LOCUS       NZ_CP072591             4466 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB976 plasmid pVB976_p5, complete
            sequence.
ACCESSION   NZ_CP072591
VERSION     NZ_CP072591.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533868
            Assembly: GCF_017815695.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB976
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB976
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.4x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815695.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,208
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 2,986
            CDSs (with protein)               :: 2,986
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 41 of 131
            Pseudo Genes (incomplete)         :: 81 of 131
            Pseudo Genes (internal stop)      :: 34 of 131
            Pseudo Genes (multiple problems)  :: 22 of 131
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072591.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB976"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB976_p5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            join(4237..4466,1..694)
                     /locus_tag="J7300_RS15820"
                     /old_locus_tag="J7300_15860"
     CDS             join(4237..4466,1..694)
                     /locus_tag="J7300_RS15820"
                     /old_locus_tag="J7300_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            710..1249
                     /locus_tag="J7300_RS15825"
                     /old_locus_tag="J7300_15865"
     CDS             710..1249
                     /locus_tag="J7300_RS15825"
                     /old_locus_tag="J7300_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            1934..2107
                     /locus_tag="J7300_RS15830"
                     /old_locus_tag="J7300_15870"
     CDS             1934..2107
                     /locus_tag="J7300_RS15830"
                     /old_locus_tag="J7300_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            2513..2947
                     /gene="bacA"
                     /locus_tag="J7300_RS15835"
                     /old_locus_tag="J7300_15875"
     CDS             2513..2947
                     /gene="bacA"
                     /locus_tag="J7300_RS15835"
                     /old_locus_tag="J7300_15875"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     Typing          2..694
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca caaaatatag
       61 cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta aaggactttt
      121 taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt tggatagcac
      181 atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct ggactgaatt
      241 ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga atagtccgtc
      301 aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct tatttaaagg
      361 attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg gttatcgttt
      421 tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac aagaagattt
      481 acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag aaaaatggcg
      541 cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt ttaaacaaca
      601 acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac tcaaacaaga
      661 tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta tggcagataa
      721 aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta acaaaaaagt
      781 ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg tgattaacga
      841 aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact taaatcgaaa
      901 actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag tttccgaatt
      961 aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc tacaagaaaa
     1021 agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac tcactctaca
     1081 agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata cagaagaaac
     1141 tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac aaccacttat
     1201 tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa attttctaga
