LOCUS       NZ_CP072576             3866 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB3895 plasmid pVB3895_p9, complete
            sequence.
ACCESSION   NZ_CP072576
VERSION     NZ_CP072576.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533866
            Assembly: GCF_017815675.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB3895
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB3895
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44.3x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815675.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:33:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 35 of 104
            Pseudo Genes (incomplete)         :: 70 of 104
            Pseudo Genes (internal stop)      :: 25 of 104
            Pseudo Genes (multiple problems)  :: 20 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072576.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3895"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB3895_p9"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            complement(join(3106..3866,1..220))
                     /locus_tag="J7291_RS15130"
                     /old_locus_tag="J7291_15140"
     CDS             complement(join(3106..3866,1..220))
                     /locus_tag="J7291_RS15130"
                     /old_locus_tag="J7291_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355134.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_002299242.1"
                     /translation="MAKKKQDLINGTFREKKRNLKKIMAFGDGRISGKTLENMKACGSF
                     MEFIATVDKEKKKMVHGMFCKNRFCPVCAYRKARKDSYMLSIMMEAIQAEKGYEYIFMT
                     LTTPNVPADKLSEEIDRFNKALSKLFRRKRVMTSIKGYVRKLEITYNKSRNDYNPHFHL
                     IMAVNKSYFTDKRYYIAQAEWLDMWRDVTGLDGINEDGTDEICSLDVRKVNGYRQEKAV
                     SEIAKYSAKDFEMTYSQDVFDTFYFAMKGRQLMTFNGVFKEYRKKYDAGELDHYRQKDE
                     NDYFWFLTASWMQKDANYSVAVRELTDDEKKFFAERYDKEGDETG"
                     /name="protein rep"
     gene            complement(828..2234)
                     /locus_tag="J7291_RS15135"
                     /old_locus_tag="J7291_15145"
     CDS             complement(828..2234)
                     /locus_tag="J7291_RS15135"
                     /old_locus_tag="J7291_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_127837500.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYIYRQREENEREK
                     ALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(2456..2818)
                     /locus_tag="J7291_RS15140"
                     /old_locus_tag="J7291_15150"
     CDS             complement(2456..2818)
                     /locus_tag="J7291_RS15140"
                     /old_locus_tag="J7291_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098379973.1"
                     /translation="MGVTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNKSQ
                     MSESKFTSMFSDVDLR"
                     /name="hypothetical protein"
     gene            complement(2871..3119)
                     /locus_tag="J7291_RS15145"
                     /old_locus_tag="J7291_15155"
     CDS             complement(2871..3119)
                     /locus_tag="J7291_RS15145"
                     /old_locus_tag="J7291_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323248.1"
                     /translation="MKLDKQRLYEAVKDNCNCGGNRQLFKAMMVTVYRRLDGENAETAV
                     ENAYFQVFGEHHSPEWDRAILDDMAEMHEKLWRALND"
                     /name="hypothetical protein"
     Typing          complement(3107..3866)
                     /name="rep_cluster_1077"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.802"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(832..2234)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.735"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacaaaccgg acaaaaccgg tttttacaga acatgccatg aaccatcttt ttcttctcct
       61 tatcaaccgt tgctatgaac tccatgaatg atccacaggc tttcatgttt tccaaggttt
      121 tgccagaaat acgaccatca ccaaatgcca tgattttctt taaatttctc tttttttctc
      181 ggaaagtgcc attgatcaaa tcttgctttt tcttcgccat atgctatact gaaccttgca
      241 gaacacaaac gttccgccca aacatttaaa aaacgcattt tctaaatgca cgaaaaaaag
      301 gttgcactcc tctcttgttt tatgactatt gacttagaca ctcaaagcat acaacactag
      361 gtacggatat gcaacttttt tttgtttaaa acccttatag gtcaagggaa aaacacatac
