LOCUS       NZ_CP072705             5535 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain IMBL-B9 plasmid pKBT5535, complete
            sequence.
ACCESSION   NZ_CP072705
VERSION     NZ_CP072705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18221107
            Assembly: GCF_023921665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Park,M.G., Choi,J.Y., Kim,J.H., Park,D.H., Wang,M., Kim,H.J.,
            Kim,S.H., Lee,H.Y. and Je,Y.H.
  TITLE     Isolation and molecular characterization of Bacillus thuringiensis
            subsp. kurstaki toxic to lepidopteran pests Spodoptera spp. and
            Plutella xylostella
  JOURNAL   Pest Manag Sci 78 (7), 2976-2984 (2022)
   PUBMED   35419912
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Park,M.G., Choi,J.Y. and Je,Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) Department of Agricultural Biotechnology,
            Seoul National University, 1 Gwanak-ro, Seoul 08826, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023921665.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 14:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,736
            CDSs (total)                      :: 6,585
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 118 of 328
            Pseudo Genes (incomplete)         :: 239 of 328
            Pseudo Genes (internal stop)      :: 81 of 328
            Pseudo Genes (multiple problems)  :: 86 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072705.1.
            Bacteria and source DNA available from Laboratory of Insect
            Microbiology and Biotechnology, Seoul National University, Republic
            of Korea.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="IMBL-B9"
                     /db_xref="taxon:1428"
                     /plasmid="pKBT5535"
                     /geo_loc_name="South Korea: Suwon"
                     /collection_date="2018-04-13"
     gene            join(5321..5535,1..82)
                     /locus_tag="J2N67_RS33480"
     CDS             join(5321..5535,1..82)
                     /locus_tag="J2N67_RS33480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728519.1"
                     /translation="MAKEQIKLYVSEEEKERLELLAKMNSTTVSNYVRMTALGVRVRPP
                     KEIPVVVEKERLEDEDKLVLQELLERTQGKKDFITLDREFNTRLIALAGRLLE"
                     /name="hypothetical protein"
     gene            488..1813
                     /gene="mobV"
                     /locus_tag="J2N67_RS33485"
                     /old_locus_tag="J2N67_006743"
     CDS             488..1813
                     /gene="mobV"
                     /locus_tag="J2N67_RS33485"
                     /old_locus_tag="J2N67_006743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_011839880.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLRLEEQKRYFES
                     SLVFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVDRGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMKKKVDAAVIIKSDYERLQKTDLVKENKELHS
                     AVDEICDSLKESQKRNVGLQKENKQLSTEISSLKAHIRDLQTNIKVLYQQTKQVFKEQF
                     KTFRGLVKNELVGREVENHFEREHERENKKKISRHRGYDMER"
                     /name="mobV"
     gene            2061..2513
                     /locus_tag="J2N67_RS33490"
