LOCUS       NZ_CP072543             1935 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli O128ac:H12 strain AW1.7 plasmid pAW1.7_4, complete
            sequence.
ACCESSION   NZ_CP072543
VERSION     NZ_CP072543.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18530112
            Assembly: GCF_018085225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O128ac:H12
  ORGANISM  Escherichia coli O128ac:H12
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Complete Closed Genome Sequence of Extremely Heat Resistant
            Escherichia coli AW1.7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2021) US Department of Agriculture, US Meat Animal
            Research Center, State Spur 18D, Clay Center, Nebraska 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-APR-2020
            Assembly Method :: Microbial Assembly v. SMRTanalysis v8.0; SKESA v.
            v 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 01:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,034
            CDSs (total)                      :: 4,915
            Genes (coding)                    :: 4,644
            CDSs (with protein)               :: 4,644
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 75 of 271
            Pseudo Genes (incomplete)         :: 170 of 271
            Pseudo Genes (internal stop)      :: 62 of 271
            Pseudo Genes (multiple problems)  :: 36 of 271
            Pseudo Genes (short protein)      :: 3 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072543.1.
            Bacteria and source DNA available from Joseph M. Bosilevac, US Meat
            Animal Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Escherichia coli O128ac:H12"
                     /mol_type="genomic DNA"
                     /strain="AW1.7"
                     /serotype="O128ac:H12"
                     /isolation_source="Cattle slaughter plant"
                     /db_xref="taxon:2822132"
                     /plasmid="pAW1.7_4"
                     /geo_loc_name="Canada: Lacombe"
                     /collection_date="2001"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            join(1730..1935,1..781)
                     /locus_tag="J7886_RS24905"
                     /old_locus_tag="J7886_24900"
     CDS             join(1730..1935,1..781)
                     /locus_tag="J7886_RS24905"
                     /old_locus_tag="J7886_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            1441..1743
                     /locus_tag="J7886_RS24910"
                     /old_locus_tag="J7886_24905"
     CDS             1441..1743
                     /locus_tag="J7886_RS24910"
                     /old_locus_tag="J7886_24905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          complement(508..696)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 gctacgtgag gcgcatttct gtcgtgtccg gcactgtcct gtctgccagt ggaggcgatc
       61 cctgatgtgg caagcccgtt tttatcagtc cctgccgcgt attgttgcgg attatccaga
      121 tgcacgctgg atgttcctga ccctcacagt gcgaaactgc gcgatcgggg aattgggtga
      181 gacacttaac cgaatgaaca tggccttcca gcgacttaag gatcgcaaag agtttcgacc
      241 tgttcagggc tggatccgca ccacggaagt tacacgtgga agtgacggta gtgcgcaccc
      301 gcactttcac actctgatga tggttcctcc ctcaatgttc acgcgagatt acgttaaaca
      361 tgaccgctgg gtagagcttt ggcgagagtg tttgcgtgtg aattacgatc cgaatgttga
      421 tgttcgagca gtaaaacccc gcaagcctaa ggacggagaa tcattggctt ctgcaactgc
      481 ggaacttgta cggggcgcag tggctgaaac cctgaaatat tccacgaagc cggctgacat
      541 ggttgctgat cctgaatggt tcctcgaatt gactaagcag acacataagc gccgatttgt
      601 ggctaccggt ggcgcactca aagacatact gaaactcgat caggaaactg atgccgatat
      661 ggtaatcggg gatgatattc ccgaaggcga tgatgatggt tcacgcattg cttttgagtg
      721 gaaaaccgaa tccaaaaagt accgtcgctc accgtcaaaa gataaagctg aatcagactg
      781 acagggtgtc ggggcgcagc cctgaccacg acgtttttga gaggacgccg cttgtcggcg
      841 gtactccaaa aacacatctt gtcctattgg ttctactccg atgcaggata gggctaacgc
      901 cgcaaagtga cgtcactcaa ttactgagtt cggtttccga actcagttgg gacaagatgt
      961 gtttttggta caccgccatc acgatgtcgg tgccaaaaac gattcgtcag ggctgcgccc
     1021 cgacaccctg ccaacacctc acgcagtcag attaaaatct gccagcgctc gctgtttccg
     1081 aaatcgtcaa actctaaatc gtggggtccc gctggcttgc cagtgggttc cgatttagtg
     1141 attgacgaca ttgctgattg aaaaattggc tgaggtggtt ggattcacga agtgaggcca
     1201 accacctgga agacggtttt taattgtctg gtaccggata caaccagaac gaatggagtt
     1261 ggttctgtcg tgatgcgaaa aagtatgggt tcgcaaatca cagactaaat agatttaccc
     1321 aattccattc gagtgaggtt gttatgtttt ttgaggttct taatcatgtt gatagtgcgt
     1381 tgagcgttgc tgtttcagct cctggcgttg tggcagtttt tggtggcggg tgggcgttga
     1441 atggctggtt cgatccgcgc gcgatccttg tatttatact taagggataa ctctggcgat
     1501 aaaaaatggc atcaagcctt gcgtaccaag gcttcggggc ttatttgcgt tgtagaaggt
     1561 tttggaagta ttctgttggg agtggtcgcc ttaaaagcga cccccgcaac gacggccatc
     1621 gttcgggggt gtgaccacct gcagaaattc gattcgaagc gacaaccaac aggtgatacg
     1681 gcaatgataa cacgtcaaat gagccatcgc ccagcatggg acggtgtgca tgcaaaaccc
     1741 taaactccag aacctcacgg attactcccc ctcagacgcg ccctgggacg ctcacaaaag
     1801 cgtttctgac gacgtaggtg ggatttacct tctcgcagcc gaatatgagc gctacggggc
     1861 gcgtatggct tcctgtggcg gtttactgag gtttggttgg tctaccctca aagaaactgg
     1921 agaaactcgc cttag
//
