LOCUS       NZ_CP072863             3423 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Escherichia coli strain 184/2aE plasmid pEc184_5.
ACCESSION   NZ_CP072863
VERSION     NZ_CP072863.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18630606
            Assembly: GCF_018361365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Santos Tufic-Garutti,S.D., de Araujo Longo,L.G., Fontana,H.,
            Garutti,L.H.G., de Carvalho Girao,V.B., Fuga,B., Lincopan,N., de
            Pinho Rodrigues,K.M. and Moreira,B.M.
  TITLE     OXA-181 carbapenemase carried on an IncX3 plasmid in high-risk
            Escherichia coli ST167 isolated from a traveler returning from
            Sub-Saharan Africa to Brazil
  JOURNAL   Diagn Microbiol Infect Dis 102 (1), 115570 (2022)
   PUBMED   34739936
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   dos Santos Tufic-Garutti,S., de Araujo Longo,L.G., Fontana,H., Groto
            Garutti,L.H., Brigido de Carvalho Girao,V., Fuga,B., Lincopan,N., de
            Pinho Rodrigues,K.M. and Meurer Moreira,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2021) Medical Microbiology, Universidade Federal
            do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, CCS, Bloco I,
            Lab I2-59, Rio de Janeiro, Rio de Janeiro 21.941-902, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-MAR-2020
            Assembly Method                   :: UNICYCLER v. 0.4.8
            Assembly Name                     :: Ec184_2aE
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:00:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,845
            CDSs (total)                      :: 4,719
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 71 of 233
            Pseudo Genes (incomplete)         :: 162 of 233
            Pseudo Genes (internal stop)      :: 43 of 233
            Pseudo Genes (multiple problems)  :: 42 of 233
            Pseudo Genes (short protein)      :: 4 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072863.1.
            Bacteria and source DNA available from Laboratorio de Investigacao
            em Microbiologia Medica, Avenida Carlos Chagas Filho, 373, CCS,
            Bloco I, Lab I2-59 Cidade Universitaria, Rio de Janeiro, RJ,
            Brasil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="184/2aE"
                     /isolation_source="Stool specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc184_5"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.95 S 43.17 W"
                     /altitude="15 m"
                     /collection_date="2017-12-10"
                     /collected_by="Instituto de Microbiologia Paulo de Goes"
     gene            <1..183
                     /locus_tag="J9158_RS23915"
                     /old_locus_tag="J9158_23800"
     CDS             <1..183
                     /locus_tag="J9158_RS23915"
                     /old_locus_tag="J9158_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_213201130.1"
                     /translation="QRREGFRKAEASLRLEGMDPSGVPRYECLKTRIISGETSYEQGRK
                     EILDYYLRINHKGEE"
                     /name="antitoxin VbhA family protein"
     gene            complement(200..910)
                     /locus_tag="J9158_RS23920"
                     /old_locus_tag="J9158_23805"
     CDS             complement(200..910)
                     /locus_tag="J9158_RS23920"
                     /old_locus_tag="J9158_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053272217.1"
                     /translation="MLYWKKMPSLWIGNRLTEFNSLETSHAIAAIKIYITFCLFGKEED
                     DGVRTVKLTFTEISEIASISRSLVNEGLKLLDNKKLIKYLSPASKRKKIYTVDVDGCIH
                     DGWCKLPMAGIVSEDRTISAFQSMHNRYHFELVALQLYLYLLYARDNFQEHVLARKQTI
                     CEKLACRNVDLNKAITYLIHIGLLKGIKQKAVESYPIELFHDSFYFYVKTGASNALTFK
                     KQPLVNPPEKEIPY"
                     /name="hypothetical protein"
     gene            complement(1255..1776)
                     /locus_tag="J9158_RS23925"
                     /old_locus_tag="J9158_23810"
     CDS             complement(1255..1776)
                     /locus_tag="J9158_RS23925"
                     /old_locus_tag="J9158_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657475.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_099368844.1"
                     /translation="MDNSQIGTPESHEQDNYNFVQVSRAYLKEWRALTRKSPIASEILM
                     YLIENMGRTTNAVVCSYTTLMEITGVSRASVARAVKILKEDRWMQAVKIGNATAYCVNA
                     KAFWQAGRNQKKYAIFQATVIASSSEQESDFLEKSQQDLRYIPFVDLKDRPIVGTDELP
                     PPDQTDLDLD"
                     /name="replication/maintenance protein RepL"
     gene            2370..3338
                     /gene="mobV"
                     /locus_tag="J9158_RS23930"
                     /old_locus_tag="J9158_23815"
