LOCUS       NZ_CP072572             7095 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB3895 plasmid pVB3895_p5, complete
            sequence.
ACCESSION   NZ_CP072572
VERSION     NZ_CP072572.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533866
            Assembly: GCF_017815675.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB3895
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB3895
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44.3x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815675.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:33:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 35 of 104
            Pseudo Genes (incomplete)         :: 70 of 104
            Pseudo Genes (internal stop)      :: 25 of 104
            Pseudo Genes (multiple problems)  :: 20 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072572.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3895"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB3895_p5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            1..933
                     /locus_tag="J7291_RS14965"
                     /old_locus_tag="J7291_14975"
     CDS             1..933
                     /locus_tag="J7291_RS14965"
                     /old_locus_tag="J7291_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="J7291_RS14970"
                     /old_locus_tag="J7291_14980"
     CDS             950..1420
                     /locus_tag="J7291_RS14970"
                     /old_locus_tag="J7291_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="J7291_RS14975"
                     /old_locus_tag="J7291_14985"
     CDS             1504..1956
                     /locus_tag="J7291_RS14975"
                     /old_locus_tag="J7291_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_060806732.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="J7291_RS14980"
                     /old_locus_tag="J7291_14990"
     CDS             2083..3264
                     /locus_tag="J7291_RS14980"
                     /old_locus_tag="J7291_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3354..>3530
                     /locus_tag="J7291_RS14985"
                     /old_locus_tag="J7291_14995"
                     /pseudo=""
     CDS             3354..>3530
                     /locus_tag="J7291_RS14985"
                     /old_locus_tag="J7291_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /name="bacteriocin"
     gene            complement(3680..4828)
                     /gene="tnpB"
                     /locus_tag="J7291_RS14990"
                     /old_locus_tag="J7291_15000"
     CDS             complement(3680..4828)
                     /gene="tnpB"
                     /locus_tag="J7291_RS14990"
                     /old_locus_tag="J7291_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4845..5249)
                     /gene="tnpA"
                     /locus_tag="J7291_RS14995"
                     /old_locus_tag="J7291_15005"
     CDS             complement(4845..5249)
                     /gene="tnpA"
                     /locus_tag="J7291_RS14995"
                     /old_locus_tag="J7291_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            5555..6244
                     /locus_tag="J7291_RS15000"
                     /old_locus_tag="J7291_15010"
     CDS             5555..6244
                     /locus_tag="J7291_RS15000"
                     /old_locus_tag="J7291_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatacct taaagttaat cattaaaaaa ggagtgtcat aatttgaaaa
     3361 aaacaaaatt attggtagct agtttgtgtt tgtttagcag tttattagct ttcacccctt
     3421 ctgtttcatt ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct
     3481 acaaaaaata tccaaaaggt gctaaagtgc caaataaagg tgaactactc accacttagc
     3541 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
     3601 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
     3661 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
     3721 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
     3781 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
     3841 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
     3901 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
     3961 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
     4021 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
     4081 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
     4141 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
     4201 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
     4261 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
     4321 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
     4381 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
     4441 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
     4501 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     4561 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     4621 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     4681 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     4741 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     4801 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     4861 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     4921 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     4981 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     5041 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     5101 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     5161 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     5221 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     5281 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     5341 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     5401 cctaattctg ttaaaaaact ccatcaagag ctattagacc agcgaaataa ggaacagaaa
     5461 aaggtgctta ttcaacgatc attggatttt cttgccccct tatgttgctt agtcctgaca
     5521 ctgttcgcat tttggttagt atggggaggc taaaatggca ttttatttac cctatctact
     5581 catttttgtt agtatttcag gaagtatttg gctcatctat aaaatttttc aaacaagata
     5641 ttcattaaaa ggctctaaaa ttcgatttaa gcgatttttc tttcttggct gtatattttc
     5701 cttgataatc gtttcaagcg gcttattcgg cgttttagag ggaaataagc gcgtttctag
     5761 aagtatttta ttaggaaatg tcactcaaaa gtatgaatca gcgcgtgata aaaagaaaaa
     5821 agagcaagta ttagcacaaa aaatagaaaa atttaccgct tgttatgagg acatgaatga
     5881 tatttttgtg aagcaagaaa agcgtttgac ggataaaaat atggaaacac tcactcgact
     5941 ttatcggaat ttgccagaag aacagcaaaa agagtaccaa gaaaaatatg aacaagtaaa
     6001 aaaagacgtg caatatgtga aagatacgaa agtggaagaa gcttgtagtg atttatttgg
     6061 agatacaatt cctttttcaa ctagcgaaca agaacgaaaa gaacgacaac aatcagtcac
     6121 atatgaacgc tatgaaatct tgcttcaaca agcaattaac atacaaaatc tgactaaaaa
     6181 agaaacagct ttaaattatt taaaaagcgt caaggaatgg ttagatcagc aacagcaaaa
     6241 ttgaatttga ttgaaattca tgttaaacta aacatgcaaa tgttcaaaca ttagatacac
     6301 gaaagcccca gagaggtcgg attctctggg gcttttttta ggttgatgtc gcctttactg
     6361 attttacttc ttgtttcggt tattcaacca ataactgaat acagagatac aaactcctac
     6421 aagaaggggg caaataaaca gttcaaacat cagatacacc tccttccagt cgcaagactg
     6481 tcggataggc gacaagttat tataccctac aaaattaaat ctgtgtggca attttaagta
     6541 tctcttattt ccgggttgtt agggcttgcc ctgaccgcct gtagggcgct ttaaatgcca
     6601 gataaaggtt tttatactaa gactgtctta aaacggctta gacaagctct tagagcctca
     6661 accctccttt ttgaacaatg gttttagcaa aggagctgga ggcgactgcg caggtataat
     6721 gtgagagcgt agcgatcaca cctttttggc gaccgtaggg agcgaggttt tgaattagca
     6781 gtttagaaaa gttcgcgaac ctgtttaaag aaaaaataat tgttttttta ggtgggaaat
     6841 tttgtctccc tcttttataa tcttttttta aacctctttt taaacctctt taccgtcggc
     6901 tgtgtcctta ctcccccaag ggatacaaaa ttttataggc atgaaattat ctgtttatag
     6961 gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     7021 tttaggtatt gtttttagac ataatatgtc ctaaaatgat gtcaataaaa tcatttagac
     7081 tggagtaaca actaa
//