     1261 agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc actcaaattt
     1321 ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag ttgtaaacta
     1381 tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa tttttggtgg
     1441 aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac aaatgtgatt
     1501 ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag aattttgatc
     1561 atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa taaagtatgg
     1621 gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc ttgaccattt
     1681 tctctcaacg tggtaaaaat cccacttata agagaaaatg gtcaagaacc taaacggtcg
     1741 gcgaagaaaa cataccaagc accacaagca aaaaaagtta gagtaacttc caaaaaagaa
     1801 aaatttttta gaacaacttt tgaaaattta attttgattt atatccgttc aatttaaaca
     1861 tttgataaac tgaaggctag tatttaaaat gtgtatacaa gtggtagcgt taaaaaaagt
     1921 ggaggtataa cctatgttaa aaaaaactgt gttggtactg ggttctattt ctctaggaat
     1981 attaggtttt agccccatgc tattatccgt aattggaaaa aacaattcca acggagaact
     2041 taaattaaat tcaaattatt acaacagtcc taacgaagaa ttgtttgtaa aagagacgga
     2101 taaataataa aaaagactgc tctttcgagc agccagttaa aaaaataatg agagtttatt
     2161 cttgaaaaag caacgcgctt ataaagcgct accaaaattt tatcatattt tataaaaaat
     2221 aacaatccag taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaactaca
     2281 gaaaatcaag ccttaaaaag agctttttac ctagaccaat taaaaatcac aaagtgagcg
     2341 tctctatcca ttggtagagg ggcaacttgg tctagtcgac attatgtcta ctaaccttta
     2401 aaataacacg ttttataaaa taaaccctct tgacaatatt taattttctg attattctaa
     2461 atatatacag aaagcgcttt cctaggattt tatatctaag gaggaagtaa caatgaaaaa
     2521 taaaattatt aaaattatta cttgtctatt tttgttcggt atgattgttg gagcaagtac
     2581 tccgttaaat ctgtttcctt ttagtgctag aggtataaaa gtggaagctg caagttcacg
     2641 atataatcat aatcatcgtg gatttacttg ctataaaatg aactattatg ttactaataa
     2701 aatgtgtaga gatttgaaaa aaaattataa taagctaaag actcctgctc aaattgcatc
     2761 ttttattcct attggtggag ttggaacatg ggttattaca aatgctttta gtagtgctgt
     2821 tgatgcaatg aatgtctttg taagagctgc aaatcaagga aaaggtgtac aattaacata
     2881 taatgctcac tttagcaata caacatcata tcaatacaat gattacgctc gttatgtaat
     2941 aaaataatca gaaagtgaaa ctatctcatg aataaaaaga ttttagctgt aattatttca
     3001 ataatagttt taatcactat gttcttcata tttaggctaa ttttcaatat aagcttacaa
     3061 aaaagtattc tttatctaat tcctatagca tttatcttag caatttttag aagtatttac
     3121 ggaaataaaa actctaaata gctaaaaata aatcatacta aattttacaa aaagtggcta
     3181 gcattttcta gccacttttt gtattgtgga gcgtgggttt tgagggggat tcaaggggga
     3241 gcttgcgccc ctttgacaag cggttatttg caccaaatac ggaggtattt ggctagcaaa
     3301 tatagagctt gccaaaccct tttagtgagt tttgtagaaa gtacctttaa aggtactttc
     3361 acattacgaa cgatacgtcc gtaggacgta ctgaaagggt gggacttatg agcaaattaa
     3421 tgcaaaattt ggctagcaaa aaagctaaaa aatatatgct aataatcgag cgaaataaac
     3481 actttttcaa gtgagcgaat ccgccatgac aaagctgaag cagtttattt cagctttgtc
     3541 atggcgtgtg ccgacttttt taaaagtcta ggcacacgag caaatatctt tatcaagctc
     3601 taatttgcgt tttaagacgt ttgaaacgcc taagtatata aatatataag aataggttta
     3661 agagtgctta aatcgaattt tagagggcat ttagacaatt ttagggttgt tagggcttgc
     3721 cctgaccgcc tgtaaggcgt tttaagaaca agataaggga tttatactag gactgtctta
     3781 aaatgtctta aacgaacgct cagagcttct caaagggttc cttatgaacg gcttttagca
     3841 aaggagccgg aggcgactgc gcagtataat gtgagagcag agcgatcaca cctttttagc
     3901 gaaccgtagg gagcaaggtt tggaattggc agttttgata aattcgcgaa cctgtttaga
     3961 gaaaaaagga gtgatttttt aggcgggaaa attagcctcc cctcttttta tatctctttt
     4021 tatatctctt tttatatctc ttttatgtcg gctgtgtcct tactctccca agcaattcag
     4081 agttttttca agtaggtttt ctacgctttt caagtaggtt ttctacgctt ttcaagtagg
     4141 ttttctacgc ttttcaagta ggttttccga cattattgag tatttttata aagtatgtta
     4201 cttttatttc atactttata aaagtaggag aagcttatgg caaatgaaat tgtaaaatat
     4261 gaaaatagat taaatcatat tcctctgaga aaatttaata gtcgtgaaat gaacttattt
     4321 ttttcaattg cttcacgtgt acgtgaaaaa ggtgtagatg agattatttt ttcatttcac
     4381 gatttgaaaa aactaagcgg ttatacagaa cgtggggaac aatttataaa agatcttagt
     4441 agcacgtatg acaaattatt aagtat
//
