LOCUS       NZ_CP072701            10369 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain IMBL-B9 plasmid pKBT10, complete
            sequence.
ACCESSION   NZ_CP072701
VERSION     NZ_CP072701.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18221107
            Assembly: GCF_023921665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Park,M.G., Choi,J.Y., Kim,J.H., Park,D.H., Wang,M., Kim,H.J.,
            Kim,S.H., Lee,H.Y. and Je,Y.H.
  TITLE     Isolation and molecular characterization of Bacillus thuringiensis
            subsp. kurstaki toxic to lepidopteran pests Spodoptera spp. and
            Plutella xylostella
  JOURNAL   Pest Manag Sci 78 (7), 2976-2984 (2022)
   PUBMED   35419912
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   Park,M.G., Choi,J.Y. and Je,Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) Department of Agricultural Biotechnology,
            Seoul National University, 1 Gwanak-ro, Seoul 08826, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023921665.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 14:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,736
            CDSs (total)                      :: 6,585
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 118 of 328
            Pseudo Genes (incomplete)         :: 239 of 328
            Pseudo Genes (internal stop)      :: 81 of 328
            Pseudo Genes (multiple problems)  :: 86 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072701.1.
            Bacteria and source DNA available from Laboratory of Insect
            Microbiology and Biotechnology, Seoul National University, Republic
            of Korea.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="IMBL-B9"
                     /db_xref="taxon:1428"
                     /plasmid="pKBT10"
                     /geo_loc_name="South Korea: Suwon"
                     /collection_date="2018-04-13"
     gene            complement(342..497)
                     /locus_tag="J2N67_RS33310"
                     /old_locus_tag="J2N67_006710"
     CDS             complement(342..497)
                     /locus_tag="J2N67_RS33310"
                     /old_locus_tag="J2N67_006710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252598138.1"
                     /translation="MKKIILILTSIVLAAGIIYTVASGKEESNNLVKKDSEQLLVRMGD
                     PGGGMG"
                     /name="hypothetical protein"
     gene            complement(509..1717)
                     /locus_tag="J2N67_RS33315"
                     /old_locus_tag="J2N67_006711"
     CDS             complement(509..1717)
                     /locus_tag="J2N67_RS33315"
                     /old_locus_tag="J2N67_006711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016112297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AimR family lysis-lysogeny pheromone receptor"
                     /protein_id="WP_252598139.1"
