LOCUS       NZ_CP072575             4372 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB3895 plasmid pVB3895_p8, complete
            sequence.
ACCESSION   NZ_CP072575
VERSION     NZ_CP072575.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533866
            Assembly: GCF_017815675.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB3895
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB3895
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44.3x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815675.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:33:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 35 of 104
            Pseudo Genes (incomplete)         :: 70 of 104
            Pseudo Genes (internal stop)      :: 25 of 104
            Pseudo Genes (multiple problems)  :: 20 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072575.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3895"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB3895_p8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            join(4328..4372,1..189)
                     /locus_tag="J7291_RS15100"
                     /old_locus_tag="J7291_15110"
     CDS             join(4328..4372,1..189)
                     /locus_tag="J7291_RS15100"
                     /old_locus_tag="J7291_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            182..601
                     /locus_tag="J7291_RS15105"
                     /old_locus_tag="J7291_15115"
     CDS             182..601
                     /locus_tag="J7291_RS15105"
                     /old_locus_tag="J7291_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            610..918
                     /locus_tag="J7291_RS15110"
                     /old_locus_tag="J7291_15120"
     CDS             610..918
                     /locus_tag="J7291_RS15110"
                     /old_locus_tag="J7291_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333831.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            922..1284
                     /locus_tag="J7291_RS15115"
                     /old_locus_tag="J7291_15125"
     CDS             922..1284
                     /locus_tag="J7291_RS15115"
                     /old_locus_tag="J7291_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045135959.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            1506..2912
                     /locus_tag="J7291_RS15120"
                     /old_locus_tag="J7291_15130"
     CDS             1506..2912
                     /locus_tag="J7291_RS15120"
                     /old_locus_tag="J7291_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_189660795.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYIYRQREENEREK
                     ALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="plasmid recombination protein"
     gene            3357..4325
                     /locus_tag="J7291_RS15125"
                     /old_locus_tag="J7291_15135"
     CDS             3357..4325
                     /locus_tag="J7291_RS15125"
                     /old_locus_tag="J7291_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002347008.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          3449..4312
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.264"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          1507..2909
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.521"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatttaacag gtgataacgt tgtcgttgtt tttttggata gcaaaaaaat gtccggtaag
       61 ttacttgatg taacgcagta tgagttgatt ttgaaaaata ataaaattga ttccccaatc
      121 attatcccta agcatgctgt taaatatgtt tatggggtag ctgaaagtaa cggagaagat
      181 aatgactgat ttgccagaaa aaatgaccat aaaaaacctt gctgatgagt tgggtatatc
      241 taaaaataca gtgcgcaatc aactggtcaa tgcaggttat gaccttgctc ggtataaaga
      301 taatggggtt atcatgttag acaaggaatt gattggtgtt gtgcggtcaa tttatcggtc
      361 aaaaggtggt caattgactg gtgatttacc ggtcaatgat gcaggcaatg atttcccgtt
      421 agaaaaagaa aatgaatttt tgcgtgaaca attagcggaa aaagataggc atatttctga
      481 attaacggaa atgattcgtg accaacaaaa attgactttg cttcagcaac aaaaaaacga
      541 tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat tctggaagtg
      601 aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat tgaattgatg
      661 tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag tgaaatgctt
      721 gaacgaatta tggatgttct caactcggtt atcaatgctg atctagcaaa atcttcagtt
      781 ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc agaaaaagaa
