LOCUS       NZ_CP072542             4842 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli O128ac:H12 strain AW1.7 plasmid pAW1.7_3, complete
            sequence.
ACCESSION   NZ_CP072542
VERSION     NZ_CP072542.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18530112
            Assembly: GCF_018085225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O128ac:H12
  ORGANISM  Escherichia coli O128ac:H12
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Complete Closed Genome Sequence of Extremely Heat Resistant
            Escherichia coli AW1.7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Guragain,M. and Bosilevac,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2021) US Department of Agriculture, US Meat Animal
            Research Center, State Spur 18D, Clay Center, Nebraska 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-APR-2020
            Assembly Method :: Microbial Assembly v. SMRTanalysis v8.0; SKESA v.
            v 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 01:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,034
            CDSs (total)                      :: 4,915
            Genes (coding)                    :: 4,644
            CDSs (with protein)               :: 4,644
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 75 of 271
            Pseudo Genes (incomplete)         :: 170 of 271
            Pseudo Genes (internal stop)      :: 62 of 271
            Pseudo Genes (multiple problems)  :: 36 of 271
            Pseudo Genes (short protein)      :: 3 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072542.1.
            Bacteria and source DNA available from Joseph M. Bosilevac, US Meat
            Animal Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Escherichia coli O128ac:H12"
                     /mol_type="genomic DNA"
                     /strain="AW1.7"
                     /serotype="O128ac:H12"
                     /isolation_source="Cattle slaughter plant"
                     /db_xref="taxon:2822132"
                     /plasmid="pAW1.7_3"
                     /geo_loc_name="Canada: Lacombe"
                     /collection_date="2001"
                     /collected_by="Agriculture and Agri-Food Canada"
     gene            262..585
                     /locus_tag="J7886_RS24875"
                     /old_locus_tag="J7886_24870"
     CDS             262..585
                     /locus_tag="J7886_RS24875"
                     /old_locus_tag="J7886_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            794..>1228
                     /locus_tag="J7886_RS25340"
                     /pseudo=""
     CDS             794..>1228
                     /locus_tag="J7886_RS25340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1263..1754
                     /locus_tag="J7886_RS24885"
                     /old_locus_tag="J7886_24880"
     CDS             1263..1754
                     /locus_tag="J7886_RS24885"
                     /old_locus_tag="J7886_24880"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1761..1976
                     /locus_tag="J7886_RS24890"
                     /old_locus_tag="J7886_24885"
     CDS             1761..1976
                     /locus_tag="J7886_RS24890"
                     /old_locus_tag="J7886_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_137500696.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSYLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2334..3254
                     /locus_tag="J7886_RS24895"
                     /old_locus_tag="J7886_24890"
     CDS             2334..3254
                     /locus_tag="J7886_RS24895"
                     /old_locus_tag="J7886_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="WP_251364680.1"
                     /translation="MKFICRDRGGLPDGLLLVLADCRPVRGEAVRGGAGKGCYGKCGIF
