LOCUS       NZ_CP072609            10867 bp    DNA     circular CON 29-APR-2024
DEFINITION  Desulfovibrio desulfuricans strain L4 plasmid pDsulf-L4, complete
            sequence.
ACCESSION   NZ_CP072609
VERSION     NZ_CP072609.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17304468
            Assembly: GCF_017815575.1
KEYWORDS    RefSeq.
SOURCE      Desulfovibrio desulfuricans
  ORGANISM  Desulfovibrio desulfuricans
            Bacteria; Thermodesulfobacteriota; Desulfovibrionia;
            Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Karnachuk,O.V., Rusanov,I., Panova,I.A., Zyusman,V.S.,
            Grigoriev,M.A., Kadyrbaev,M., Pimenov,N.V., Gruzdev,E.V.,
            Beletsky,A.V., Mardanov,A.V. and Ravin,N.V.
  TITLE     Microbial sulfate reduction is an important source of hydrogen
            sulfide from a large swine finishing facility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   Karnachuk,O.V., Rusanov,I., Panova,I.A., Zyusman,V.S.,
            Grigoriev,M.A., Kadyrbaev,M., Pimenov,N.V., Gruzdev,E.V.,
            Beletsky,A.V., Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2021) Department of Molecular Biology of
            Microorganisms, Institute of Bioengineering, Research Center of
            Biotechnology of Ras, Prosp. 60-Let Oktyabrya, Bld 7-1, Moscow
            117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 302.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815575.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:22:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,959
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072609.1.
            Bacteria and source DNA available from Dr. Olga V. Karnachuk.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Desulfovibrio desulfuricans"
                     /mol_type="genomic DNA"
                     /strain="L4"
                     /db_xref="taxon:876"
                     /plasmid="pDsulf-L4"
                     /collection_date="2020"
     gene            join(10536..10867,1..508)
                     /locus_tag="J8J02_RS15060"
                     /old_locus_tag="J8J02_15060"
                     /db_xref="GeneID:72384065"
     CDS             join(10536..10867,1..508)
                     /locus_tag="J8J02_RS15060"
                     /old_locus_tag="J8J02_15060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /db_xref="GeneID:72384065"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            495..1346
                     /gene="repC"
                     /locus_tag="J8J02_RS15065"
                     /old_locus_tag="J8J02_15065"
                     /db_xref="GeneID:72384066"
     CDS             495..1346
                     /gene="repC"
                     /locus_tag="J8J02_RS15065"
                     /old_locus_tag="J8J02_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /db_xref="GeneID:72384066"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1654..2469
                     /gene="sul2"
                     /locus_tag="J8J02_RS15070"
                     /old_locus_tag="J8J02_15070"
                     /db_xref="GeneID:72384067"
     ARG             1655..2466
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2915..3333
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             2531..3330
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3333..4169
                     /locus_tag="J8J02_RS15080"
                     /old_locus_tag="J8J02_15080"
                     /db_xref="GeneID:72384069"
     CDS             3333..4169
                     /locus_tag="J8J02_RS15080"
                     /old_locus_tag="J8J02_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /db_xref="GeneID:72384069"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            4475..4717
                     /locus_tag="J8J02_RS15085"
                     /old_locus_tag="J8J02_15085"
                     /db_xref="GeneID:72384070"
     CDS             4475..4717
                     /locus_tag="J8J02_RS15085"
                     /old_locus_tag="J8J02_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /db_xref="GeneID:72384070"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4749..5399)
                     /gene="tetR(A)"
                     /locus_tag="J8J02_RS15090"
                     /old_locus_tag="J8J02_15090"
                     /db_xref="GeneID:72384071"
     CDS             complement(4749..5399)
                     /gene="tetR(A)"
                     /locus_tag="J8J02_RS15090"
                     /old_locus_tag="J8J02_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /db_xref="GeneID:72384071"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            5505..6704
                     /gene="tet(A)"
                     /locus_tag="J8J02_RS15095"
                     /old_locus_tag="J8J02_15095"
                     /db_xref="GeneID:72384072"
     ARG             5506..6701
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(<6671..6859)
                     /locus_tag="J8J02_RS15100"
                     /old_locus_tag="J8J02_15100"
                     /pseudo=""
                     /db_xref="GeneID:72384073"
     CDS             complement(<6671..6859)
                     /locus_tag="J8J02_RS15100"
                     /old_locus_tag="J8J02_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /db_xref="GeneID:72384073"
                     /name="IS91 family transposase"
     gene            complement(7413..7697)
                     /locus_tag="J8J02_RS15105"
                     /old_locus_tag="J8J02_15105"
                     /db_xref="GeneID:72384074"
     CDS             complement(7413..7697)
                     /locus_tag="J8J02_RS15105"
                     /old_locus_tag="J8J02_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /db_xref="GeneID:72384074"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            8001..>8489
                     /locus_tag="J8J02_RS15205"
                     /pseudo=""
                     /db_xref="GeneID:72384093"
     CDS             8001..>8489
                     /locus_tag="J8J02_RS15205"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /db_xref="GeneID:72384093"
                     /name="MobA/MobL family protein"
     gene            8644..9057
                     /locus_tag="J8J02_RS15210"
                     /db_xref="GeneID:72384094"
     CDS             8644..9057
                     /locus_tag="J8J02_RS15210"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /db_xref="GeneID:72384094"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            9156..10025
                     /locus_tag="J8J02_RS15215"
                     /db_xref="GeneID:72384095"
     CDS             9156..10025
                     /locus_tag="J8J02_RS15215"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /db_xref="GeneID:72384095"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            10086..10298
                     /locus_tag="J8J02_RS15115"
