LOCUS       NZ_CP072864             2462 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Escherichia coli strain 184/2aE plasmid pEc184_6.
ACCESSION   NZ_CP072864
VERSION     NZ_CP072864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18630606
            Assembly: GCF_018361365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Santos Tufic-Garutti,S.D., de Araujo Longo,L.G., Fontana,H.,
            Garutti,L.H.G., de Carvalho Girao,V.B., Fuga,B., Lincopan,N., de
            Pinho Rodrigues,K.M. and Moreira,B.M.
  TITLE     OXA-181 carbapenemase carried on an IncX3 plasmid in high-risk
            Escherichia coli ST167 isolated from a traveler returning from
            Sub-Saharan Africa to Brazil
  JOURNAL   Diagn Microbiol Infect Dis 102 (1), 115570 (2022)
   PUBMED   34739936
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   dos Santos Tufic-Garutti,S., de Araujo Longo,L.G., Fontana,H., Groto
            Garutti,L.H., Brigido de Carvalho Girao,V., Fuga,B., Lincopan,N., de
            Pinho Rodrigues,K.M. and Meurer Moreira,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2021) Medical Microbiology, Universidade Federal
            do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, CCS, Bloco I,
            Lab I2-59, Rio de Janeiro, Rio de Janeiro 21.941-902, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-MAR-2020
            Assembly Method                   :: UNICYCLER v. 0.4.8
            Assembly Name                     :: Ec184_2aE
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:00:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,845
            CDSs (total)                      :: 4,719
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 71 of 233
            Pseudo Genes (incomplete)         :: 162 of 233
            Pseudo Genes (internal stop)      :: 43 of 233
            Pseudo Genes (multiple problems)  :: 42 of 233
            Pseudo Genes (short protein)      :: 4 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072864.1.
            Bacteria and source DNA available from Laboratorio de Investigacao
            em Microbiologia Medica, Avenida Carlos Chagas Filho, 373, CCS,
            Bloco I, Lab I2-59 Cidade Universitaria, Rio de Janeiro, RJ,
            Brasil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="184/2aE"
                     /isolation_source="Stool specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc184_6"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.95 S 43.17 W"
                     /altitude="15 m"
                     /collection_date="2017-12-10"
                     /collected_by="Instituto de Microbiologia Paulo de Goes"
     gene            1090..1632
                     /locus_tag="J9158_RS23935"
                     /old_locus_tag="J9158_23820"
     CDS             1090..1632
                     /locus_tag="J9158_RS23935"
                     /old_locus_tag="J9158_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001696621.1"
                     /translation="MNIDEVAKWRPFTDQDGNIWDLSFLDAHEVSYTHCSPGKPDIVYK
                     FTVSYSFHCFCKDYPEQTEEEKLSLMYDAGKDSRPFCKVRYALARDKLRAIIEGLGNAK
                     VIHAGYGSYAFVEITVDEGESEFYFVPFKVYRERKKLRLHVTSAYPVKERPSGKPVSFF
                     KIAHNLLRGKPLPKPHM"
                     /name="hypothetical protein"
     gene            complement(2178..2261)
                     /locus_tag="J9158_RS24360"
     CDS             complement(2178..2261)
                     /locus_tag="J9158_RS24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058652899.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="hypothetical protein"
