga tccatctgtt cgagccgctc gaggaggagg
      301 agtgtctgtg agcggataaa tttggccatg ttgagggcgg cggattcctg tctggtcact
      361 ggtgattttc cccgtctggt gatgattttt gcgtgaaaag agcatgccgt cattctggtg
      421 catagtcatg catgccgtta aaatttaaca ggagcgtttc tggcgggttc cggggtggtt
      481 tgttgtggtt tttggtcatg gttccgtcag aaaagcgctg agtgcgtctg aggcggtgcg
      541 ggcaacggat gttatggtaa attcttgggg ggcttgatta taaaaaccgc acttcctcta
      601 atgtcttgtg ctttttagac aaccctggaa gcttttagct ttggtggtgc aatttgctgg
      661 gattttggcg gagaacccca ggtaaagaaa aaggccacat tagcggcctt tttcggagaa
      721 gatgctcagt gcggaatttc ggagacttct ttaacgtcgg ttttattgat ctgctgtttg
      781 acaccattcg cgtctgtata gccaagcagg cctgagtctg attctttagg tttgccgtcg
      841 ctgacaatag tgcgcccgtc attggtgtgt atggcatagc ttgtgcgcgt gcagccggtg
      901 agggctgaaa gcgcaactgc cgctgcgaac acggaaacaa aaatcttttt caaagccagc
      961 tccttttatc catatcagtt ttggtcacta accatctaac tataggtcat tttttatgtg
     1021 aaaagaggct tgatggtggg ctgcatatcg agttaggtgc gaggtgttat ggttaaagtg
     1081 tggtgtctgg cggggagggg gcagtgcggg cttatatctg atgtaatccg accgatacat
     1141 ataccaagat gatttaagta ttcctctttg tgaggttgga tgtctaattt ataaaaggat
     1201 cttcttgaga tccttttttt tcgcgcgtaa tctcttgccc tgtaaacgaa aaaaccaccc
     1261 ggcaaggtgg tttttcgaag gttcagcaaa ccggcaagtt tctgaaccgt ggtaacaggg
     1321 tgtacaagac cgctgccacc aaatttgtcc ttctagtgta gccgcagtca ggcgtcaact
     1381 tcaagaactc tgcaccagta atcttgtata ccccttacca gtggccgctg ccagtggcgt
     1441 ttttacgtgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcagtc
     1501 ggactgaacg gggggttcgt gcatacagtc catcctggag cgaactgcct tcccggaacc
     1561 gagtgtcagg cgtggaatga gaaaccgcgg ccataacagc ggagtgacac cggtaaaccg
     1621 aaaggcagga atgcggggga gcacgaggga gccatcaggg ggaaacgcct ggtatcttta
     1681 agccgcatcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg ctcgtcaggg
     1741 gggcggagcc tatggaaaaa cggcttcggc gcggccttat gccttcttcg ctaagtatct
     1801 tcctggcatc ccctagctgg catcccctag gaaatttctg ctccacccgt aagcccgtac
     1861 cgctcgccgc agccgaacga ccgagcgcag cgagtgagtg agcgaggaag cggaatatat
     1921 cctgtattac attttctcct gacgcgtttt ctttcatttt ctgcgcctgt cttatgtagc
     1981 agttatgcta tgcgttactg cggtgttaca ttttagccag tatacactcc gctagcgctg
     2041 agtgactggt ccggcgctgc gcccggaacc cgcctgtacc ggttcagcag ccgttccggg
     2101 ctgactgcaa tttttttatt ttcctgcccg ctaccctgta aacctttctt ctgcgttgcc
     2161 gttaacagga ggtaagccat gtttggtttt tcttccggag acctgatgac tcttgatgat
     2221 gagcgacacc tgtttgtgtc tgttgtttcg ggctggctcc ggcgtgccgg ggggatgccg
     2281 gagccatgat gctggatgca taccggcaga ttttgtctga aactgaaccg gccgtaagga
     2341 cggtcatgct cgaatttctt gaatccgttc gcattcatta catttcgtcc tgagcatttt
     2401 ctgtatttct gtccatactt aaacgaggcg gccgtcacgg acgatggtcg tcgttgcatg
     2461 atgctgttat ggatgagtga aagtaatcta atctaacttg acttaccttc c
//