                     /old_locus_tag="J8J02_15115"
                     /db_xref="GeneID:72384075"
     CDS             10086..10298
                     /locus_tag="J8J02_RS15115"
                     /old_locus_tag="J8J02_15115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /db_xref="GeneID:72384075"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            10300..10506
                     /gene="urfF"
                     /locus_tag="J8J02_RS15120"
                     /old_locus_tag="J8J02_15120"
                     /db_xref="GeneID:72384076"
     CDS             10300..10506
                     /gene="urfF"
                     /locus_tag="J8J02_RS15120"
                     /old_locus_tag="J8J02_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /db_xref="GeneID:72384076"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             3334..4166
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7652..7807
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(2..514)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.805"
                     /coverage="82.0"
                     /evalue="0.0"
     Typing          10554..10867
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="2.1700000000000002e-163"
     Typing          7897..10022
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
       61 cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
      121 cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
      181 gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
      241 ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
      301 cctgtcgagc atgaccagcg ccgaggcaga ggaatggggt gtggacgacg accagcgccg
      361 gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
      421 gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
      481 caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
      541 acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
      601 gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
      661 cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
      721 ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
      781 tcctggaacc caagtgggag gccgtcaccg ctgatgccat ggtggtcaaa ggtagctatc
      841 gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
      901 aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
      961 aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
     1021 ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
     1081 tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
     1141 gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
     1201 ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg ccagcgggtg cgcgaggcgt
     1261 tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
     1321 tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
     1381 tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
     1441 tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
     1501 taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcctgc agctaatgga
     1561 tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc cgtatgagac
     1621 tcatgctcga ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc
     1681 atcgtcaaca taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca
     1741 gccattgcgc aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg
     1801 gcatccagca atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg
     1861 ccggtgctgg acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc
     1921 gcgacgcaag cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt
     1981 ccagacgctg cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat
     2041 tcggtgcaag acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac
     2101 attgcggcgt tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac
     2161 cgccttgtcc ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc
     2221 tcggtgctgg cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct
     2281 gtttcgcgca aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc
     2341 gcgacactcg ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac
     2401 gagccgcgcc ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga
     2461 attcgttaac tgcacattcg ggatatttct ctatattcgc gcttcatcag aaaactgaag
     2521 gaacctccat tgaatcgaac taatattttt tttggtgaat cgcattctga ctggttgcct
     2581 gtcagaggcg gagaatctgg tgattttgtt tttcgacgtg gtgacgggca tgccttcgcg
     2641 aaaatcgcac ctgcttcccg ccgcggtgag ctcgctggag agcgtgaccg cctcatttgg
     2701 ctcaaaggtc gaggtgtggc ttgccccgag gtcatcaact ggcaggagga acaggagggt
     2761 gcatgcttgg tgataacggc aattccggga gtaccggcgg ctgatctgtc tggagcggat
     2821 ttgctcaaag cgtggccgtc aatggggcag caacttggcg ctgttcacag cctatcggtt
     2881 gatcaatgtc cgtttgagcg caggctgtcg cgaatgttcg gacgcgccgt tgatgtggtg
     2941 tcccgcaatg ccgtcaatcc cgacttctta ccggacgagg acaagagtac gccgcagctc
     3001 gatcttttgg ctcgtgtcga acgagagcta ccggtgcggc tcgaccaaga gcgcaccgat
     3061 atggttgttt gccatggtga tccctgcatg ccgaacttca tggtggaccc taaaactctt
     3121 caatgcacgg gtctgatcga ccttgggcgg ctcggaacag cagatcgcta tgccgatttg
     3181 gcactcatga ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc agagcgcgcc
     3241 ttcgctgtcc tattcaatgt attggggatc gaagcccccg accgcgaacg ccttgccttc
     3301 tatctgcgat tggaccctct gacttggggt tgatgttcat gccgcctgtt tttcctgctc
     3361 attggcacgt ttcgcaacct gttctcattg cggacacctt ttccagcctc gtttggaaag
     3421 tttcattgcc agacgggact cctgcaatcg tcaagggatt gaaacctata gaagacattg
     3481 ctgatgaact gcgcggggcc gactatctgg tatggcgcaa tgggagggga gcagtccggt
     3541 tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc cggggagcga atgctctctc
     3601 acatcgttgc cgagcacggc gactaccagg cgaccgaaat tgcagcggaa ctaatggcga
     3661 agctgtatgc cgcatctgag gaacccctgc cttctgccct tctcccgatc cgggatcgct
     3721 ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa actgactacg
     3781 tccacgcggc gattatagcc gatcaaatga tgagcaatgc ctcggaactg cgtgggctac
     3841 atggcgatct gcatcatgaa aacatcatgt tctccagtcg cggctggctg gtgatagatc
     3901 ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac gatccggctg
     3961 acagagacga cctttgtctc gatcctagac gcattgcaca gatggcggac gcattctctc
     4021 gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat gggtgccttt
     4081 ccgcagcttg gaacgcggat ggagaagagg agcaacgcga tctagctatc gcggccgcga
     4141 tcaagcaggt gcgacagacg tcatactaga tatcaagcga cttctcctat cccctgggaa
     4201 cacatcaatc tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt
     4261 cccgcaaacg acccctggaa gccccgtagc gacgcggaga ggggcgagac aagccaaggg
     4321 cgcaggctcg atgcgcagca cgacatagcc ggttctcgca aggacgagaa tttccctgcg
     4381 gtgcccctca agtgtcaatg aaagtttcca acgcgagcca ttcgcgagag ccttgagtcc
     4441 acgctagatc tatctcatct gcgcaaggca gaacgtgaag acggccgccc tggacctcgc
     4501 ccgcgagcgc caggcgcacg aggccggcgc gcggacccgc gccacggccc acgagcggac
     4561 gccgcagcag gagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac
     4621 gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa
     4681 agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg ctccggcagc
     4741 ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt
     4801 ttcgccaatc catcgacaat cactgcgagt ccctgctcga acgctgcgtc cggaccggct
     4861 tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg
     4921 ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc cccaacagtg
     4981 aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag
     5041 aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca
     5101 tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg
     5161 atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc
     5221 agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc
     5281 gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg
     5341 acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg
     5401 cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg ataaagaatc
     5461 cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa cccaacagac
     5521 ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc
     5581 cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg
     5641 gcattctgct ggcgctgtat gcgttgatgc aatttgcctg cgcacctgtg ctgggcgcgc
     5701 tgtcggatcg tttcgggcgg cggccggtct tgctcgtctc gctggccggc gctgctgtcg
     5761 actacgccat catggcgacg gcgtctttcc tttgggttct ctatatcggg cggatcgtgg
     5821 ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat atcactgatg
     5881 gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc gggatggtcg
     5941 cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg
     6001 cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg gagtcgcaca
     6061 aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg
     6121 cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg caacttgtcg
     6181 gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac tgggacgcga
     6241 ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc caggcaatga
     6301 tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc ggaatgattg
     6361 ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg gcgttcccga
     6421 tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca atgttgtcca
     6481 ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg ctcaccagcc
     6541 tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct ataacaacgt
     6601 ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg ccggcgctgc
     6661 gtcgcgggct ttggagaaat tcttcaaatt cccgttgcac atagcccggc aattcctttc
     6721 cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
     6781 gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
     6841 tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
     6901 gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
     6961 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
     7021 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
     7081 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
     7141 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
     7201 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
     7261 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
     7321 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
     7381 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
     7441 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
     7501 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
     7561 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg cacccgctga
     7621 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
     7681 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
     7741 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
     7801 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
     7861 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
     7921 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
     7981 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
     8041 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
     8101 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
     8161 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
     8221 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
     8281 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
     8341 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
     8401 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
     8461 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
     8521 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
     8581 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
     8641 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
     8701 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
     8761 cagacccagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
     8821 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
     8881 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
     8941 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
     9001 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
     9061 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
     9121 atatcggcgt cagggacgcc accaccggcc agatgatgaa ccgggaatgg tcagccgccg
     9181 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
     9241 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
     9301 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
     9361 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
     9421 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
     9481 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
     9541 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
     9601 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
     9661 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
     9721 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tgatgacctc agcaagtgcg
     9781 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
     9841 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
     9901 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
     9961 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
    10021 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
    10081 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
    10141 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
    10201 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
    10261 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
    10321 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
    10381 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
    10441 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
    10501 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
    10561 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
    10621 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
    10681 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
    10741 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
    10801 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
    10861 agccgct
//