                     /translation="MDQKVKKEDVPKKNTLVGFLKVLVDDIDFQKKSQEDLAKEIGISG
                     GTFSKNLTGKSQFSFWNLVKLLNILYENDINKKRQMLFTFCSITTSKKNVRIAMEYANA
                     KGDLQLLKSLVDSEKNSSLAMNREWAYVYELVWLRSSGSIKKQELLAKIEDRKGSKVIK
                     TKEMKVLYGILTYYTMYDLKKYNSLFEYAGVLVPEVEEITDNFIKSSYLGRIKEGLAYA
                     YLVQDNLEKSRSLCHEILDIENIEGCFHLLRASAFVYLAESYTFECYDSASRYIKQSLK
                     QLEPYYFEREKYRRQRILNTYAFIKLVNKKNLENIKIYHFAEKSLLEIVKGNYINAVNI
                     LNDLEKENGYLTPMQYCYLGIAKDDISLIEKSIMLFECEGDRFYSKFPKKIMAEFNKNG
                     MIC"
                     /name="AimR family lysis-lysogeny pheromone receptor"
     gene            complement(2424..3083)
                     /locus_tag="J2N67_RS33320"
                     /old_locus_tag="J2N67_006712"
     CDS             complement(2424..3083)
                     /locus_tag="J2N67_RS33320"
                     /old_locus_tag="J2N67_006712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_252598141.1"
                     /translation="MNGKVELQLVKAEKKAQHRENKIDNIFEQLQSGGFSEEDRGMLLQ
                     LLSKATGGEEYYIGKKKKPTDRVKFVQLIMDNVNYLTELEYLSSKEEAFLFKLAPYVEF
                     KTNVIIQQIDKDNADTTTPASPTYLAERFKMARKNISLTMNGLLKKGILGVAESGMITE
                     DGRACTSRTWFVNPNIMCCSPKDGIDKATQHIFRNSLRNFKVDDSKKKYKLPIYLF"
                     /name="MarR family transcriptional regulator"
     gene            complement(3332..3556)
                     /locus_tag="J2N67_RS33325"
     CDS             complement(3332..3556)
                     /locus_tag="J2N67_RS33325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252598142.1"
                     /translation="MTKQENAILETLNVDSVEVEKPVIKKVLPMVKDDEEIKLINDKPH
                     KKVFKFGKELFLELEWEQVPKYRLAELKL"
                     /name="hypothetical protein"
     gene            complement(3513..4526)
                     /locus_tag="J2N67_RS33330"
                     /old_locus_tag="J2N67_006713"
     CDS             complement(3513..4526)
                     /locus_tag="J2N67_RS33330"
                     /old_locus_tag="J2N67_006713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_252598143.1"
                     /translation="MATTKLGNTKQASRAINYAEKKATVKSGHNCDVDYAKTSFKELRM
                     LYGKNDGVQAHTIIQSFRPGEVTPEQANELGLELARAVAKDHQVAVYTHTDKKHIHNHI
                     VINSVNIETGNKYQSNAEQRHFVKSENDRICLENGLEVTERKADIRYTQAEQGLEEKGK
                     NSWKMELRRAINYIKERTGDFKGFSEALEELGVKVKMRGQTLSYKHPEVSKWVRGKTLG
                     DDFTKEGIESEFSRQIQERNDERKFITTSRIDDGDITEQFERTKRKINRAKQNGNRVNE
                     SINRINDRPTKVDGTNVEQNRSTREKQQQQDKNREEIRRKEQRDKARKRHFRDLER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4508..4891)
                     /gene="mobC"
                     /locus_tag="J2N67_RS33335"
                     /old_locus_tag="J2N67_006714"
     CDS             complement(4508..4891)
