LOCUS       NZ_CP072704             7241 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain IMBL-B9 plasmid pKBT7241, complete
            sequence.
ACCESSION   NZ_CP072704
VERSION     NZ_CP072704.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18221107
            Assembly: GCF_023921665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Park,M.G., Choi,J.Y., Kim,J.H., Park,D.H., Wang,M., Kim,H.J.,
            Kim,S.H., Lee,H.Y. and Je,Y.H.
  TITLE     Isolation and molecular characterization of Bacillus thuringiensis
            subsp. kurstaki toxic to lepidopteran pests Spodoptera spp. and
            Plutella xylostella
  JOURNAL   Pest Manag Sci 78 (7), 2976-2984 (2022)
   PUBMED   35419912
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Park,M.G., Choi,J.Y. and Je,Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) Department of Agricultural Biotechnology,
            Seoul National University, 1 Gwanak-ro, Seoul 08826, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023921665.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 14:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,736
            CDSs (total)                      :: 6,585
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 118 of 328
            Pseudo Genes (incomplete)         :: 239 of 328
            Pseudo Genes (internal stop)      :: 81 of 328
            Pseudo Genes (multiple problems)  :: 86 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072704.1.
            Bacteria and source DNA available from Laboratory of Insect
            Microbiology and Biotechnology, Seoul National University, Republic
            of Korea.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="IMBL-B9"
                     /db_xref="taxon:1428"
                     /plasmid="pKBT7241"
                     /geo_loc_name="South Korea: Suwon"
                     /collection_date="2018-04-13"
     gene            complement(1104..2420)
                     /gene="mobV"
                     /locus_tag="J2N67_RS33450"
                     /old_locus_tag="J2N67_006735"
     CDS             complement(1104..2420)
                     /gene="mobV"
                     /locus_tag="J2N67_RS33450"
                     /old_locus_tag="J2N67_006735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_001031678.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIIKSDYERLQKTDLVKENKQLHE
                     AVDEICDSLQDSQKRNLKLQKENKQLSTEISSLKAHIRDLQINIKVLYQQTKKVFKEQF
                     KAFRGLIKNELDMKGVDNQFEREHTREIRSRQKGYDMER"
                     /name="mobV"
     gene            complement(2834..3130)
                     /locus_tag="J2N67_RS33455"
                     /old_locus_tag="J2N67_006737"
     CDS             complement(2834..3130)
                     /locus_tag="J2N67_RS33455"
                     /old_locus_tag="J2N67_006737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000043574.1"
                     /translation="MSKQQVKFYVAEKEMERLKLLSEMRFMTVPNYAKLTALGVQIRQP
                     KEIILGDVELQEEDKAVLKEILERRRGNGYIAYDIGFNERLEQLAKRLLGEEK"
