LOCUS       NZ_CP072571             7974 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterococcus faecium strain VB3895 plasmid pVB3895_p4, complete
            sequence.
ACCESSION   NZ_CP072571
VERSION     NZ_CP072571.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18533866
            Assembly: GCF_017815675.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Complete genome of Vancomycin Resistant Enterococcus faecium_VB3895
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Balaji,V., Yamuna,B. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. v0.4.8
            Assembly Name                     :: VB3895
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44.3x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017815675.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:33:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 35 of 104
            Pseudo Genes (incomplete)         :: 70 of 104
            Pseudo Genes (internal stop)      :: 25 of 104
            Pseudo Genes (multiple problems)  :: 20 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072571.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3895"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVB3895_p4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian medical college"
     gene            complement(join(7778..7974,1..238))
                     /gene="bacA"
                     /locus_tag="J7291_RS14915"
                     /old_locus_tag="J7291_14920"
     CDS             complement(join(7778..7974,1..238))
                     /gene="bacA"
                     /locus_tag="J7291_RS14915"
                     /old_locus_tag="J7291_14920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(468..1616)
                     /gene="tnpB"
                     /locus_tag="J7291_RS14920"
                     /old_locus_tag="J7291_14925"
     CDS             complement(468..1616)
                     /gene="tnpB"
                     /locus_tag="J7291_RS14920"
                     /old_locus_tag="J7291_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(1633..2037)
                     /gene="tnpA"
                     /locus_tag="J7291_RS14925"
                     /old_locus_tag="J7291_14930"
     CDS             complement(1633..2037)
                     /gene="tnpA"
                     /locus_tag="J7291_RS14925"
                     /old_locus_tag="J7291_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            complement(2534..2704)
                     /locus_tag="J7291_RS14930"
                     /old_locus_tag="J7291_14935"
     CDS             complement(2534..2704)
                     /locus_tag="J7291_RS14930"
                     /old_locus_tag="J7291_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846321.1"
                     /translation="MTRKQLLFGSILLGILGFSPMLLSVINKGNKRGELKLNPNYYNGP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2720..3253)
                     /locus_tag="J7291_RS14935"
                     /old_locus_tag="J7291_14940"
     CDS             complement(2720..3253)
                     /locus_tag="J7291_RS14935"
                     /old_locus_tag="J7291_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_125291180.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEQLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(3274..4224)
                     /locus_tag="J7291_RS14940"
                     /old_locus_tag="J7291_14945"
     CDS             complement(3274..4224)
                     /locus_tag="J7291_RS14940"
                     /old_locus_tag="J7291_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            4900..4986
                     /locus_tag="J7291_RS14945"
                     /old_locus_tag="J7291_14950"
