LOCUS       NZ_CP072702             8553 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain IMBL-B9 plasmid pKBT8553, complete
            sequence.
ACCESSION   NZ_CP072702
VERSION     NZ_CP072702.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18221107
            Assembly: GCF_023921665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Park,M.G., Choi,J.Y., Kim,J.H., Park,D.H., Wang,M., Kim,H.J.,
            Kim,S.H., Lee,H.Y. and Je,Y.H.
  TITLE     Isolation and molecular characterization of Bacillus thuringiensis
            subsp. kurstaki toxic to lepidopteran pests Spodoptera spp. and
            Plutella xylostella
  JOURNAL   Pest Manag Sci 78 (7), 2976-2984 (2022)
   PUBMED   35419912
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Park,M.G., Choi,J.Y. and Je,Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) Department of Agricultural Biotechnology,
            Seoul National University, 1 Gwanak-ro, Seoul 08826, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023921665.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 14:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,736
            CDSs (total)                      :: 6,585
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 118 of 328
            Pseudo Genes (incomplete)         :: 239 of 328
            Pseudo Genes (internal stop)      :: 81 of 328
            Pseudo Genes (multiple problems)  :: 86 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072702.1.
            Bacteria and source DNA available from Laboratory of Insect
            Microbiology and Biotechnology, Seoul National University, Republic
            of Korea.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="IMBL-B9"
                     /db_xref="taxon:1428"
                     /plasmid="pKBT8553"
                     /geo_loc_name="South Korea: Suwon"
                     /collection_date="2018-04-13"
     gene            complement(3..356)
                     /locus_tag="J2N67_RS33360"
                     /old_locus_tag="J2N67_006719"
     CDS             complement(3..356)
                     /locus_tag="J2N67_RS33360"
                     /old_locus_tag="J2N67_006719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252598148.1"
                     /translation="MIDSSTLSRIVSYSVGKFVLVCCADSSVHLGLLVPSDLAKKTSFF
                     EVDNDLGFSFDDLGVYSDQMLFIFTLESELLSIPYDQVDRVITFTGSCIDSHNKFYVEP
                     NEFLNEFKLYMSV"
                     /name="hypothetical protein"
     gene            complement(746..898)
                     /locus_tag="J2N67_RS33365"
     CDS             complement(746..898)
                     /locus_tag="J2N67_RS33365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252598150.1"
                     /translation="MRNIKEFKEIARKNLDKYVVVSTSENDTFEGVLLSVFFSKEVVKI
                     AEDIF"
                     /name="hypothetical protein"
     gene            complement(930..1172)
                     /locus_tag="J2N67_RS33370"
                     /old_locus_tag="J2N67_006720"
     CDS             complement(930..1172)
                     /locus_tag="J2N67_RS33370"