                     /gene="mobC"
                     /locus_tag="J2N67_RS33335"
                     /old_locus_tag="J2N67_006714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_103141271.1"
                     /translation="MSERDDNMASENSNKEQKQPIRKEPRQISFRVSESEYLKLQQSAE
                     TLNMSVPTFVKKKAQGSRLVAPKFDKATRQSIARDLSMLGNNMNQIAKYCNSNRHGGVE
                     WEKLNDSVEGMRKELNRLWQQLN"
                     /name="mobC"
     gene            complement(5550..6398)
                     /gene="mobV"
                     /locus_tag="J2N67_RS33340"
                     /old_locus_tag="J2N67_006715"
     CDS             complement(5550..6398)
                     /gene="mobV"
                     /locus_tag="J2N67_RS33340"
                     /old_locus_tag="J2N67_006715"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_252598131.1"
                     /translation="MKYLIASMRKKIKFANMNEIPKECAYDLVNKEPVNHESRIMEIIN
                     EQRDSLLTIRKDAVLVSQWYVSVSNDYFEVIEFDKIKEFFQYVVNFFEGRYGKQNIAYA
                     QVHFDGDTPYMLVGIVPMKDGRLQAKNIFDRKELVYIQNELPWFLQKKGIDLERGTTTI
                     AKDIRTKEEVEEVRQLTREMAQEIEQMKKEKQSLQQEYNFVKNQLEWAKDYLKNETNPG
                     IKYIKKVETDKGVTYQISEVELLRLNSIRKGANVLINTNEALEKENQKLLLEIKKLKEN
                     Q"
                     /name="mobV"
     gene            complement(6415..9444)
                     /locus_tag="J2N67_RS33345"
                     /old_locus_tag="J2N67_006716"
     CDS             complement(6415..9444)
                     /locus_tag="J2N67_RS33345"
                     /old_locus_tag="J2N67_006716"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="WP_252598132.1"
                     /translation="MKNKKKILYSFVVSLIMAIITVPFTVYADWDWSDFFGTKDSDIPK
                     ILEFLHNDWIRYTDFLGAILQSFLGGIVKLLLSFVTLVEGLIPDTFSLFDLLKDAGLNE
                     FYTSIMKGLFYSILVLMIVWIGIRVIIQHNPPKFKSVGVNVVIMIGLLGGLNELMADMQ
                     SMATDFYKGSVEQGKAKEGLAWDIVKDNTADLLYLSQVGFESIKKENSKEPKNNLSKEE
                     FLQADLSDLLTLKVIEDISKEGTGMAAETNYLTYKISHTGKEQTVSKIEKASMFNPFKD
                     SFESGYKRYPMQFFTIFWSLLALGVAYLFTLFVFVITIFELVMKKLVAPFVMVTDIDSG
                     QKTKMMIQDIGQGFLTIAFTGLSLRFFTISVNFVANKDLNPVLYVVVLVCLTIALIKGS
                     NTILKYFGVDVGLKEGMNGFLATVGGLSLAKRAGEGMKNMFSGARESASTQQSRDDTPK
                     RSEMDSEDKVNGLNDKDNKKSGVMPKNFMKNTGAALSYATNRGVPGMMNDAGNMVVATM
                     SSAVGKGKEKASGVTKGVSSGVQNTIDEFKEGQAEGKQKAQENNDRQLLNKLGKDIHNN
                     ANSGEDQNVSPKMGSVSNANKQQMEQGNSQSPGQTSQDEILRSKQMMQVEKQQVEQGNP
                     QASGQTSQDENLRRMQTMNVEKKQVEQGNPQTSGQASQDENLRRMQTMNIEKKQVEQGN
                     PQTSGQASQDENLRRMQTMNIEKKQVEQGNPQTSGQASQDENLRRMQTMNIEKKQVEQG
                     NSQASGQASQDENLRRIQTMNVEKKQVEQGNPTVSGGMARVESQRAVQETKVENKAVQG
                     ANPTVSGGTVRAESQRAVQETKVENKAVQGANPTVSGGTVRAESQRAVQETKVENKVVQ
