LOCUS       NZ_CP072947             5411 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter sp. MGH 7 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP072947
VERSION     NZ_CP072947.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02138547
            Assembly: GCF_018075005.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. MGH 7
  ORGANISM  Enterobacter sp. MGH 7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Earl,A.M., Manson,A.L., Bhattacharyya,R., Brennan-Krohn,T.,
            Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M., Gussin,G.,
            Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A., Peterson,E.,
            Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Chapman,S., Howe,W.,
            Walker,B. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 162x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 03:14:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,185
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 31 of 91
            Pseudo Genes (incomplete)         :: 56 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 18 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072947.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Enterobacter sp. MGH 7"
                     /mol_type="genomic DNA"
                     /strain="MGH 7"
                     /isolation_source="wound"
                     /db_xref="taxon:1329816"
                     /plasmid="unnamed2"
     gene            1002..1325
                     /locus_tag="IAU58_RS25775"
     CDS             1002..1325
                     /locus_tag="IAU58_RS25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1534..>1968
                     /locus_tag="IAU58_RS26025"
                     /pseudo=""
     CDS             1534..>1968
                     /locus_tag="IAU58_RS26025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2003..2494
                     /locus_tag="IAU58_RS25785"
     CDS             2003..2494
                     /locus_tag="IAU58_RS25785"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2501..2716
                     /locus_tag="IAU58_RS25790"
     CDS             2501..2716
                     /locus_tag="IAU58_RS25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2913..3077
                     /locus_tag="IAU58_RS25795"
     CDS             2913..3077
                     /locus_tag="IAU58_RS25795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164088320.1"
                     /translation="MKLLNVIEALLNCLYLAAELWDWLEKHLPVFKALALILVWLYTPC
                     IWLHGYAVG"
                     /name="hypothetical protein"
     gene            complement(4249..4542)
                     /locus_tag="IAU58_RS25800"
     CDS             complement(4249..4542)
                     /locus_tag="IAU58_RS25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609279.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLEVSQSALSRLLN
                     GKTDLSYEMALRLSKVIGRSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            4880..5161
                     /locus_tag="IAU58_RS25805"
     CDS             4880..5161
                     /locus_tag="IAU58_RS25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000594459.1"
                     /translation="MIKNIKHKGLRSFAENGNTAGIQPHLAKKLRMILTTLSTATCLQD
                     IVDVRALGCHPLKGERKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          716..889
