LOCUS       NZ_CP072544             3211 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli O128ac:H12 strain AW1.7 plasmid pAW1.7_5, complete
            sequence.
ACCESSION   NZ_CP072544
VERSION     NZ_CP072544.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18530112
            Assembly: GCF_018085225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O128ac:H12
  ORGANISM  Escherichia coli O128ac:H12
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Complete Closed Genome Sequence of Extremely Heat Resistant
            Escherichia coli AW1.7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2021) US Department of Agriculture, US Meat Animal
            Research Center, State Spur 18D, Clay Center, Nebraska 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-APR-2020
            Assembly Method :: Microbial Assembly v. SMRTanalysis v8.0; SKESA v.
            v 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 01:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,034
            CDSs (total)                      :: 4,915
            Genes (coding)                    :: 4,644
            CDSs (with protein)               :: 4,644
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 75 of 271
            Pseudo Genes (incomplete)         :: 170 of 271
            Pseudo Genes (internal stop)      :: 62 of 271
            Pseudo Genes (multiple problems)  :: 36 of 271
            Pseudo Genes (short protein)      :: 3 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072544.1.
            Bacteria and source DNA available from Joseph M. Bosilevac, US Meat
            Animal Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Escherichia coli O128ac:H12"
                     /mol_type="genomic DNA"
                     /strain="AW1.7"
                     /serotype="O128ac:H12"
                     /isolation_source="Cattle slaughter plant"
                     /db_xref="taxon:2822132"
                     /plasmid="pAW1.7_5"
                     /geo_loc_name="Canada: Lacombe"
                     /collection_date="2001"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            complement(979..1191)
                     /locus_tag="J7886_RS24920"
                     /old_locus_tag="J7886_24915"
     CDS             complement(979..1191)
                     /locus_tag="J7886_RS24920"
                     /old_locus_tag="J7886_24915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(1301..1567)
                     /locus_tag="J7886_RS24925"
                     /old_locus_tag="J7886_24920"
     CDS             complement(1301..1567)
                     /locus_tag="J7886_RS24925"
                     /old_locus_tag="J7886_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1551..1775)
                     /locus_tag="J7886_RS24930"
                     /old_locus_tag="J7886_24925"
     CDS             complement(1551..1775)
                     /locus_tag="J7886_RS24930"
                     /old_locus_tag="J7886_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2321..2506
                     /locus_tag="J7886_RS24935"
                     /old_locus_tag="J7886_24930"
     CDS             2321..2506
                     /locus_tag="J7886_RS24935"
                     /old_locus_tag="J7886_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_054626234.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLEKLDVLDLDDEAADCERMHEQAE
                     ALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2554..2793)
                     /locus_tag="J7886_RS24940"
                     /old_locus_tag="J7886_24935"
     CDS             complement(2554..2793)
                     /locus_tag="J7886_RS24940"
                     /old_locus_tag="J7886_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205582579.1"
                     /translation="MGAFMFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFE
                     AYRHIVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          44..533