                     GANPTVSGGTVRAESQRAVQETKVENKAVQGANPTVSGGTVRAESQRAVQETKVENKVV
                     QGANPTVSGGTVRAESQRAVQETKVENKVVQGVNPTVSGGTVRAESQRAVQETKVEKRA
                     SGLRQRGIKRNRTDRLRRNE"
                     /name="pLS20_p028 family conjugation system transmembrane
                     protein"
     gene            complement(9512..9733)
                     /locus_tag="J2N67_RS33350"
                     /old_locus_tag="J2N67_006717"
     CDS             complement(9512..9733)
                     /locus_tag="J2N67_RS33350"
                     /old_locus_tag="J2N67_006717"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252598134.1"
                     /translation="MKKGWLLFFGLMLLLFLFGIPLSEIGALIFLAFVGYILIRMFQIS
                     LGCVVILIGFFIVFVVIAFGVDVFSRFF"
                     /name="hypothetical protein"
     gene            complement(9765..10154)
                     /locus_tag="J2N67_RS33355"
                     /old_locus_tag="J2N67_006718"
     CDS             complement(9765..10154)
                     /locus_tag="J2N67_RS33355"
                     /old_locus_tag="J2N67_006718"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252598136.1"
                     /translation="MNHVIDKSVTQLTSKMNRTFEEMETYDSFKFASDSLRKEYENPKS
                     SFLRVLNRFVLRIEKKVLDYLLKRKVDERIVVEQMVDDFSNYDLEQQLQIIESIDEYEE
                     ECMEFGEYMQPVIEDYEIEPYKACI"
                     /name="hypothetical protein"
ORIGIN
        1 ggacaaaact taaagaattt tatgtcgaaa tttgcttaac gtggtttatt tccaaaatgc
       61 tggcggtacc cctttcaaga gatagctggc tttctcaggg cgaagctatc tctttttata
      121 tttagtgtat accatctttt gccaacaaag tatagtatac ttttaagtat attatacttt
      181 aatgataaat caatcataaa atttaaaata attcatatta aagtttgtga atccttcaca
      241 aacttttctc gtattgcaaa tttgatattt aggaaaactc ccataaaaca acaaatgcga
      301 ttgcctctat tgagacaatc gcattttaac tatttaaaag attaacccat gccaccgccg
      361 ggatctccca ttctaaccaa taattgttct gaatcttttt taactaaatt atttgattct
      421 tcttttccac tagcaacagt ataaataatt ccagctgcta aaacaatact tgttaatatt
      481 aatattatct ttttcatata gcaccaccct aacatatcat accattttta ttaaattctg
      541 ccataatttt tttggggaac ttggaataga atctatcccc ttcacactcg aacagcataa
      601 tcgacttttc aattaaacta atatcatctt tcgctattcc taaataacaa tattgcatgg
      661 gagtcaaata accattttcc ttttctaaat cattaagaat gttaactgca tttatataat
      721 tacccttaac aatttctaat aaagattttt cagcgaaatg atatatttta atattttcta
      781 agtttttttt attcactaat ttaatgaatg catacgtatt aagaatcctt tgtcttctgt
      841 atttttctct ttcaaaataa tagggttcta attgttttaa tgactgtttt atatacctag
      901 atgcactatc ataacactca aaagtatacg actctgctaa atatacaaat gctgaagccc
      961 ttaaaagatg aaaacacccc tcgatattct ctatatctaa aatttcatga catagacttc
     1021 tcgatttttc aagattatct tgcactaaat acgcatatgc taagccttct ttaatcctac
     1081 ccaaatagga tgatttaata aaattatcag tgatttcttc aacttcaggc actaatacac