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(205..792)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.709"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1316..2808
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaagcgatt gaggctttca gggttcttcc ttgactgatt aaatcgacaa agcgtatctt
       61 atgttcgaaa cgaaaaaacc gcctggggag gcggtttttt cgagggttca gtaagttggg
      121 gaacttctga accgtggtaa cagggtgtac aagaccgctg aaaccaaatt ctgtccttcc
      181 agtttagcct cagtatggct ctgacttcaa gaactctgca tcagtaatct cttgtacacc
      241 ctcttaccaa tggccgcagc cagtggcgtt ttgttgtgtc tttccgggtt ggactcaagt
      301 cgatagttac cggataagac gctgcggtcg gactgaacgg ggggttcgtg catacagtcc
      361 agcttggagc gaactgcctt cccggaactg agtgtcaggc gtggaatgag ataaacgcgg
      421 ccataacagc ggagtgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
      481 gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
      541 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc
      601 cgtggccttc tcgcttccct gttaagcttc ttcctggcat cctccaggaa atttccgccc
      661 cgttcgtaag ccatttccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      721 gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc cgcctttttt
      781 ctcctgccac atgaagcact tcactgacac cctcatccgt gccaacatag taagccagta
      841 tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc gccaaaagcc
      901 tgacgcgcct gcggcttgtc caaccccgcg ccgaccggga aaggctttcc cgcccgtccc
      961 ggcgttatca ggaggccggt ttgtcagaga aacggagcaa gatgctcacc atgtgggtga
     1021 cggaggacga gcaccggcgg ctgctcgagc gctgtgacgg caggcagctg gcggcgtgga
     1081 tgcggcagat ctgcctggac gaaaagccgg cacgttccgg aaagctcccc tcactctcgc
     1141 cggcgctgct gcgccagctt gccggcatgg gcaataacct gaaccagata gcccgccgtg
     1201 ttaacgccgg cggcggcacc ggccatgaca gggtacagat tgtggcggcg ctgatggcca
     1261 tcgatgccgg ccttgagcgg ctgcggcacg ccgttcttga gaaaggaacg gacgatgatc
     1321 gttaaatttc atccgcgcgg ccgcggcggt ggcgccgggc cggtggacta tctgctgggt
     1381 aaagaccggc agcgtgaagg cgccaccgtc ctgcagggga agccggaaga agtccgggag
     1441 ctgattgacg cgtcgcctta cgttaagaaa tacacctccg gcgttctgtc gtttgcggag
     1501 gccgatctgc ctcccggcca gcgggaaaaa ctgatggcca gcttcgagcg ggttctgatg
     1561 cccggactcg ataaagatca gtacagcatt ctgtgggtgg agcacgcgga caaggggcgt
     1621 ctggagctga actttctgat ccccaatacc gagctgctga ccggcaggcg gctccagcct
     1681 tactacgacc gcgccgaccg gccgcgcatc gatgcctggc agactatcgt gaacggcaga
     1741 ctggggctgc acgacccgaa cgcgccggaa aaccgccggg cgctggtcac gccgtccggg
     1801 ctgccgaaaa cgaagcagga ggccgcagaa gccattaccc ggggactgct gacccttgct
     1861 tcgtccgggg agctgaaaag ccgtcaggat gtcactgaca ctcttgaagg tgcaggtttt
     1921 gaggtggtgc gcaccacgaa aagcagtatc agcattgccg acccggacgg ggggcgaaac
     1981 atccgactca agggagccat ctatgagcag tcttttaacg ctggcgaagg acttagagca
     2041 gcaatcgaaa gcgcagcagc agagtaccgg cgagatgctg aaagccgcat tcagcgagca
     2101 cgagcagtct gtcagagcgg aactgagcgc aagcgcgagg agaatcagcg acgccatcct
     2161 cgcccacgag cagagtatga gcgaggcgat ggagaaaaac cggcggagcg tactgcggac
     2221 tgccgggcgg acatggctca ccatcctgat ggtgtcagcg ctgctgatcg gcacgagcgg
     2281 gagcattctg tggtggcagg ggcagcagat aaccgacaat tacacgcatc ttcgtcagca
     2341 ggaagatacc ctggcgaaaa tgaccgcccg gacgtgggga gtgcgctatc aggagagcag
     2401 cgacggccgg aggtttctta tcctgccgcc ggggatgcaa accgaagcca tcccctacga
     2461 cgggacgaca tggatccgcc tgaaacagga gtagcggaaa atgacagagc tggaaaagca
     2521 gttgctgagc gcattagagc agctacagca ggactactcg caaaggctgg acgaatggga
     2581 gagcgccttc gcggaatggc ggagcatgtg tgggcttatg caacgggaga acgcgatgct
