LOCUS       NZ_CP072715             6304 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Enterococcus faecium strain VB3378 plasmid pVB3378_p8, complete
            sequence.
ACCESSION   NZ_CP072715
VERSION     NZ_CP072715.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533778
            Assembly: GCF_017898025.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB3378
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB3378
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.4x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017898025.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 02:51:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,196
            CDSs (total)                      :: 3,103
            Genes (coding)                    :: 2,982
            CDSs (with protein)               :: 2,982
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 40 of 121
            Pseudo Genes (incomplete)         :: 73 of 121
            Pseudo Genes (internal stop)      :: 28 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072715.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3378"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB3378_p8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            253..654
                     /locus_tag="J7155_RS15740"
                     /old_locus_tag="J7155_15715"
     CDS             253..654
                     /locus_tag="J7155_RS15740"
                     /old_locus_tag="J7155_15715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182486065.1"
                     /translation="MEEFTACYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEV
                     QDNYERTKKDVQYFKDTQTEEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQA
                     TNIQNPTKKETALNYLESVKEWLDQQQQN"
                     /name="hypothetical protein"
     gene            1537..2487
                     /locus_tag="J7155_RS15750"
                     /old_locus_tag="J7155_15725"
     CDS             1537..2487
                     /locus_tag="J7155_RS15750"
                     /old_locus_tag="J7155_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            2508..3041
                     /locus_tag="J7155_RS15755"
                     /old_locus_tag="J7155_15730"
     CDS             2508..3041
                     /locus_tag="J7155_RS15755"
                     /old_locus_tag="J7155_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            3057..3227
                     /locus_tag="J7155_RS15760"
                     /old_locus_tag="J7155_15735"
     CDS             3057..3227
                     /locus_tag="J7155_RS15760"
                     /old_locus_tag="J7155_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            3677..4210
                     /locus_tag="J7155_RS15765"
                     /old_locus_tag="J7155_15740"
     CDS             3677..4210
                     /locus_tag="J7155_RS15765"
                     /old_locus_tag="J7155_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            4222..4425
                     /locus_tag="J7155_RS15770"
                     /old_locus_tag="J7155_15745"
     CDS             4222..4425
                     /locus_tag="J7155_RS15770"
                     /old_locus_tag="J7155_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            4747..5127
                     /locus_tag="J7155_RS15775"
                     /old_locus_tag="J7155_15750"
     CDS             4747..5127
                     /locus_tag="J7155_RS15775"
                     /old_locus_tag="J7155_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5109..6023
                     /locus_tag="J7155_RS15780"
                     /old_locus_tag="J7155_15755"
     CDS             5109..6023
                     /locus_tag="J7155_RS15780"
                     /old_locus_tag="J7155_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5983..6261
                     /locus_tag="J7155_RS15785"
     CDS             5983..6261
