LOCUS       NZ_CP072585             2056 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB12993 plasmid pVB12993_p6, complete
            sequence.
ACCESSION   NZ_CP072585
VERSION     NZ_CP072585.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533867
            Assembly: GCF_017815655.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB12993
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB12993
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.7x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815655.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:39:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 37 of 144
            Pseudo Genes (incomplete)         :: 94 of 144
            Pseudo Genes (internal stop)      :: 39 of 144
            Pseudo Genes (multiple problems)  :: 23 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072585.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB12993"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB12993_p6"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            join(2049..2056,1..250)
                     /locus_tag="J7299_RS15570"
                     /old_locus_tag="J7299_15570"
     CDS             join(2049..2056,1..250)
                     /locus_tag="J7299_RS15570"
                     /old_locus_tag="J7299_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(386..988)
                     /locus_tag="J7299_RS15575"
                     /old_locus_tag="J7299_15575"
     CDS             complement(386..988)
                     /locus_tag="J7299_RS15575"
                     /old_locus_tag="J7299_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1055..1210)
                     /locus_tag="J7299_RS15580"
                     /old_locus_tag="J7299_15580"
     CDS             complement(1055..1210)
                     /locus_tag="J7299_RS15580"
                     /old_locus_tag="J7299_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1827..2048
                     /locus_tag="J7299_RS15585"
                     /old_locus_tag="J7299_15585"
     CDS             1827..2048
                     /locus_tag="J7299_RS15585"
                     /old_locus_tag="J7299_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          153..328
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(459..988)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agaaacaact atcaacgttg aacaactaga aaaattgcta gaatcatatt ccaatgattt
       61 tatcgaattg accggcggaa aagtcgaaga aaccaccgac ctctttgacc tacttctcac
      121 aaatattctg gaattaaact ctgaagaaca aaaagagcat aagggaaaac tgatagcacc
      181 ttcctacagg aaggtggcta agtcggctgt gccaactggt tttcttacaa aacaacctta
      241 tattttttga tatcacaaaa aaatccattt tggacctatt ttcggtcata atatagtacc
      301 tacttttggt catagtttcg tccgtagttt tggtcatagt gactaagact ttcacgcaca
      361 aaaaaagagc tgaaattaac agctcttatc ctttatttat ttttgaattt acacttccac
      421 catgacgatt cgattttata tattgcccga taatataagc tgaattatca caaagaagcg
      481 gaaaccaatc atcaaatctt tctgacattg catagtcaat taaatctttg atttcagtga
      541 tattcttaga atcaacaaac gacatcatct ctctaatcaa ctcatatcgt tctgcactcg
      601 ttactgacaa ataactagca atttctgcac caccatgagc gataatatca cttttagcat
      661 attgaaattt ttctggatta tccatatgag caaaatatcg caccatacct tttgcattag
      721 cacatttttg cgggtttgga gaccgaagct tttgagttat ctcacgtatt tgatcatacg
      781 tctttttacc gctaaacatt atcaaaacat gccaatgagg ctttttcaat tcaccgtcag
      841 gattcacatc tttatcatga agtggactct caacccaagg aacatgtaac tcatctaaaa
      901 actccctcca attttcagga gccgattcag gatagactac aaacgtccaa ttccttgtgc
      961 gttcatcttt agtttttcta ctcgtcatgc tataattacc ttaattgaac aacagtctaa
     1021 cagcaaaagc agtgcgccaa cactaatgct tttgctattt tttttgctca ttttcctttc
     1081 cgttagcatt aacccaaatt gtaataagac cagacttact aagacccatt tgtttcgcaa
     1141 ttttatcaag attatctgcc acttccttag taagcgttac aacgactctc tttttttcat
     1201 tactgatcat aattcgtatc ctccaacgac tttttcttaa aaaccatagt aacaaaaaag
     1261 taactaaggt gcaacagttt agatactaat tagcgaataa taggccttaa acccttggca
     1321 ttactgattt tgtcaaaaaa aaacgctgaa acccttggga gagtaaggct aaacagctat
     1381 cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct ccgcccaatt
     1441 cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc gacctccttc
     1501 taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc ttttgcaatc
     1561 taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc tcttacgtgt
     1621 tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa acgcaaaata
     1681 gacccttttt agagcattga ttttttctac tagtcatttc gataatataa aattgaccac
     1741 aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca cgtaatatag
     1801 acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa ttcactttgg
     1861 ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa gcttacgttt
     1921 tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc ggtagaagaa
     1981 actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc accaaagaag
     2041 gtgagtaaat gcctaa
//