                     YYMECIFMRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLYANSSYAM
                     KMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFVVSNDAVV
                     LATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYDKNGNYIG
                     HVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMVMSDDLKS
                     KLTESDRGEGAFFFRGIDIFWVMLI"
                     /name="cache domain-containing protein"
     gene            3251..4045
                     /locus_tag="J7886_RS25345"
     CDS             3251..4045
                     /locus_tag="J7886_RS25345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_251315947.1"
                     /translation="MKNTAWNVLVYSHADSTTTILITYAKSVLVLLIVIIITFSLLSYF
                     IASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRLKKALFTHVKVMMERVDSL
                     NEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFFKKINDSFGHGVGDEVLVS
                     LAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAERIRSVIERTVFPNNLRVTV
                     SAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     Typing          2..149
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.987"
                     /coverage="86.0"
                     /evalue="7.27e-67"
     Typing          complement(4342..4840)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.812"
                     /coverage="82.0"
                     /evalue="0.0"
     Typing          263..579
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.2e-61"
     Typing          576..2068
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtatcacat attctgctga cgctccgctg ctgcattttt ctcctgccac atgaagcact
       61 tcactgacac tcgcatccgt gccaacatag taagccagta tacactccgc tagcgctacg
      121 tgactggttc agggctgcgc cccgaaaccc gccaaaagcc tgacgcgcct gcggcttgtc
      181 caaccccgcg ccgaccggga aaggctttcc cgcccgtccc ggcgttatca ggaggccggt
      241 ttgtcagaga aacggaacaa gatgctcacc atgtgggtga cggaggacga gcaccggcgg
      301 ctgcttgagc gctgtgacgg caggcagctg gcggcgtgga tgcggcagac ctgcctggac
      361 gaaaagccgg cacgttccgg aaagctcccc tcaatctcgc cggcgctgct gcgccagctt
      421 gccggcatgg gcaataacct gaaccagata gcccgccgtg ttaacgccgg cggcggcacc
      481 ggccatgaca gggtacagat tgtggctgcg ctgatggcca tcgatgccgg gcttgagcgg
      541 ctgaggcacg ccgttcttga gaaaggaacg gacgatgatc gttaaatttc atccacgcgg
      601 tcgcggcggt ggcgccgggc cggtggacta tctgctgggt aaagaccggc agcgtgaagg
      661 tgccagcgtc ctgcagggga agccggaaga agtccgggag ctgattgacg cgtcgcctta
      721 cgtgaagaaa tacacatccg gcgtcctgtc gtttgcggag gccgatctgc ctcccggcca
      781 gcgggaaaaa ctgatggcca gcttcgagcg cgttctgatg cccggacttg ataaagatca
      841 gtacagcatt ctgtgggtgg agcacgcgga caaggggcgt ctggagctga actttctgat
      901 ccccaatacc gagctgctga ccggcaggcg gctccagccg tactacgacc gcgccgaccg
      961 gccgcgtatc gatgcctggc agaccatcgt gaacggcaga ctggggctgc acgacccgaa
     1021 cgcaccggaa aaccgccggg cgctggtcac gccgtccgga ctgccgaaaa cgaagcagga
     1081 ggccgcagaa gccatcacgc gcggactgct gacccttgct tcgtccgggg agctgaaaaa
     1141 ccgtcaggac gtcactgagg ccctggaacg gtcaggtttt gaggtggtgc gcaccacgaa
     1201 aagcagtatc agcattgccg acccggacgg ggggcgaaac atccgactga agggagccat
     1261 ctatgagcag tcttttaacg ctggcgaagg acttagagca gcaatcgaaa gcgcagaagc
     1321 agagtaccgg cgagatgctg aaagccgcat tcagcgagca cgagcagtct gtcagagcgg
     1381 aactgagcgc aagcgcgagg agaatcagcg acgccatcct cgcacacgag cagagtatga
     1441 gcgaggcgat ggagaaaaac cggcggagcg tactgcggac tgccgggcgg acatggctca
     1501 ccatcctgat ggtgtcagcg ctgctgatcg gcacgagcgg gagcatactg tggtggcagg
     1561 ggcagcagat aaccgacaat tacacgcatc ttcgtcagca ggaagatacc ctggcgaaaa
     1621 tgaccgcccg gacgtgggga gtgcgctatc aggagagcag cgacggccgg aggtttctta
     1681 tcctgccgcc ggggatgcaa accgaagcca tcccctacga cgggacgaca tggatccgcc
     1741 tgaaacagga gtagcggaaa atgacagagc tggaaaagca gttgctgagc gcattagagc