      421 ataggacccg attttctgcc gaaaatagtt accctatgta tcaagataag aaaaaacatc
      481 aaaaaaagag ggcgaaaatc gccccctttt tttcgtttta aaaccccaaa acccaaaggc
      541 ataaatttcg gtcaattgac cactgaattt ttaggggggt gtccataaaa gggacactta
      601 accgcctgtt agagggggct gttaattttt cccactcatt cttagtcatg caaacataaa
      661 accgcaccct ttaaaaaggg cgcccccaaa cttcgctaac gctaagttgg ccaaaaaatg
      721 gacttgccat tttttagcaa atgtcagctt cagggattag tggtttagcc gtcaactcct
      781 tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta ccactcgatc
      841 tcatcttcag aacgctctaa tttatgctgt gagacgtttt gacgataaaa taaggttttc
      901 tcctgctcta atacttctaa ttcctcagaa agccccatat ccttttctgc aagcccaaaa
      961 cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc taaacgctgg
     1021 cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc tgctaattgt
     1081 tttctaccag acaaatgacg ctcttcttcc aatgcagcta catactcatt taattctgta
     1141 ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct cttggcattt
     1201 gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa atgatacgaa
     1261 ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa aatgaccgcc
     1321 atctcttttt ttacattcag catatttttc ttctcttctt caatctccgc tctctcagaa
     1381 gccaccagag ccttctcacg ctcgttttct tctctttgcc tataaatata cctcatttcc
     1441 gcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat ttcactcatg
     1501 atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc ccttttccaa
     1561 ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa attattgaaa
     1621 agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc tttattaaag
     1681 ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat attcaagtgc
     1741 aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa attagggttt
     1801 ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac tcgattttgt
     1861 tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt ctggaaatca
     1921 tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt atccttgcgc
     1981 actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc attatatttt
     2041 tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg ctctaaataa
     2101 atattttcat ccgatttcgt gaagtcaatg tgcttatgcc aatcattggt tttctggcga
     2161 tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt tttaaatgaa
     2221 atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag agtgtaaacc
     2281 tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc tgtcacaatt
     2341 ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg agccgaaagc
     2401 gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata atcgcttatc
     2461 gtaaatccac gtcactaaac attgacgtaa atttactctc actcatctga cttttattat
     2521 tcggtatcac ttcgaataag ccgtcagatt cgtttagata atctcgttcg cttggcgctg
     2581 atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga tcgtcagaaa
     2641 tcgacgatag ccaccgctca aaatcagcaa aagattcaaa cccaaaatgc ttgcgaagcg
     2701 ccttgcccat ttcagcattt tccttctcaa tcaatcgctt tttctgttgg cgaagttttg
     2761 cctgctcttt tttcaatttt tcttcttgtg ctttaatatc agctaaagtt actcccatga
     2821 attacaactc cttaccctta gattcattcc tataaataac accaatttga ttagtcattt
     2881 agcgccctcc acaatttctc gtgcatttcc gccatatcat caagaatagc acgatcccat
     2941 tcagggctat ggtgttcacc gaacacttga aaataagcat tttccacagc tgtctcagca
     3001 ttttccccat ccaaccgacg ataaacagtc accatcatag ccttaaaaag ctgacgattt
     3061 ccgccacaat tacaattatc tttcaccgct tcatatagcc tttgcttatc cagtttcatc
     3121 accctcctta tcatagcgtt ccgcaaagaa tttcttctca tcatccgtca actcacgaac
     3181 ggccaccgag taattggcat ccttttgcat ccagctagca gtcaagaacc agaaatagtc
     3241 gttttcatct ttctggcggt aatgatccag ctcaccggca tcgtattttt tcctgtactc
     3301 cttgaacacg ccattgaaag tcatcagctg acgacctttc atagcaaaat agaacgtgtc
     3361 aaaaacgtct tgggaataag tcatttcaaa atctttggca gaatatttag caatttccga
     3421 taccgccttt tcttgccggt acccattgac cttgcggaca tccaacgaac aaatttcgtc
     3481 ggtaccgtcc tcatttatgc catccaaacc ggtgacatcc cgccacatat ccagccattc
     3541 agcttgggca atataatagc gtttatcagt aaaatatgat ttattaacag ccataatcaa
     3601 gtgaaaatgc gggttataat catttcggct tttgttatac gttatctcca attttcggac
     3661 atagcccttg attgaagtca tcaccctttt tcgacgaaat agcttcgaca gtgctttgtt
     3721 gaaacgatca atttcttcgg ataatttgtc cgccggcacg ttaggcgtgg tgagtgtcat
     3781 aaagatatat tcatagccct tttcagcttg aatagcttcc atcatgatag acaacatata
     3841 gctgtcttta cgggcttttc ggtatg
//