                     /name="hypothetical protein"
     gene            complement(3672..4286)
                     /locus_tag="J2N67_RS33460"
                     /old_locus_tag="J2N67_006738"
     CDS             complement(3672..4286)
                     /locus_tag="J2N67_RS33460"
                     /old_locus_tag="J2N67_006738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133991.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001133991.1"
                     /translation="MPNTIEFKQVEKNARIRDLEIENEKFKQDHHEVSKDELEKAMETM
                     AKATGKELYIGTKKSPHSRVRFAQSLQENLGFLNKNGYLTNKEKVFLSDITPYIAFSSN
                     CIVHDIKAKNPVPANVSEIAKLIGISRQNTSLAINSLVKKGLLFKGDSGVEGNNAKAYA
                     VFVNPHIIYAGDKDSVNEALQVMFYKAMKMKILKDLPDKLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            4747..5838
                     /locus_tag="J2N67_RS33465"
                     /old_locus_tag="J2N67_006739"
     CDS             4747..5838
                     /locus_tag="J2N67_RS33465"
                     /old_locus_tag="J2N67_006739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_042595186.1"
                     /translation="MNVQLENEQITKLLNDWYQSMLKQQLVKAKQLKEYIDSEINNVKE
                     NQNLLLYYSLLDFRYKALTDWISINENSFDEMDNFTTPADDFLAYYYHFFKAFHSTLTS
                     NYTEASEHYEKAKELLIHIPDPIEHAEFNYRMGYFYYQIYKQVIALDYIKLAKEEFSKH
                     EGYEINIALCDNFIGLCCIDLKHFELAEESFNKALDTFQKNNHKNSMLMVRHNLAWLYS
                     KQNLSKLAIRHVSEVTKNNPNHFKAFFVEAFEYYKINDHNYAEQLIEKGLKLSNDFNNL
                     EFQHRFKILKALNNKVPTLTLETSISEGITYFKQEKLWECVKEYADILALRFYEENNHN
                     KASQYFYMSNTAQKNELEKGALK"
                     /name="RapH N-terminal domain-containing protein"
     gene            5838..5996
                     /locus_tag="J2N67_RS33470"
                     /old_locus_tag="J2N67_006740"
     CDS             5838..5996
                     /locus_tag="J2N67_RS33470"
                     /old_locus_tag="J2N67_006740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000758643.1"
                     /translation="MKKVYLTILGISLISILSLGLNDSFKNQAVNIGDGGGAPARPDYF
                     LQTHSNR"
                     /name="hypothetical protein"
     gene            6618..7187
                     /locus_tag="J2N67_RS33475"
                     /old_locus_tag="J2N67_006742"
     CDS             6618..7187
                     /locus_tag="J2N67_RS33475"
                     /old_locus_tag="J2N67_006742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042595188.1"
                     /translation="MKKILFFILTIVMISTFSLPNKYVQAAQISKQPSLDTYLTPEIEK
                     EINNNMQPATKENPISSYTASNGLKVVDTVKTEPAFSDSFSSTSYVTHSKTFYASGLSI
                     ATYKGKWLCTFWRDGSVTIKNWDYFLVGINDGSYSNKSTSILSSGKPAKARGYVTLQSG
                     FFTGDTIWNISISNGSSVSSSITKTR"
                     /name="hypothetical protein"
     Typing          complement(1108..2420)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.36"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagtataaaa tcccgtttaa tgccttcctt ctgtttgccc ccacttcgca aacagactgg