     CDS             2370..3338
                     /gene="mobV"
                     /locus_tag="J9158_RS23930"
                     /old_locus_tag="J9158_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053272216.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADADRTPENHCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     Typing          2362..3233
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.189"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 cagcgccgtg aagggtttcg aaaagcggaa gcatcactaa gacttgaggg aatggatcct
       61 agtggagtac ctcgttatga atgtttgaaa acgaggatta tctctggcga aacaagttat
      121 gaacaagggc gtaaagaaat attggattac taccttagaa taaatcataa gggcgaggag
      181 taattcgccc tcaggtatgt cagtacggaa tttctttttc tggagggtta acaagaggtt
      241 gctttttgaa tgtaagcgca ttgcttgctc ctgtttttac ataaaaataa aatgaatcat
      301 gaaaaagttc tatgggataa gactccactg ccttttgttt aattcctttt aaaagaccta
      361 tgtgtattaa atatgttatt gctttattta aatctacatt gcggcaagca agcttttcgc
      421 aaatagtttg ttttctagct aacacatgct cctggaagtt atctctggca tatagaaggt
      481 ataaatatag ctgaagcgct acaagctcaa aatggtatct attatgcatt gactggaagg
      541 cgctgattgt tctgtcttcg ctaacgatgc ctgccatagg cagcttacac catccgtcat
      601 gaatgcaccc atcaacatcc actgtataga ttttctttct ttttgacgcg ggcgataggt
      661 atttaattag ttttttatta tcgagcaatt tgagaccttc atttaccaaa gatcggctga
      721 tagaagctat ttcgcttatt tctgtaaacg taagttttac ggttcgcact ccatcatcct
      781 cttcttttcc aaagagacaa aaagttatat atatctttat ggcggcaatg gcatgtgaag
      841 tctctaaact attaaactct gtcagtctat taccgatcca gagtgatggc atttttttcc
      901 aatataacat tagtgattcc tttatgcgga aatgaaagtg aaataaatca taattaacac
      961 acaaatggcc aatgcaagac tagtaagtga ccctgccact tttcccagcc tttcagcaag
     1021 ctctacaaat cgctctaagt cacccatttt gacctctgct cctgtttacc gatataccga
     1081 ttatcccagc aatccgcgta tctgtatcta taggagcaaa aatctatcta gaaagatatg
     1141 ggaaaaatta atatgaattt ttataattaa atcaaaaagt taacatcaaa aaatggttat
     1201 caaatgaact tcaatatggt tcacggtgaa ccatatttga aggttttcag aggctcagtc
     1261 gagatctaga tctgtttgat ccggaggcgg gagttcatcg gtgccaacaa tagggcggtc
     1321 tttgagatcg acaaaaggaa tatatcgaag atcttgctgt gatttttcta agaaatcaga
     1381 ctcttgctct gaggaggagg caatcacggt agcttggaag attgcgtatt ttttttgatt
     1441 tctacctgcc tgccaaaatg ctttggcatt aacacaatat gctgtggcat tacctatttt
     1501 tactgcctgc atccatcgat cttccttcag gattttaacc gcccttgcta ctgatgctct
     1561 agagaccccg gttatctcca tcaaagtcgt atagctgcaa acaacagcat tagttgtcct
     1621 acccatattc tcaatcaagt acataaggat ttcagaggca atagggcttt tccttgtcag
     1681 cgcacgccat tccttgagat acgcgcggct aacctggacg aaattatagt tatcctgctc
     1741 atgagactct ggagtgccaa tctgtgaatt atccattgct ctatagacca tttcaatatc
     1801 ccatttgata ccggttgtat cattagagat gcaacaggtc aacttgagtc tcatagctag
     1861 tgagactcat aagtctcatg gctggtgaga ctcccccttt ttttaaccca atataatcag
     1921 cgatttactg aaagctcctt ttctatatct attaagtccg tgcatacgta gcccaatgta
     1981 cgcctagtag acattccgct tcctcgttca ggctgttcgt tcctcgctgc gctgcggtac
     2041 gcctcagcct gacctgcggc gagcggcacg tgctctttca aagacgactt tgttgcctga
     2101 cattcgtcaa gggcgagcgt agcgagtgcc atttaccctt gacgactcac cggataaaac
     2161 aggcttggca gtggtggggg gtggcgcgga gcgacgcctt ccacctgcca gaccgctgtt
     2221 gaacttgacc tttaggcagc ttgctgcccg aacgattttg taaagcaaag tctagtgagt
     2281 acactatcat cctgatagtg gcccgccgga ggcatcgcaa tgcgatcccc cgtctggccg
     2341 gatggacgcc ggaacgaggg gggggaagca tggcgaacta cgcgataatg cgctgtaaaa
     2401 agctgacggg aatgggcagt gtggccagcg cgttacagca ctgctacagg gagagagaaa
     2461 cgccgaatgc tgatgctgac cggacaccag aaaaccactg ctctgtgtct cagtcggcag
     2521 atgaggcgat gggaaagctg cgggagctgt tgccggaaaa acgccgtaaa gatgcggtac
     2581 tggctgttga atacgttatg acagcaagcc ctgagtggtg gaacgaggcc acaccgcggc
     2641 agcaggcgga gtttttcgcc cgttctgagc aatggctgga gaacaaatac gggaaagatc
     2701 gcgttgtggc ggcggtggtt cacagggacg aggccacccc gcacctgtct gcgttcgtgg
     2761 tgccattgac gcaggatggg cggctgtccg cgaaagagtt tattggcggg cggtcaaaga
     2821 tgcgtgacga tcagagcacc tacgctgaat cggtaaaaaa actgggtctt gagcgcggca
     2881 tcgagggaag ccgtgccacg catcagacag ttcagcacta ctacgaatcg ataaaccgtg
     2941 gcacccgtag ccaggtatcg atctcaccgg aggcgctgga gccgcgtgta ctgcggaagg
     3001 gtattttcac caaagacgtg gaagatcagg cagccatagc caaacgttta agccaggcag
     3061 tgaacgatgg ctttgcagga accatcgcta tggcctctca gagcgcccag aatgccaaaa
     3121 gagcgcgcga tctacagaag actatggatt cgcagcaaaa acgcctacag agcgttacag
     3181 agccttttaa aggactttcc cgggagcaga tgacccatat tctgacgatg gcgcagacgt
     3241 tccagcaaca gaatcacgat cgtgaaaaac agcgccagct tgaacgggag caggaacgcc
     3301 agcaacgtca gaaaactgac agaggtatta gccgatgagt gaaaagcagc gccagcttga
     3361 acgggagcag gaacgccagc aacgtcagaa aactgacaga ggtattagcc gatgagtgaa
     3421 aag
//
