LOCUS       NZ_CP072417             3998 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B32278 plasmid p4B32278, complete
            sequence.
ACCESSION   NZ_CP072417
VERSION     NZ_CP072417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18478623
            Assembly: GCF_017742935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Complete genome sequencing of K. pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler Hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,413
            CDSs (total)                      :: 5,285
            Genes (coding)                    :: 5,141
            CDSs (with protein)               :: 5,141
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 63 of 144
            Pseudo Genes (incomplete)         :: 82 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 20 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B32278"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4B32278"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2014"
                     /collected_by="Christian Medical College"
     gene            complement(join(3971..3998,1..236))
                     /locus_tag="J6256_RS26850"
                     /old_locus_tag="J6256_26845"
     CDS             complement(join(3971..3998,1..236))
                     /locus_tag="J6256_RS26850"
                     /old_locus_tag="J6256_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(233..1087)
                     /locus_tag="J6256_RS26855"
                     /old_locus_tag="J6256_26850"
     CDS             complement(233..1087)
                     /locus_tag="J6256_RS26855"
                     /old_locus_tag="J6256_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            complement(1624..2817)
                     /gene="mobQ"
                     /locus_tag="J6256_RS26860"
                     /old_locus_tag="J6256_26855"
     CDS             complement(1624..2817)
                     /gene="mobQ"
                     /locus_tag="J6256_RS26860"
                     /old_locus_tag="J6256_26855"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_096645250.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQIEWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            3061..3444
                     /locus_tag="J6256_RS26865"
                     /old_locus_tag="J6256_26860"
     CDS             3061..3444
                     /locus_tag="J6256_RS26865"
                     /old_locus_tag="J6256_26860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            3448..3732
                     /locus_tag="J6256_RS26870"
                     /old_locus_tag="J6256_26865"
     CDS             3448..3732
                     /locus_tag="J6256_RS26870"
                     /old_locus_tag="J6256_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     Typing          complement(1808..2817)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.703"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 gcaacataac ggcgatacag ggcaacggct ccgtgatacg aacagcccat tttgtcggct
       61 acttgttgcc atttcagccc ctggcttcgt agttcgtaag cctgacgacg acgatccatt
      121 gctgtttttt cgtaatcgtc acgagattga gaaaaatatt ttcttacagt tctttcacta
      181 cgacctgtca tttccattat ttcttttgct tttatttttt taccattaat gatcataaaa
      241 cccccttgct cattaatatg tctaacattt catttttaac attggttttt cgaggttttt
      301 tggctctgtt tgattggatt tgtgaaaatg tatggctatc aaaatttttc caagtccaac
      361 gggctacact tctaactgtt gcttttatct ctttgtattg taacgggttt tgtggattgc
      421 aaaaagtgtt taagccctgt gcctttaata gtagttcgtt ttcgaatttc tcgaaagtgg
      481 atattttttt gaaatttaaa accgttctgt aagcatattg ccgtagatct tcaaacaaat
      541 taacattgcg acctaaacca taagcctcgc tttttttctt ttgtatttga ggcttttcta
      601 agtccacaaa atcagctaaa taatccaggt cataaaggtc agcacctgac cagaacggcg
      661 accagtgatc attgagtggg ttttttgtaa tcaatccagc aaaccccaga tctgctttta