     CDS             4900..4986
                     /locus_tag="J7291_RS14945"
                     /old_locus_tag="J7291_14950"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5107..5796)
                     /locus_tag="J7291_RS14950"
                     /old_locus_tag="J7291_14955"
     CDS             complement(5107..5796)
                     /locus_tag="J7291_RS14950"
                     /old_locus_tag="J7291_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6041..6955)
                     /locus_tag="J7291_RS14955"
                     /old_locus_tag="J7291_14965"
     CDS             complement(6041..6955)
                     /locus_tag="J7291_RS14955"
                     /old_locus_tag="J7291_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6937..7317)
                     /locus_tag="J7291_RS14960"
                     /old_locus_tag="J7291_14970"
     CDS             complement(6937..7317)
                     /locus_tag="J7291_RS14960"
                     /old_locus_tag="J7291_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3275..4223)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6045..6955)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagcaggagt ctttagctta ttataatttt ttttcaaatc tctacacatt ttattagtaa
       61 cataatagtt cattttatag caagtaaatc cacgatgatt atgattatat cgtgaacttg
      121 cagcttccac ttttatacct ctagcactaa aaggaaacag atttaacgga gtacttgctc
      181 caacaatcat accgaacaaa aatagacaag taataatttt aataatttta tttttcattg
      241 ttacttcctc cttagatata aaatcctagg aaagcgcttc ctgtatatat ttagaataat
      301 cagaaaagtg aactactcac cacttagcta aacctgacgg ttcttaacgc ttgaagtggg
      361 agcttcttgg gaatagagca tacttgatag cgtatttctt tactaacagc ggtgtctctt
      421 atttaccaag ctatccccgt agttcctacg gttcttgatg attggatcta agccaatccc
      481 atagaatgta gtcttagacc ttcggtcaga atattgatac tagcattgat atcacgatca
      541 tgatgagtat gacaaacagg acaagtccat tctcgaattt caagagattt cttgccatct
      601 ttatgtccgc actctgaaca aatctgacta gacggaaacc atttatctac tttgatgatt
      661 tttcgtccat accagtcagc cttgtattgt aatttagtca caaactttga ccaagaaaca
      721 tcagaaatac ttttagctaa tttatgattt cgcaacatac cttttgtgtt taagtcttca
      781 atacagataa tatcgtgatt tttgatcatt tctgtactca acttattcag aaaatcagta
      841 cgttgattca ttactttttc atgcaatcta gctactttgc gtttttgttt ttgataattt
      901 ttagcttcaa atagattggt acctttattt ttagccaata aagcacgcct agacaatttg
      961 cgttgttcac gctttagttt cttttccatt ttggacgtga atttattatt atcaattttt
     1021 tgtccatcag aaagaatcgc aaagtccata atacctaagt caataccaat tgtggaatta
     1081 gttttaggta attcgataat ttcttcttta cacaacaaag aaatatagta tttaccgcta
     1141 gaatgacgtg atattgtagc agatttgata atcccttttg gttgtctatg aagcttaatt
     1201 cttactaatg acttcaattt aggaactttg atgaatttac tatcaatcaa agcaactgta
     1261 ccattttgat tattcgttgt ataactctga acaggatttt tcttactttt gaaacgtgga
     1321 aatccaacgg atttatcccg aaaaaaattc ttgtatgctt tatctaaatt aagttgggca
     1381 ttggctaaag caaggctatc aatttctttc aaaaacggaa attctttctt gtatttagct
     1441 ggtgtcggaa aagtcatttt tttagaagaa tcatttttaa cttcttcata cgcctttttt
     1501 cggtcatcaa gcattagatt gtagacctta cggacacaac caaaagattt ggcaaagaag
     1561 atttcttgtt cctctgttgg atagattctg aatttgtatg cttttagttg ttccataaag
     1621 ttcacctact ttctatttat gattgtttcc ttgattttgg atatacttct taatcacatc
     1681 aattggtgcg ccaccagttg tcagaagaca aaaactttta gaccagaaca tttctttcca
     1741 aagaaattgt ttgactcttg gaaagtcacg ttttatcaat cttgagctgg cacttttata
     1801 ggcattgatg aattttgtca attctgtttt aggttgagct ttgaacataa tatgaacgtg
     1861 gtctttatca tgattccact ccactaaagt aatgtgatac gattctgaaa ttctttcaaa
     1921 agtagttttg gcataatcag atatttcatc atcaatcact tgtctacgat atttcgttac
     1981 taaaacaagg tggtaataaa gaagaaatac tgaatgatta tttgtatcta attccatgtt