     1141 cagcatattc aaacaaagaa ttatattttt ttaaatcata cattgtgtag taagttaata
     1201 tgccatataa gaccttcatt tcttttgtct taataacctt acttccttta cgatcttcga
     1261 tcttagctaa taattcttgc tttttaatac taccactact tctaagccaa actaattcat
     1321 agacataagc ccattctcta ttcattgcaa gagatgaatt cttttcacta tctacaagag
     1381 atttcaataa ttgtaaatcg ccttttgcat ttgcgtattc catagcaatt cgtacatttt
     1441 ttttactcgt tgtaatagaa caaaaagtga acaacatttg acgtttttta ttaatatcat
     1501 tctcatacaa tatatttaat aattttacta aattccaaaa gctaaattga ctttttcctg
     1561 ttaaattctt tgaaaatgtc ccacctgaaa taccaatctc tttagcaagg tcttcctgac
     1621 tttttttttg aaaatctata tcatcaacaa gaacctttaa aaatcccact aatgtgttct
     1681 tttttggtac atcttctttc ttaacttttt gatccataac tattacccct tctgaaacaa
     1741 aaccttttcg ttcctatcat caagtaatac aatacataaa actgaaattc cccttgaatc
     1801 aatctaaaat attaattctg tatgtttata tatatttaat tcgctatatt ataccactat
     1861 tccccatata ttttcatgca attatacata aattgaaaat tattgtagtt taagggtttt
     1921 cccttgtttt tgggtgtgag tgctggctcg tcgtgtgggg acgaaccccc acaaagcgtt
     1981 aatatttatc gatagagaac aagattatga acggaagaac tggcggttcg gcgggtataa
     2041 ttgctagtcc aagtcgagca actcaacacc ccgtttttgg tctgttcggc ttggcgacaa
     2101 gtcaaaaacc cgccgaaaaa agtttcgcaa ggactgaata aaaaaagaaa ggcgttagcc
     2161 tttaggtgag gattaacgaa ccgcctttta ttattcagag ggtggagatt tttgaatgtt
     2221 tttttcaaaa atactacccg ttcctttcgg aacttttagc cctatatata agagtgagat
     2281 agagaaaatg aaaaaagtga gtaatatcaa gggtttataa gatatagggt gttacctttt
     2341 cgtaacacct gtgttacctt ttcgttacac ctaaaaaatg gaaaataata ataaaaaaaa
     2401 gatgtcaata tggaattcga catctaaaac aaataaattg gtaatttata ttttttctta
     2461 gaatcatcaa ctttaaaatt tctcaatgaa tttctaaaaa tatgttgagt tgctttatca
     2521 atcccatctt taggactaca acacataata ttaggattaa caaaccatgt acggcttgta
     2581 caagctcgtc catcttccgt tatcatccct gattcagcaa cccctaaaat ccctttttta
     2641 agtagaccat tcatcgttaa agaaatattc ttcctagcca ttttaaatcg ttctgctaag
     2701 tatgtaggag atgctggagt cgttgtatca gcattatctt tatcaatttg ttgaataata
     2761 acattagttt taaattctac atatggagca agcttaaata aaaaagcttc ttccttacta
     2821 ctcaaatact caagttcagt taaatagtta acattatcca taatcaactg cacaaacttc
     2881 acccgatccg taggtttctt tttcttgcca atataatatt cttctccacc agtcgcttta
     2941 cttaacagtt gtaaaagcat tcctctgtct tcttcagaaa aaccaccaga ctgcaattgc
     3001 tcaaatatat tatctatctt attttctcta tgttgtgctt ttttttcagc tttaactaat
     3061 tgtagttcta ctttgccgtt catctagatt cctcctcaaa attgaggtgg accaaaccaa
     3121 attacatttg aaatttcatt taaaaatcac cttaactatg gtaatctata aatagacaat
     3181 ttacggtgta ttcaacttat aaacaatcta tcaaaattaa ttaggttcga tacacacctt
     3241 cagaaaagag tcatgttccc gcatggctct tttttccatt ctaccacata tttttttaaa
     3301 aaaatgatgc aaaaagagtt tacatatctt tttataattt taattctgca agtctatatt
     3361 taggaacttg ctcccactct aactccaaaa ataattcctt accaaactta aaaaccttct
     3421 tatgcggttt atcattaatt aattttatct cttcatcatc ttttaccata ggtaacactt