                     /old_locus_tag="J2N67_006720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391897.1"
                     /translation="MEELLTKILEKLDDVDKKVDKVSGDVQELKQELKAHRMEIDDNFT
                     KLYKLVEEKDVEIDVLNKRVFKNETTVEKLKRQKH"
                     /name="hypothetical protein"
     gene            complement(1186..1491)
                     /locus_tag="J2N67_RS33375"
                     /old_locus_tag="J2N67_006721"
     CDS             complement(1186..1491)
                     /locus_tag="J2N67_RS33375"
                     /old_locus_tag="J2N67_006721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_014893149.1"
                     /translation="MENETIQEKNKRRRVPVRFSEITAQKLDDMSDRYGMSVSALVNFI
                     VGQWLDNNYDIKDYTREKMIPAMMENVEPQMESMLQNPLIAGMFKEMAEQFVKASK"
                     /name="hypothetical protein"
     gene            complement(1760..2281)
                     /locus_tag="J2N67_RS33380"
                     /old_locus_tag="J2N67_006722"
     CDS             complement(1760..2281)
                     /locus_tag="J2N67_RS33380"
                     /old_locus_tag="J2N67_006722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_252598152.1"
                     /translation="MVVIYQTTKQAAQTLNINHTTFKKYYGMFERYNGYNFLRDSKGQV
                     MFSEYDLEVFKRLLSVKAEPGRTIEESCRVVGEEFGISNKNQVITDISPENGEDNKAIE
                     ELKELILMQNNKIDELTIKLNEQSNQTKMIETSVGDRDQQLVRLMKEMLEVKRMVAASN
                     KKRWWHFFKR"
                     /name="DUF3967 domain-containing protein"
     gene            complement(4430..4681)
                     /locus_tag="J2N67_RS33385"
                     /old_locus_tag="J2N67_006723"
     CDS             complement(4430..4681)
                     /locus_tag="J2N67_RS33385"
                     /old_locus_tag="J2N67_006723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000888627.1"
                     /translation="MLEDKFLESKIKKLTNQNVVVFTDDEIIEGVLLDKDTGEERLFEV
                     FRKIWGKNFAWFGEGIYIQCEDTVTFRDYDEVDNIFKK"
                     /name="hypothetical protein"
     gene            4793..4933
                     /locus_tag="J2N67_RS33390"
                     /old_locus_tag="J2N67_006724"
     CDS             4793..4933
                     /locus_tag="J2N67_RS33390"
                     /old_locus_tag="J2N67_006724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000130115.1"
                     /translation="MTDKKERVEMRIPQSILKKVDEYKEENGISTRTATILELIRKGLN
                     K"
                     /name="hypothetical protein"
     gene            complement(4965..6011)
                     /locus_tag="J2N67_RS33395"
                     /old_locus_tag="J2N67_006725"
     CDS             complement(4965..6011)
                     /locus_tag="J2N67_RS33395"
                     /old_locus_tag="J2N67_006725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3895 domain-containing protein"
                     /protein_id="WP_252598145.1"
                     /translation="MYGAYLDLEDRDRIMGSLSDEQQEYLRNFLKRGNQTVFSSMLARL
                     KGGESEEDAEHISTQWMFIDYIDAGKVSKDHPCECGRPLRYQYIVKNLVTNKILKFSKD
                     HFEKHTGLPSNIVKAVLQGMYEIDRELDEILIKTSSGWSLFENLGVDSIPNDLIIPIDI