     2641 gaacgagcgc gtgtcggact tgagcacgca ggtgctgagt ttaagcgagc agctgcgccg
     2701 cttgtccggg aactgaatgc catcgagctc cggcagaggg agaatgagta tcagaaaaca
     2761 ctggagctgg agcgccagcc gcagaaaacc taccgcgggc cgacacttta aaccatccag
     2821 gcagctgaca acccgcacga agaggggctc ttatttctcc cgctaaatag gttcgtgatc
     2881 aaaaagatca cggcctatac ctgggggaaa ttatgaaatt gcttaacgtc attgaagctc
     2941 tacttaactg cctgtatctg gcggccgagc tgtgggactg gctggagaag catttacctg
     3001 tttttaaggc gttggcgctg attttagtgt ggctttacac gccatgcatc tggctgcatg
     3061 gctatgcagt cgggtaaaat ttatctgtcg cgatcggggt ggtttaccgg gtgggttgtt
     3121 gttggttttg gctgactgct gccccgttcg cggcgaagct gtccggggcg gtgcgggcaa
     3181 cggatgttat ggtaaataag agcgaccgtc ccggtcgctc ttgaaagaga attcggcctt
     3241 aggtcagttt tcgcagatac aatcatgtgg actgcggaga cattcttcgc actcctcaca
     3301 tatgcactca tccagttttt caccgcactc gtcgcaacgt gagcagctac agtgctcatc
     3361 taaatttcca caatcgctgc agcgctcaca gacgcagtct gccagcttct cgttgcagtc
     3421 atcgcagaag ccacaggtac aggcgctggc agggagggca caaacatcgc aaatcttatc
     3481 cattggatcc attttatctc acttttattt tatcagtggc tgtgtgcggg cgatttcccc
     3541 agcgcgatag atgtggattt aagtgccatt tttcaatgat taaggcagct aaaaaagtgg
     3601 ttttttaggg cttttcaacg tgaccagcac tgacggggat aaatacaggt aagcaataat
     3661 gctcagtcat gaaaaggaga atccgatgga gcaacatacc cctgaatact tacgacgtac
     3721 cttagctcat aaccgtgccc taatggacga tatcattagc agtggcatgt ccaggtacta
     3781 caacacggag attgtggatg ccgcatgtga ggcgatagaa gctgaattgc gacgacgagg
     3841 aattttgtaa gagacaaagc ccgcttatgc gggctttgtc gttactggcg cttctgttac
     3901 tcagtgttgt ctggggttcg gtagatctgt agccagtacc tgccaggtgc gctcgttggt
     3961 ggtgcgaact cattatcgcg aatggtgcag tatcgtccat atttgcgggt aacagtatga
     4021 gtgtttgtgt tgatgtcaca ttcttggaat gagccaccat cacgacctat tttgccatgt
     4081 ggtcctacat aaattgccat tttgttctcc tttttagagc cttgtatggg catcttattg
     4141 gtgttcaaac aacaatcatt tcaagatcta agaactgaac taaaaatcaa actaaaggta
     4201 ggtatgcggg ggttttacgg caaaaagccc gcacgagcgg gcttttactt atggaaacat
     4261 cggatgaagg tgttctagcg tctcggataa agtcttacga cttttcgaga ggctgtactg
     4321 agcctgcata ttaacccacg actctgcaga gcggcctata actttagata gtcgaagagc
     4381 catctcataa ctaagatcag tttttccatt gagcaatcta cttaatgcag attgtgatac
     4441 ttcaagctta tcagctaact ctgcggcagg tatttcgaac ggttccatga acatttccct
     4501 gatgaattca ccggggtgta caggatcgta aagatttatc atgccttcct ctattggttt
     4561 tgtatttgat tacacaaaac attttgtttc tttcgcgttg cgcattgcac aatacgcaag
     4621 tgtctgttat tgacaaaaaa agcagtaagc tttaggatgc tcggtggagc agaaagctac
     4681 cgctgatatg gtgtctttac cgactttttc aaggcgccat attgtacggt tagcgctctt
     4741 ggaagtcaaa cgaggtaatc gacctcagag acaaagttac gtcaattatt ttaataatgc
     4801 aaggggttca aatggacgct gtcaaattgt tttctgttta tagagataga tatcgacgaa
     4861 aattttatgg agatgattga tgataaaaaa cataaagcat aaagggcttc ggagttttgc
     4921 tgaaaacggt aacaccgccg gaattcagcc tcacttagcg aaaaaacttc gcatgatatt
     4981 gactacgcta agcaccgcaa catgtcttca agatattgtg gatgtaaggg cgctggggtg
     5041 tcacccccta aaaggtgaac gtaaaggtga gttttccgta gaggttaacg gaaactggag
     5101 ggtggttttc aaggtggaag aaccaaacgt cttagacgtt gatcttgaag attaccatta
     5161 gtaatcaata agtaagttac taatccatcc tagtggcaat atggatggat tagggacttt
     5221 attcaaaaat aagtacctct ggaggattta tgagagcctt tatgattttg ttgttccctt
     5281 tgctttgtgt agtcattgct ggggttcttg ctgttcgtgg cctagatggg tggggatggt
     5341 tcctgtttgc agctgttttg tgtactggtg ttgatacggt gcagaaacat gtctctaaca
     5401 agaataattc t
//