     3481 ttttaattac aggcttctca acctcaacag aatcaacgtt caaggtctct aaaatggcgt
     3541 tttcttgctt tgtcacgttg ttcttttctt ctaatttctt ctcgattttt gtcttgctgc
     3601 tgttgttttt ctctagttga tctattctgt tcaacatttg ttccatcgac ttttgttggt
     3661 ctatcattaa ttctgttaat tgattcatta actctattac cattttgttt tgctctatta
     3721 atctttcgtt ttgttcgctc gaattgctct gtaatgtcgc catcatcaat tctacttgtc
     3781 gtaataaatt ttcgttcgtc atttctttct tgaatctgtc tgctaaactc actttctatt
     3841 ccctccttag taaagtcatc tcctagcgtc tttccacgta cccatttaga gacctcagga
     3901 tgcttatatg agagcgtttg tcctctcatc ttaactttga cacctaattc ttctaaagct
     3961 tctgaaaagc ccttaaaatc gcctgtacgc tcttttatat aattaattgc tcttcgcaat
     4021 tccattttcc aactattttt ccctttttcc tctaaacctt gctccgcttg tgtatagcga
     4081 atatctgctt ttctttccgt tacttctaac ccattttcta agcaaattct atcattctca
     4141 cttttaacaa aatgtctttg ttctgcatta ctttgatact tatttccagt ttcaatattt
     4201 actgaattaa tcacaatatg attatgaata tgtttcttat ctgtatgcgt ataaacagcc
     4261 acctgatggt ctttcgcaac cgctctagct aattctaacc ctaattcatt tgcttgctct
     4321 ggagtaactt cccctggtct aaatgattga ataattgtat gtgcttgcac cccatcgttt
     4381 tttccataga gcattctcaa ttccttaaaa cttgttttcg cataatccac atcacaattg
     4441 tggccacttt tcactgtagc ttttttttca gcatagttga tcgccctgct ggcttgcttc
     4501 gtatttccta atttagttgt tgccataatc gattcagctc tttcctcatt ccttcaacac
     4561 tatcattcaa tttctcccac tctacaccgc catgtcggtt cgagttacaa tatttagcga
     4621 tttggttcat gttgttcccc aacatgctca aatccctcgc aatcgattgt ctagttgctt
     4681 tatcaaattt cggcgcaact aatcgactgc cttgtgcctt ctttttcaca aaagttggca
     4741 cactcatatt taaagtttca gctgactgct gcaacttcaa atattcggat tcgctcactc
     4801 gaaagctaat ttgtctcggc tcttttcgaa taggttgctt ctgctcttta ttcgaatttt
     4861 cgctagccat attatcatct cgttcactca ttaaaacccg cccctttcaa ctctcgttga
     4921 catgttgtct catagaatca ttgtactacg tatctcgatt ctactcaaca acattagggc
     4981 ttggcaagcg tcgtatttgc tagccacttc gtggcgcaaa tacagcttgt tggggagttc
     5041 ccccaatccc ccttttgttt ttgcttacgc aaaaaagtga ctagcatctg ctagccacta
     5101 aaaacattta cccactctaa tcgcgggtaa atttattcct tcaaaaagtt gttctaccct
     5161 aagtagccct ccttttcttt gcccccgctt cgcaaaacgg actggctagc ccggacaggc
     5221 taacacaggg attctcatag taaagttcca ctacgttcca cttcctccac ttcgttccag
     5281 ccctctgggt acaatgagcc tccaccctgt gtatccttgc cagtgcgcca ctccttactg
     5341 cgaaggctaa ggggcaaagg tcgggcatcg ccctactcct atatgttacg aatatcggta
     5401 acatatatta aaatctttaa ccataaaaaa agagcaaagc cctagtgatc cgcactgact
     5461 tcgctcatat tttttctaca aggaattcca agtttcccta attctttttt acaattaggg
     5521 aaacttgacg atacccatta tatttacatc tattgatttt cttttaactt tttaatttca
     5581 agtaatagct tttgattttc tttttctaag gcttcattcg tattaataag cacattagca
     5641 cctttacgaa tactatttaa cctaagtaat tctacctcgg aaatttgata cgttacacct
     5701 ttatcagttt caactttttt tatatatttg atccctggat tagtttcgtt tttaagatag
