LOCUS       NZ_CP072545             2454 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli O128ac:H12 strain AW1.7 plasmid pAW1.7_6, complete
            sequence.
ACCESSION   NZ_CP072545
VERSION     NZ_CP072545.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18530112
            Assembly: GCF_018085225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O128ac:H12
  ORGANISM  Escherichia coli O128ac:H12
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Complete Closed Genome Sequence of Extremely Heat Resistant
            Escherichia coli AW1.7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2021) US Department of Agriculture, US Meat Animal
            Research Center, State Spur 18D, Clay Center, Nebraska 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-APR-2020
            Assembly Method :: Microbial Assembly v. SMRTanalysis v8.0; SKESA v.
            v 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 01:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,034
            CDSs (total)                      :: 4,915
            Genes (coding)                    :: 4,644
            CDSs (with protein)               :: 4,644
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 75 of 271
            Pseudo Genes (incomplete)         :: 170 of 271
            Pseudo Genes (internal stop)      :: 62 of 271
            Pseudo Genes (multiple problems)  :: 36 of 271
            Pseudo Genes (short protein)      :: 3 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072545.1.
            Bacteria and source DNA available from Joseph M. Bosilevac, US Meat
            Animal Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Escherichia coli O128ac:H12"
                     /mol_type="genomic DNA"
                     /strain="AW1.7"
                     /serotype="O128ac:H12"
                     /isolation_source="Cattle slaughter plant"
                     /db_xref="taxon:2822132"
                     /plasmid="pAW1.7_6"
                     /geo_loc_name="Canada: Lacombe"
                     /collection_date="2001"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            complement(join(2270..2454,1..43))
                     /locus_tag="J7886_RS24945"
                     /old_locus_tag="J7886_24940"
     CDS             complement(join(2270..2454,1..43))
                     /locus_tag="J7886_RS24945"
                     /old_locus_tag="J7886_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            897..1010
                     /locus_tag="J7886_RS24950"
                     /old_locus_tag="J7886_24945"
     CDS             897..1010
                     /locus_tag="J7886_RS24950"
                     /old_locus_tag="J7886_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059570819.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(1239..1538)
                     /locus_tag="J7886_RS24955"
                     /old_locus_tag="J7886_24950"
     CDS             complement(1239..1538)
                     /locus_tag="J7886_RS24955"
                     /old_locus_tag="J7886_24950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016247612.1"
                     /translation="MKKILFCSLLLLSASSFAQQFHYQCEGRDLKIMLSPNAAFLDGKS
                     FEYKNGETNGETTKMTFFGPSGEVLKFLVNPVNNLYRMGLTSGATFEEINCTEI"
                     /name="hypothetical protein"
     gene            complement(1562..1801)
                     /locus_tag="J7886_RS24960"
                     /old_locus_tag="J7886_24955"
     CDS             complement(1562..1801)
                     /locus_tag="J7886_RS24960"
                     /old_locus_tag="J7886_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560795.1"
                     /translation="MIKKICITVIVVFLLLVGYGAWIGSEQNQRGVSLFEVAYTYNAMN
                     PISRIGYTFMLKRNHALVERAGEVKKSIDSMSGE"
                     /name="hypothetical protein"
     gene            2034..2222
                     /locus_tag="J7886_RS24965"
                     /old_locus_tag="J7886_24960"
     CDS             2034..2222
                     /locus_tag="J7886_RS24965"
                     /old_locus_tag="J7886_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          516..1006
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.929"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 cggggcaaag gttcataagt tctcctggtg aaaaaccaaa catgaagcac ccatctatta
       61 acgtcagctg tgaacgcttc attagaagaa aataggtaaa cccgggaaaa agatccaaac
      121 gttaacatca caggtaaaca gaaacgtcgg gtcgatcggg aaattctttc ccggacggcg
      181 cggggttggg caagccgcag gcgcgtcagt gcttttagcg ggtgtcgggg cgcagccctg
      241 aaccagtcac gtagcgctag cggagtgtat actggctaaa cttcagagat gccaaggaag
      301 ggacaggata tgtgttgtag caccgccacc acggtggcga tactccaaaa cgcctggtca
      361 gggcacagcc ccgacacccc agagccgact gtaggtcact ggacgaacgc ggaggaatga
      421 tgtcggatga cgtcattttg cggcgtctgc cctatcctgc atcgtattaa aaaatgcagc
      481 tatggcgcgt acggtgccaa agataagccc ggagatattc cgcttcctcg ctcactgact
      541 cgctccgctc ggccgttcgg ctgcggcgag cggtaccggc ttactggcgg ggcggaaatt
      601 tcctggcatc ttccaggaag aagcttaaca gggaagcaag aaggccacgg cgaagccgtt
      661 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
      721 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggtggct ccctcgtgcg
      781 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      841 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      901 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      961 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
     1021 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
     1081 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
     1141 ccgccttgca gggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
     1201 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
     1261 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttgac aggattaacc
     1321 aaaaacttga gaacctcacc agaaggacca aagaaggtca tctttgtcgt ttccccattc
     1381 gtctctccat tcttatattc gaatgattta ccatcaagaa aagctgcatt aggtgaaagc
     1441 attatcttta aatctcgtcc ttcacactga taatggaatt gttgtgcaaa tgaacttgca
     1501 gaaagaagaa gcagcgaaca aaaaagtatc tttttcatat cagtccttta aatttaaaaa
     1561 atcactcacc actcatgcta tcaatagatt tcttaacctc tccagccctc tcgactaaag
     1621 catgatttct cttcagcata aatgtatagc cgatccgact aattggattc attgcgttgt
     1681 atgtatatgc aacctcaaat aaagatactc ctcgctgatt ttgctcagat ccaatccaag
     1741 caccatagcc aaccaacagc aaaaaaacga cgataacagt aatgcagatt ttttttatca
     1801 ttccctatcc agataatatg aagaaaacag aattgaacat aacatccgtt gcccgcacca
     1861 cctcaggcgc cctcagggcg tttctgagga ctccggcctg aaacatacac ctttctgtgc
     1921 ccatatcgag aaaaaacgcc cctgatgatt tctgcgtacc tgcatggcta tgcactcaag
     1981 tgaatgaagt aagatttcag tctgcgatca ccataaaacc gggaaaaagc cgtatgacaa
     2041 aacaggaatc agccgcactg aacatggcaa aatttatccg ggcgcagtcg ctgctgctgc
     2101 tcgaaaaact cgacgtgctc gatcttgatg acgaggcagc cgactgtgag cgaatgcacg
     2161 aacaggcaga agcgctttat cagaagctga gcgcacgctt cggcatacag gacggcgaat
     2221 aaagagacga aaagaacgct cacttagacg aaaagcgtat ctatggcaac tatgtatatc
     2281 caccgtgtac atagtcatgc ctgacgcttt cgagcaggtc gagcatgtag gaacgacgag
     2341 cttgattggt atcggacaca atatggcgat acgcctcaaa cataacagca cccgcatcac
     2401 cgcccgaacg gcgaagcata gcggaaatca tccgcacaaa gagcagacgt tcat
//
