LOCUS       NZ_CP072706             5216 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain IMBL-B9 plasmid pKBT5216, complete
            sequence.
ACCESSION   NZ_CP072706
VERSION     NZ_CP072706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18221107
            Assembly: GCF_023921665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Park,M.G., Choi,J.Y., Kim,J.H., Park,D.H., Wang,M., Kim,H.J.,
            Kim,S.H., Lee,H.Y. and Je,Y.H.
  TITLE     Isolation and molecular characterization of Bacillus thuringiensis
            subsp. kurstaki toxic to lepidopteran pests Spodoptera spp. and
            Plutella xylostella
  JOURNAL   Pest Manag Sci 78 (7), 2976-2984 (2022)
   PUBMED   35419912
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Park,M.G., Choi,J.Y. and Je,Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) Department of Agricultural Biotechnology,
            Seoul National University, 1 Gwanak-ro, Seoul 08826, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023921665.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 14:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,736
            CDSs (total)                      :: 6,585
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 118 of 328
            Pseudo Genes (incomplete)         :: 239 of 328
            Pseudo Genes (internal stop)      :: 81 of 328
            Pseudo Genes (multiple problems)  :: 86 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072706.1.
            Bacteria and source DNA available from Laboratory of Insect
            Microbiology and Biotechnology, Seoul National University, Republic
            of Korea.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="IMBL-B9"
                     /db_xref="taxon:1428"
                     /plasmid="pKBT5216"
                     /geo_loc_name="South Korea: Suwon"
                     /collection_date="2018-04-13"
     gene            237..860
                     /locus_tag="J2N67_RS33670"
                     /old_locus_tag="J2N67_006749"
     CDS             237..860
                     /locus_tag="J2N67_RS33670"
                     /old_locus_tag="J2N67_006749"
                     /inference="COORDINATES: protein motif:HMM:NF037852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_278003246.1"
                     /translation="MNKFNKCNHIPFPCAFPPAGQGPTGVTGPTGITGPTGITGLTGVT
                     GPTGVTGPTGVTGPTGITGSTGPNIPPTFANVAFQGTQTVDVFTPIVFNVTQSISNMTF
                     VNNTSEIIVNEPGIYKIEYFVTTATTNIPPIQFTFFVNGILLVASSMGIVTAGGEVSNT
                     IFRTLNTGDTIEVFNTSGTPIIIPAIVVGGGLRQSARISVHRIF"
                     /name="hypothetical protein"
     gene            1447..1743
                     /locus_tag="J2N67_RS33520"
                     /old_locus_tag="J2N67_006750"
     CDS             1447..1743
                     /locus_tag="J2N67_RS33520"
                     /old_locus_tag="J2N67_006750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042595346.1"
                     /translation="MSKQQVKFYVTEKEMERLKFLSEMRYMTVPNYAKLTALGVQIRQP
                     KEIVVGNVELQEEDKAVLKEILERRRGISYIAYDTGFNQRLEQLAKRLLGEEK"
                     /name="hypothetical protein"
     gene            2157..3473
                     /gene="mobV"
                     /locus_tag="J2N67_RS33525"
                     /old_locus_tag="J2N67_006752"
     CDS             2157..3473
                     /gene="mobV"
                     /locus_tag="J2N67_RS33525"
                     /old_locus_tag="J2N67_006752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_097939008.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLRLEEQKRYFES
                     SLAFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKAKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQVFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNVAVIIKNDYERLQKTDLVKENKQLHE
