LOCUS       NZ_CP072459             2460 bp    DNA     circular CON 21-JAN-2024
DEFINITION  Klebsiella pneumoniae strain b1-2L plasmid pb1-2L-3, complete
            sequence.
ACCESSION   NZ_CP072459
VERSION     NZ_CP072459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18487814
            Assembly: GCF_020172745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,J., Wang,J., Yang,H., Su,M., Li,R., Bai,L., Feng,J., Huang,Y.,
            Yang,Z. and Tang,B.
  TITLE     IncHI1 plasmids mediated the tet(X4) gene spread in
            Enterobacteriaceae in porcine
  JOURNAL   Front Microbiol 14, 1128905 (2023)
   PUBMED   37065147
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Ma,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2021) College of Veterinary Medicine, Northwest
            A&F University, No.3, Xianyang 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/21/2024 09:47:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,530
            Genes (coding)                    :: 5,396
            CDSs (with protein)               :: 5,396
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 79 of 134
            Pseudo Genes (internal stop)      :: 16 of 134
            Pseudo Genes (multiple problems)  :: 17 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072459.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="b1-2L"
                     /host="swine"
                     /db_xref="taxon:573"
                     /plasmid="pb1-2L-3"
                     /geo_loc_name="China:Guangxi"
                     /collection_date="2019-05-11"
     gene            complement(join(2210..2460,1..232))
                     /locus_tag="J7V71_RS28230"
                     /old_locus_tag="J7V71_28230"
     CDS             complement(join(2210..2460,1..232))
                     /locus_tag="J7V71_RS28230"
                     /old_locus_tag="J7V71_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202974839.1"
                     /translation="MNTKKISKLERHTYMDESGEIKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFLRAGRGELEANPFLFARGDWPSIYERRKKIELRISYDSNGRTVQTTVTDDER"
                     /name="hypothetical protein"
     gene            926..1894
                     /gene="mobV"
                     /locus_tag="J7V71_RS28235"
                     /old_locus_tag="J7V71_28235"
     CDS             926..1894
                     /gene="mobV"
                     /locus_tag="J7V71_RS28235"
                     /old_locus_tag="J7V71_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1993..2223)
                     /locus_tag="J7V71_RS28240"
                     /old_locus_tag="J7V71_28240"
     CDS             complement(1993..2223)
                     /locus_tag="J7V71_RS28240"
                     /old_locus_tag="J7V71_28240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029488008.1"
                     /translation="MMNAEDKLFKKVDRLMLHDSLKKNEVLTVWERTFLSSTYSIYRRT
                     SGNEFSTKGLSPKQKSTAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     Typing          918..1891
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tttttgatac agatacaaag ccatcccatc caatcagcat tgccaactgc aaaagcacat
       61 cattttgaga ttttgacaaa ccatgaatca tcgcaatgtc tgcgatgtaa agcttgacgt
      121 agggcggttc agccgggata gaaaaagtct cgatcttaga gtcttgttta atctctccgc
      181 tctcgtccat gtatgtatgc cgttcaagct tagaaatctt ttttgtattc actctagccc
      241 catgtatcag catgttttta gtactcacat ttaagtacta aagatgccgg tagtcaagta
      301 ccttacctaa agtacgatta gtacttgagg tgagtacgat tagtacttga ggtaagtacg
      361 attagtactt gaggtaagta cgattagtac ttgaggtgag tacgattctt ctctctagcc
      421 cttggtgtgc gcgggttccc gtatctatat ctatatatta agggggaaaa taaaggctga
      481 aaaacgccat ttttaaacat cgaaaaacga gcttttttga gtctggagcg tacggatttc
      541 tatgtccggg gagtggaaat tccgcttcct cgaacggaca gactcgcggc gatgccgctc
      601 cctaatccgt tcttgcggcg agcggttcct gctctttcaa aaacgatatt cattgcctga
      661 tttcgtcaag ggcgagcgta gcgagtgcca tttacccttg acgactcacc ggataaaaca
      721 ggcttggcag tggtgggagg tgacgcggag cgacgccttc cacctgccag accgccgttg
      781 attttgacct ttggaagcgc agcttcccga acgattttgc gaagcaaagt ctagtgagta
      841 cactatcatc ttgatagtgg cccgccggag gtatcgcaat gcgctccccc gcctggctga
      901 atgaatgccg gaatcagggg gggacatggc gaactacgcg ataatgcgct gcaaaaagct
      961 gacgggaatg ggaagcgtgg ccagcgcgtt gcagcactgc taccgagagc gtgagacgcc
     1021 gaacgctaac gctgaacgga caccagaaaa ctactgctct gtgtccaaat caacggatca
     1081 ggcaatgggg cgtgtgcggg agctgctgcc ggaaaaacgc cgtaaagatg cggtactggc
     1141 cgttgaatac gttatgacag caagccctga gtggtggaaa gaggccacac cacggcagca
     1201 ggcggagttt ttcgcccgct ctgagcaatg gctggagaag aaatacggga aggatcgtgt
     1261 tgtggcggcg gtggttcacc gggacgaggc caccccgcac ctgtctgcgt tcgtggtgcc
     1321 attgacgcag gatggacggc tgtccgcgaa agagtttatt ggcgggcggt caaagatgcg
     1381 tgaagatcag agcacctacg ctgaatcagt aaaaaaactg ggtcttgagc gcggcatcga
     1441 gggaagccgc gccacgcatc agacagttca gcactactac gaatcgataa accgtggcac
     1501 ccgtagccag gtatcgatct cgccggaggc gctggagccg cgtgtattgc ggaagggcat
     1561 tttcaccaaa gacgtggaag atcaggcagc catagccaaa cgtctaagcc acgcagtgaa
     1621 cgatggcttt gcaggaacca tcgctatggc ctctcagagc gcccagaacg caaaaagagc
     1681 gcgtgagcta cagaagacta tggatgcgca gcagaaacgc ctacagagcg ttacagagcc
     1741 ttttaagggg ctttccagag agcagatgac cgaaatcctg atgatggctc agcggtttaa
     1801 gcagcaaaac caggaaaagg aaaaacagca gcgtattgaa cgtgaaaaac agcgacagac
     1861 gcgatcgcgt ggcatgggtg gtatggagcg ttagttttga tgcgatgcag gatagggcta
     1921 acgccgcaaa gtgacgtcat ttgcggcatt ccagagatca ggttctgccg ccctgtataa
     1981 cgctgagtcc agctaatcca tcagatggta attgtatttt ttcaaaatag aaaaagcggt
     2041 gcttttttgt tttggtgaca atcccttagt tgaaaattca ttaccgcttg ttctgcgata
     2101 tatgctgtat gtactactta aaaaagtacg ctcccaaaca gtcaaaacct cattcttttt
     2161 tagagagtca tgcagcatta atctatctac ttttttgaat agcttatctt cagcgttcat
     2221 catccgttac cgttgtttgt actgtccttc cattggaatc ataactgatt cttaactcta
     2281 ttttttttct tctttcgtaa attgaaggcc agtctccccg agcaaaaaga aacggattgg
     2341 cttcaagctc tccccgccct gcccggagaa agatttgttt atcaacaagg gcagcgatta
     2401 tgtttttaaa cgtctgatat tcaatatgaa ccctaggttg tatggtcttc tcaaatctag
//
