LOCUS       NZ_CP072499            10077 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN247 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP072499
VERSION     NZ_CP072499.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18450113
            Assembly: GCF_017743135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Zhao,Z., Lv,F., Luo,Y., Wang,W., Zi,C. and Yang,S.
  TITLE     hypervirulent Klebsiella pneumoniae KPN247 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Zhao,Z., Lv,F., Luo,Y., Wang,W., Zi,C. and Yang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2021) Department of Pathogenic Biology and
            Immunology, Guangdong Medical University, Xincheng Avenue, No. 1,
            Dong Guan, Guang Dong 523000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:15:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,421
            CDSs (total)                      :: 5,295
            Genes (coding)                    :: 5,168
            CDSs (with protein)               :: 5,168
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 68 of 127
            Pseudo Genes (incomplete)         :: 73 of 127
            Pseudo Genes (internal stop)      :: 26 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072499.1.
            Bacteria and source DNA available from Homo sapiens.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN247"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Zhanjiang"
                     /collection_date="2020-11-15T17:22:34Z"
     gene            complement(join(9337..10077,1..1221))
                     /locus_tag="J5253_RS26925"
                     /old_locus_tag="J5253_26920"
     CDS             complement(join(9337..10077,1..1221))
                     /locus_tag="J5253_RS26925"
                     /old_locus_tag="J5253_26920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_159210147.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2704..2853)
                     /locus_tag="J5253_RS26930"
                     /old_locus_tag="J5253_26925"
     CDS             complement(2704..2853)
                     /locus_tag="J5253_RS26930"
                     /old_locus_tag="J5253_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2938..3195)
                     /locus_tag="J5253_RS26935"
                     /old_locus_tag="J5253_26930"
     CDS             complement(2938..3195)
                     /locus_tag="J5253_RS26935"
                     /old_locus_tag="J5253_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3205..4890)
                     /locus_tag="J5253_RS26940"
                     /old_locus_tag="J5253_26935"
     CDS             complement(3205..4890)
                     /locus_tag="J5253_RS26940"
                     /old_locus_tag="J5253_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5217..5462
                     /locus_tag="J5253_RS26945"
                     /old_locus_tag="J5253_26940"
     CDS             5217..5462
                     /locus_tag="J5253_RS26945"
                     /old_locus_tag="J5253_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159210150.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKEVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            5736..6107
                     /gene="tolA"
                     /locus_tag="J5253_RS26950"
                     /old_locus_tag="J5253_26945"
     CDS             5736..6107
                     /gene="tolA"
                     /locus_tag="J5253_RS26950"
                     /old_locus_tag="J5253_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6104..6322
                     /locus_tag="J5253_RS26955"
                     /old_locus_tag="J5253_26950"
     CDS             6104..6322
                     /locus_tag="J5253_RS26955"