                     AVDEICDSLQDSQKRNLKLQEENKQLSTEVSSLKAHIRDLQINIKVLYQQTKKVFKEQF
                     KAFRGLIKNELDIKGVDNQFEREHAREVKSRQKGYDMER"
                     /name="mobV"
     gene            4496..5080
                     /locus_tag="J2N67_RS33530"
                     /old_locus_tag="J2N67_006753"
     CDS             4496..5080
                     /locus_tag="J2N67_RS33530"
                     /old_locus_tag="J2N67_006753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033344.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_042595349.1"
                     /translation="MANSIDFKQVEKNARLREIESSKILSEEEMYLANDLQAKANSKGM
                     KLVPERKIKNNFRFAQFIQANWSYLRDIKYLTNAEKTFLIDILSNIGFLSNCIVDDVQK
                     KNPTPLNQQAIADLLGTYKPNVSRTVKKLVDKGVLCKAELGLEENNARAYSLFVNPNII
                     FSGNKDEVNQTLQAMFSKVPKELKKLPQRLF"
                     /name="replication/maintenance protein RepL"
     Typing          2158..3470
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttttaaac ttttaatagc ttgtccctct tactctatat atagaaagct aaaagttccg
       61 caaggaacag gtcgtatttt tgagtaaaaa gcactcaaaa atctccacct tcatcataaa
      121 acacgatcaa aaccataaat aaaactaata caaacctttg tcaaatttat ttcataaaca
      181 tattaagtat gtatctattc aattaattgc aattcatttg aaaagttggt gaaaacatga
      241 acaagtttaa taaatgtaat catattcctt tcccatgtgc gttccctcct gcagggcaag
      301 gacctacagg agttacagga ccaactggaa ttacaggacc aactggaatt acaggactaa
      361 caggagttac aggaccaact ggggttacag gaccaactgg ggttacagga ccaactggaa
      421 ttacaggatc tactggacca aatattccgc caacatttgc taacgtggct ttccaaggta
      481 cccagacagt tgacgtattt actccaattg tatttaatgt aactcaaagt atttctaata
      541 tgacatttgt aaataatact tccgaaatta tagtaaatga accaggaatc tataaaattg
      601 aatattttgt tactactgct actaccaata ttcctcctat acagtttact ttttttgtta
      661 atggaattct attagttgct agtagtatgg ggattgttac agctggtggt gaagtttcta
      721 atactatttt tagaacatta aatactggag atactattga agtatttaat acaagtggaa
      781 caccaataat aattccagct atagtagtag ggggcggcct taggcaaagt gctcgtatat
      841 ctgttcatcg tattttttaa acaatacgca aatttcaaac gctaagtata gttttagtac
      901 atggtttgtt agtctcaata tattatgtaa tagccctttt ttaagggcta tttttttaat
      961 tttaatagtt gtctaatctg acaactatta aaacctctat aaacccttaa tataataagc
     1021 tttttaaata gtttagtagt cgttttactc tcactctcta tataaaagct aaaagttccg
     1081 caaggaacag gtcgtatttt tgagtaaata ccactcaaaa atctccacct tgtccgcttt
     1141 gctccctcct tgccaaactt ttttcggctg gtttttagct ttcgccaagc cgaacagacc
     1201 aaaaacgggg ttaagttgct cgacttggac tagcaattgt accagccgaa ccgccagttc
     1261 ttccgttcaa agtatgattc ttttttgata cgaatcaacg ctatgtgggg tttagccccc
     1321 acacgactag ccagcactca cacccaaaaa caggggaaag ataattctaa cgttcaaatt
     1381 agtactaaaa aggtctataa ttaatctatt aaaatagtac tatttagttt attggaggtc
     1441 gtgtgaatgt ctaaacaaca ggttaaattc tatgttacgg aaaaggaaat ggaacgtttg
     1501 aaatttttgt ctgaaatgcg atatatgaca gttcctaatt atgcaaagtt aacagcatta
     1561 ggtgtgcaaa ttagacagcc aaaagagata gttgtaggta atgtagaatt acaagaagaa
     1621 gataaagcgg tattaaaaga aattttggag agaagacgag gaattagcta tatagcttac
     1681 gacacaggat ttaaccagcg attggaacaa ctcgcaaaac gactattggg agaagaaaaa
     1741 taagcggtaa gtgtcaatgg taagcggact ttcagggatc agcggtttgt gctgatccct
     1801 gaaaggagca ttaaggaatg gacacgtgca gctgattctt catcctcttg gcagtttctc
     1861 aaaacgaatc gttttgggga cgccagccgg cgaggaagcc cctcaaggat tgaggggttg
     1921 gggagttcga ttgatggggt caaggggaag agttcccctt gtggggtgtg gggcaaagct
     1981 ccagggattt tatcacgcca tggcgtgatt tggcgaagcc aacaaggtct cgcagagccc
     2041 acaccaaatg gaatttggta tagtgggcta taccaaaact ttggattcgc tctctcgata
     2101 tgttatgata gtttcaaaag tagtcctctt accgaagaca attgtgaggg aaaaacatga
     2161 acaaattcgc aatcattcac atgcagaaat ttcaaatttc tgatgtgcaa gggattcaaa