     1801 agctacagca ggactactcg caaaggctgg acgaatggga gagcgccttc gcggaatggc
     1861 ggagcatgtg tgggcttatg caacgggaga acgcggtgct gaacgagcgc gtgtcgtact
     1921 tgagcacgca ggtgctgagt ttaagcgagc agctgcgccg cttgtccggg aactgaatgc
     1981 catcgagctc cggcagaggg agaatgagta tcagaaaagc ctggagttgc agcgccagcc
     2041 gcagaaaacc tacaaaggac cgacgctggg ctgacgggtg ccgtcccgtg acaaacccca
     2101 agaagaaggg ctgcgaaatt ccccgctaaa tacgtcgtga tgcaaagatc actgcctata
     2161 ccagggggaa ttatgaaact gcttaaggtt attgaagctg tacttaactg cctgcatctg
     2221 gcggcagaac tgtgggagtg gctggagaag catttgccgg ttttcggggc cttcacgctg
     2281 tttttgggct gaatcctgcc gtcattcacc tggctgcatg gctatgcagt tgggtgaaat
     2341 ttatctgtcg cgatcggggt ggtttaccgg atgggttgtt gttggttttg gctgactgcc
     2401 gccccgttcg tggcgaagct gtccggggcg gtgcgggcaa gggatgttat ggtaaatgcg
     2461 gcatttttta ttatatggag tgtattttta tgagaattaa aaagcctcac cttcgtccga
     2521 tagtcataac cttgtccgtt ggtgggattt tgttaacttc tatttttctg attgccgtta
     2581 ttatggtttt tcagcgcgaa aatatagaaa aagatttgct ttatgctaat tcatcgtacg
     2641 ctatgaaaat gtcagatgta atgagtggtt atattgaaat ggctcaaggt gaattagcat
     2701 atggagcaaa aaaaataaaa tcaactaatg atattgactc acttaaagat gaggcggata
     2761 ggttaagatt gcagtctggt atgtttaatt cagtttttgt tgttagtaat gatgctgttg
     2821 ttctggcgac ttcgccagaa tctcttgatt tagttggtac ccgcttgaat tcaagtgcca
     2881 gcaaattggc gattgaggaa aagaaaccat tcatatcttc accgttcaaa tcagttactg
     2941 ggaatttgat tattttgttg tcacatccta tttatgataa gaatggcaat tacatagggc
     3001 atgtcggtgg gtctatatat ctaaaaaaac atagtttatt tagcgatgtt ttgagcaggc
     3061 attttttcaa gagcgacact gaaattagca ttgttagtga tgatggaact gtgatcttta
     3121 ataacgataa cagtgctgtt ggtcggccca tggttatgtc tgatgatttg aaatcaaagt
     3181 taacggagtc tgatagaggt gagggggcat ttttttttcg gggaatagat atcttctggg
     3241 ttatgctcat atgaaaaata ctgcttggaa tgtgttggtt tatagccatg ctgacagcac
     3301 gactactatt cttattactt atgctaaaag tgtacttgtt ctgctaattg taataattat
     3361 tacattttca cttctttcat atttcattgc ctcccgtatt tcacttcctt tagaaaagct
     3421 tgctatatct accaatgcta aagatatagg ggcttcgtta gtttatatta aagctatcaa
     3481 tgcttggtat gccgaagctg atcgacttaa aaaggcactt tttacgcatg ttaaggtaat
     3541 gatggaaagg gttgattctt taaatgaggt ggcgcttaaa gatcccctta ccggcgtgaa
     3601 caatagattg gggttttcaa ctaaaataca gaataacaag tatggggcag gagcttcagt
     3661 tattgctatt gatgttgatt tttttaaaaa aataaatgat agtttcgggc atggggtcgg
     3721 agatgaggtg cttgtttctt tggctcagat tattaagtct tgttgccgta gtgaagatat
     3781 tatttgtcgg tttggtggag aagagtttgt tgtttttctg ccgaatacat ctgtggtaac
     3841 agcggagtgt gttgcggagc gtattaggag tgttattgaa cgtactgttt tccctaataa
     3901 cttgcgtgtc accgtatctg ctggtgtggc aacacaagat gatcctcttg gtgggatcga
     3961 ttttttgctg aaaaatgctg acgatgctct ttatcaagct aagagagagg ggcgtaataa
     4021 agtgattgta tatagcgcta tttgagtttt tttgatgacg ctttacgcgc tagtgtgccg
     4081 ttttttttgt ggggaggggg gttcaagaag cggatcctct tgttatccct tcgatgtcgt
     4141 cgtaatctca tgccctgcaa acaaaaaaac caccgctacc aacggtggtt cgtttgccgg
     4201 aatcaagagc tacccactct ttcttccgaa ggtaactggc tttcacggag cgcaggtacc
     4261 aaaactgtcc ttccagtgta gccgcagtca ggccaccact tcaagaactc tgtagaacac
     4321 tctactactc cgctccgtga atcctcttac taatggctac cgccactggc gatttttcgt
     4381 gtctgttccg ggttggactc aagatgatag ttaccgggac aacgtggtag tcgggctgaa
     4441 cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcccggaa ccgagtgtca
     4501 ggcgtggaat gagataaacg cggccataac agcggattga catcggtaaa ccgaaaggca
     4561 ggaacaggag agcgcacgag ggagccacca gggggaaacg cctggtatct ttatagtcct
     4621 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     4681 agcctatgga aaaacggctt cgccgtggcc ttatcgcttc cctgttaagc ttcttcctgg
     4741 catctcccag gaaatttccg ccccgcctgt aagccggtac cgctcgccgc agccgaacga
     4801 ccgagcgcag cgagtcagtg agcgaggaag cggaatatat cc
//