       61 ctagtccgaa taggctaaca caggcgcctt tccttactgc gaagactaag gggcaaaggt
      121 ctggtgtgga aatcctttta ttcttgtgtt ctgaatgtat actttcctta cggaaacacc
      181 tatttttcta aaaacctctt aaaacccaat aaaatcaata atttgaaagt aaacttctat
      241 tcaaaagttt actttcaaaa agataaaaaa gagaaccaac tctgtataac agttggttct
      301 cttttttatc tttcgttcct actagggtaa ggatacacca atttatttcg tcccaacttt
      361 atggcatggg tactccgaaa aaaacagctt ctatctttac aattattgta gcatatttta
      421 atttttttgt gcattttaaa cagccactaa acgaccactg agggccactg gaagaccaca
      481 gaacggctat aaacgaccac cagacgacca cagatcatag aaaaagcgta ccagctagaa
      541 agctttagaa acctaatagg cacagcctat tttcccactt tttatacaaa gtgggattcg
      601 gtttccttat gctcttttta atacttatat tttaatctcc aaaaaaaagt gcaaaatcta
      661 tcaatagatt ccgcaccaac aacagataaa aaatacagtc actagaaaca taggagagtg
      721 tttcaccact tattataaac catatcttta catttttgtg ttcggatttg aaaatatgta
      781 aagatccatc cttttatttt tgaattttct ttcaattcgt agtatactaa atttatacaa
      841 actattaatc tccttacgcg ttatctgata gtgcacaaca aaaaaaccaa ttcccgtgta
      901 atagaattgg tttttttgtg caattcagtc aatacagcaa cttgaaccca cattttcaaa
      961 tgtagtctta tcaatcttaa cataatttta attatataaa ctcaaaaaat ctctttatat
     1021 gcacacatat aaagagattt ttaaatggat gaaacccata aattagttag tgatgaataa
     1081 ttatagtata tcacaaaacg tttctaacgt tccatgtcat atcctttctg tctacttctt
     1141 atttctcttg tatgttcacg ttcaaattga ttatctacac ccttcatatc cagttcattt
     1201 ttaataagtc ctctaaacgc cttaaattgc tctttaaaga cttttttcgt ctgttgatat
     1261 aaaaccttta tattaatctg caagtctctt atatgagcct ttaaagagct tatttctgta
     1321 cttaattgtt tgttttcctt ttgtaacttt aaatttctct tttgagaatc ttgaagtgaa
     1381 tcgcatattt catcgactgc ctcatgtaat tgtttgtttt cttttacaag atccgttttt
     1441 tgcaaacgtt cataatcgct ctttataata accgctgcat tcactttttc tgtcatatct
     1501 ctccattgct cacgtgtaac cacaatgttt cctgttttct tctcagtaat ttctggcttt
     1561 ccaaacagtt tcgttgttac ttctgttttt gtttcttctc gcaaataaga cattgacact
     1621 ggcttatccg gaatttgatt tcgaaacgtt tcaaattcct gtttcttttc ttttattttt
     1681 ttatctaaac tcaaaaattg ttgctgaatt ttttgtaacg tttcattctg tttttcgatg
     1741 tagtttgtgg tttgttgtaa ttgattatga gtttccacta cttttgtctt taaatgcgtt
     1801 tttacattgg ataactgttc cacttctact tctaaatctt gtttttcctt aaaggcttga
     1861 ggcgataaat gttttttctc agaaccttct ccacgctcta catcaaaacc tttttcccgc
     1921 atttccaacg gaaagtcctg ttgcaaagat atcaattctt ttctcgtaaa catatctttt
     1981 gcggacaaac gattgtcttc tgtaattggc atgacaccaa tatgcatatg tggtgtcgct
     2041 tcatccatat gaaccatcgc atacaatgtg ttttgtttgc cataacgctt ttgaataaaa
     2101 tctagtgaac tctcaaaata acgtttctgt tcttctaaac ttaatgaatg catgtaatca
     2161 ggcgaagctg ttactacaaa ttcagaaagt acaacggaat ttgctcgtgg ctttcgtttt
     2221 actcgttcac tgatctcttg tttaattgtg ctttcatacc taattttttg ctgattcaac
     2281 aaatcataat tttgttcact tttcgaataa tcaatatcca aattcgattt actttttcct
     2341 tgtctttggt tgtgtttttg aatcccttgc acatcagaaa tttgaaattt ctgcatgtga