                     /old_locus_tag="J5253_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            6664..6942
                     /locus_tag="J5253_RS26960"
                     /old_locus_tag="J5253_26955"
     CDS             6664..6942
                     /locus_tag="J5253_RS26960"
                     /old_locus_tag="J5253_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6930..7244
                     /locus_tag="J5253_RS26965"
                     /old_locus_tag="J5253_26960"
     CDS             6930..7244
                     /locus_tag="J5253_RS26965"
                     /old_locus_tag="J5253_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7408..7836
                     /locus_tag="J5253_RS26970"
                     /old_locus_tag="J5253_26965"
     CDS             7408..7836
                     /locus_tag="J5253_RS26970"
                     /old_locus_tag="J5253_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7862..8041
                     /locus_tag="J5253_RS26975"
                     /old_locus_tag="J5253_26970"
     CDS             7862..8041
                     /locus_tag="J5253_RS26975"
                     /old_locus_tag="J5253_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8069..8599)
                     /locus_tag="J5253_RS26980"
                     /old_locus_tag="J5253_26975"
     CDS             complement(8069..8599)
                     /locus_tag="J5253_RS26980"
                     /old_locus_tag="J5253_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159210149.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8606..9337)
                     /gene="mobC"
                     /locus_tag="J5253_RS26985"
                     /old_locus_tag="J5253_26980"
     CDS             complement(8606..9337)
                     /gene="mobC"
                     /locus_tag="J5253_RS26985"
                     /old_locus_tag="J5253_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_159210148.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEGRGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKVNGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9890
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2023..2601
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8610..9337)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="9.26e-141"
ORIGIN
        1 caggtcggcc acgttctcca gcttggtgat cagccccttc acgtcctttt tcagggcctc
       61 cggcagtgcg tacgccgccg ccagcacctg ctgaatattt gccccctgcg gaaactgctc
      121 cgcaatcagc ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt
      181 ctcactcagg ttaaatccgg caaccagaag attcttcagg gcgtactggt cgagatcgcg
      241 gaacagattc agctgcgccg gtttccccgg ccgcagatcc agcagggtga agtccgggca
      301 gtgcgcccgg aagacgtgcg gcgcccacgc gtcccctttc gggtcgaagt acaccaccgc
      361 gtccccgaaa tcgcgtacgc agcgaatgag cgcgttggtg gccatcacgc tcttgccgct
      421 cccgggcagg ccgaggatct gaatgttggt cttgcgccag gtatcacgcg ggaccagcac
      481 cggctggccc tgctcatcct tacccagaaa caccgcctgc tgtttccggg cctgctccgt
      541 ggaaaaatcg gccagcgcgt taaccggtac cggctccccg aaatgcaggt ggcggacgtc
      601 ggtcatttct ccggtccgct ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac
      661 cgcctcgtca gcggccgcaa aatgcccgtc aagcagcagg aacgccagca gccgcgccag
      721 gaggacgcct gcgacaagac ctccggcaca ccaggcaatc ccgaacaggt tagccggctg
      781 cagcaggtaa taatctgcgt tcgcgtagag gtccggacgc aggacggttt ctccccagct
      841 ctcgataaac acggcgacaa ccagcccccc cagaagggcc agaccggcag cgccggtcag
      901 ccagtatgcg tagttccaga catgtcgccg tgccctgacc atccgcccgg gcgaatccag
      961 cgaggccagc accgaaaatg gcgcagtccg gttattatcg cgcttatagc gatcggcata
     1021 ctcccgccgc tccttatgac tcagccccac gaatcgcatc cgcccggccc agagttctgc
     1081 cagaccgaaa aacaggccga taaccagaaa aaaaccggcc agactgtaat actcccggta
