LOCUS       NZ_CP072700            11839 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain IMBL-B9 plasmid pKBT11, complete
            sequence.
ACCESSION   NZ_CP072700
VERSION     NZ_CP072700.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18221107
            Assembly: GCF_023921665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Park,M.G., Choi,J.Y., Kim,J.H., Park,D.H., Wang,M., Kim,H.J.,
            Kim,S.H., Lee,H.Y. and Je,Y.H.
  TITLE     Isolation and molecular characterization of Bacillus thuringiensis
            subsp. kurstaki toxic to lepidopteran pests Spodoptera spp. and
            Plutella xylostella
  JOURNAL   Pest Manag Sci 78 (7), 2976-2984 (2022)
   PUBMED   35419912
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   Park,M.G., Choi,J.Y. and Je,Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2021) Department of Agricultural Biotechnology,
            Seoul National University, 1 Gwanak-ro, Seoul 08826, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023921665.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 14:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,736
            CDSs (total)                      :: 6,585
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 118 of 328
            Pseudo Genes (incomplete)         :: 239 of 328
            Pseudo Genes (internal stop)      :: 81 of 328
            Pseudo Genes (multiple problems)  :: 86 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072700.1.
            Bacteria and source DNA available from Laboratory of Insect
            Microbiology and Biotechnology, Seoul National University, Republic
            of Korea.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="IMBL-B9"
                     /db_xref="taxon:1428"
                     /plasmid="pKBT11"
                     /geo_loc_name="South Korea: Suwon"
                     /collection_date="2018-04-13"
     gene            51..719
                     /locus_tag="J2N67_RS33230"
                     /old_locus_tag="J2N67_006694"
     CDS             51..719
                     /locus_tag="J2N67_RS33230"
                     /old_locus_tag="J2N67_006694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000172520.1"
                     /translation="MTLIDQMKKEIAELKGSIQRDKLQAINEKIAKQEAKEKGNDLAKR
                     EREEKAKAEAMARAFDGGLNYEDVATGNINTKVIETVSPELASLYKERDELTALLNEST
                     ADVLGDLNEHEQATHDIANKQVERTAVANGSSTVELTPVQAPPVDYTALSMDMAIIPGA
                     NPRNLFEKQAGMSFERYTQIATPEEVQKANAFVRPNATKQAQHEQNLQSGAYNTGRIEF
                     "
                     /name="hypothetical protein"
     gene            785..1036
                     /locus_tag="J2N67_RS33235"
                     /old_locus_tag="J2N67_006695"
     CDS             785..1036
                     /locus_tag="J2N67_RS33235"
                     /old_locus_tag="J2N67_006695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000221333.1"
                     /translation="MTYKIQTDNGYIQLQVTGELTGEAYKPFFDTMADNIEDACKRRIQ
                     EGEHVSIRSYKMKKRVSAKGTNAGNNRKAWMKEKLKGE"
                     /name="hypothetical protein"
     gene            1044..1517
                     /locus_tag="J2N67_RS33240"
                     /old_locus_tag="J2N67_006696"
     CDS             1044..1517
                     /locus_tag="J2N67_RS33240"