     5761 tctttagccc attccaattg atttttcaca aaattatatt cttgctgcaa ggactgtttt
     5821 tcttttttca tctgctcaat ttcttgagcc atctctcttg taagctgacg aacttcttct
     5881 acttcctctt tagttcgaat atcctttgca atcgttgttg taccacgttc taaatctatc
     5941 ccttttttct gtagaaacca aggtaattca ttttgtatat atactaattc ttttctatca
     6001 aaaatgtttt tagcttgtaa tcttccatct ttcattggaa ctattccaac taacatataa
     6061 ggcgtatcac catcaaagtg tacttgtgca tatgcgatat tttgtttacc atatcttcct
     6121 tcgaagaaat taacgacata ttgaaaaaac tctttaattt tatcaaattc aatgacttca
     6181 aaataatcat tagaaactga cacataccat tgagaaacca aaaccgcgtc ttttctaatc
     6241 gttaacaatg aatctctttg ctcattgatt atctccataa ttctactttc atgatttacc
     6301 ggctctttat taactaaatc ataagcacat tccttcggaa tttcattcat attggcaaac
     6361 ttaatcttct ttcgcattga tgctatcaag tacttcactt aaatctctcc tcaatcattc
     6421 atttcttctt aagcgatccg tccgatttct tttgattcct ctttgtctta gaccagaagc
     6481 tcttttttct acttttgttt cttgcaccgc acgttggctc tccgctcgta ccgtaccacc
     6541 tgatactgtt ggattcactc cctgtacgac tttattctct acttttgttt cttgcaccgc
     6601 acgttgactc tccgctcgta ctgtaccacc tgatactgtt ggattcgctc cctgtacgac
     6661 tttattttct acttttgttt cttgcaccgc acgttgactc tccgctcgta ctgtaccacc
     6721 tgatactgtt ggattcgctc cttgtaccgc tttattctct actttcgttt cttgcaccgc
     6781 acgttggctc tccgctcgta ctgtaccacc tgataccgtt ggattcgctc cctgtacgac
     6841 tttattctct acttttgttt cttgcaccgc acgttgactc tccgctcgta ctgtaccacc
     6901 tgatactgtt ggattcgctc cttgtaccgc tttattctct actttcgttt cttgcaccgc
     6961 acgttggctc tccgctcgta ctgtaccacc tgataccgtt ggattcgctc cctgtaccgc
     7021 tttattctct acttttgttt cttgtaccgc acgttggctc tccactcgtg ccatgccacc
     7081 tgataccgtt ggatttcctt gctccacttg tttcttctca acattcatgg tttgtattct
     7141 tctcaaattt tcatcttgcg aagcttgtcc tgaagcttgc gaattccctt gttccacttg
     7201 cttcttctca atattcatgg tttgcattct tctcaagttt tcatcttgcg aagcctgtcc
     7261 tgaagtttgc ggattccctt gttccacttg cttcttctca atattcatgg tttgcattct
     7321 tctcaagttt tcatcttgcg aagcctgtcc tgaagtttgc ggattccctt gttccacttg
     7381 cttcttctca atattcatgg tttgcattct tctcaagttt tcatcttgcg aagcctgtcc
     7441 tgaagtttgc ggattccctt gttccacttg tttcttctca acattcatgg tttgcattct
     7501 tctcaagttt tcatcttgcg aagtttgtcc tgaagcttgt gggtttcctt gttctacctg
     7561 ttgtttctcc acttgcatca tttgtttgct tcttaaaatt tcatcttgtg aagtttgccc
     7621 tgggctttgt gaattacctt gttccatttg ttgtttattt gcatttgaaa cacttcccat
     7681 ttttggtgaa acgttttgat cctcaccgga attggcatta ttgtgaatgt ctttacctaa
     7741 tttatttaac aattgacggt cattattttc ttgagccttt tgttttcctt cggcttggcc
     7801 ttctttaaat tcatcgatag tattttgcac accacttgaa acaccttttg taacacccga
     7861 tgccttttct ttccctttac caacagcact actcatagta gctacaacca tattacctgc
     7921 atcattcatc atgccaggca cacctctatt agttgcataa cttaacgctg caccggtatt
     7981 tttcataaaa ttctttggca taactcctga ttttttatta tctttatcat tcaaaccatt
     8041 tactttatcc tctgaatcca tttcagaacg tttaggagta tcatctcttg attgttgagt