     2041 atatcaactc atttcttgta tagactgatt gtaacatgga acaaaataaa aaggcaatac
     2101 gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg gcattcatca
     2161 cccacctata gaggatgggc gacttctgcc taattctgtt aaatattgtc aagagggttt
     2221 attttataaa acgtgttatt ttaaaggtta gtagacataa tgtacactag accaagttgc
     2281 ccctctacca atggatagag acgctcactt tgtgattttt aattggtcta ggtcaaaagc
     2341 tctttttaag gcttgatttt ctgccgttgc ttctttccgt ttctttgtga atttttacca
     2401 gtcatttttt actggggtgt tatttttcat aaaatatgat aaatttttgg tagcgcttca
     2461 taagcgcgtt gctttttcaa gaataaacac tcattatttt ttaactggct gctcgaaaga
     2521 gcagtctttt ttattattta tctgtttctt ttacaaacaa ttcttcatta ggaccgttgt
     2581 aataatttgg attcaatttt aattcaccac gtttattccc tttattgatc actgacaata
     2641 acatcggact gaaaccgaga attcctagta aaatcgaacc gaatagaagt tgttttcttg
     2701 tcatgaatgt tccctccagt tatttccata gttgccacca ttttttttca gtatcaattt
     2761 gtttgtctat tgtttctgtt gcttcagaaa gagtcgttga gtcattctct ggagcttctt
     2821 ctgtataagt caactgtagt tgttcttgca gtttttcgat ttgtttgttg gcttgtaggg
     2881 tgagttgttg ttgctggtcg agtagttttt gtatatgaga attattttgc tctctgaggg
     2941 caagttgttc tcgtaaaaat tcattttctt cacgcaactg atctagcaac ttgtttgtaa
     3001 ccacgttttt gcttggtata ttaacatttt tatcgttgtc ttgttcgtac tcgtgcaacc
     3061 tatctagcaa catttggttg cctaagttgc gagcaagttg caatactcca gaacgcaata
     3121 caaccttata accacgctca tttttagcta tcatggaaga atctaatttt tggatatgtt
     3181 tcgatattgc ttgcctactc atcttaaaaa gttctgacaa ttcctttatt gtaaaggttt
     3241 cttgtggttg catactggtt gcctcctagg ttgctactgg aataaattat ctaaatcaga
     3301 taataaactt tttttctctt ttttcgaaag tgcggactct gtttgttttt caagagcttc
     3361 tttctctttt tctagttgtt tgatttgttg gaggagttca agttctcgtt tcttctgttt
     3421 gattttttcg ttaattgttg tagcaggttc ttcgtattct ttttgtttat cagctaaagc
     3481 caatgcttta ttttgttctt ctttggttgg tgcatgttta ttaaaatcat ctgctttttt
     3541 gatttctggt ttaaacgtaa attgatagcc aacgacaggt tttcctcgcc cttttccgta
     3601 tttctttttg attgataatc ctttgaagat tgcggttaat tcctgtcgta taggctttat
     3661 aacacgatta tcaacgtttg cttggttgtt ccagtaactt tttggaatat ctaatagttc
     3721 atgaaaatcc tctttagaaa aataagcaaa acctttagtt cgatattgtt ttagtaatcg
     3781 aaacatagtc tttgcatagc tactttgtaa ttcgttaaat tgttggagtg aatagcgaac
     3841 ccactcatct aaattgttta gtaaaggtaa agctttttcg taaacttgta tgtctacata
     3901 tggatcttta gcagttccac gtattttaaa ctcactaaac ataacgaatc tatctatatc
     3961 gagcccatta gcacttcttt taccaaaacg taaatccatt aatttatcat aagtcatttc
     4021 taaatcatta atgaaacgat tattagcagt tgctttataa ttacttaaat cttttagttg
     4081 gtcaaaagta agacggactg ttttatcgcc tttttcgcgc attctagaca caatagagaa
     4141 aaatagattc atttcaactg gtgtaaattt ccgcaacgga atggtattta attcatgatg
     4201 gtatttcacg atttcatttg ccaaagatat tcctccttac tccttcaatg gatacagacg
     4261 ttataaacaa cgttttgtct gtgataatac atattataac atacgtgagt gcaaaaaaag
     4321 agtttaaaag cagacaaaat gtagtttaaa agcagacaaa atgtagttta aaagcagaca
     4381 aaatgtagtt taaaagcaga caaaatgtag tttataaaac gctgtatcca ttgaaggagt
     4441 aaggacacag ccgacggtaa agaggtttaa aaaaaggttt aaaaagagat tataaaagag
     4501 ggtgacaaaa tttcccgcct aaaaaccact tcttttttct accaacaggt tcgcgcattt
     4561 ttcaaaactg ccaattcaaa accttgctcc ctacggtcgc taaaaaagtg tgatcgctat
     4621 gctctcacat tgtactgcgc agtcgcctaa aggctccttt gctaaaaaca agtcagtaac
     4681 ggaggtgggg gaagcctctg atagctcgtc taagtcgttt taagacagtc ttgggtaaaa
     4741 aaccttatct tgttcttaaa gcgccttaca ggcggtcagg gcaagcccta acaacccgaa
     4801 aataagggat acttaaaaac accacacaga ttttattttg taaggtataa taacttgtcg
     4861 cctatccgac agtcttgcga ctggaaggag gtgtatctaa tgtttgaacg gttcatttgc
     4921 ccccttcttg tagaagtttg tgtctcttta ttcagttatt ggttgaataa ccgaaacaag