                     /old_locus_tag="J2N67_006696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000030291.1"
                     /translation="MSKAKRLLREVRRMKASIVPNTAVFIVNCIEQIKGIDPRKKAKEN
                     IVIIDNVDQDCINCTKHFSECKCDESSYRPPTENEYAYWKEKSPRHYERLMRLRDQDEQ
                     YEKTYGLDKWIEDNVLYVRKKKVNGATDKIDQIPLSQMSDEMREVLCKDVKRI"
                     /name="hypothetical protein"
     gene            1501..1818
                     /locus_tag="J2N67_RS33245"
                     /old_locus_tag="J2N67_006697"
     CDS             1501..1818
                     /locus_tag="J2N67_RS33245"
                     /old_locus_tag="J2N67_006697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000063381.1"
                     /translation="MSNAFKPTYMTSNDYVRSKEDIKALERELGMTPGQLYKTRWTDIK
                     TLDMAGKLHENDMNVLFTRMKVYDPSLYDYVLNSECQIVHKSELYDNQMRERARRIRNL
                     L"
                     /name="hypothetical protein"
     gene            1835..2203
                     /locus_tag="J2N67_RS33250"
                     /old_locus_tag="J2N67_006698"
     CDS             1835..2203
                     /locus_tag="J2N67_RS33250"
                     /old_locus_tag="J2N67_006698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000179528.1"
                     /translation="MATAKKKETTKEPINIDIKIATPKQALATRKLIMDMICRREIEQS
                     DSRQLIYALQGASVEHRSMQEGEKLKIEREKVAIQERAVKVSEEMKLKLEKDNEQLEEE
                     VSELREILKERDERGQRY"
                     /name="hypothetical protein"
     gene            2837..3058
                     /locus_tag="J2N67_RS33255"
                     /old_locus_tag="J2N67_006699"
     CDS             2837..3058
                     /locus_tag="J2N67_RS33255"
                     /old_locus_tag="J2N67_006699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001291044.1"
                     /translation="MSEKKSKFETFYTNKMDYYGVDNIKDAEKAYKMERMIAKNIVEFG
                     IDKVEEWIELSKITGVKDRIKEKLKKEK"
                     /name="hypothetical protein"
     gene            3128..3316
                     /locus_tag="J2N67_RS33260"
                     /old_locus_tag="J2N67_006700"
     CDS             3128..3316
                     /locus_tag="J2N67_RS33260"
                     /old_locus_tag="J2N67_006700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001058100.1"
                     /translation="MNNDKKQLLESKFFWLVNGLNEMLEEANPNEGETATLDNVNIDHL
                     LECVQLMHVVTNQLQNR"
                     /name="hypothetical protein"
     gene            3794..4741
                     /locus_tag="J2N67_RS33265"
                     /old_locus_tag="J2N67_006701"
     CDS             3794..4741
                     /locus_tag="J2N67_RS33265"
                     /old_locus_tag="J2N67_006701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_252598130.1"
                     /translation="MLNIEKTVHYYNYAQQYLMMPKAVEEYLAIHDVSLGACKLYMHLR
                     DRYRLSLKSVSEGNDKYEDELGVFCIFPIEDLVEIMGKNEKTVRRWYKELKECDLIQYR
                     QVKKESGQANRFYIKEEQLVDSGLIALYKSEEYKPVVHYPQIGSDKNVQSTSGQKCPPI
                     NNSYLINNKIYSEFLSNSHEFLTTEEAEKIVRKFKGDLDQEPEDNKHFLKFANACYERL
                     CNHDVDPHETWKEHNEKQGAGYVGNQDIPEDTYNQFVDALNDNEGKSITDSKKSHIEGN
                     KKPYLIVNNVPKWGTNRETEKNHAKDFRAILTLI"
                     /name="replication initiator protein A"
