LOCUS       NZ_CP072420             2731 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B32278 plasmid p7B32278, complete
            sequence.
ACCESSION   NZ_CP072420
VERSION     NZ_CP072420.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18478623
            Assembly: GCF_017742935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Complete genome sequencing of K. pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler Hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,413
            CDSs (total)                      :: 5,285
            Genes (coding)                    :: 5,141
            CDSs (with protein)               :: 5,141
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 63 of 144
            Pseudo Genes (incomplete)         :: 82 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 20 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072420.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B32278"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p7B32278"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2014"
                     /collected_by="Christian Medical College"
     gene            join(2693..2731,1..144)
                     /locus_tag="J6256_RS26900"
                     /old_locus_tag="J6256_26895"
     CDS             join(2693..2731,1..144)
                     /locus_tag="J6256_RS26900"
                     /old_locus_tag="J6256_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_209167494.1"
                     /translation="MCPFSGHLPHRYENQSLLLLEKLNELDLDVEADLCEKLHDDAEHL
                     FRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            144..566
                     /locus_tag="J6256_RS26905"
                     /old_locus_tag="J6256_26900"
     CDS             144..566
                     /locus_tag="J6256_RS26905"
                     /old_locus_tag="J6256_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(759..1619)
                     /locus_tag="J6256_RS26910"
                     /old_locus_tag="J6256_26905"
     CDS             complement(759..1619)
                     /locus_tag="J6256_RS26910"
                     /old_locus_tag="J6256_26905"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(1802..2359)
                     /locus_tag="J6256_RS26915"
                     /old_locus_tag="J6256_26910"
     CDS             complement(1802..2359)
                     /locus_tag="J6256_RS26915"
                     /old_locus_tag="J6256_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            2523..>2729
                     /locus_tag="J6256_RS26920"
                     /old_locus_tag="J6256_26915"
                     /pseudo=""
     CDS             2523..>2729
                     /locus_tag="J6256_RS26920"
                     /old_locus_tag="J6256_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     ARG             complement(763..1619)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aatcagtctc tgctcctgct ggagaagctt aacgagcttg atcttgatgt agaggccgac
       61 ctttgtgaga aactccacga tgatgcggag catcttttcc gtacactgtc ttcgggactg
      121 gatgaattac aggatggaaa ctgatggcca gagtgtctat tagtgaagcg gctcggcttg
      181 ttaaggttag tcgccccaca atttacaaga tgattaacag tgggaaacta agttacacct
      241 ctgttgtaaa gcatgggaaa aatactaaag ttgttgatac atcagagctt attagggttt
      301 ttggctcgct ttatggtggg ggcgacactg taaaacatga tgtaaaaggt gacgcaggcg
      361 ctacaggggt taacatctct ggtttacatg atttacagca taaaattgct ttgcttgaag
      421 ctgaaaacga tggtcttaaa ggggctgtta aagctcgtga tgaacatatc gattctcttc
      481 gccaggctat gcagcttctg gaacacaagc tcgaaccctc tccacaggtt cattctccgt
      541 ggtggaaatt ctggaagaaa tcctgacccc gtcatgcttc agttttcctc tccggccatt
      601 ccggggagtt cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
      961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     1081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1201 atcgttgtca gaagtaagtt ggcagcagtg ttatcactca tggttatggc agcactgcat
     1261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     1381 gataataccg caccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     1501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac gaaaatgttg aatactcata
     1621 ctcttccttt ttcaatatta ttgaagcatt taccagggtt attgtctcat gagcggatac
     1681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1741 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1801 atcacgaggc cctttcgtct tcaagaattt tataaaccgt ggagcgggca atactgagct
     1861 gatgagcaat ttccgttgca ccagtgccct tctgatgaag cgtcagcacg acgttcctgt
     1921 ccacggtacg cctgcggcca aatttgattc ctttcagctt tgcttcctgt cggccctcat
     1981 tcgtgcgttc taggatcctc cggcgttcag cctgtgccac agccgacagg atggtgacca
     2041 ccatttgccc catatcaccg tcggtactga tcccgtcatc aatgaaccgg actgccacgc
     2101 cctgagcgtc aaattccttt atcagttgga tcatatcggc agtgtcgcgg ccaagacggt
     2161 cgagcttctt aaccagaatg acatcacctt cctccacctt catcctcagc aaatccagcc
     2221 cttcccggtc tgttgaactg ccggatgcct tatcggtaaa tatacggttt gctttcacac
     2281 ctgcgtcttt gagtgctctg acctgaagat caagagactg ctgactggtt gagacccgag
     2341 cgtaaccaaa aagtcgcata aaaatgtacc ttaaatcgaa tatcggacaa ctcatgtcta
     2401 ttattacaaa tttacgattt aatagacata ttaatgtaac agttttacga tgtccgataa
     2461 tttataacat ttcgtacggt tggaaaaatg ttactaaatg cccgtcaggc agggaggccg
     2521 atatgcccgt tgactttctg accactgagc agactgaaag ctatggcaga ttcaccggtg
     2581 aaccggatga gcttcagctg gcacgatatt ttcaccttga tgaagcagac aaggaattta
     2641 tcggaaaaag cagaggtgat cacaaccgtc tgggcattgc cctgcaaatt ggatgtgtcc
     2701 gttttctggg caccttcctc accgatatga a
//