                     /old_locus_tag="J2N67_006744"
     CDS             2061..2513
                     /locus_tag="J2N67_RS33490"
                     /old_locus_tag="J2N67_006744"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728522.1"
                     /translation="MKKKEILEVTEMLSTFIDAGAGAQELMKAITERNLHKNSNFIFVF
                     HTLKAWIESDNPEVKLSKTEKAVLFTLLPYVGWNNQIELLQPTMQKLCGYKDRSYFSKV
                     LNSLVRKNVLEKQSVGKQNYWFFNSLLVKKGNSKERKPYIKFELKD"
                     /name="hypothetical protein"
     gene            3105..3569
                     /locus_tag="J2N67_RS33495"
                     /old_locus_tag="J2N67_006746"
     CDS             3105..3569
                     /locus_tag="J2N67_RS33495"
                     /old_locus_tag="J2N67_006746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011839882.1"
                     /translation="MLKIKSKFKKIIPATMAFAALLTVAPLSSFAAETETPVKNNVVNF
                     PQTNYKVENVNQTVSLDEDGDVSAQGWKKSAVVWGLKNGGDALQTLTKWLGAGTKEAKY
                     LNKHAKSIANALDRFESHIEQNLIDFMIFECGIPGGSARVIASAITGLVL"
                     /name="hypothetical protein"
     gene            3593..3991
                     /locus_tag="J2N67_RS33500"
                     /old_locus_tag="J2N67_006747"
     CDS             3593..3991
                     /locus_tag="J2N67_RS33500"
                     /old_locus_tag="J2N67_006747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1878 family protein"
                     /protein_id="WP_021728521.1"
                     /translation="MNIEQEISKLKYHKELMLTMLLHENPDKFSFYHQIINHDLEKKDE
                     IAILNIISLFNKRLSNENTFDFEKEFFDSISLQVIYDCNVKPTIEEYEGYLKAINIPIN
                     PKYLLMAINKQKESDNVCEFLLEKYKEK"
                     /name="DUF1878 family protein"
     gene            4038..5159
                     /locus_tag="J2N67_RS33505"
                     /old_locus_tag="J2N67_006748"
     CDS             4038..5159
                     /locus_tag="J2N67_RS33505"
                     /old_locus_tag="J2N67_006748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="WP_021728520.1"
                     /translation="MKKFIMIAVLLFGLCSGSVTSFASELSKKDDYLIMFRDKIDENLI
                     EKQGGEVKETYSNLPIVKAEFPYDPTKTLKQYFEVLEVEQDGNVKANGTKQSLHNGVSS
                     VLSTKGKGVSPLTGKGIAVAVLDTGIDREHPDVKIKDGVSFVENVLDFDDDNGHGTHLA
                     GIIGAQKNNSGMTGIAPDVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHKANIILMSLGG
                     KKESVFFEEAINKAYEKGILLVSSAGNEGYKEGDSITYPAKFNSVIAVGALDAKDQRGF
                     LSSKGEQLELMAPGVDIWSTWNDGSYRFDSGTSMAAAHVAGVASLLLENDRMLSNQQVR
                     ELLKKSATPLGSPFEYGNGKINLSHALQMMNQK"
                     /name="S8 family peptidase"
     Typing          489..1810
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaaggaaaa aaagatttta ttacactaga tagagaattt aatacacgtt taattgcact
       61 agctggacga ttactcgaat aaagaggaaa ttttgaggta agtgtcaacg gtaccggacc
      121 gagcccgaaa gggatcggga ggataaggag tggacacgta cgtcagatgt atcaccctct
      181 tggcagttcc tcaaaacata ttgttttggg gacgccaacc ggcgagggag cccctcaagg
      241 attgaggggt tggggagttc gatggatggg gtcaagggga aaagttcccc ttgtggggtg
      301 tggggcaaag cccctagcat ttgtcacgct atggcgtgat ttggcgaagc caacaaggtc