     gene            5029..6465
                     /locus_tag="J2N67_RS33270"
                     /old_locus_tag="J2N67_006702"
     CDS             5029..6465
                     /locus_tag="J2N67_RS33270"
                     /old_locus_tag="J2N67_006702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS231A family transposase"
                     /protein_id="WP_001053955.1"
                     /translation="MNLSIQDELQLFSEELCRHLTPSFLEELAKKLGFVKRKRKFSGSE
                     LATICIWISQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSILWKG
                     KLCKTSAISSTALTHFQRIRILDATIFQIPKHLASIYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQVGPGKNNDKTFGTECLDTLRPGDLCIRDLGYFSLEDLDQMDQRGAYYISRLK
                     LNHTVYIKNPSPEYFRNGTVKKQSQYIQVDLEHIMNHLKPGQTYEIKEAYIGKNQKLFT
                     RVIIYRLTEKQIQERRKKQAYTESKKGITFSEKSKRLTGINIYVSNTPEGIVPMEQIHD
                     FYSLRWQIEIIFKTWKSLFQIHHWQNIKQERLECHVYGRLIAIFICSSTMFKIRKLLLQ
                     KNKRELSEYKAIGMIQDHVSLLYQAIQRNTQDLTKILIRLFDLLQKNGRKSHRYEKKTI
                     FDIMGVVYEYNGFGKQKKAA"
                     /name="IS4-like element IS231A family transposase"
     gene            8133..8249
                     /locus_tag="J2N67_RS33275"
     CDS             8133..8249
                     /locus_tag="J2N67_RS33275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157457025.1"
                     /translation="MADIEKLMGYSSEEFSLLNFQDSKYINERNRRKRKPQF"
                     /name="hypothetical protein"
     gene            8212..8439
                     /locus_tag="J2N67_RS33280"
                     /old_locus_tag="J2N67_006704"
     CDS             8212..8439
                     /locus_tag="J2N67_RS33280"
                     /old_locus_tag="J2N67_006704"
                     /inference="COORDINATES: protein motif:HMM:NF021206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="WP_002084286.1"
                     /translation="MKEIGEKGNRNFEESSYNLGNGRNYKKYNMTKDGFTLLVMDENAK
                     IRFLLVGSDVLNFEPIKEKETRNREQIKYN"
                     /name="Rha family transcriptional regulator"
     gene            8550..8672
                     /locus_tag="J2N67_RS33665"
     CDS             8550..8672
                     /locus_tag="J2N67_RS33665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="WP_267865592.1"
                     /translation="MEYKDQWNRKQVKYNLFKDGFTLFVMGYNTREAVQMKPIH"
                     /name="Rha family transcriptional regulator"
     gene            8911..9471
                     /locus_tag="J2N67_RS33290"
                     /old_locus_tag="J2N67_006705"
     CDS             8911..9471
                     /locus_tag="J2N67_RS33290"
                     /old_locus_tag="J2N67_006705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000225071.1"
                     /translation="MVADIKNFTIGVLGSLFATFLTTYIIHFEQFKGSFNVKEIWNIVA
                     NYTFLIYWIIALIILIITRRIIRGRLNKSQTPYPMVFSIGGSHDAEFNAEGHGFKWKAY
                     ADVKRRDRSTNEPLDIQVDRVKGPYCMNDFREMKVSRTYWGRYKYKCPKCGYKRILLKN
                     AWTLKCDIGDEIEAGYRDKVNTR"
                     /name="hypothetical protein"
     gene            complement(9551..9697)
                     /locus_tag="J2N67_RS33295"
                     /old_locus_tag="J2N67_006706"
     CDS             complement(9551..9697)
                     /locus_tag="J2N67_RS33295"