                     KEHLKLDLPLLDRQVKRLKNNFKDYNIARKNLVKKDDFEDFKTKDKKDKKDKKDKKEEE
                     QVSFFDEDIIFGVNSDEDNIQVVQNQPIKVKPVSVEKNILYQFLSFVEQEFILQFIQGV
                     DSISGRQLCEFMIKEVGSLDKRYLTGKPHIYVYVCSYLDFLVTQGKLSYIEDVGYMDRI
                     YYREFGVI"
                     /name="DUF3895 domain-containing protein"
     gene            complement(6150..8528)
                     /locus_tag="J2N67_RS33400"
                     /old_locus_tag="J2N67_006726"
     CDS             complement(6150..8528)
                     /locus_tag="J2N67_RS33400"
                     /old_locus_tag="J2N67_006726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_252598147.1"
                     /translation="MALDVSKCGKKTAHLKSKETGKHYAKALTCNKLECPRCQPVIAAH
                     VQKMVRFYSQRERLFFFNTLTSTDGIKDLDKIFSKIRNELNFNFTLEGYMKRKKVSYEK
                     AVKWYEKKKEKTILSDVEVELLRISRYDAVISVAKRKKVYYKNLSSDDKAKFKDIYSDL
                     IYERFLYIYDCKCKDQSLRDELYNNISKRFEENEFVDFKFIRVLEFHKSGQPHYHFLSN
                     RYVPHTLLKKLTVEGVSEVYDNTYIVEDALRKNPDLTYDSVDTDIVSNYVSKITNYVTK
                     DTIDTFLTMQSDGDISKKLISSSNSIKIFKEKDEKDLKYIKLGVFDYQLNSSSYEIPSN
                     VGDVYESVEGFIKEHSKMLRDEHAIRLHHLVRELDLSDEIKYSYMIHERLLQISSMDFL
                     VERLGDYRTEGLTFDQVRLMRSFIDNRITLLLGRAGTGKTYTLVNLLNRLKPDPNKTIV
                     CTYTGKASSRIRELFREYDLQDYKSLTIHKTCSSNFNSKFFKNEYNIIDCAYLIIDEVS
                     MIPREILAKLLQAVPSHVKIIFAGDDAQLPPVNDTSIIPELEALDFVNTVRLTQVFRSE
                     DAVLGQAYNVLNRKSIDYNLFGEDMKELVLKLISDGYQILTNTRKLAKDINMIVQAEKK
                     DITLCFDDFKYDLNDRVMIIANSSARNVSNGDTGKIVDYDECGVCVCLDLEDREVFYKY
                     EDTEEIVPAYAFTVHKSQGSEYENVAVFVDSQPKLNTNNLLYTGITRAKKNIKVYVPDE
                     MVLSTMIKRVPEPVSNITLNNVFDLVNGILDIKKTSSMG"
                     /name="AAA family ATPase"
     Typing          complement(6151..8528)
                     /name="rep_cluster_2109"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatcaaacag acatatacaa tttaaattca tttaaaaact cattaggttc tacataaaat
       61 ttattatgac tatctataca agaaccagta aaagttataa ctctatcaac ttgatcataa
      121 ggaatagata acaattcaga ttcaagagtg aaaataaata acatctgatc actataaaca
      181 cctaaatcat cgaatgaaaa tcccaagtca ttatcaactt caaaaaaaga agttttctta
      241 gctaaatcag aaggaactaa aagaccaaga tggacagaag aatcagcaca gcaaacgaga
      301 acaaacttac ctacactgta cgaaacaata cgagacagag tagaagaatc gatcataaaa
      361 ccacctccgt aaaagcaaga aataaaaaag tgagcgttta tcccctatta atattaacaa
      421 gttaagtaaa gaactactca aaccccaaaa aacatttttt gattcaccaa taataattct
      481 ataatgatcg ctcaacaaag cgcccattta cacagtgaac tgtcaaacag gttttattac
      541 ataaactcaa cgtttactaa agtaaatatt aagaatgtgc atgcaaccat tctttaaaat
      601 actcaaacat ttttttagaa tcctcttcaa atatagaaca atcataaact ctaataactt
      661 caataccttc ataaaacaca aaattaactc cagtaccatc aaatagaaaa atccctttta
      721 aaatcaagtt atcagaatta aaatcttaga aaatatcttc tgcaatcttc acaacttcct
      781 tgctaaaaaa aacacttaat aaaacacctt caaaagtatc attctcactt gtcgaaacaa
      841 ctacatattt gtcaagattc ttcctagcta tttccttaaa ctccttaatg tttcgcaaga
      901 tagcattcgc cttaaatctc tttttaaaat taatgttttt gtctctttaa cttttcaact
