LOCUS       NZ_CP072805             1943 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Escherichia coli strain PK5074 plasmid pPK5074-2kb, complete
            sequence.
ACCESSION   NZ_CP072805
VERSION     NZ_CP072805.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18615003
            Assembly: GCF_017903455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Li,R., Mohsin,M., Lu,X., Abdullah,S., Munir,A. and Wang,Z.
  TITLE     Emergence of Plasmid-Mediated Resistance Genes tet(X) and mcr-1 in
            Escherichia coli Clinical Isolates from Pakistan
  JOURNAL   mSphere 6 (4), e0069521 (2021)
   PUBMED   34431695
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Mohsin,M., Li,R. and Lu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2021) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: 1-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017903455.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:32:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,282
            CDSs (total)                      :: 5,163
            Genes (coding)                    :: 4,886
            CDSs (with protein)               :: 4,886
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 78 of 277
            Pseudo Genes (incomplete)         :: 191 of 277
            Pseudo Genes (internal stop)      :: 50 of 277
            Pseudo Genes (multiple problems)  :: 36 of 277
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072805.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PK5074"
                     /isolation_source="Urine sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPK5074-2kb"
                     /geo_loc_name="Pakistan: Faisalabad"
                     /lat_lon="31.26 N 73.4 E"
                     /collection_date="2019-12-17"
                     /collected_by="Dr. Mashkoor"
     gene            complement(918..1031)
                     /locus_tag="J8617_RS26415"
     CDS             complement(918..1031)
                     /locus_tag="J8617_RS26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_095470903.1"
                     /translation="MHEPPVQSDRCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(919..1400)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.938"
                     /coverage="92.0"
                     /evalue="3.51e-174"
ORIGIN
        1 tcacatcctt tagacgttgc gatgcaggat agggcagacg ccgcaaaatg acgtcatttg
       61 cggtatctgc cccgaaaaca gaatgttttc tcctgagcct gcagctggct ctcagaaccc
      121 ctaccgcctc gcaaagctcg ttgctcaacg cctcacgacc gcacagtaaa ctgtgcaccc
      181 gttcaccgtt atcgatgggg ttcagcgagc cttttgagca gttcgcagga ttttagccgc
      241 taaaattccg gccaggcatg ttcgtgcata gccatgcaga tgcgcagaaa tcatcaggcg
      301 cgtttttcag gggttggtgc tgggaaaggt gccggaaacg gcccgttcgc ctcaaaatgg
      361 ctctgaggcg ttttcagcgg tgcgggcaag ggatgttatg gtaaatatcg tttatgttga
      421 ttaacaaatt aaaagataag attattccta cttctctatt ttctggggct tttatgaaaa
      481 aggtaattct ctcattggtt tttctgtcat cagtaattct tgctggctgc tcaagccaag
      541 caggtccatt tgtgaccaac attagcagtg atggtagagg tgggcttgtc gttgagaaat
      601 gtatggttcg ccttgataga atgattaata ccgttgaatc atctagctgc actaattcaa
      661 gtattactct gtcctcacca tcttctagct cgacactgac tcgttagatt tcatgttgct
      721 acttgagatt atttaattca gcgggtagtc tttcgcttga aaaagaaaaa accgccctgc
      781 aaggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg
      841 gaggagcaca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg cataacttca
      901 agactaactc ctctaaatca attaccagtg gctgctgcca gtggcgcttt tgcatgtctt
      961 tccgggttgg actcaagtcg atagttaccg gaacaggcgc agcggtcgga ctgaacgggg
     1021 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     1081 ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     1141 aggagagcgc acgagggagc taccaggggg aaacgcctgg gatctttata gtcctgtcgg
     1201 gtttcgccgc cactgatttg agcgtcagat tttgtgatgc ttgtcagggg ggcggagcct
     1261 atggaaaaac ggcaacgccg cttccttttc tcttcctttc ccaatttgat tgctactagc
     1321 ctactccttt acttctgagc cggtaacgcc cgccgcagtc tcacgtccgg agcgtagcga
     1381 ccgagtgagc gaggaggcgt aatttcatcg actcctgcat ttttcactta cgccctacag
     1441 ctacattttt cacctgccag gggtcagttt ggatatagag aattattgta cggtaagcct
     1501 gttttttgaa cagacttacc tatagcaata gggtgtactc ccgcgctata cggcattttc
     1561 agaggtttga ccttaatcgg ataacctaaa tgcgatactg caagagcgac aacttaaaag
     1621 cgatagccac tgacgcaaaa tcaacccctt acgaaccaag attggttaaa taatgcgctt
     1681 aacgtacaaa aatttccgat ctccaaactg accccttctt cgcctcttat gtttctcgaa
     1741 tgaatcgatg gggacaggaa atgttttgtg gccaccgccg cttcgccggc accacaaaac
     1801 tgtcggacaa gatgtgtttt tggagtaccg ccggcaccaa aaacatttca tcaggcgcag
     1861 cctgataccc tgaatttcgt acgtacgaaa ttttgctttt gttcagatcg agggcaggat
     1921 tcgcaaagtg ctggcgtttg tgc
//