                     /old_locus_tag="J2N67_006706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000247102.1"
                     /translation="MVNGLGMTLEQQQRFFDPIIKFIKENPHLFDLVPKQSNSRTQEQT
                     SEK"
                     /name="hypothetical protein"
     gene            complement(10156..10560)
                     /locus_tag="J2N67_RS33300"
                     /old_locus_tag="J2N67_006708"
     CDS             complement(10156..10560)
                     /locus_tag="J2N67_RS33300"
                     /old_locus_tag="J2N67_006708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="WP_000642527.1"
                     /translation="MKADKKAIVNKPIVVGTLPQVIIDKYELECDSNDVKMYPGAIKHI
                     QRKHPGIYEQYSGNIKDIIENPDYVGNNPKEPNSVELIKVIDEHILIAIKLDPSGYLFL
                     SSMYDMNNGPVKVEKRLKSGRLQPYMDLIG"
                     /name="PBECR2 nuclease fold domain-containing protein"
     gene            complement(10857..11573)
                     /locus_tag="J2N67_RS33305"
                     /old_locus_tag="J2N67_006709"
     CDS             complement(10857..11573)
                     /locus_tag="J2N67_RS33305"
                     /old_locus_tag="J2N67_006709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345488.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_000345488.1"
                     /translation="MDFGRFSGQVSKELDVNGNTLRVWCLELESAGYKFERNNRQQRIY
                     YEHDITILKEMKVLMADGTRTLQEAISLLLESSLSKDTDNALTHSINDSDGSEITPLQR
                     NNSAVGVAYAILEQTNEKVAELIKQQQIIALQNQEILNVIHKDNEEKQLVIQAVFEKQQ
                     QLIEQQQDIHLQNQNLTKMYLDEREEKLKERQEKNELKEQLSDMQEKMNEMLKFVHQQS
                     EERSKSFLSKFFSKKT"
                     /name="MerR family transcriptional regulator"
     Typing          3795..4673
                     /name="rep_cluster_899"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caagttgtta gacaatttaa tacaacaatt aatgaaagaa ggttatacat atgacactta
       61 ttgatcaaat gaaaaaagag attgcagagt taaaaggtag cattcaacgt gataaattgc
      121 aggctattaa tgaaaagatc gcaaagcagg aagcaaaaga gaagggcaat gacctggcta
      181 aacgcgaacg agaagaaaag gccaaggctg aagcaatggc ccgcgcattt gacggcggtt
      241 taaattatga agacgtagcg acagggaata ttaatacaaa ggtaatagaa acagtaagcc
      301 cagagttagc aagcctatac aaagagcgtg acgagttaac agcgctatta aatgaatcga
      361 cagccgatgt actaggcgat ctaaacgaac acgaacaggc gacacatgat attgctaaca
      421 aacaagtgga gcgcacagcg gttgcaaatg gatcaagtac agtagaactt acaccagtac
      481 aggcgccacc agttgattat acggccctat caatggatat ggcgattatc ccaggggcaa
      541 acccgcgaaa cttatttgaa aaacaagcgg gcatgtcatt tgaacgatac acacaaattg
      601 caacaccaga agaagtacag aaagcaaatg catttgtaag accaaacgca actaaacagg
      661 cccagcatga acagaattta cagtctggtg catataacac tggccgtatc gaattttaag
      721 agtcgttacg ataagggtag ggatttcact cttactcttt tattaataaa aaggaggaat
      781 ccttgtgaca tataagatcc aaacagataa cgggtatata caattacagg ttactggaga
      841 gttaacagga gaggcataca agcccttttt tgatacaatg gccgataaca tagaagatgc
      901 atgtaaaagg cgtatacagg agggcgaaca cgtttctatt cgatcataca agatgaaaaa
      961 gagagtaagt gcaaagggaa cgaatgcagg gaataatcgt aaagcgtgga tgaaagagaa
     1021 attaaaaggg gagtgaaggg aacgtgtcaa aggctaaacg attattaaga gaagtaagaa
     1081 gaatgaaagc cagcattgtg cccaatacgg ctgtatttat agtaaattgt attgaacaaa