      361 tcgcagagcc cacaccaaat gtaatttggt atagtgggct ataccaaatc tttggattcg
      421 ctctctcaat atgttatgat agtctcaaaa gtagtcctct tacggaagac aattgtgagg
      481 gaaaaacatg aacaaattcg caatcattca catgcagaaa tttcaaattt ctgatgtgca
      541 aggaattcaa aaacataatc agagacaagg aaaaagtaaa tccaatttgg atattgatta
      601 ttcgaaaagt gaacaaaatt atgatttgtt gaatcagcaa aaaattagat atgaaagcac
      661 aattaaacaa gagatcagtg aacgagtaaa acgaaagcca cgagccaatt cggttgtact
      721 ttcagaattt gtagtaacag cttcgcctga ttacatgcat tcattacgtt tagaggaaca
      781 gaaacgttat tttgagagtt cactagtgtt tattcaaaaa cgttatggga aacaaaatac
      841 gttgtatgcg atggttcata tggatgaagc gacaccacat atgcatattg gtgtgatgcc
      901 aattacagaa gacaatcgtt tgtccgcaaa agatatgttt acgagaaaag aattgatatc
      961 tttgcaacag gacttcccgt tggaaatgcg ggaaaaaggt tttgatgtag atcggggaga
     1021 aggttctgag aaaaaacatt tatcgcctca agcctttaag gaaaaacaag atttagaagt
     1081 agaagtggaa cagttatcca atgtaaaaac gcatttaaag acaaaagtag tggaaacgca
     1141 taatcaatta caacaaacca caaactacat cgaaaaacag aatgaaacgt tacaaaaaat
     1201 tcagcaacaa tttttgagtt tagataaaaa aataaaagaa aagaaacagg aatttgaaac
     1261 gtttcgaaat caaattccgg ataagccagt gtcaatgtct tatttgcgag aagaaacaaa
     1321 aacagaagta acaacgaaac tgtttggaaa gccagaaatt actgagaaga aaacaggaaa
     1381 catcgtggtg acacgtgagc aatggagaga tatgaaaaaa aaggttgatg cagcggttat
     1441 tataaagagc gattatgaac gtttgcaaaa aacggatctt gtaaaagaaa acaaagaatt
     1501 acatagtgca gtcgatgaga tatgtgactc acttaaagaa tctcaaaaga gaaatgtagg
     1561 gttacagaag gaaaacaaac aattaagtac agaaataagc tcattaaaag ctcatataag
     1621 agatttacag acaaatataa aggttttata tcaacaaacg aaacaagtct ttaaagagca
     1681 atttaagacg tttagagggc ttgtaaaaaa tgaattggtt ggaagagagg tagaaaatca
     1741 ttttgaacgg gagcatgaac gtgaaaataa gaagaaaata agtagacatc gagggtatga
     1801 catggagcga tagaagacat tcaaaaagta aataaaactt ttgggaaact ttatttgaca
     1861 atcttttgag agaatatgtg tgataaaatt aaaaaacgag ccatgctcga acatggctcg
     1921 tttcaaaggg tatttcagcg aaaataccaa taacttgttg tcgtatatag attatcataa
     1981 ttttattatt agggtaatac ctgaattatg atttttggta tttttgttgg tgctcggaat
     2041 attgagagga gcaacaacaa atgaaaaaga aagagatttt agaagttaca gagatgttga
     2101 gtacttttat tgacgctggt gcaggtgctc aagaacttat gaaggcgatc actgaaagaa
     2161 atctacataa gaattcaaat tttatatttg tttttcatac gttaaaagct tggattgaaa
     2221 gtgataatcc agaagtgaag cttagtaaaa cagagaaagc agttttattt actttgctac
     2281 cttatgtcgg ttggaataat caaattgaac ttttacaacc aactatgcaa aaactatgtg
     2341 gttataaaga taggtcatat tttagtaagg ttttaaattc attagtaagg aaaaatgttt
     2401 tagaaaaaca aagtgtagga aaacaaaatt attggttttt taattcttta cttgttaaaa
     2461 aaggtaattc taaagaacga aaaccatata taaaatttga actaaaagac tagtaaaaat
     2521 tactagtctt ttttatttac atattttccc aatatgtggt ttttacccca catatatgtg
     2581 gtttttaccc cacatattgg gaattaatta caatcgctag aacccctgat ataatcatgt
     2641 ttattcactt ttttattaaa aattccatgt tgttctttct atatctatat aaggggttgg
     2701 tttttctcca tacaatggtc aggtagtatt ttttgaaaaa aactctcaaa aaatctccac
     2761 ctttcttcca ttgcattcca aaattcggct ggtttttagc gttcgccaag ccgaacagac
     2821 caaaaacggg attaaattgc tcgacttgga ctcgtaatta taccagccga accaccaagt
     2881 tctatcgttc caagtgataa acttatccga taataaatta cgttcttggg ggctagcccc