     2401 atgattgcga atttgttcat gtttttccct cacaattgtc ttcggtaaga ggactacttt
     2461 tgaaactatc ataacatatc gagagagcga atccaaagtt ttggtatagc ccactatacc
     2521 aaattacatt tggtgtgggc tctgcgagac cttgttggct tcgccaaatc acgccatggc
     2581 gtgataaaat ccctggggct ttgccccaca ccccacaagg ggaactcttc cccttgaccc
     2641 catcaatcga actccccaac ccctcaatcc ttgaggggct tcctcgccgg ttggcgtccc
     2701 caaaacgatt cgttttgagg aactgccaag aggatggaga atcggctgca cgtatcaatt
     2761 ccttaatgct cctttcatgg atcagcacaa accgctgacc cattccagtc cgcttaccat
     2821 tgacacttac tgcttatttt tcttctccca atagtcgttt tgcgagttgt tccaatcgct
     2881 cgttaaatcc tatgtcgtag gctatgtagc catttcctcg tcttctctcc aaaatttctt
     2941 ttaatacagc cttatcttct tcttgtaatt ctacatcgcc taaaatgatc tctttcggct
     3001 gtctaatttg cacacctaaa gctgttaact ttgcataatt aggaactgtc ataaatcgca
     3061 tttcagacaa aagttttaaa cgctccattt ccttttccgc aacatagaat ttaacctgtt
     3121 gtttagacat ttacacgcct cctatcaaac taaatagtac tattttaata gttttattat
     3181 agaccttttt agtactaaat tgaatcatga atgtatcttc cccctgtttt tgggtgtgag
     3241 tgctggctag tcgtgtgggg gctaaacccc acatagcgtt gatacatatg gaaaaagaat
     3301 catgctttga acggaagaat tggcggttcg gctggtacaa ttgctagtcc aagtcgagca
     3361 acttaacccc gtttttggtc tgttcggctt ggcgaaagct aaaaaccagc cgaaaaaagt
     3421 ttggcaagga tgaaacgtag tgaaaaaggt ggagattttt gagtgctttt tactcaaaaa
     3481 tacgacctgt tccttgcgga acttttagcc ctctatatat agagtgagaa gagagagagt
     3541 tttaaaagtg aaaaaaacat tgatatatca gtatttacaa agcttttcgt gtgtcacgaa
     3601 atcatgacac atgtgtcacg aaatcatgac acacgaaaag ctaaaaaaat aggattgacg
     3661 aattgtcaat cctaaaataa tttatctggc aaatctttta atattttcat tttcatagcc
     3721 ttataaaaca ttacttgaag tgcttcattc acactatctt tatctccggc gtaaataatg
     3781 tgtggattta caaagaccgc atatgcctta gcattatttc cctcaacacc tgaatcccct
     3841 ttaaataaaa gacccttttt aactaatgaa tttattgcta agcttgtatt ttgtcttgaa
     3901 atgccaatta attttgcaat ctctgaaaca ttagcaggaa cagggttttt agctttaata
     3961 tcatgaacaa tacaattgct cgagaaagct atataaggtg ttatatcact taaaaatact
     4021 ttttctttat tagttaaata tccattttta tttaagaaac ctagattttc ttgcaatgat
     4081 tgagcaaacc tcactctcga atgtgggctt tttttagtac ctatgtaaag ctctttacca
     4141 gttgcttttg ccatagtttc cattgctttt tctaattcat ctttggaaac ttcatgatga
     4201 tcttgcttaa atttttcatt ttctatttct aaatcacgta ttcttgcatt tttctcaact
     4261 tgtttaaatt caatagtgtt tggcatattt ttctcctcat tactagtatt tacaactagc
     4321 gaagagactt ttattttgtt attatgtagt tagataaatt gatttgagtc tgaacactct
     4381 aattaattgt ataacagtaa gaaaagccct tctcctatag ttgtagctta gacaactcaa
     4441 ttatagcatg aatggctttt tttgttatat cttgtctgac tttttatatt ccaaaaaaca
     4501 aatatataca gttttaacta aatttgatat cctaaaaatg tcaataagac taatatctta
     4561 tttacgccca ttagcaaaaa gcccttgata taaaagggct ttttgtcatc catataaaaa
     4621 caaagataaa ataaacagaa tattaaaaat aatatatatc aacttattta aaagttaatc
     4681 taatgtacta ccatgataac atggtaaaaa caagatatag ataattttgg aaaaggggaa
     4741 gattctatga atgttcagtt agaaaatgaa caaataacaa aattattaaa tgactggtat