     1141 ttaaaggaat tgacccccga aaaaaggcaa aagagaatat tgttatcatt gataacgtgg
     1201 atcaggattg tattaattgt actaaacact ttagcgagtg caaatgtgac gaatcaagct
     1261 atagaccacc aacagagaac gaatacgcat attggaaaga gaaaagcccg cgccattatg
     1321 aaaggcttat gaggttacga gatcaagacg agcaatacga aaaaacatat ggtttagata
     1381 aatggattga agataacgtt ttatatgtaa ggaaaaagaa agtaaacgga gctacagata
     1441 agattgacca gataccttta agccaaatga gtgatgaaat gagagaggtg ttatgtaaag
     1501 atgtcaaacg catttaaacc aacatatatg acaagtaatg attatgtacg atcaaaggaa
     1561 gatataaagg ctctggagcg cgaactagga atgacgccag ggcaactata taaaactagg
     1621 tggacggata taaagacgtt agacatggct ggtaagctac atgaaaatga tatgaatgtg
     1681 ctgtttacta gaatgaaggt ttatgatccc tcgttgtatg attacgtatt aaacagtgaa
     1741 tgccagatcg tgcataaaag tgagttgtac gacaatcaaa tgagagaaag agcacgacga
     1801 ataagaaact tattatagag aggatagatg gaacatggca acagcaaaga aaaaagaaac
     1861 aacaaaagaa ccgatcaata ttgatattaa aatcgctaca ccaaaacaag ccctagcaac
     1921 acgaaaatta ataatggata tgatatgtag aagagagata gaacagagtg actctagaca
     1981 gttaatatat gctctacaag gtgcatcagt ggagcataga agcatgcaag agggtgaaaa
     2041 actgaagatt gaacgtgaaa aggtagcgat ccaggaaagg gccgttaaag tatcagaaga
     2101 gatgaagcta aaactagaaa aagataatga gcaattagaa gaagaagtat ccgagttaag
     2161 agagattcta aaagaacgag atgaaagagg gcaaaggtac taagttttat cgggtgttta
     2221 tccagggttt ttaattctga acgcgagaag attcgcgcac acatgacacg caaccccccc
     2281 taccctatat aagtatataa aatatgttat aacagatcac tgaccaggtg atataatact
     2341 tatattgaac acctttgata taaatatgtt agaaacttac taaaagttag ggttataata
     2401 agtattatgt aaactaaatt agtaacaaga atgctgttat atcaaggttt atgtctattc
     2461 gaaaaaacac gaacgttttt acagtcattt ttagatgagc tttgtatgac gatatagtgg
     2521 ttgttgtaag ataattggaa aaaacggtca ttattaccga acattataag cgttataagg
     2581 ttacattgtt cggtgtaatg aatagtgtat tttataacgt tttctacaaa gtcagttgaa
     2641 aggacttatc ctaattgggt atgccttttc tttttggctt aaaaggtaat tggatagctg
     2701 attctgatca acagacttat ttcttatcaa tctttttcag tcggaatatt aaacaacacc
     2761 aaaaaaagtt tcgaaaccac atttacaagg tttcgaaact gttatatact attcactgta
     2821 aaggagagat gaacaagtgt ctgaaaagaa gagtaagttc gagacattct atacaaataa
     2881 gatggactat tacggtgtag acaacattaa agatgctgaa aaagcatata agatggagcg
     2941 tatgattgcg aaaaacattg tagaatttgg aattgataaa gtagaagaat ggattgaact
     3001 atccaaaatc actggagtta aagatcggat taaagaaaaa ctaaaaaaag agaagtaaac
     3061 taaagggcaa tggcccatct cttttttatt aaaatggttt cgaaaccgaa attaggaggt
     3121 attcaagatg aataatgaca aaaaacaatt actagagagc aagttttttt ggttggtaaa
     3181 cggattgaat gaaatgctgg aagaagctaa tccaaatgaa ggtgaaaccg cgacgttgga
     3241 taacgtgaac atcgatcacc ttttagagtg tgttcaattg atgcacgttg taacaaatca
     3301 attacaaaat agataaagcc cactactggc atagtgagct agaaaggagt ttgcaaatgt
     3361 cattcacaac agcaatgtgg ttcatgttaa cattcgcaag tccagtatta agtatattgg
     3421 cagaggaaac ctcaaaattg aggtttcctc agtgagagac gggctcaatt ttgagctcgt
     3481 ctctttacct tgttcaatta aatattaagg gtatacgaaa aagtgcggta tcaacaaaaa
     3541 gagttcctct gagaaccttt ttttgtttag tagattcata gtataatagg gtatagacag
     3601 ctaaaaaccc gctatatcct tcaccgacca aagttaggat acaacgggtt gatgcatacc
     3661 agggatttct cccttactca ctgattaatt gcagtaatta ctcactatgt atattatagc
     3721 aatttcatag tataggaaca agtgaaatct ctgtttagtt gtcgtactta aatgacaata
     3781 atcggaggtt tttatgttaa atatcgaaaa gacagtacac tactataact acgctcaaca