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.13"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 aatgcagcta tggcgcgtac ggtgccaaag ataagcccgg agatattccg cttcctcgct
       61 cactgactcg ctccgctcgg tcgttcggct gcggcgagcg gtaccggctt actggcgggg
      121 cggaaatttc ctggcatctt ccaggaagaa gcttaacagg gaagcaagaa ggccacggcg
      181 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
      241 cagtggtggc gaaacccgac aggacttaaa gataccaggc gtttccccct ggtggctccc
      301 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
      361 gtttatctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
      421 tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt
      481 gagtccaacc cggaaagaca cgacaaagcg ccactggcag cagccactgg taacaggttt
      541 agaggaggag agtcttgaaa tttcgggtgc gataaggcta cactgaaagg actattttgt
      601 gactgtgctc ctccaagcca gttaccgcgg ttcaaaagag cgtcaactct tgcgaacctt
      661 cgaaaaagcc accttgccgg gtggtttttt cgtttacaga gctaagagat tacgattctc
      721 cctaaatgat ctcaagaaga tccgtttaaa aaagcgggtt ttatggttgg atgaagttcg
      781 tcttacccag tcacatatta gatttatttg aattactact aggtttcgta agccatgcaa
      841 gaattcctgt aactaacaaa ataggccctg caatgtacgt aacatagact ccaacatacg
      901 acactgtgaa acatatgagt acgtatatag ccagtgttgt ccaaaaaatg actgccgttt
      961 tacagtgaat ccactctttc atttttcaat aaatcccaca ccgtctggag atcttactat
     1021 cgttttacca ggattagctt tcgcccaaac caaagcatct tgaaaagaag agaattcttt
     1081 tttcgaattt ttagcttttt ggcgctcatc tcgaaaatat tcgctgttat tctcatcaat
     1141 tatccgatca aaatcagcac cagctccgta ggtacgctca aaatccccca tttcctaccc
     1201 ccataaattt aataaaaaaa cagatttaaa atatccaaca gacactctta tttaccataa
     1261 catccgttgt cagcatcaaa aaaagcccgc atagcgggct tcagtcgtaa acttcgcggc
     1321 gatggccaat ggtggcggca aggataacca gctcgtcatc ccggatctca cacagaatgc
     1381 gataatctcc cacccgatag cgccagaact cgcccagctc acctttgagc ggcttccctg
     1441 actggcgagg atcggcagca actgcaatgc gtaaatccat aaaatccaca atccgtcgtg
     1501 cgttctgttt gtccatcttg cgtaacgatt tgagcgcccg gtcggaatag ttaatcgtcc
     1561 agaccaagtg acttcctcac gtcttcagac gaatgcacgg cttcatcacc acggcgaacg
     1621 cgtgcggcag tttctgctga aagataataa tcttccaggt cttccagatg cgccagtatt
     1681 gcttcccgcg catagaacgt ctttgttctg ccggtttgct tcgccagcga gtccagacgg
     1741 gactcaatct catcagataa ccggatagca agcatataaa cctcctgaac accatttgat
     1801 atacatgtat agcaatagta tcgcaatcaa cctaaaagaa caaaaaataa tcttgctggc
     1861 ccctcttgat aatccgtaac gctgtcaata tatccagctt gagggctttc ctcttaacta
     1921 ttgaaaggat aacgattatg ggtcttaagc cgggtccgaa acgaattgcc gattcaacag
     1981 gggaacctga taaacgccag cgtgacaata aaaaaacgcc ggggaatact gacaaattga
     2041 aacccagcaa atcctcaaaa aaataagtca gtagttaaaa gccctccggg gctttttttg
     2101 tacccgcaga tcagtctgat tgaacataac atctgttgcg tgcaccgcct gagaggctct
     2161 cagggcgttt ctacgacctc acctgcaaaa gaacacatct taccccctga aaaccgctca
     2221 gaaacgcgct gatgatttct gcgtacctgc atggctatgc actcaagtga atgaagtaag
     2281 atttcagtct gcgatcacca taaaaccggg aaaaagccgt atgacaaaac aggaatcagc
     2341 cgcactgaac atggcaaaat ttatccgggc gcagtcgctg ctgctcgaaa aactcgacgt
     2401 gctcgatctt gatgacgagg cagccgactg tgagcgaatg cacgaacagg cagaagcgct
     2461 ttatcagaag ctgagcgcac gcttcggtat acaggacggc gaataaagag acgaaaagaa
     2521 cgctcactta gacgaaaagc gtatccaggg caactatgta tatccaccgt gtacatagtc
     2581 atgcctgacg ctttcgagca ggtcgagcat gcaggaacga cgagcttgat tggtatcgga
     2641 cacaatatgg cgatacgcct caaacataac agcacccgca tcaccgcccg aacggcgaag
     2701 catagcggaa atcatccgca caaagagcag acgttcatcg gggcaaaggt tcataagttc
     2761 tcctggtgaa aaaccaaaca tgaaagcacc catctattaa cgtcagctgt gaacgcttca
     2821 ttagaagaaa ataggtaaac ccgggaaaaa gatccaaacg ttaacatcac aggtaaacag
     2881 aaacgtcggg tcgatcggga aattctttcc cggacggcgc ggggttgggc aagccgcagg
     2941 cgcgtcagtg cttttagcgg gtgtcggggc gcagccctga accagtcacg tagcgctagc
     3001 ggagtgtata ctggctaaac ttcagagatg ccaaggaagg gacaggatat gtgttgtagc
     3061 accgccacca cggtggcgat actccaaaac gcctggtcag ggcacagccc cgacacccca
     3121 gagccgactg taggtcactg gacgaacgcg gaggaatgat gtcggatgac gtcattttgc
     3181 ggcgtttgcc ctatcctgca tcgtattaaa a
//