     2941 caaaccccta gccagcactc acacccaaaa acagggaaac tcaacattgg attactaatt
     3001 ttgttttcca aaagtatatg caattttaca tatacagaaa aaagagtttt gaaatatgac
     3061 atacttatct ttgttaaaaa aatttatttc tggaggaatt atacatgtta aaaattaaat
     3121 ctaaattcaa aaaaatcatt ccagcaacaa tggcatttgc ggctctttta acagttgcac
     3181 ctttatcttc ttttgcagct gaaacagaaa cgcctgtaaa aaataatgta gtgaatttcc
     3241 cacaaacaaa ttataaagta gaaaatgtaa atcaaactgt ttctttagat gaagatggtg
     3301 atgtatcagc tcaagggtgg aaaaaatcag ctgttgtttg gggcttaaaa aatggtggag
     3361 atgcgttaca gacacttaca aaatggttgg gtgctggaac taaagaagca aaatatttga
     3421 ataaacatgc gaaaagtatt gctaatgcat tagatcgttt tgaaagtcat attgaacaaa
     3481 atttaattga ttttatgatt tttgaatgcg gaattcctgg tggatcagct cgagttattg
     3541 caagtgcaat tactggtctt gttttataat tattgaattg taggtgttta atatgaatat
     3601 tgaacaagaa atttctaagc taaaatatca taaagaatta atgttaacta tgcttttaca
     3661 tgaaaaccct gataaatttt ctttttatca tcaaattatt aaccatgatt tagaaaaaaa
     3721 agatgagata gcaattttaa atattatttc tttatttaac aaaaggttat ctaatgaaaa
     3781 tacatttgat tttgaaaaag aattttttga ttctattagt ttacaagtaa tttatgattg
     3841 taatgtaaaa ccaactattg aggaatatga aggttattta aaggcaataa atattccgat
     3901 aaaccctaaa tatttattaa tggcaattaa taagcaaaaa gaatcagata atgtttgtga
     3961 atttttatta gaaaaatata aagaaaaata aggtgctttt gcaccttatt ttttatgtgt
     4021 aatggaggat caaaaatatg aaaaaattta ttatgatagc tgtattgtta tttggactat
     4081 gcagtggaag tgtaacttct tttgcgagtg agttatcaaa gaaagatgat tatttaatta
     4141 tgtttcgaga taaaattgat gaaaatttga ttgaaaaaca aggtggggaa gtaaaagaaa
     4201 cgtattctaa tttacccatt gtaaaagctg aatttcctta tgatccaaca aagactttga
     4261 aacagtattt tgaagtcttg gaagttgaac aagatggaaa tgtaaaagca aatgggacga
     4321 aacagtcttt acataatggg gtatccagtg ttttatctac gaaaggaaaa ggagtttccc
     4381 ctcttactgg gaaaggaatt gctgttgcgg ttttagatac tggtattgat agagagcacc
     4441 cagatgtaaa gataaaagat ggcgtttcat ttgttgaaaa tgttttagat tttgatgatg
     4501 ataatggtca tggaacacat ttagcgggta ttattggagc gcaaaaaaat aatagcggta
     4561 tgacaggaat tgctcctgat gtggatttat atgcagtgaa agtattagat aagtatacga
     4621 atggaaaata tagtacagtt gtaaaaggaa tcgattgggc aattgaacat aaggcgaata
     4681 ttattctaat gagtctaggt ggaaaaaaag aatctgtatt ttttgaagaa gccattaata
     4741 aagcatatga aaaaggaatt ttgttagttt catcagcagg gaatgaagga tataaagagg
     4801 gagattcaat tacttatcca gctaaattta attctgtaat tgcagttgga gcgttagatg
     4861 ctaaagatca acgagggttc ttatctagta aaggagaaca gttagaatta atggcaccag
     4921 gtgtagatat ttggagtact tggaatgatg gttcttatag atttgatagt ggtacttcaa
     4981 tggcagccgc tcatgtagct ggtgtggcat ctttgttatt agagaatgat agaatgttgt
     5041 ctaatcaaca agtaagagag ttattaaaaa aatcagcaac tcctttagga agtccatttg
     5101 aatatggaaa tgggaaaata aatttaagtc atgctttgca aatgatgaat caaaaataaa
     5161 agagaaccaa ctttatagtt ggctctcttt tattttttaa aggggtatag aagggataag
     5221 tattaaaggg tataaaaagg actataaatt atatattgaa aaagggtata taaagggata
     5281 tgatgaaagg aacaaaatat accctaagag gatgttaaaa atggcgaaag aacaaatcaa
     5341 attatatgtt tctgaagaag aaaaagaacg attagagtta ttagctaaaa tgaatagcac
     5401 aacagtttcg aactatgtaa gaatgacagc tttaggtgtt cgtgttagac cacctaaaga
     5461 aattcctgta gttgttgaaa aagaaagatt agaagatgag gacaaattgg tgttacagga
     5521 attgcttgaa cgaac
//