                     /locus_tag="J7155_RS15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     Typing          1539..2487
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5110..6020
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtattttagg aattatttgg cttagctata aggcttatca aacaagatat tcattaaaag
       61 gctctaaaat tcgatttaag cgatttttat tggtcggggt tataatttgc cttatcaatc
      121 gttttaagtg gtttattcgg cgttttagag ggtaataaac gtgtttcaag aagcatttta
      181 ttaggggata tcactcaaaa gtatgaagca gcatgtgata aaaagaaaaa agagcaagca
      241 ttagcacaaa aaatggagga atttaccgct tgttatgagg acatgaacaa tatttttgtg
      301 gatcaagaga aacgtttaac agataaaaac atgaaaaaac tcactcgttt ttatcaaaac
      361 ttaccagaaa aacagcaaaa agaggttcaa gacaactatg agcgaaccaa aaaagacgtg
      421 caatatttta aagatacgca gacagaagaa gcttgttgta acttatttag cgatacaatc
      481 cctttttcaa ccagtgaaca agaacgaaaa gaacggcaac aatcggtcac atacgaacgt
      541 tatgaaacac tgcttcagca agcaacaaac atacaaaatc caacgaaaaa agaaacggca
      601 ttaaattatc tagaaagtgt caaggaatgg ttggatcagc aacaacaaaa ttgaatttga
      661 ttagcaatca tgttaaactg aacctgcaaa tgatcaaaca ttagatacac gaaagcccca
      721 gagaggtcgg attctctggg gctttttttc aggttgatgt cgcctttatt gattttactt
      781 cttgtttcgg ttattcaacc aataactgaa taaagagaca caaacttcta caagaagggg
      841 gcaaatgaac cgttcaaaca ttagatacac ctccttccag tcgcaagact gtcggatagg
      901 cgacaagtta ttatacctta caaaataaaa tctgtgtggt gtttttaagt atcccttatt
      961 ttcgggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaagaaca agataaggtt
     1021 ttttacccaa gactgtctta aaacgactta gacgagctat cagaggcttc ccccacctcc
     1081 gttactgact tgtttttagc aaaggagcct ttaggcgact gcgcagtaca atgtgagagc
     1141 atagcgatca cactttttta gcgaccgtag ggagcaaggt tttgaattgg cagttttgaa
     1201 aaatgcgcga acctgttggt agaaaaaaga agtggttttt aggcgggaaa ttttgtcacc
     1261 ctcttttata atctcttttt aaaccttttt ttaaacctct ttaccgtcgg ctgtgtcctt
     1321 actccttcaa tggatacagc gttttataaa ctacattttg tctgctttta aactacattt
     1381 tgtctgcttt taaactacat tttgtctgct tttaaactac attttgtctg cttttaaact
     1441 ctttttttgc actcacgtat gttataatat gcattatcac agacaaaacg ttgtttataa
     1501 cgtctgtatc cattgaagga gtaaggagga atatctttgg caaatgaaat cgtgaaatac
     1561 catcatgaat taaataccat tccgttgcgg aaatttacac cagttgaaat gaatctattt
     1621 ttctctattg tgtctagaat gcgcgaaaaa ggcgataaaa cagtccgtct tacttttgac
     1681 caactaaaag atttaagtaa ttataaagca actgctaata atcgtttcat taatgattta
     1741 gaaatgactt atgataaatt aatggattta cgttttggta aaagaagtgc taatgggctc
     1801 gatatagata gattcgttat gtttagtgag tttaaaatac gtggaactgc taaagatcca
     1861 tatgtagaca tacaagttta cgaaaaagct ttacctttac taaacaattt agatgagtgg
     1921 gttcgctatt cactccaaca atttaacgaa ttacaaagta gctatgcaaa gactatgttt
     1981 cgattactaa aacaatatcg aactaaaggt tttgcttatt tttctaaaga ggattttcat
     2041 gaactattag atattccaaa aagttactgg aacaaccaag caaacgttga taatcgtgtt
     2101 ataaagccta tacgacagga attaaccgca atcttcaaag gattatcaat caaaaagaaa
     2161 tacggaaaag ggcgaggaaa acctgtcgtt ggctatcaat ttacgtttaa accagaaatc
     2221 aaaaaagcag atgattttaa taaacatgca ccaaccaaag aagaacaaaa taaagcattg
     2281 gctttagctg ataaacaaaa agaatacgaa gaacctgcta caacaattaa cgaaaaaatc
     2341 aaacagaaga aacgagaact tgaactcctc caacaaatca aacaactaga aaaagagaaa
     2401 gaagctcttg aaaaacaaac agagtccgca ctttcgaaaa aagagaaaaa aagtttatta
     2461 tctgatttag ataatttatt ccagtagcaa cctaggaggc aaccagtatg caaccacaag
     2521 aaacctttac aataaaggaa ttgtcagaac tttttaagat gagtaggcaa gcaatatcga
     2581 aacatatcca aaaattagat tcttccatga tagctaaaaa tgagcgtggt tataaggttg
     2641 tattgcgttc tggagtattg caacttgctc gcaacttagg caaccaaatg ttgctagata
     2701 ggttgcacga gtacgaacaa gacaacgata aaaatgttaa tataccaagc aaaaacgtgg
     2761 ttacaaacaa gttgctagat cagttgcgtg aagaaaatga atttttacga gaacaacttg
     2821 ccctcagaga gcaaaataat tctcatatac aaaaactact cgaccagcaa caacaactca
     2881 ccctacaagc caacaaacaa atcgaaaaac tgcaagaaaa actacagttg acttatacag
     2941 aagaagctcc agagaatgac tcaacgactc tttctgaagc aacagaaaca atagacaaac
     3001 aaattgatac tgaaaaaaaa tggtggcaac tttggaaata actggaggga acattcatga