     3841 gtaccttatg atgccgaaag cggttgagga gtatcttgct attcacgatg ttagtttagg
     3901 ggcttgcaag ttgtatatgc acctcaggga tcgttacaga ctatcattaa agagtgttag
     3961 tgaaggtaac gataaatacg aggacgagtt aggtgtattc tgtatttttc ctatagagga
     4021 tttagttgag atcatgggca agaacgaaaa aaccgttaga cgatggtata aagagttaaa
     4081 agaatgtgat cttattcaat acagacaagt caaaaaggaa agtggacaag ctaaccgttt
     4141 ctatattaaa gaagaacaat tagtcgattc tgggttgatc gcattatata aaagtgaaga
     4201 atacaaacca gtcgtacatt atcctcaaat aggatcggac aaaaatgtcc agtccacgag
     4261 tggtcaaaaa tgtccaccta ttaataacag ttatttaatt aataacaaaa tatattctga
     4321 gtttctttca aactcgcatg agtttttgac tacagaagaa gctgaaaaga tagtcagaaa
     4381 gtttaaaggg gatcttgacc aagaacctga agacaataag cacttcttaa aattcgctaa
     4441 tgcgtgttat gaaagattgt gtaatcatga cgttgatcca cacgaaacgt ggaaagaaca
     4501 taatgaaaaa caaggtgcag ggtacgttgg taaccaggat atacctgagg atacttacaa
     4561 tcaatttgtg gatgctttaa atgataatga ggggaaaagt attactgata gtaagaaatc
     4621 gcatatagag ggaaataaga agccttattt aatagtcaat aacgtcccta aatggggtac
     4681 aaaccgcgaa acggaaaaaa accacgctaa ggattttcga gccattttaa cgctaattta
     4741 aattgtgttg tattcgaaat tagatcattt cgataaattg tgttgcgtta gcacccattt
     4801 gcattggtct tttttagttt taatgcaaca caaatgacat tgtgttgtat caaaatgttc
     4861 ttaacgggac aaaacttaaa gaattttatg ttgaaatttg cttaacgtgg tttatttcca
     4921 aaatgctggc ggtaccccat gcccatcaac ttaagaatct agactacccc caagtgtaaa
     4981 aaaacgttat tctttctaaa aagctaacta gaaaggatga cgttttttat gaatctttcg
     5041 attcaagatg aattacaact attttctgaa gagctgtgcc gtcatttaac cccttctttt
     5101 ttagaagaac tcgcaaaaaa attaggtttt gtaaaaagaa aacgaaaatt ttcaggaagt
     5161 gaattagcta ccatttgtat ttggataagt caaaggacag cgagtgattc cctcgttcga
     5221 ttatgtagcc aattacacgc agctacaggt actcttatga gtccagaagg acttaataaa
     5281 cgctttgata aaaaagctgt tgaatttctg aaatatattt tttctatatt atggaaaggt
     5341 aaactttgta aaacatcggc tatttcaagc acggcgctta cccactttca acgtattcgt
     5401 attttagatg cgacgatttt tcaaatccca aaacatttag ctagtatata tcctggatcc
     5461 ggtggttgcg cacaaacagc gggtatcaag attcaattag agtatgattt acatagcggt
     5521 caatttttaa actttcaggt aggtccaggg aaaaataatg ataagacctt tggaacggaa
     5581 tgtttagata ccttacgtcc aggtgacttg tgcattcgtg atttaggtta tttttcatta
     5641 gaagacttag atcaaatgga tcaacgaggt gcctactata tatccagatt aaaattaaac
     5701 catactgttt atataaaaaa tccgtctcca gaatactttc gaaacggaac cgtaaaaaag
     5761 caatcacagt atattcaagt tgatttagaa catatcatga accatttaaa acccggtcaa
     5821 acatatgaaa taaaagaagc ttacattgga aagaatcaaa aactattcac gcgtgtaatt
     5881 atctatcgtt taacagaaaa acagatacag gaacgtagaa aaaaacaagc ctatacggaa
     5941 agtaaaaagg gcattacgtt ttcagaaaag agtaaacgat taacgggtat caacatatat
     6001 gtctcaaata cgcctgaagg gattgttccg atggaacaaa tacatgactt ttactccctt
     6061 cgctggcaaa tagaaatcat ttttaaaact tggaaatcct tatttcaaat tcatcattgg
     6121 caaaatatca aacaagagcg attagaatgt catgtgtatg gaaggcttat tgccattttt
     6181 atctgttctt ctacgatgtt taagatacgt aaacttctgt tgcaaaagaa caaaagagaa
     6241 ttaagtgaat ataaagctat tgggatgatt caagatcatg tatcgctgtt atatcaagcg
     6301 atacagagaa acacccaaga tctaacaaag attttaatcc gtttgttcga cctcctacag
     6361 aagaatggac ggaaatctca tcgatatgag aagaaaacta tctttgatat tatgggtgtt
     6421 gtctatgagt ataatgggtt tggaaaacaa aagaaagctg cataattttt aaaatgaaac