     4981 aagtaaaatc aataaaggcg acatcaacct gaaaaaaagc cccagagaat ccgacctctc
     5041 tggggctttc gtgtatctaa tgtttgatca tttgcaggtt cagtttaaca tgattgctaa
     5101 tcaaattcaa ttttgttgtt gctgatccaa ccattccttg acactttcta gataatttaa
     5161 tgccgtttct tttttcgttg gattttgtat gtttgttgct tgctgaagca gtgtttcata
     5221 acgttcgtat gtgaccgatt gttgccgttc ttttcgttct tgttcactgg ttgaaaaagg
     5281 gattgtatcg ctaaataagt tacaacaagc ttcttctgtc tgcgtatctt taaaatattg
     5341 cacgtctttt tttacttgct catatttttc ttgtacctct ttttgctgtt tttctggtaa
     5401 gttttgataa aaacgagtga gttttttcat gtttttatct gttaaacgtt tctcttgatc
     5461 cacaaaaata ttgttcatgt cctcataaca agcggtaaat tcctccattt tttgtgctaa
     5521 tgcttgctct tttttctttt tatcacatgc tgcttcatac ttttgagtga tatcccctaa
     5581 taaaatgctt cttgaaacac gtttattacc ctctaaaacg ccgaataaac cacttaaaac
     5641 gattgataag gcaaatataa ccccgaccaa taaaaatcgc ttaaatcgaa ttttagagcc
     5701 ttttaatgaa tatcttgttt gataagcctt atagctaagc caaataattc ctaaaatact
     5761 ggcagaaatg agtagatagg gtaaatagaa tgccatgtct gtttattctc cccataccaa
     5821 ccaaaaggct aataatgtta gtactaagca acataagggg gcaagaatat ccatcgatcg
     5881 ttgaattaaa gccttttttt gttcttggtt tcgctggtct aatagcgctt ggtggagttt
     5941 tttgacttgt gaatccatcg tagtttgtat cctttcttgg gtataaggcg caagaaaggc
     6001 acgttgtctc tttatttcgt tttcgatgtc ttttaacgtt ttagagcgat aattcttgtt
     6061 ggtggtctgt tcctcgttca acgtgttgtt catggtttct tcgagtcggt ctaatttccg
     6121 attgttcagg cgtaaaagtt cgttgttctc ttcgtacttc ttctgcagtt tcgcgaatgc
     6181 tagctcgagc tcttcgtagc tcgttggttc gttgttgatt gtaggttcgt tctcgtctgt
     6241 ccagctcatg taaaatcgtc tccttttcgt aatcctttcc tagtttgttg gatcgtactt
     6301 ttttcttttt gtggaccaat tcgtaagtaa aattttttcc gcgattcaag caatgaatgc
     6361 cgaatgtttt aagtgaatca caaaattggg tatacgttcg agtttttttg tttttcattg
     6421 tttgatcaat cgcaaaccga atttcgtctt tccaaaccgg ttcatttttt tcttttaatt
     6481 taatttcaga catggttcgt ttttcggttt tctttcctcg ttccgtgatt gttaagtcat
     6541 gttcaagaca aatctcgtca tttaggtcat aacatttgtt taaaaatgct tgcccatggg
     6601 cttgaaattt tttcccagtt tcaagattaa cggagttaat gacaatgtga ttatgaatat
     6661 ggtctttgtc tgtatgtgtg taaatcgcga tttggtggtt aggggctatt tttttcgcat
     6721 actctgcccc tagttgattg gctttttcag cggtaatttc tcctggtcga aaggactgaa
     6781 tcaataagtg tgcttgtaca cgatcgtctt ttccataaag cgtgcgtatt tgtttcattt
     6841 cacttttggc ataatcaatg tcacagttta ttcctgtttt gacggttgct cgtggttctg
     6901 cgtaattgat tgctcggcta catgaagtgg ttcgacttac tttaactgtt gccatatctt
     6961 ttgtagctcc ttttgcatga tttttaggtt ttcggacaaa cgttgagcat catcaggagc
     7021 aacgtcgtgt tggtgtgtat tgcaccactt ggcaatttga ttgatattcc ctccagcttt
     7081 ggctaaatcg cgcgcgattg cctgggcaat gtctggcgcc actttcggtg tcaccagacg
     7141 cgcgttttgt gccttggctt tcacgaaagc cggcacactc atttgcaaag cttcagctga
     7201 ctgcttcagc tttgcaaatt cggattcgct cactcgaaaa ctgatttgtt ttggctcgga
     7261 tcgataggta tattttttag aatgatcgct agccaaattt tgatgttgtt cgctcataag
     7321 tcccaccctt tcagtacgtc ctacggacgt atcgttcgca ataccattgt acctaaaaag
     7381 gtacaatgag taaagctcac taaaagggtt tggcaagctc tatatttgct agccaaatac
     7441 ctccgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc
     7501 ctcaaaaccc acgctccaca atacaaaaag tggctagcat tttctagcca ctttttgtaa
     7561 aatttagtat gatttatttt tagctattta gagtttttat ttccgtaaat acttctaaaa
     7621 attgctaaga taaatgctat aggaattaga taaagaatac ttttttgtaa gcttatattg
     7681 aaaattagcc taaatatgaa gaacatagtg attaaaacta ttattgaaat aattacagct
     7741 aaaatctttt tattcatgag atagtttcac tttctgatta ttttattaca taacgagcgt
     7801 aatcattgta ttgatatgat gttgtattgc taaagtgagc attatatgtt aattgtacac
     7861 cttttccttg atttgcagct cttacaaaga cattcattgc atcaacagca ctactaaaag
     7921 catttgtaat aacccatgtt ccaactccac caataggaat aaaagatgca attt
//