      961 gtcgtctcat ttttaaatac acgtttattt aaaacatcaa tctccacatc tttttcttca
     1021 actaacttat ataatttcgt aaaattatca tctatttcca tacgatgtgc ttttaattct
     1081 tgttttaatt cctgcacatc accacttact ttatctacct ttttatcaac atcatctaat
     1141 ttttctagaa ttttcgttaa taattcttcc atacaaatcc ctcctttatt tagaagcctt
     1201 aacaaattgt tcagccattt ccttgaacat tccagctatc aatggatttt gaagcataga
     1261 ttccatttga ggttctacat tttccatcat tgccggtatc attttttctc tagtgtaatc
     1321 tttaatatca taattattgt ctaaccactg accaacaata aaattcacca aagcactcac
     1381 tgacattccg tagcgatcag acatatcatc taacttttgt gccgtaattt cagaaaacct
     1441 aacagggaca cgtctcctct tattcttttc ttgaattgtt tcattttcca taaaaaaagc
     1501 ctccttatta caaaaactaa tactaaaatc cacaaatcta atatgtaaaa gtgcaattct
     1561 catatgcaaa aacataattt acatattaaa aaagagattt tgtatatacg gtgctattgt
     1621 attgtatata caatacaata atgaatccaa aatgacacca ctattttttc tttctgttac
     1681 taatattatc ttaaaaatag catataaaca agaaatattt aacaaaaaaa caaataaaaa
     1741 aaagccttat cggctaaatt tatcttttaa aaaaatgcca ccatcttttt ttattacttg
     1801 ctgccaccat tcttttcact tctaacattt ctttcattaa tctaactaat tgctgatctc
     1861 tatctccaac gcttgtttca atcatcttag tttgatttga ttgttcattt aatttaatag
     1921 ttaattcatc gattttatta ttctgcatta aaatcaattc ctttaactct tcaatcgcct
     1981 tattatcctc tccattttca ggagatatat cggttataac ttgattctta ttcgaaatac
     2041 caaactcttc accaacaact ctacaagatt cttctatagt ccttcctggt tccgccttaa
     2101 ctgataataa cctcttaaat acttctaaat catattcact aaacattacc tgtccttttg
     2161 aatctcttaa gaagttatat ccgttatatc tctcaaacat accataatat tttttaaaag
     2221 ttgtatgatt aatatttaat gtttgtgctg cttgttttgt tgtttgatat ataacaacca
     2281 ttgcaatcac ctcacaaatt tacattcgat ataaccgata taactccttg ttttataatt
     2341 tatataaact ttataaatca caataaaatt tcacgtcccg tattttcctc aaaattaaac
     2401 cgtccttttt aaaaaaaggg acacaaaaat tttttatttg tgggatattc ccacaccctt
     2461 tacgctcaca gcgggtgtcc cccaaacaat gtttgggagt acgcaatagc ttggtttact
     2521 attcctttag gcaaccctac gggacatcaa cttttattta aaaaactttt tacaaaccaa
     2581 aaaacttttc taaataaaac tccttcgcct tgctacggct tcttacaggc tttccatttc
     2641 catctaccga ttcacatttc aaacctttca gaagtgattt tgcgtactct gatgaatata
     2701 tacatcgttc cttcttttaa tcattctacc tttatacctg aagttatatc ggctatatct
     2761 tctataactc acaattaaat ccaactatat attaaatacc tctatagata actaccttgt
     2821 taatattttt catcctctta acgcttaact atccctggcg cgcatccgcc taaggccgaa
     2881 tgctcgccat acctaacata acctctaggg cgtaagcact aattctgcga caaatattaa
     2941 aaacattttt tctatagaat ctctaaatat atacttatct cttcttgaga cccaagcact
     3001 atatccgtat ttaaacctcc aactaccctg aatttagtcc tgctgccatt tcattttgat
     3061 ttttttataa caattacttg tataaaaatc ttcatgcttc actccaatat acttcaaaaa
     3121 ctgtcttccc taatcttcgc tattcctcgc taattaccac aaacattaat tatttaatca
     3181 atcggatcta ttagtaatgc tacatcaaaa cattagtaac atagacctta taaatatttt
     3241 gtaaccacaa aacatattac ctttttcaat tgtggccaca accctgtaca caatgaaaaa
     3301 ttattctatc aggctttgac tgatacacgt agacacaact gtatccctct ccttatcctg
     3361 cattttcttt ttttaataca aaatatttac cagcttaaaa agccatataa accttttttt
     3421 aaagaagagc ataaggaaac cgaatcccac tttataaaaa aagtggtgaa ataggctgag
