LOCUS       NZ_CP072593             2057 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB976 plasmid pVB976_p7, complete
            sequence.
ACCESSION   NZ_CP072593
VERSION     NZ_CP072593.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533868
            Assembly: GCF_017815695.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB976
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB976
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.4x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815695.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,208
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 2,986
            CDSs (with protein)               :: 2,986
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 41 of 131
            Pseudo Genes (incomplete)         :: 81 of 131
            Pseudo Genes (internal stop)      :: 34 of 131
            Pseudo Genes (multiple problems)  :: 22 of 131
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072593.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB976"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB976_p7"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            100..255
                     /locus_tag="J7300_RS15870"
                     /old_locus_tag="J7300_15910"
     CDS             100..255
                     /locus_tag="J7300_RS15870"
                     /old_locus_tag="J7300_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            322..924
                     /locus_tag="J7300_RS15875"
                     /old_locus_tag="J7300_15915"
     CDS             322..924
                     /locus_tag="J7300_RS15875"
                     /old_locus_tag="J7300_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1060..1317)
                     /locus_tag="J7300_RS15880"
                     /old_locus_tag="J7300_15920"
     CDS             complement(1060..1317)
                     /locus_tag="J7300_RS15880"
                     /old_locus_tag="J7300_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1318..1539)
                     /locus_tag="J7300_RS15885"
                     /old_locus_tag="J7300_15925"
     CDS             complement(1318..1539)
                     /locus_tag="J7300_RS15885"
                     /old_locus_tag="J7300_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          complement(983..1158)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          323..852
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 taaggcctat tattcgctaa ttagtatcta aactgttgca ccttagttac ttttttgtta
       61 ctatggtttt taagaaaaag tcgttggagg atacgaatta tgatcagtaa tgaaaaaaag
      121 agagtcgttg taacgcttac taaggaagtg gcagataatc ttgataaaat tgcgaaacaa
      181 atgggtctta gtaagtctgg tcttattaca atttgggtta atgctaacgg aaaggaaaat
      241 gagcaaaaaa aatagcaaaa gcattagtgt tggcgcactg cttttgctgt tagactgttg
      301 ttcaattaag gtaattatag catgacgagt agaaaaacta aagatgaacg cacaaggaat
      361 tggacgtttg tagtctatcc tgaatcggct cctgaaaatt ggagggagtt tttagatgag
      421 ttacatgttc cttgggttga gagtccactt catgataaag atgtgaatcc tgacggtgaa
      481 ttgaaaaagc ctcattggca tgttttgata atgtttagcg gtaaaaagac gtatgatcaa
      541 atacgtgaga taactcaaaa gcttcggtct ccaaacccgc aaaaatgtgc taatgcaaaa
      601 ggtatggtgc gatattttgc tcatatggat aatccagaaa aatttcaata tgctaaaagt
      661 gatattatcg ctcatggtgg tgcagaaatt gctagttatt tgtcagtaac gagtgcagaa
      721 cgatatgagt tgattagaga gatgatgtcg tttgttgatt ctaagaatat cactgaaatc
      781 aaagatttaa ttgactatgc aatgtcagaa agatttgatg attggtttcc gcttctttgt
      841 gataattcag cttatattat cgggcaatat ataaaatcga atcgtcatgg tggaagtgta
      901 aattcaaaaa taaataaagg ataagagctg ttaatttcag ctcttttttt gtgcgtgaaa
      961 gtcttagtca ctatgaccaa aactacggac gaaactatga ccaaaagtag gtactatatt
     1021 atgaccgaaa ataggtccaa aatggatttt tttgtgatat caaaaaatat aaggttgttt
     1081 tgtaagaaaa ccagttggca cagccgactt agccaccttc ctgtaggaag gtgctatcag
     1141 ttttccctta tgctcttttt gttcttcaga gtttaattcc agaatatttg tgagaagtag
     1201 gtcaaagagg tcggtggttt cttcgacttt tccgccggtc aattcgataa aatcattgga
     1261 atatgattct agcaattttt ctagttgttc aacgttgata gttgtttctt taggcattta
     1321 ctcaccttct ttggtggtta tgactttttc tagtttttta agggcttctg ggctgaaaag
     1381 atagtttctt ctaccggcag agcgatattc ttttccttca gtcagatgtt ctttcgctaa
     1441 gcgcaaaacg taagcttggt tgtaatcaag ttcttgtgca agttcgctgg tcgtcataat
     1501 ttcggccaaa gtgaattttt ctgattttat cactctcatt tatgctcctt ccatttaacg
     1561 tccgtctata ttacgtgagt acgtcggact tacgacataa gcttataata taagttttct
     1621 gtgttgtggt caattttata ttatcgaaat gactagtaga aaaaatcaat gctctaaaaa
     1681 gggtctattt tgcgttttaa gcgatttagc gtctatttcg tttagttatc ggcgcaatcg
     1741 ttaaaacacg taagagaagc gagaatggag cctgtaaggc gactggtagc gaaacggtcg
     1801 tgttagattg caaaagccga tagctgaacg aaatagtaag cgtggcgcct gcatatattt
     1861 cggtagaagg aggtcggggt tcggggcgga gccctgaggt ttgttttttg gttttggtgt
     1921 ttatgaattg ggcggaggag gggggggact acgacacccc ccttattggt cattggtcat
     1981 tggtcgatag ctgtttagcc ttactctccc aagggtttca gcgttttttt ttgacaaaat
     2041 cagtaatgcc aagggtt
//
