LOCUS       NZ_CP072594             1979 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB976 plasmid pVB976_p8, complete
            sequence.
ACCESSION   NZ_CP072594
VERSION     NZ_CP072594.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533868
            Assembly: GCF_017815695.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB976
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB976
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.4x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815695.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,208
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 2,986
            CDSs (with protein)               :: 2,986
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 41 of 131
            Pseudo Genes (incomplete)         :: 81 of 131
            Pseudo Genes (internal stop)      :: 34 of 131
            Pseudo Genes (multiple problems)  :: 22 of 131
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072594.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB976"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB976_p8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            178..1194
                     /locus_tag="J7300_RS15890"
                     /old_locus_tag="J7300_15930"
     CDS             178..1194
                     /locus_tag="J7300_RS15890"
                     /old_locus_tag="J7300_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002299382.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGTT
                     TPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQLF
                     DQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIACDI
                     VDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERKKVIV
                     PKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGLIAEPS
                     NWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1394..>1576
                     /locus_tag="J7300_RS15895"
                     /old_locus_tag="J7300_15935"
                     /pseudo=""
     CDS             1394..>1576
                     /locus_tag="J7300_RS15895"
                     /old_locus_tag="J7300_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          179..1188
                     /name="rep_cluster_935"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.292"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgcgctaaca ctctcgacta tttacggaaa ctacttcacc tagctccgta actcaggttc
       61 attatacgaa tatttttagg tttcttcaag ctagagattt ttggatatga atttttaggg
      121 ttacgtgaac tctggaaatt ctttccaaaa gtctcttttt tcgtttggag gacgaaaatg
      181 gcaaatgaaa atcagttgag tgaaattcaa tcaaaaattg aaggtcgagg ggcagaaaaa
      241 agaacaacga taaatcagct cgaaaaactt aaccgtattt ctaacaggcg gttaagtgtg
      301 caaggtacga ccacccccct aaaattaaat tggtcgattg atcgaattac aattgtcgga
      361 aaactgaatg ataccatagc ttattggacg aaaaatggag ttgtttttgt cgatttcgag
      421 caattaatgc gtttgaacga aaatcaggga tatttgaaat cagttgggat aaacggctgg
      481 caattattcg atcaatatgg agaagatatt gcgtatatcg aaatacttaa atttcatgaa
      541 ggaaaaggtc gtattgattt taatccgaat aagataggcc agtttttagc tagttccatg
      601 aaaggattca ttcatgacct atttttagat ccgcattttt cccgggcgga tattgcctgc
      661 gatattgtag atgtgccgga tgactttatt actcagtatc gtattgtaga cccgatttct
      721 tttaagccaa tctacggccg ttctggcaag ctagaaacag cgtactgggg cagtcgtgcc
      781 agtgagcgcc aagtgcgact gtacaacaaa aagctagagc aggaacgaaa aaaagtaatc
      841 gtgccaaaag aaatcgatac gtggtggcgg ttagaaatgc agctaagacg tggcaaagca
      901 acggattggc atgcgatggt tcaggaaagc ttagatagtt ttgcaagccc gcattttttg
      961 ccgattgata taaaacctat tgataaaata gttattgatg gtttgatagc tgagccttct
     1021 aattggtcga taattgctag acatacaaaa tataaatatc ggaatttgct gaagcaagaa
     1081 agtcaaaatg atgagttaac gaatcattta cgagagactt ttaaggaatc tgcagacgaa
     1141 ttaaaaaaag aattggatac gtggcttttg gggttggatg tgacagagaa gtgataccga
     1201 ataagaaaag tcagatgagt gagagtaaat ttacgtcaat gtttagtgac gtggatttac
     1261 gataagcgat tattttgcgt gaagcaaaat aaagcgtaaa aggagttgag agcgtgacac
     1321 gctttcggct ccttttttcg ttgtcacttg tgacagcgaa taccctcgga gagcatttgg
     1381 aattgtgaca gccatgtcac aattaggaaa atgttactct ttgtcaaaga gtaacagaaa
     1441 ggtttacact ctttttatta gcgacagcgt gaaggagggg ttcaccttag ggtttagtat
     1501 ttcgttcaaa aagggaacaa agaatacaag catttctcat aataatcgtg agttaactga
     1561 tagccaaaaa atcaatggtt tgtctggtag aaaataatta gcagatttac aagtgggaaa
     1621 taaaaagctg aaagaagaaa attgcatttc aaactacatt ttctacaata gctctaaaat
     1681 ggcatatttt gcgttctgag cggttttagc gtttatttcg tttagttatc ggcacaatcg
     1741 ttaaaacacg ttagaggagc gaaaatggag cctgtaaggc gactggtagc ggaacgatcg
     1801 tgttagattg tgaaaaccga tagctgaatg aaataaacag cgggtgcgcc ctttccattt
     1861 cggcaggagg aggctcaagg ggttcgggga atgaaattcc ctgaggtctt tttatttttt
     1921 taggaggagt gctataatca aagaaaacga gaaaaaagag caaaataaaa gccgagcag
//