      841 ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt tattaattcc
      901 aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc gcaagaagaa
      961 aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat tgagaaagaa
     1021 aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt tgctgatttt
     1081 gagcggtggt tatcgtcaat ttctgacgat cagcaacacg aaaaaacagt ggataatgag
     1141 tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga atctgacgac
     1201 ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt tacgtcaata
     1261 tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata aagcataaaa
     1321 ggagttgata gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt gacagcgaaa
     1381 accctcggag agcgtttgga attgtgacat ccatgtcaca attaggaaaa tgttactctt
     1441 tgacaaagag taacagaaag gtttacaatc tttttattag cgacagcgcg aaggagggtt
     1501 ttaacttggg gtttagtatt tcatttaaaa aaggaacgaa gaatacaagt atttctcata
     1561 ataatcgtga gttaactgat cgccagaaaa ccaatgattg gcataagcac attgacttca
     1621 cgaaatcgga tgaaaatatt tatttagagc agacgaatct agcagataaa tatgaggaat
     1681 tgtttggcga agtggtagaa aaatataatg cgaaacaaaa aagagccgat agaaaaatcg
     1741 aggactattt agcgaaagtg cgcaaggata aaaagttgga acctcaacga gaatttattg
     1801 tgcaaatagg cattttagat gatttccaga caaatcgagc agatggttct tctactggca
     1861 tatcgaaaca acaagctgaa caaaatcgag tgattgccaa taaaattttg gttcagtatt
     1921 acaaggagtt tcaagagaga aaccctaatt tggctgttta taatgctgtt attcataatg
     1981 acgaaataag tccgcacctg cacttgaata ttgtgccggt tgctgaaggt tataaacggg
     2041 gtgttcaaaa gcagccgagc tttaataaag cactggttca acaagggata cacgctgata
     2101 aagataatgg tcgagtgctt ttcaataatt ttagaaatca agaagttgac tctatagaac
     2161 gattaatgaa tgattacggt tggaaaaggg atattgtcgg taccaacaaa attaaagata
     2221 ttcacgaata caaagaaatc atgagtgaaa tttctgaatt gcgccaaacg gctgtaaaag
     2281 agcgtgagag cgtttctgcg gaaatgaggt atatttatag gcaaagagaa gaaaacgagc
     2341 gtgagaaggc tctggtggct tctgagagag cggagattga agaagagaag aaaaatatgc
     2401 tgaatgtaaa aaaagagatg gcggtcattt tccaaaaaga tatggttcct ttagaaagtt
     2461 tcaagaggct tcctgatggt tcgtatcatt tatctgggaa tgattttgtt gatttgtatc
     2521 aacgagctac gagagcaaca aatgccaaga gaaatgctga taacatgagt gagcaatatt
     2581 tacagatgct tgagcaaaat acagaattaa atgagtatgt agctgcattg gaagaagagc
     2641 gtcatttgtc tggtagaaaa caattagcag atttacaagt ggaaaataaa aagctgaaag
     2701 aagaaaatcg ctctttacgc cagcgtttag agcgattaaa aaacgcattt caaaaatcga
     2761 tcactcgttt gtctatccgt tttggtcttg cagaaaagga tatggggctt tctgaggaat
     2821 tagaagtatt agagcaggag aaaaccttat tttatcgtca aaacgtctca cagcataaat
     2881 tagagcgttc tgagaatgag attgagtggt agccacgatg tgcagtcaac ggtaccggag
     2941 gcggtttgtg ccgaggataa ggagttgacg gctaaaccac tagcccctga agctgacatt
     3001 tgctaaaaaa tggcaagtcc attttttggc caacttagcg ttagcgaagt ttgggggcgc
     3061 ccttttcaaa gggtgcggtt ttattttttt ggtatttgca tagctaaaga gaagtgctat
     3121 tattgagtta gcaaacaaat gaactggaaa aagagcaaaa aaataagtcg agcaacgcta
     3181 ccaacgttct cgacctttac ggaaactaac cacactagtt ccgtaactca aattcattat
     3241 acgaatattt ttaggtttct tcaagactag agattgatgg ttatgaattt ttagagttac
     3301 gtgaactctg gaaattcttt ccaaaaatct cttttttctt tgaggaggaa ctgaatatga
     3361 gtaaaaaaag aacaatcgtg aatgagtggg aaaaattaac agccctgtct aacaggcggt
     3421 taaatgttca aactttggta caaactataa aattaaattg gtcgattgac cgaattacca
     3481 ttgttggaaa gttgaatcaa aatatttttt atcatactca acatgatgta ttaatattag
     3541 attttgaaca gcttatgcgg gtaaatgaag gcaatggcta tttaaaagcg gttggaaata
     3601 atggttggca gctattagac caacatgacg aaaatattgc ctatattgaa atttctaaat
     3661 ggcaagaagg aaaagggcgg attgatttta atcctaataa aatcagtcga tttttagcgg
     3721 attccatgaa aaattttatt catgatttat ttttagagcc ccatttttca cgagcggatg
     3781 ttgcctgcga tcttattgat gtgcctgatg agtttatcac gcaatatcga attattgatc
     3841 cagtttcatt taaattaatt tatggacgct caggaaaact ggaaacggtg tattggggta
     3901 gtcgggcaag cgagcggcaa attcgtttgt acaataaaaa aatagagcaa gaaatgaaaa
     3961 gaaaggtggt gccttcagaa attaatactt ggtggcggtt agaaatgcag ttaagacgtg
     4021 gtaaagctac ggaatggtat gctatggtga aagaaagttt ggatagcttt gtgagtccac
     4081 attttttacc aactgatata aaacctacgg ataaagttat gattgatgga ttgttagcta
     4141 atcattctaa ttgggcgatt ttgtcaagaa actcgaaata taaatatcga aatttattaa
     4201 agcgagaaag tcaaaatgat gagttgacga atcatttacg agaaacattt gaagaatcag
     4261 cagatgattt aaaaaaagaa ttggatactt ggttacaggg acttgatgtg acagaggagg
     4321 aatagtaatg gcagaaaaac aaaagcattt tggtaagaaa aattggttgg tg
//