     8101 agacgcggat tcccttgcac cactaaacat attcttcatg ccttcaccag ctcgtttagc
     8161 cagcgataac ccaccaaccg ttgccaagaa accattcata ccttctttta aaccaacatc
     8221 tacaccaaaa tattttaaaa ttgtatttga tcctttaatc agagcaattg ttaaacaaac
     8281 taaaacaact acatacaata caggatttaa atccttattc gccacaaaat taactgatat
     8341 tgtgaaaaat cttaatgaaa gcccagtaaa tgcaatcgtt aaaaatcctt gcccaatatc
     8401 ctgaatcatc attttagttt tctgaccaga atctatatca gtaaccatta caaatggtgc
     8461 tactaacttt ttcataacta attcaaaaat tgttattaca aaaacaaata gcgtaaataa
     8521 atatgcaact cccaaagcta ataaagacca aaagatagtg aaaaattgca taggatagcg
     8581 cttatatcct gattcaaaag aatccttaaa cggattaaac atactagcct tttctatctt
     8641 actaaccgtt tgttctttcc cagtatgaga aatcttatat gttaaataat ttgtctcggc
     8701 tgccatgcca gttccttctt ttgaaatatc ctcaattacc tttaaagtta ataaatcact
     8761 taaatcagct tgtaagaatt cttctttaga caaattattt ttcggttctt ttgaattttc
     8821 ttttttaatc gactcaaatc ccacctgact taaatataac aaatcagctg tattatcttt
     8881 tacaatgtcc caagctaaac cttctttagc ttttccctgt tctactgatc ctttataaaa
     8941 atcagtagcc atactttgca tatcagccat taattcattt aaaccaccta ataaaccaat
     9001 cattataact acatttacac ctacggattt aaatttaggt ggattatgtt gaattatgac
     9061 tctaattcct atccaaacaa tcattaatac taaaattgaa tagaacaaac ctttcataat
     9121 agatgtataa aattcattca atccagcatc ttttaataaa tcaaaaaggg aaaatgtatc
     9181 aggaatcaat ccttcaacca aggtaacaaa actcaataaa agttttacta taccacctaa
     9241 aaatgattgt aaaattgcac ctaaaaaatc agtataacgt atccaatcat tatgtaaaaa
     9301 ctctagtatt ttgggaatat cagaatcctt tgttccaaaa aaatctgacc aatcccaatc
     9361 agcataaacg gtaaatggaa ccgttataat tgccattatt aatgatacaa caaacgaata
     9421 taatatcttc tttttatttt tcatatactt ctccttcttt ctttattcta aaaaagtata
     9481 gtagacttta aggtctacta tactttaata attaaaaaaa acgactaaat acatctacac
     9541 caaaagcaat tacaacaaaa acaataaaga aaccaataag tattactaca caacctaagc
     9601 taatttgaaa cattcgaata agaatataac ccacaaaagc taaaaatatt aatgctccaa
     9661 tttctgataa tggtatccca aataaaaata ataaaagcat taatccaaaa aataataacc
     9721 atcctttttt caattagatc gccaccctcc gcccttataa aaacttaaat acaggcttta
     9781 taaggttcaa tttcataatc ctctattact ggttgcatat actctccaaa ttccatacat
     9841 tcttcttcat attcatcaat agattcaata atttgtaact gttgttctaa atcataattt
     9901 gaaaaatcat ctaccatttg ttcaactaca attctttcat ccacctttct ttttaataga
     9961 taatccaata cttttttctc aattcgtaac acaaatctat taagaactct taaaaatgaa
    10021 cttttcggat tctcatattc ttttctcaaa ctatcagaag caaatttgaa actgtcatac
    10081 gtttccattt cttcgaatgt cctattcatc ttgctagtta attgtgttac acttttatca
    10141 atgacgtggt tcataatact gaatcattcc tttcaagggg tacagaccgc aaaacggaaa
    10201 aaaaccacgc taagaatttt cgagccattt taacgctaat ttaaattgtg ttgtattcga
    10261 aattagatca tttcgataaa ttgtgttgcg ttaacaccta gtttcattag tcttttttag
    10321 ttctaatgca acacaaatga cattgtgttg tatcaaaatg ttcttaacg
//