     4801 caatccatgc taaaacaaca attagttaaa gcaaaacaat taaaagaata tatagacagt
     4861 gaaattaaca atgtcaaaga aaatcaaaac ttactcttat actattcttt attggatttt
     4921 agatacaaag cattaacgga ttggataagt attaacgaaa acagttttga tgaaatggat
     4981 aatttcacaa ctccagcaga tgacttccta gcttattatt atcacttctt taaagcattc
     5041 cattctactt taacatccaa ttacactgaa gcaagtgaac actatgaaaa agcaaaagaa
     5101 ctattaatac acattcctga tccaatagaa catgctgaat ttaattatcg aatgggatat
     5161 ttttattatc aaatctataa acaagtaata gctcttgatt atataaaatt agctaaagaa
     5221 gaattttcaa aacatgaagg ttatgaaata aatatagcac tatgtgacaa ctttataggc
     5281 ctatgttgta ttgacttaaa acatttcgaa ctagctgagg aatcatttaa taaagcccta
     5341 gacacattcc aaaaaaataa ccataaaaac tctatgctta tggtaagaca taatttagcc
     5401 tggttatatt caaaacaaaa tttatctaaa ctggcaattc gtcatgtatc tgaagtaaca
     5461 aaaaacaatc caaatcattt caaagcattt ttcgtagaag catttgaata ctataaaata
     5521 aatgaccata attacgctga acaactaatt gaaaaaggct taaaacttag taacgacttt
     5581 aacaatctag aattccaaca tcgttttaaa atcttaaaag cactcaataa taaagttcct
     5641 acattaactt tagaaacatc aatttcagaa ggtattactt actttaaaca agaaaaacta
     5701 tgggaatgtg taaaagaata tgctgatatc ttagctctaa gattttatga agaaaacaac
     5761 cataacaaag caagtcaata tttttatatg agcaatacag ctcaaaaaaa cgaattagaa
     5821 aaaggagcgt taaaataatg aaaaaagttt atctaactat acttggaata tctttaatta
     5881 gtatcctatc gttaggatta aacgactctt ttaaaaatca agctgtaaat ataggagacg
     5941 gtggtggtgc acccgcacga cctgattatt ttttacaaac acattctaac cgataaaaat
     6001 aaagtataag aaaacacctt caaaaaatga aggtgttttc ttatacttta ttttttataa
     6061 atgaaaaact gaatatacca aagttcacta aaccaccatt atattaaaat taataacaaa
     6121 atattaaata aattcaattt catgtttata attatattaa ttccacaaaa tatccaaaca
     6181 acaaaaaaca gaattagtta aaaaaactct ataaatagtt ccctttcctt tttacaaatt
     6241 acaaaaaaag gccaattaag gccctttaaa taatctttta aatctattaa ttagttatat
     6301 ttcttttaaa acgtatttta agaactataa catatatacc aattgcaatt aaagtacacc
     6361 aaatcaatga aatcatgtta aaaaacaaca tcaatattac atttgtcaat attacagata
     6421 aaattccaaa aacattttta ttactagaaa ttaaattata aacaactaaa ccaattatcg
     6481 ttgaaataat cgtacaaaaa aggatcaata taaaatacat acaaataaac ctccttttta
     6541 catttctgtc tttattttct aaatattttg tattatcata ataaatgtac caaataataa
     6601 tatcaaggag gacaattatg aaaaaaattt tatttttcat cttaacaatt gtaatgatta
     6661 gtacattttc attaccaaac aaatatgtgc aagctgccca aatttccaaa caacctagct
     6721 tagatacata tttaactcct gaaattgaaa aagaaattaa caataacatg caacctgcaa
     6781 caaaagaaaa tcctatttca tcttatacag cttctaacgg cctaaaagtt gtagatactg
     6841 tgaaaacaga acctgctttc tctgattctt tttcttcaac atcatatgtt actcattcaa
     6901 aaactttcta tgctagtgga ctttccattg ctacttataa aggaaaatgg ttatgtactt
     6961 tctggcgaga tggtagcgta acaattaaaa actgggatta tttcttagta ggaataaacg
     7021 atggttccta tagtaataaa tctactagta tactatctag tggtaaaccg gcaaaagctc
     7081 gtggatatgt aacacttcaa agtggattct tcacaggtga tacaatctgg aatataagta
     7141 tttctaatgg ttcttctgta tctagttcaa ttacaaaaac aagataacaa aagccacatt
     7201 aatgtggctt tttatttttc aataaataat gttttttacc a
//
