LOCUS       NZ_CP072595             1933 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB976 plasmid pVB976_p9, complete
            sequence.
ACCESSION   NZ_CP072595
VERSION     NZ_CP072595.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533868
            Assembly: GCF_017815695.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB976
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB976
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.4x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815695.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,208
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 2,986
            CDSs (with protein)               :: 2,986
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 41 of 131
            Pseudo Genes (incomplete)         :: 81 of 131
            Pseudo Genes (internal stop)      :: 34 of 131
            Pseudo Genes (multiple problems)  :: 22 of 131
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072595.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB976"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB976_p9"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            190..1155
                     /locus_tag="J7300_RS15900"
                     /old_locus_tag="J7300_15940"
     CDS             190..1155
                     /locus_tag="J7300_RS15900"
                     /old_locus_tag="J7300_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_044384225.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVDDKVHPLKLSWSIDRITIVGK
                     LKENIYYHTPNDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLEMQLRRG
                     KATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLL
                     KEESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1355..>1537
                     /locus_tag="J7300_RS15905"
                     /old_locus_tag="J7300_15945"
                     /pseudo=""
     CDS             1355..>1537
                     /locus_tag="J7300_RS15905"
                     /old_locus_tag="J7300_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /name="recombinase"
     Typing          274..1152
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.206"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 agcagtgttg acgcactctc gacctttacg gaaactaatc acactagctc cgtaacttct
       61 taatcattat acgaatattt ttaggtttct tcaagactag agattttttg gtaatgaatt
      121 tttaagagtt acgtgaactt ttgaaaattc tttccagaag tctctttttt ctttgaggag
      181 gaactaaatt tgagtaaaaa aggcacgatc ataaatgagt gggaaaaatt aacagccccc
      241 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
      301 gatcgaatta caatcgtcgg aaaactgaaa gaaaatattt attatcacac tccaaatgat
      361 gtgttgattt tagattttga acagcttatg cgcctaaatg aaggtaacgg ttatttaaga
      421 tctgttagta ataatggttg gcagttgtta gaccaacatg aagagaatat tgcttatatt
      481 gaaattttaa aatggcaaga ggggaaaggg cgtatagact tcaatccgaa taagattagt
      541 caattcttag ctagttccat gaaaaatttt attcatgatt tgtttatcga acctcacttt
      601 tcacgtgcag atattgcttg cgacattatc gatgtacctg atgagtttat tacacaatat
      661 cgggtagttg atccagtttc ttttaagcct atttatggac ggaatggaaa attagagacg
      721 gcatattggg gatctcgctc ttctgaacgt caaattcgga tgtataataa aaaactggaa
      781 caagagaaga aaagaaaaat tgtgccaaaa gaaatcgtga gttggtggcg gttagaaatg
      841 cagctaagac gtggcaaagc aacagattgg catgcgatgg ttcgggaaag tttagatagc
      901 tttgcaagtc ctcattactt gcctggagat gtaaaacctg ttgatagaat tatgataaaa
      961 gggctgaatc aagatcattc agagtgggcg tatattagca gaaatttgaa atatagattg
     1021 cgcaaattgt tgaaagaaga aagccaaaat gatgaattga cgaatcattt gcgagaaacc
     1081 tttgcagaat cggcagacga tttaaaaaaa gaattagaca catggcttct agggctagac
     1141 gtaacagagg agtgataccg aataagaaaa gtcagatgag tgagagtaaa tttacgtcaa
     1201 tgtttaatga cgtgagttta cgataagcga ttattttgcg tgaagcaaaa taaagcgtaa
     1261 aaggagttga gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacagcga
     1321 ataccctcgg agagcatttg gaattgtgac agccatgtca caattaggaa aatattactc
     1381 tttgtcaaag agtaacagaa aggtttacac tctttttatt ggcgacagcg cgaaggaggg
     1441 gtttaacttg gggtttagta tttcgttcaa aaagggaaca aaaaatacaa gcatttctca
     1501 taataatcgt gagttaactg atagccaaaa aatcaatggt ttgtctggta gaaaataatt
     1561 agcagattta caagtgggaa ataaaaagct gaaagaagaa aattgcattt caaactacat
     1621 tttctacaat agctctaaaa tggcatattt tgcgttctga gcggttttag cgtttatttc
     1681 gtttagttat cggcacaatc ggtgaaacac gttagaggag cgaaaatgga gcctgtaagg
     1741 cgactggtag cggaacggtc gtgttagatt gtgtagccga tagctgaacg aaataaatgc
     1801 tggcagcgcc attctatttc ggcaggagga ggctcaaggg gttcggggaa tgaaattccc
     1861 tgaggtcttt ttattttttc tagaggagtg ctataatcaa agaaaatgag aaaaaagagt
     1921 aaaaaaatag tcg
//