     2221 aacacaacca aagacaagga aaaagtaaat ccaatttgga tattgattat tcgaaaagtg
     2281 aacaaaatta tgatttgttg aatcagcaaa aaattagata tgaaagcaca attaaacaag
     2341 agatcagtga gcgagtaaaa cgaaaacctc gagcaaattc cgttgtactt tctgaatttg
     2401 tagtaacagc ttctcctgat tacatgcatt cattacgttt agaagaacag aaacgttatt
     2461 ttgaaagttc gctagcgttt attcaaaaac gttatggaaa acaaaatacg ttgtatgcga
     2521 tggttcatat ggatgaagcg acaccacata tgcatattgg tgtcatgcca attacagaag
     2581 acaatcgttt gtcagcaaaa gatatgttta cgagaaaaga gctgatatct ttgcaacagg
     2641 actttccgtt ggaaatgcgg gaaaaaggtt ttgatgtaga gcgtggagaa ggttctgaga
     2701 aaaaacattt atcgcctcaa gcctttaagg aaaaacaaga tttagaagta gaagtggaac
     2761 agttatccaa tgtaaaaacg catttaaagg caaaagtagt ggaaactcat aatcaattac
     2821 aacaaaccac aaactacatt gaaaaacaga atgaaacatt acaaaaaatt cagcaacaat
     2881 ttttaagttt agataaaaaa ataaaagaaa agaaacaggt atttgaaacg tttcgaaacc
     2941 aaattccgga caagccagtg tcaatgtctt atttgcgaga agaaacaaaa acggaagtaa
     3001 caacaaaact gtttggaaag ccagaaatca cagagaaaaa aacaggaaac atcgtggtta
     3061 cacgtgagca atggagagat atgacagaaa aagtgaatgt agcggttatt ataaagaacg
     3121 attatgaacg tttgcaaaaa acggatcttg taaaagaaaa caaacaatta catgaggcag
     3181 ttgatgaaat atgcgattca cttcaagatt ctcaaaagag aaatttaaag ttacaagagg
     3241 aaaacaaaca attaagtaca gaagtaagct cgttaaaagc tcatataaga gatttgcaga
     3301 ttaatataaa ggttttatat caacagacga aaaaagtctt taaagagcaa tttaaggcgt
     3361 ttagaggact tattaagaac gaattggata taaagggtgt agataatcaa tttgaacgtg
     3421 aacatgcaag agaagtaaaa agtagacaga aaggatatga catggaacgt tagaaaaagg
     3481 tatgttgtaa aaaagagcat aaggaaaccg aatcccactt tgtataaaaa gtggtgaaat
     3541 aggctgtgcc tattgggttt ctaaagattt ctagctggta cgttttttct atgacctgtg
     3601 gtcgtctagt ggtcgtttat agctgttctg tggccttcca gtggcctttg gtggtcattt
     3661 agtggctgtt taaaatgtac taaaaattta aatatgctac aataaatatc catcacaaat
     3721 aactttttaa aggttacatc taaaaaatct ctttgtatat ttatatataa agagattttt
     3781 tacgtttatg taattgaaat tatgttaaag ttaataagac tacatttgag aatgtgggtt
     3841 caagttgctg tttgactgaa ttgcacaaaa aaaaccaatt ccattacacg ggaattggtt
     3901 tttttttgtg ccttttataa accaatacat tatatgtgca aatagataaa aagtttttat
     3961 catgtttaaa aatataaaag gtaaatacaa aaagcctagt aattactagg ctttttgtga
     4021 aaaaagaaat accattttca ccttaagcca tttattagtt tcacattatt ttatgtctat
     4081 tttaaataaa aagttcgaaa gtaaactttt gaatagaagt ttactttcaa actattgatt
     4141 tcattggatt ataaggaagt tttagaagaa tagatatttc cgtaaggaaa gtatacattc
     4201 attatgctga aataaaagga tttacccgta attctcttgc ccccaagttt ttgaataagc
     4261 aagagaatta cgggtgttta tttgtttttg taaatataaa aagtcagaca agatacaaca
     4321 aaaaaagcca ttcatgctat aattggagtg tcggctacaa ctatacatga agggcttttt
     4381 tgtctctatt taataatatt agagttttta aaatttataa gaaaacttct aattacataa
     4441 taacaaaaga aaaggctctt cgctagttgt aaatactaga aacgaggaga agaacatggc
     4501 aaattctatt gattttaaac aagttgagaa aaatgcgaga ttaagagaga ttgaaagttc
     4561 taaaatttta tcggaagaag aaatgtattt agctaatgat cttcaagcaa aagcgaattc
     4621 taaaggaatg aaattagtgc cagaaagaaa aattaaaaat aatttccgct tcgctcaatt
     4681 tattcaagca aattggtctt atttaagaga cattaaatat ttaacaaatg cagaaaaaac
     4741 atttttaatt gatatattat ctaatatagg ttttttatct aactgtattg tagatgacgt
     4801 acaaaagaaa aatcctactc ctttaaatca acaagctatt gctgacttac taggaactta
     4861 taaacctaat gtaagtagaa ctgtaaaaaa attagtagat aaaggtgttt tatgtaaagc
     4921 agagctagga cttgaagaaa ataacgccag ggcatattcg ctttttgtta atccaaatat
     4981 tattttttcc ggtaataagg atgaagttaa tcaaacttta caagctatgt tttcaaaagt
     5041 ccctaaagaa ttaaaaaaat taccccaacg attattttaa gagcctttgg ctcttttttt
     5101 tattgacaaa aataaaaagt atatcttgtt ttaaaaatcg tagttagcaa ttttgctaac
     5161 taatgttagt aaaattgcta actacggaaa acttaaaaac ccttgatatg actatg
//