     3481 cctattgggt ttctagtttg tgaattgcgg gatgcctttt tgggctttgt agcccgtttt
     3541 gtgtacgttt aaagcacttt tgtggcacgt tttgtgtccc ttgtagcacg ctttacgccc
     3601 ctttaaatca acgttttttt aaccccaaaa agtgggacgt ctttttttta ctcatatgcg
     3661 ttctgtatgc gtttttggtc attttgtatg cgttttatat gtgaaaaaaa ttaaaagtat
     3721 gcgttttgta tgcgccaaaa atgaccacta aacgaccacg aaaactggga cgcctattta
     3781 ttaattggtg gtcactgggt ggtcattttt tcattctgat ggtcatttgt tggcacttag
     3841 tggtcactca gtggtcacta aaaacgcacc aaaaaattga atttgtggta aaataaaaac
     3901 gaaattcagc ggagttctta ctcattcacc ttcgcaacaa cataaaacaa aagaatgatg
     3961 attaattttg gagtatccat accttagttg gaactaaaaa attggtgtag ccttaccgta
     4021 gtaggcataa aaataaaaaa acgatatgtt aaagctatcc ttttgggtaa cgttaacata
     4081 tcgttttttt attcgctaat acaataatgc acatggctgc tgtgcattga tcctcatcaa
     4141 cctcttctta aaagctccta taaaactatt aaaatataca acatttataa atggaatttc
     4201 attttatctt ttacaagcat tatcaatcta aaagcaaaaa aaccaatttc caatagcata
     4261 ttatatacag tgaaaaccca ttggaaacac attgaaactc cattgatttt ctcatattta
     4321 tgatatcacg tttttgttag gctttgtcta acctgtcacc tttagtgata gcaaaaaaac
     4381 cttatgctct tctataaata atctgcattt aatataaatt tatatagaac tatttcttaa
     4441 atatattatc tacctcatca taatcacgaa acgttactgt atcttcacat tgaatataaa
     4501 ttccttctcc aaaccaagca aaattcttac cccaaatctt acgaaaaact tcaaacaaac
     4561 gttcttctcc agtatcctta tccaaaagga ctccttcaat aatctcatca tcagtaaaaa
     4621 ccactacatt ttgattagtt aattttttaa tcttagattc taaaaatttg tcttctaaca
     4681 taataaaatc ccctttgatt ttcgtttttt tgtacggaaa aaatacgtct tttgtccgta
     4741 caatttgtat tatacgatat ttttccgtac aatacaagaa gaggtgataa taatgacaga
     4801 taaaaaagaa agagtagaaa tgagaatccc acaatcaata ttaaaaaagg ttgacgaata
     4861 taaagaagaa aacggaattt ctactagaac agcaacgatt ctagaactta taagaaaagg
     4921 gttaaataaa taaaaaaaaa gaagggataa tatacatatc ccttctaaat aacaccaaat
     4981 tctctataat aaatccgatc catataaccc acatcttcaa tataacttaa cttaccctga
     5041 gtaactaaaa aatctaaata actacaaaca taaacataaa tatgaggttt tccagtaaga
     5101 taacgtttat ctaaagaacc aacttcttta atcataaatt cacataactg tcttccacta
     5161 atagaatcaa caccttgtat aaactgtaag ataaattctt gttcaacaaa acttaaaaat
     5221 tgatacaaaa tatttttttc aacagaaaca ggttttactt ttataggttg attctgcact
     5281 acctgaatat tatcctcatc tgaattaact ccaaaaataa tatcttcatc aaaaaaagaa
     5341 acttgttctt cttctttttt atctttttta tcttttttat cttttttatc ttttgtttta
     5401 aaatcttcaa aatcatcttt cttaacaaga ttctttctcg ctatattgta atctttaaaa
     5461 ttatttttca atctcttaac ttgacgatct aacaaaggaa gatctaattt caaatgttct
     5521 ttaatatcta tagggataat taaatcatta ggaatagaat ccacacctaa gttctcaaat
     5581 aaagaccaac cagatgaagt tttaatcaaa atttcatcca actcacgatc aatctcatac
     5641 attccctgca aaaccgcttt aacaatatta gagggtaaac ctgtatgttt ttcaaaatga
     5701 tccttactaa actttaaaat tttattagtc actaaattct taacaatgta ttgatagcgt
     5761 aaaggacgac cacactcaca cggatgatct ttcgaaactt ttcctgcatc aatataatca
     5821 ataaacatcc attgagtaga tatatgttca gcatcctctt ccgattcgcc accttttaat
     5881 ctagctaaca tactagagaa tactgtctgg ttacctctct tcaaaaaatt acgtaaatac
     5941 tcttgttgtt catcagacaa actacccatt attctatccc tatcttctag gtccaaataa
     6001 gcaccataca taaacaacaa atccttccga aaaattaaat taaatagacc atgctacaaa