     3061 caagaaaaaa tcttctattc ggttctattt tactaggaat tctaggattc agtccgatgt
     3121 tattgtcagt gatcaataaa gggaataaac atggtgaatt gaaattgaat ccaaattatt
     3181 acaacagtcc taacgaagaa ttgtttgtaa aagaaacaga taaataataa aaaagactgc
     3241 tctttcgagc agccagttaa aaaataatga gtgtttattc ttgaaaaagc aacgcgctta
     3301 tgaagcgcta ccaaaaattt atcatatttt attaaaaata acaacccagt aaaaaatgac
     3361 tggtaaaaat tcacaaagaa acggaaagaa gcaaccgcag aaaatcaagc ttaaaaagag
     3421 cttttgacct agaccaatcg aaaatcacaa agtgagcctc tgtatcggtt gatacaaggg
     3481 ctacttggca tagtgtacat tatgtcaaca agaatttaat ataacatatt ttcctaatgc
     3541 attctaacta taccacatta tcaaaaatat aaattatctt tactaaataa aatattgaca
     3601 agcctatacc ttttgtttta tgattttcac aaagaaaacg ttttctatat atttttctgt
     3661 atcaggagga aattctatga aaaaatttac tttaggggta gctttgttaa ctattggggc
     3721 agttgtttta ccgtcaacta ctatagttta tgcttcagaa aattcccaag tggtatcatt
     3781 aaacgaaaac aatgctactg aaaatgtagt tattgagtac actgaagatg gtatggtcgt
     3841 aactaaatat gaggatggtt atagtgcgga agattttgca aattcttcag atatcatgac
     3901 aagagctgca gttgcaagat ggggcaaaaa ggaatataca aatattgctg taaccactgg
     3961 agtagctgct aatgctatta atgctgcctt ttgggcagga gggaaagcta gcacttctat
     4021 gtttggtatc ccaggttggg caattggtgc tttgttaaca ggtgcaagtt ggactaaaaa
     4081 aggtgcttct ccagggaatg cagttgcaaa agaatgggac aaaaatggta atggctggat
     4141 tggattttat taccaaaggg gatatgatgc agctggaaga gttgtagcta ctagatattc
     4201 aacgttataa gttaaggagt gatgtttttg atatctaaat cattaaaatc attaaaaaca
     4261 tttactaaaa agaatccgca aatagctact attcgtattt ttatatttgc tatagctgtt
     4321 gctataattg ttaatatgct aagcggaggg caattataca aatctggttt atttttagtg
     4381 ttaatcttag ttgtcttaga cataataaag tctaaaaaaa aataaatcga tacattttag
     4441 aagaataggg ttgtttgaat attacaaagc aacaatttca ccttcggtga aataagcgaa
     4501 actttacaaa aagtggctag caaatgctag cctttttttg tattgtggag cgtggatttt
     4561 gagggggatt caagggggag cttgcgctcc tttgacaagc ggttatttgc accaaatacg
     4621 gaggtatttg gctagcaaat atagagcttg ccaaaccctt ttagtgagct ttactcattg
     4681 taccttttta ggtacaatgg tattgcgaac gatacgtccg taggacgtac tgaaagggtg
     4741 ggacttatga gcgaacaaca tcaaaatttg gctagcgatc attctaaaaa atatacctat
     4801 cgatccgagc caaaacaaat cagttttcga gtgagcgaat ccgaatttgc aaagctgaag
     4861 cagtcagctg aagctttgca aatgagtgtg ccggctttcg tgaaagccaa ggcacaaaac
     4921 gcgcgtctgg tgacaccgaa agtggcgcca gacattgccc aggcaatcgc gcgcgattta
     4981 gccaaagctg gagggaatat caatcaaatt gccaagtggt gcaatacaca ccaacacgac
     5041 gttgctcctg atgatgctca acgtttatcc gaaaacctaa aaatcatgca aaaggagcta
     5101 caaaagatat ggcaacagtt aaagtaagtc gaaccacttc atgtagccga gcaatcaatt
     5161 acgcagaacc acgagcaacc gtcaaaacag gaataaactg tgacattgat tatgccaaaa
     5221 gtgaaatgaa acaaatacgc acgctttatg gaaaagacga tcgtgtacaa gcacacttat
     5281 tgattcagtc ctttcgacca ggagaaatta ccgctgaaaa agccaatcaa ctaggggcag
     5341 agtatgcgaa aaaaatagcc cctaaccacc aaatcgcgat ttacacacat acagacaaag
     5401 accatattca taatcacatt gtcattaact ccgttaatct tgaaactggg aaaaaatttc
     5461 aagcccatgg gcaagcattt ttaaacaaat gttatgacct aaatgacgag atttgtcttg
     5521 aacatgactt aacaatcacg gaacgaggaa agaaaaccga aaaacgaacc atgtctgaaa
     5581 ttaaattaaa agaaaaaaat gaaccggttt ggaaagacga aattcggttt gcgattgatc
     5641 aaacaatgaa aaacaaaaaa actcgaacgt atacccaatt ttgtgattca cttaaaacat
     5701 tcggcattca ttgcttgaat cgcggaaaaa attttactta cgaattggtc cacaaaaaga
     5761 aaaaagtacg atccaacaaa ctaggaaagg attacgaaaa ggagacgatt ttacatgagc
     5821 tggacagacg agaacgaacc tacaatcaac aacgaaccaa cgagctacga agagctcgag
     5881 ctagcattcg cgaaactgca gaagaagtac gaagagaaca acgaactttt acgcctgaac
     5941 aatcggaaat tagaccgact cgaagaaacc atgaacaaca cgttgaacga ggaacagacc
     6001 accaacaaga attatcgctc taaaacgtta aaagacatcg aaaacgaaat aaagagacaa
     6061 cgtgcctttc ttgcgcctta tacccaagaa aggatacaaa ctacgatgga ttcacaagtc
     6121 aaaaaactcc accaagcgct attagaccag cgaaaccaag aacaaaaaaa ggctttaatt
     6181 caacgatcga tggatattct tgccccctta tgttgcttag tactaacatt attagccttt
     6241 tggttggtat ggggagaata aacagacatg gcattctatt taccctatct actcatttct
     6301 gcca
//