     1141 cagccagctg acccacgggt aaatctgccg ggccagctcc gggatatgcc actgccaggc
     1201 aagcgaatcc gtattaaaca tgcaatctct ctccttcaga cgacgggttt accggcgtgt
     1261 tgtgcagccc ctgctgcaca agccgcggac atgccccaag gcatggcggc ggcggtcacg
     1321 tccgtggtga ttgcgttcag ctttcaccgc ggaggccagc ctctttccgc cccatgcgga
     1381 aaaggcgcag ccccggagga acaacgagca cgccccagcg tgtgagagga aaaggcggca
     1441 ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga gttcgccata gcgagcggct
     1501 gaccacccgc aaaatcagga gcagctttgc tgcttcgtca ggtgcgtcca gcaccgtgga
     1561 ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtgaccggc
     1621 gtccagccgg acagagccag aaagaggggg aaagacagtg cgtcggtggc gtccagccag
     1681 cgtaagcagg ggatttcccc ctgaagtcag tcacgcccca gcggggatga cgtgcattcc
     1741 tccgcaagga gaaaaaagca acagagatag gttaacgtaa aatattcagc agaaaaccga
     1801 cagacttcac ctatctgaat aatttatcgg cagggaagag aaaggcggta acacggagca
     1861 atatgtcaca tccacgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct
     1921 cgctcactcg gtcgctacgc gccggccgtg agactgcggc gggcgctgcg aactctcgca
     1981 aagttaccca cagattccgt ggataggcag ggaacgagca tgtgaggcaa aacggcaggg
     2041 ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac
     2101 gctttttcgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg
     2161 aaacccgaca ggacttaaag atccccaccg tttccggtgg gtcgctccct cttgcgctct
     2221 cctgttccga ccctgccgtt taccggatac ctgtccgccg ttctcccttg cgggaagtgt
     2281 ggcgctttct catagctcac acactggtat ctcggcccgg tgtaggtcgt tcgctccaag
     2341 ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt
     2401 tcacttgagt ccaacccggg aaagcacgca aaacgccact ggcagcagcc attggtaact
     2461 gggagttcgc agaggatttg tttagctaaa cacgaggttg ctcttgaagt gtgcgccaaa
     2521 gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg
     2581 gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt
     2641 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2701 tttctactga accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc
     2761 ctgaacatcc cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa
     2821 tataaaaaga aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg
     2881 gcattcatcc ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta
     2941 ccagttaccg tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg
     3001 atttttaaaa aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt
     3061 attaatatta tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa
     3121 gtctttagaa tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg
     3181 taatttaagc cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg
     3241 ccctttaacc tgcttacccg ttttgggatc aaatgccccg aggtgttcgc catcactggc
     3301 acgataccct tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc
     3361 gccataccat cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg
     3421 ctttaactct ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc
     3481 gtagtcttta gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc
     3541 cttcgctttt ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc
     3601 tctggataag gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt
     3661 ctgattctga atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc
     3721 atgaggcggg ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt
     3781 atcggcttcc tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg
     3841 gctgtttaag gtcgcaatat ttttatcttc ggcatccagc tcagctttcg ctttatcata
     3901 gtctcttttc agcccctctt ccggatgagc ggcgtcccat gcctgctggc gacgcttttc
     3961 ttcctcctga cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg acacataaac
     4021 cggcttctgt ccggtctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc
     4081 tgtaaaacca gccggacgtg aatcaccttt ttcagcaaca atgccttttg gcggggcttt