     6481 tcgttagagt ttatttggtg tgtgtacttt tcaataccaa aagtacatta cgagtttttt
     6541 agttgctttg aacgaaagtt cattttattt ttgttcttaa gttgatgggc atgtggcggt
     6601 acccctaaat taacaggtaa acaatttgat aaattaatag ttttagtgag aggggcgaat
     6661 agcggcgatc ctgtagcgtg gaaaaaatgt gaatcaccag aaatgcaaag ttatgtagaa
     6721 aagacagaca accgttttaa gattagaaac gcggttatta tgggcatgta actgttaaag
     6781 taatataaat acatttttat gatataaaaa gcgttagaaa tggaaatgag aggtttaaga
     6841 gggtgttctt aattttaagc gtaccctcta tatatggggt acgaaaaagt tcacatacct
     6901 ggccatcctc caaagttgga gagtgagttt tatgtcgcaa atattaatgt ttctggtgaa
     6961 ccttatcaaa ttttcgttga tttaatagaa acatagcggt aaaattagca gtaacttaat
     7021 agaacggaaa tgaaaaaagc cactctcata tgctattggc taccaacctt tagcgagaat
     7081 gacttaatcc tgtacagcca tacaggactt cgacttataa gaggcgccaa cctcaaataa
     7141 gttatttgcc ttgttttcgc gaacaaggct tattagatac acctattgta ccgttactct
     7201 acgaatattt caactagtaa ttactagcat tgtcatatac ataataaaac ggatataaaa
     7261 gggcgttttc tatacctaga agtcttgtaa atgtacaggg cgtttagata tagagaacgc
     7321 cctttttgtg ttccgttcca gtggaagcta ccactttaaa aagatggtct agtgtagcca
     7381 atgcaggaga gtacactcgg atatcagttg tcgttgcatt caactgtctg acgtaagcga
     7441 ggtaaaggac acaagccttg cataaaacaa gcctacggga tgtaaatcct aataacgatg
     7501 ataaccaaga cgttagcggc aaaaagtgtt gggggttcaa aataagacat gattgtgcga
     7561 ctggagttaa tcagttactc gtaagcggcg atcatgacac tgattcacgg ctattcttgt
     7621 acaagtttta tcacaaggat atgcgggtta tatagcgaat catccgaaag ggaacggtgt
     7681 tgggcgtgag aaacgcaccg tacggcgaaa tacaaagccg ataagctata tacggacggt
     7741 atagtagttt tataaactat aaccgtttgt cgtcaatgca accaatctca attcgagacc
     7801 tcggcatcta agccagtacg aatgagtggg cgttttaacc tcgtaaattt tcaacagggg
     7861 ttactatgcc caaaactaca ttcaaatttc ctaacaagct cgccagtatg aagagcgtaa
     7921 gaccttaaag tcaagggatt cagctttaat tgcttaaaaa aattagagaa ctgaaacaat
     7981 cactattaat taagatagta gggtaaagat tatggattat ttttaatata ggggtaatta
     8041 gtgacaaact tagtatttgt aaacaataac aatgaggtaa taacagattc attaaagtag
     8101 ctgaaatttt tggtaaacgt catgacaatg ttgtggctga tattgaaaaa ttaatggggt
     8161 attcaagtga agaattttca ctccttaatt tccaggattc aaaatacatt aatgaaagaa
     8221 ataggagaaa aaggaaaccg caattttgag gaatcctcat ataaccttgg aaacggaagg
     8281 aattacaaaa agtacaacat gactaaagac ggttttacat tactggttat ggatgaaaac
     8341 gcaaaaatac gttttctact agtgggaagt gatgtgctca attttgagcc gatcaaagaa
     8401 aaagaaacta ggaatagaga acaaatcaaa tataattgaa gttcttaaag ggattggcta
     8461 caaaagggta tcagcaactt cgctgacact acttacattc atgaacaaaa caaacaactt
     8521 tataaaaagg tatttccttt tttgagccga tcgaatataa ggatcagtgg aatagaaaac
     8581 aggttaaata taacttgttt aaagatggtt ttactttgtt cgtgatgggt tacaacacta
     8641 gagaagctgt acaaatgaaa cctattcatt aaatcaatta ctgatagtta actcacttac
     8701 taaaagtaag attatccatg agtagatatt ataagatttg ttttttgttg ttatatatag
     8761 taatttagat gaaattcgat gttgtttgat gtaaaagtat taattaattt aaatatgaga
     8821 ttggaagctt gtacaacttt tgtatattta catgtatact aaaagaatag ttaaatattc
     8881 tttattttca tatgaggaca ggtgtcgttt atggttgctg atattaaaaa tttcactatt
     8941 ggtgttctgg gttctttgtt tgctacattt ttaactacat atataattca ttttgaacaa
     9001 tttaaagggt catttaatgt caaagagatt tggaatatag ttgctaacta tacattccta
     9061 atatattgga taatagcact gattatttta ataatcactc gtcggattat cagaggtagg
     9121 cttaataagt cacaaacacc gtatcctatg gtgttttcta ttggaggttc tcatgatgcg