     Typing          complement(3..178)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.709"
                     /coverage="94.0"
                     /evalue="9.01e-51"
     Typing          complement(1962..2443)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.706"
                     /coverage="94.0"
                     /evalue="1.33e-148"
ORIGIN
        1 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cagcgccgca tccttttcct
       61 gccctgcctg tcacgccgta tgcctttgtc cttatccgtg tgcaggtaac gctcgccgca
      121 gccgaacgac cgagcggagc gagtcagtga gcgaggaagc ggaatatctg cgggcttctc
      181 tttggcaccg tacgcgccat agctgcattt tttaatacga tgcaggatag ggcgaacgcc
      241 gcaaagtgac gtcacctgac gttcctggat tacaaaggtt aaagcagcgg acaggaaatg
      301 ttttgtgccc acagctatgc tggcttattc acaaaacagc gggacaagat gtgtttttgg
      361 agtaccgccg gcccgctaaa gactcctggt tagctcccgc atgtttgcta tctttttgct
      421 atctttttgc taccattttg ctactttttt gctatctcca gagtcttctg gtctttcggt
      481 tgctatcctt ttgctacttt tttgctatca aaatgctacc tctcccttct tgcaataaat
      541 gaccaaggca cgttagcgat gtatacatcg ccgcgccgca agggtgcccc ttccgaatcc
      601 cgttcatcac tgcgtgctga ttcatccacg ttaaatccga cccccttagc aataaatcca
      661 gccatgcctg acggcctggc caacgcctca cggccgcaca gtaaactgcg cacccgtgag
      721 gcgttatcgg ggtggttcgt ggtggctttc agggcgttca tcggatttta gccgctaaaa
      781 tcccggccag gtatgtccgt gcatagccat gcagtcgcgc cagaatcatc aggggcgttt
      841 tttctcgata tgggcacaga aaggtgtgtg tttcaggtcg gaggcctcag aaacgcgctg
      901 agggcctctg cggcggtgca cgcaacggat gttatggtaa ataacactta atcattacca
      961 atgtatccat gaaaggttgt ggcgaagtct tctgtaaaag gcctagctct tattttgcca
     1021 cgctccttcc gcttgtttct tctctggtag aatgtgcgga ttctgccatc cggatattga
     1081 ggttttttcg tgaacattga tgaagttgct aagtggcgtc cttttacgga tcaggatggc
     1141 aatatatggg atctgtcatt tcttgatgca catgaggttt cttatactca ttgttctcct
     1201 ggtaaacctg atattgtgta taagttcact gtttcttact catttcattg tttttgcaag
     1261 gattatccag agcagactga ggaagaaaaa ttatcgttga tgtatgatgc aggcaaagac
     1321 tcgaggccat tttgtaaggt ccgatatgcc cttgctcgag acaagctgag agccatcatt
     1381 gaaggattgg gtaacgcgaa ggttatccat gctggatatg gtagttatgc ttttgtagag
     1441 attactgtag atgaaggtga aagtgagttc tactttgtgc cttttaaggt atatcgtgag
     1501 cgtaaaaagc tgcgcttgca tgtaacaagt gcttatcctg tcaaggagag accgtcaggt
     1561 aagccagtta gctttttcaa gatagctcac aatctcctgc gcgggaagcc attacctaaa
     1621 cctcatatgt aacaaagccc gcttagttgc gggcttcgcc agaaatttct tctggtattc
     1681 agaacacctt taccgggctt ggcctcgacc aacattgtta ttggtagtgg gatgtctggt
     1741 gttaccagtc tgctgcatcc ttaaagcaat tgtaggtagt gtgatgtaga tttgcaacca
     1801 acattttggt tgcaacctat atactaattt ttctcgataa tgccggaatc cattgctgtg
     1861 tctggctttc ttgaagctcc tgactataga taccctatat cctaacagtt attctgctgt
     1921 ggatatcaga aatgaacact tgagatcatc agtggctcgt cgtagtctct tgctcagaaa
     1981 cgaaaaaacc accctggcag gtggtttttc cgaaggttag ttaatcctgg cagattctct
     2041 aaccgtggta acagtcttgt gcgagacatg tcaccaaata ctgtcctttc agtgtagcct
     2101 cagttaggcc gccacttcaa gaactctcgt tacatctctc gcacatcctg cttaccagtg
     2161 gccgttgcca atggcgatta gtcgtgtcta tccgggttgg actcaagacg atagttaccg
     2221 gacaaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     2281 actgcctacc cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg
     2341 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc caccaggggg
     2401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     2461 tt
//