     6061 ttacatatat aatacaatta gattctttaa caatcatttc agtcttatga ttcttcaacc
     6121 cattgaagaa tctacatttt tatacatctt catcccattg aagatgtttt ttttatatct
     6181 aaaatcccat ttactaaatc aaaaacatta tttaatgtta tattcgaaac tggttctgga
     6241 acacgtttta tcatagtcga taaaaccatt tcatccggca cataaacttt tatatttttc
     6301 ttcgctcttg taattcctgt atataataaa ttattagtat tcaatttcgg ttgactatct
     6361 acaaataccg ccacattctc atattcacta ccttgagatt tatgaactgt aaatgcataa
     6421 gctggtacaa tttcttcagt atcttcatac ttgtaaaaca cttctctatc ctctagatcc
     6481 aaacaaacac aaacaccaca ttcatcgtaa tcaacaattt ttccagtatc accattacta
     6541 acatttctag ccgaactatt agcaataatc atgactctat catttaaatc atacttaaaa
     6601 tcatcaaaac aaagtgtaat atctttcttt tcagcttgaa caatcatatt tatatcctta
     6661 gctaatttcc ttgtgtttgt aagaatttga tatccatccg aaattagttt taaaaccaat
     6721 tctttcatat cctcaccaaa caaattataa tcaatacttt tacgattcaa aacattataa
     6781 gcttgtccaa gtacagcatc ttcacttctg aaaacttgtg ttaatcgaac agtattaaca
     6841 aaatctaacg cttctaactc aggaataata cttgtatcat ttacaggtgg caattgagca
     6901 tcatcacccg caaaaatgat tttcacatga ctaggaactg cttgaagtaa tttcgccaaa
     6961 atctcacgtg gaatcattga aacttcatca ataatcagat atgcacaatc aattatgtta
     7021 tattcatttt tgaaaaactt cgaattaaaa ttagaagaac atgttttatg tatagttaat
     7081 gatttataat cctgtaaatc atattcccta aataattcac gaatcctact agaagcctta
     7141 cctgtataag tacaaacaat tgtcttattt ggatctggtt ttaaacgatt taacaaatta
     7201 acaagagtat atgtttttcc tgttcctgct cgtcctaaaa gcaaagtaat acgattatca
     7261 ataaatgacc tcatcaatct cacttgatca aaagtcaggc cctctgttct ataatcacca
     7321 agtctttcta ctaagaaatc catagaagat atttgtaaca aacgttcatg aatcatataa
     7381 ctatatttaa tttcatcaga caaatctaat tctctaacta aatgatgtaa ccttatagca
     7441 tgttcatctc gaagcatttt tgaatgctct tttataaaac cttctactga ttcataaaca
     7501 tccccaacat ttgaaggaat ttcataacta ctagaattca attgataatc aaaaacaccc
     7561 aatttaatat attttaaatc tttttcatct ttttctttga aaatcttaat agaattacta
     7621 gaactaataa gttttttaga aatatcacca tcactttgca tagttagaaa agtatctatt
     7681 gtatcctttg taacataatt ggtgattttc gaaacataat tagaaacaat atcagtatca
     7741 acagaatcat aagttaaatc aggatttttt ctcaaagcat cttctacaat ataagtgtta
     7801 tcataaactt cactcacacc ttctacggta agcttcttta ataaagtatg aggtacataa
     7861 cgattactca aaaaatgata atgaggttgt ccactcttat gaaactctaa aactctaata
     7921 aatttaaaat caacaaattc attttcttca aaacgcttag aaatattatt atataattca
     7981 tctcttaaag actggtcttt acacttacaa tcataaatat aaagaaacct ttcataaatc
     8041 aaatcactat atatatcctt aaacttagct ttatcatcag aacttaaatt cttataataa
     8101 acttttttac gcttagcaac agaaataaca gcatcataac gagaaattct aagcaattct
     8161 acttccacat cagaaagaat tgttttttct ttcttcttct cataccattt cacagctttc
     8221 tcataactaa ctttcttgcg tttcatatat ccttccaaag taaaattgaa attcaactca
     8281 tttctaattt tactaaaaat cttatccaaa tccttaatac catcagtcga agtaagagta
     8341 ttaaagaaaa acaagcgttc acgttgagaa taaaaacgaa ccatcttctg tacatgtgcg
     8401 gcaataactg gctgacatct aggacattct aatttattac aagtcaaagc cttagcataa
     8461 tgcttaccag tttctttaga ctttagatgt gctgtctttt tgccacactt agaaacgtct
     8521 aaagccataa acaaacacct ttccaagtaa aat
//