     9181 gagtttaatg ctgaagggca tggttttaaa tggaaagcat atgcagatgt taaaaggaga
     9241 gatcgatcca cgaatgagcc tttagatatt caagtagata gagtaaaggg cccatattgt
     9301 atgaatgatt ttagagaaat gaaagtatct agaacttatt ggggcaggta taaatataaa
     9361 tgtcctaaat gtggatataa aagaattctc ctaaaaaatg cttggacgtt aaaatgtgat
     9421 atcggagatg aaattgaagc aggctatagg gataaagtta atacgagata attatagaat
     9481 gcacaaccta ccgttagttt aattagcagt aggttgtttt attatgtgag taaattttaa
     9541 ttaaacacgg ttatttttct gatgtttgct cttgagttct ggagttactc tgttttggga
     9601 ccaaatcaaa taaatgtgga ttttctttga tgaatttaat gattggatca aagaaacgtt
     9661 gttgttgctc tagtgtcata cctagtccgt ttaccattgg ataacatcct cctttaagtt
     9721 agatagcatt ccttcatgta aaaactatta gatggtatta aaaccactta gaattaaatc
     9781 tagagcggtt atttaatgtg tatttacaat attctttaac ataagcattt ccacctttat
     9841 tataattttt caaaatgtaa ccattagaag tgaaaatatc aatacaaaag taaaaagcca
     9901 tccgtaaaaa acgaatggct tgccgttcta ggatcgatgg tagggcggcg tatcctacat
     9961 ctctggtaac ttacctaacc aatcaaatgt ttgatcaaga acggcggcta gataagcgcg
    10021 tggggaacct ttcccacctc aaagatccta actattatta tgcaaacttt gatgtcttta
    10081 atacatctac ttccttgaag ttcatataat atctatactt attgtatcat atttttatga
    10141 attgtaaact ctaagttaac ctatgaggtc catgtatggc tgtagacgtc cactttttag
    10201 acgtttttcc actttaacag gcccgttgtt catgtcatac atagaagaca agaacaaata
    10261 accactcggg tctagtttaa tagcgattaa tatgtgttca tctataactt taattaattc
    10321 tacgctgtta ggttctttcg gattgtttcc tacgtaatcc ggattttcta tgatgtcttt
    10381 aatgttccct gaatattgtt cataaatacc aggatgtttt ctctgtatgt gctttatagc
    10441 accgggatac atttttacat cgttagaatc gcattctaat tcatatttat ctataatcac
    10501 ttgtggaagt gtacccacaa caattggttt atttacaatc gctttcttat cagccttcaa
    10561 atttctccct catctcgttt ctttatatct tcattttatc ataaaggagc atttgaactg
    10621 tcgcgcagga caggcttacg ggaaggatct tcatgctttg aaggtggaaa ttataaatat
    10681 atgcttgtcc actatatgtt tgatgtaatt aaaggataca tacccaccat aaccaaactt
    10741 cacttccaaa actcatatca tttccgttca ttacttacag ccaatatctc ttttacttct
    10801 tgtacttcac tgattgtttg cacgagcaat tcatatttta agcggcgctt taaagcctag
    10861 gtttttttag agaagaactt actgagaaat gatttgcttc tctcttcact ttgctggtga
    10921 acaaatttta acatttcatt catcttttct tgcatgtcag aaagttgttc cttcaattcg
    10981 tttttttctt gtctttcctt caatttctct tcacgttcat ctaaatacat ttttgttagg
    11041 ttctgattct gtaaatgtat atcctgttgc tgttcaatga gctgttgttg cttttcaaaa
    11101 acagcttgaa taacaagttg tttttcctca ttatctttat ggataacgtt taaaatctct
    11161 tgattttgta gagcgataat ttgttgctgt tttatcaatt cagccacttt ttcattggtt
    11221 tgttccaata tagcataagc gactccgaca gcactgttat tacgttgaag tggtgttatt
    11281 tcactaccat cactgtcgtt aatgctatgc gttagtgcat tgtcggtatc tttagaaaga
    11341 gatgattcta gcaatagaga tatagcttct tgtaaggttc tagttccgtc cgccattaaa
    11401 accttcatct ctttaaggat agtaatgtca tgttcgtaat agatgcgttg ttgacgatta
    11461 ttccgttcaa atttatatcc agcgctttct aactccaaac accaaacacg taatgtattt
    11521 ccgttcacat ctaattcttt gctaacttgt cccgaaaatc tcccgaagtc catcgaaatc
    11581 cctcctattc tcttgcatca taacggcatt attaatgcat tgtagcacca ttataacacc
    11641 gttaatgcga agtcactaat gccatttgct acttctatta agtctcctta actctgaggg
    11701 cacctcattg gtgaagattc acattcagtc ggaaaatgag aaacgaattc ccatccaggc
    11761 gaagggttac tactataagg gttacgatat aaagtaaaga gtgaaaataa cactaaaagg
    11821 tgttatgtta ttgctgtaa
//