     4141 cgctgtcgga acacctttcg gtacgctcac ctgcagagac gggagacccg gaatcgacac
     4201 actgaatacc cccggacgtt ttgtcggttt cgcatccacc acagggacac tcattggccg
     4261 acctgcgaca accgcaatat gctgccgctc atccttcacc acatccacaa cccgttgtct
     4321 gacacgaacc gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc
     4381 ggccggtaat gacgtcataa tttttgacat cattttcgga tcatcttttg caatctcaga
     4441 aggcagcaca ccgtagatcg caatccccca cagaccaaac ttaaacggcc ccttcagggc
     4501 agccagcaca tcagcaacgg cggccgacaa cgcatcgccg gaaactgata aagccagtcc
     4561 ttcagccccc ggagtagcca gggcaggcag accgaaggcc atcgcagtgg ccgaagactg
     4621 tccgccacca gagctaccgc tatgattgcc gctgttacca ccattaccac cgggtttact
     4681 cccgccgtta ccaaactccc ccggattata gttatgaata tctccattcg gcgtatgcgt
     4741 cgtaccccag cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt
     4801 accagacgta ccgttcacat tcccactggc ctgaccacca ttatgtccca gacctgaatt
     4861 acccggacct cgaccgtctc caccactcat atcgttttcc tctttgacat ttaaaacaat
     4921 aaattaaagc caacatactg tacatataca cagtataatt attaccttac tcctcccgtt
     4981 ttcaggtgtc aatatatgag attagttctc atcttcaggc acaaaaaagc gcccggagcc
     5041 acctgtaagc agatcgcgtc ttttcccgaa aatttctcac ggcaacaaat tttattattt
     5101 gagatgtaca tcacacttac atttcaggtg gtatagacac atctgaaagc taactggtat
     5161 gaggggagga cccgcggtta aactgttcag gaaacctgag acgcccggag gccaccatga
     5221 cacacctgaa actgactccc gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg
     5281 cactggccat gggcctgaaa gggaacagaa cgctgccaaa ggaggtaaaa cagcaggtgc
     5341 gggaaagggt gcttcgcgcc attcaggacg aacgtgaagc caggcgggcg caggaaatgc
     5401 gtcgggagaa atacgaggcg gcggaaacca ccttcacctg gcagccgctg cgccgccggt
     5461 gaggggtatt taccataaaa cccgttatca gcaccgcccg agaagccctc agagcgtttc
     5521 cggcggctac gggataaaac aggcaacaaa tcacccggaa aaccgccgga aacgcgcccg
     5581 gtaaattgta acccgctgca tgcctatgca cgcaactgca tggcgggccg tttggtgtaa
     5641 aaacgatacg ataagccgtg tttatgtcaa tgaacgtggc cgtaaagagt ggcaaatata
     5701 catgtaatat tttttactga caggtcatca acccgatgcg tttaataccc tttatcctgc
     5761 tggcattgtg cagcccctta ctcctgacgg cctgtaagca ttccaccacc cagccgccga
     5821 aaatttatac tctggataag tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg
     5881 ctgttgaaat gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga
     5941 tatccataca acgtgatggc actctgaaca gcgccattgc tgaaggagga gatccgaaac
     6001 tctgtaaagc agcaatatca gccgttaccc acgccaaaat tccgccggca ccggatgaaa
     6061 atacctggca aatattcaaa aacgcccctc tggatttcag accatgaaag cttttactct
     6121 gacagtgctg gcggccatgt tcctcactcc ggtacatgcg ataccactaa aacccgtaac
     6181 cacactgcag ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac
     6241 cgccacgttt gatattctgg acgatggacg agtggacaac gtgtcgctta ccggctgtca
     6301 cttttttttc gggaaacgct gacggcaatg aggcactggc tatatcccgc agggcacccg
     6361 gcaatgcgac aggttataaa ttttgaattc agtctgaaaa atgacggctt acaggatccg
     6421 cgaccagcca ccaccaaaaa gttcaatgat gatggttgcc gccagtaaca ggtcctttaa
     6481 cccgcaggct gcaatagtcc tggatgatga gtgagcctgt agcagccctg attagtggaa
     6541 gcgaagctga aaaatgggga agcgaagctg aaaaatgggt agcaaaagcc gtatttctgg
     6601 caaatagcgc cgccacggat taaagtagtc atatgtatta cacatgacta caaaggggct
     6661 tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg gctgaaaacg
     6721 ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat caccgaatac
     6781 atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaagccgc atggcagcac
     6841 tatcaggaaa cgggtctgca ccttactgcg gaagaagcag aagcctggat aagtacctgg
     6901 ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc gcgctggcac
     6961 agcgagattt acaacgcctc caggactttc tcaaaaccaa aaatcggctg gcagccagaa
     7021 aagccggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca gacattggcc
     7081 gcccggtgcc atttctgccg ctggaatatc aggaactggt gatcgggttt ggcgacagtg
     7141 gctatgtgat gctctaccgc cacgaccggg aaatggaccg gattgtgatc gtcaccgtca
     7201 ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg atgacggggg
     7261 cgcaattcat tgcgtggccg tcgccggttt gacaagcaca gcgcgtcaga aaaccacatc
     7321 ctgaacggaa aaacgggtaa aattcagcga tacatttacc gtgagaaatt acacaccttt
     7381 cacacccgat aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct ttcacaggca
     7441 gcacaagccg cgggcattac cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt
     7501 accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact gatccgtgta
     7561 tacggaaatg tgaatatccc tgagaaacaa attcccggca tttcacaccg tgaaaataca
     7621 caaaaaaatt tcccacagga acagcttctg gcaatgcaaa aggaactggc cgatctccgg
     7681 caggctgtca ccctgatgct ggaagataaa acatcccgag aagaagaacg ccgccagcac
     7741 gatgatgagc gccggaaact ccaggccgaa gtggacagat tgacaacaga actgacacag
     7801 aagaaaaaac gcttctggtc cggctggttt agctgagaac ttaccgaagg gaatggagaa
     7861 catgacgaag caggaaaaaa ccgccctcaa tatggctaaa ttcattaagg accagtcgct
     7921 gctgttgctc gagaaactga acgagctgga tatggatacc ccggccgaca tgtgcgagcg
     7981 gctgcacgag gatgcggaaa cactctggca ggtgatgcag gataagctcg gggaagaata
     8041 acagcaccgg catggccggt gctggcgttt attttttgcc gaacggccac ctccggcggg
     8101 cgggtttatc ctccggcagt tctggcaccg gaacgttaaa atagcggctg ttatattccc
     8161 gcatcagctc cgctttcagc ttctcgttca ccgcgctttc cccgtaccgc tcactcacga
     8221 cgtcaatcat cgccccgatc tgctccgggg tgtatttccc ggcgtccagt acctgcacgc
     8281 cgttctcgat ggcgaactgc tcaaccttgc gataaagata atcacacagc ttcattccgg
     8341 ccggtttctc acgttcccac gccggatttt tgccggtgaa gtaatacgtg gctttgtcat
     8401 cgctgttcag ccgttcgtga tacatgccga taaacttcat cattgccaga tgctgggcct
     8461 gatcttcaaa cctgaataca tatgcaccgg tctgccaggc catgaccgcc atgtcttcca
     8521 gatgctcgct ggttggcata atgcagcgct cgccgtcccg gtcctccgcg tcttcaataa
     8581 ataccatgta cagcgccata atccctcact ccgttgtcat aaatttaaag catgcgaaca
     8641 gcctctcccg gtccagctcc gccgttttgc gggcctcgcc ggtctgcggg ttcacgattt
     8701 catccaccgc cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca gggcagatgt
     8761 accagatttc atcgatgccg ttcaccttac ggttgaacag atacccggac accacttcct
     8821 gataccggcg acggctcttg acggtacgtt ccacctcaaa cgccaccgtt ctgccgtcca
     8881 gctccgccag cgcgtccggc accttcatcc ctttcagggc aatacggtgc aggtaccgcc
     8941 agccggtggc cccccggccc tccatggcga ggcgcaggga ctgcacggcc agccggtgcg
     9001 gcagctgcgc cgccgacacc cgcgacggct gaaacggaat aatgtcagtc agcggctcgt
     9061 cctcgtcgaa cgagaacgcc accccgtgcg gcgtcagccc ccacacgctg atctgacggc
     9121 cgtcaaccgg gtatttcgcc cggaccagca gccctttcgc caccaggctg tccaggaagc
     9181 ggtaaaccgg ctgtaccgcc gcaatcccca gacgtcgcgc aaccagctcc gccgtggaac
     9241 acagctcttc ccgcagccag cgcagggtca gccgtgtttt ctccgcgttc cgcgcctgcc
     9301 gcttcgcgtg gctgacgttg tatcgctcca gtgccatcag tacagccccg caaaatcatc
     9361 atcacccggc cgctgccgcc cggcctccgg ctggtactca gccagcgggt ccgttttcgc
     9421 cagcaccggg aaggttttca gaggaatttc ccggcgatca acacgcagcg ggctgctgaa
     9481 cgccagccgc gccacgccca gcccggtcat caccgcgaac ccgtcaggca gatgttgcag
     9541 ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtccc cgctgatatg
     9601 ctccacgttc cccgcctccc ggctggcccg gcggcgctcc acgtccaccc ggatctcccc
     9661 ggtctgtccg gccgcccact gagcggactc ctggctggcc gcccggtaga accagcggat
     9721 cggcgtgtta tccagtaccg tggttttcac aaaatcagcg ggcagatcct gcccgcactg
     9781 gccgaagtcg cccagcgact ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt
     9841 ccccagcgcg ttcagcacgt acttcgacag cagatattta atctcatcga gcatgatgct
     9901 cgcgtgcggc cacctccgga actcgtcccg cgcaatgatg atttgcgcgc agcgggcaaa
     9961 cagcatcttc tgcacccgga tcaccgcctc atcgtccatc gagccgatga cgtacaggca
    10021 gccgccgccg ttgattatcc ccgccacgtc gatgccgctg tccgtctgaa ggacgct
//