      721 gttttttcgc catagccatt tcaacggctg cggcatactt caacggcttt aaccttgctt
      781 gttgtgtctt acaaaccccg gctttcagca tatagccgta gtgacaacga cctgtttttg
      841 gtgtttttgt aataaagtga ggtatgggta agcccacctg ctcaaatgta tagaatgaat
      901 ttttcttatc tagatcaaag agtaagcaag tttgaatggc tggctgattt gcctgtaaat
      961 acagcatttc aagggctttt gccttgttcc ttatgcttaa tcccatatcg aggttatttg
     1021 tacaataagg cttgttaggc atttgatttt taaaaaaatc gatgttatgt tcttcttgta
     1081 ttatcatttt ttcagtcctt tttatctggc cctactctct gttttctgtc gccaaaacaa
     1141 aatcgcagag ggtggggttt ttatttttat agatttttca atcctgtacc agttctaacc
     1201 gatctcaaaa atttgtcaag tgtgcgtcta accatatcag ataacagccc aacgaagttg
     1261 gggcagttct ttacatacgg cagcaaagca ttactatcag atctatggtg cgaagcatta
     1321 ccgaagatag aaaaacaata aaaaaagaaa aaagagtatt gttcgaagaa aaattggcga
     1381 ttcgattttt tttggtgaat atatgaaaaa aaagaaagtg cagaaaaaac ggaaaagaaa
     1441 taagcatagt aaatttttta aaatgcttat gaagtcagtt aatgaagctg tggcaataca
     1501 taaaggggaa atagaaccgg cgaggatcac aactctaaaa atgtagtatc ttatagtata
     1561 gtattaaata atattaagtt gtattaagtg atactactat ataggaatat ataccactat
     1621 atactacatt ccaaatttct tcttcctctt aggttgctga tcttcgaatt ccggcattgg
     1681 ttccggctca ggctcttcaa tttttggttg tggttggttc ataggagaca ttccagagcc
     1741 tgaacccatt tttccgcctc cattatttcc accggttccg gttcctgtgc ctgttttagg
     1801 ttttgtttct aactctgtct ttaatatatg ttcgccaaat tcatttatta cataactact
     1861 aacgaaattt tgaggatctt ctttttcttc ttttttattt ttatttacat taggttttga
     1921 tttaaatata tgctgggtct ttttgtataa tgcctttaaa ttatcaatga ttgaccttgc
     1981 aagaggcttt ttctctattt tcatagtttc ctctggctga ggtggtttgg tttgctgtaa
     2041 gtatgttgca ttagctattt ttaataataa tgctttttct ttttgctcca tagctcttaa
     2101 tttttgacct tttcgccatt ggtgcctgtg tatcctggtc attggcgttc tgtccagttc
     2161 aatagttttt gaaatccact tggctttctc tttcggagtt ttagcaaaaa cagcctttct
     2221 ttctgcctct tctttttggt cttttaatga acgggagtca atacgagatt tatgaccata
     2281 tttttttaat cggtcatttg ctattatttc ccagttaact cgccaggctt caatgttttc
     2341 tctattattc cattctattt gtttgttccc aaatccattt gggctaacag ttcttaaagt
     2401 tagcattaca tgagcatgag ggttatgact atcaagatca tgaaatgcta tgtctgcaat
     2461 cattccataa tccacgaaat tttcttggca gtaatcgagg acgatctgct tcttctggcc
     2521 gtcgtccagc tccttgggga tggctatgtc cagataacgg cataattcgg catcctttcg
     2581 cttctcgcca gcctctacgg cattccagag ggtgcctgag tccttcacca gttcggcagg
     2641 tgtgccggca ggggagagga tagcagagcc gaaaaatccc tctttttttg aataatccca
     2701 ggttgccccg gttcgattat cttttgtctt ttctcggttt tgatatgcag ataaataaac
     2761 cgaagattta ccttctgatc ttcgtaattt tttaaagctg atatgaaatg tcgccatagt
     2821 ctacccctta ctttttttgt cttttgaatt ttggttttta gccccgcagg gcccatacgt
     2881 cgcaagcgac gtgtaagtgg gcattactaa tattattttt tcggttgcac ctcaaaataa
     2941 taagtaatga accccaggct tcgcccaata ttaaatatta tttgttttat tctaaaatat
     3001 atttgactaa agtaaataat ggaggtaata aataacaaaa taaataatta ggaggttatt
     3061 atgaataaac ttataacaga ggcagaaggt gcaaaaatcg aaagtgtttt taaatacata
     3121 ttagaggcat ctgaaagcat attaatagag catgggcatg ttattacggg gcaaataata
     3181 aaaaacaaaa agttctttgc tgataacggg cataaagatt ttgaaaggtt agagtattac
     3241 cttagctcgg caattagttc tcttgtcgag gataaggtaa cagaaggcat taaagacatt
     3301 aacacggatg agaaccttgt ttttaaaaaa ataaatgaat tgagtgacaa ggaaaaatta
     3361 attttagcag agtgcattat taaacatttt gaatattcaa atgttagagg aatgcaaaag
     3421 gcatttaata tttttggggg gtaatggatg agtaaaacga cagcacagca gatcgcagat
     3481 ttgcaaaata aactacaagc attaagaaca aaaaaagcaa gacaggacag aagggaagaa
     3541 acaagacaaa aaataatcct gggtgcagaa gtggctaagg ttttaggtaa aaaaccggag
     3601 gaaattaaca aggctttaat tcttgggttg ttaagtaaaa taaatgaact tgacgaagaa
     3661 gaaaaacaag aattcacacg attaggatta aatattttag aagagtggaa gaatgaaaat
     3721 cgtaaaccgt gaacaataaa aaaccagccg atcttggctg gttttttact gtgaagggtt
     3781 gccaggcttg cctggaggtc ttaaaaaggc tcagagaggc tctcagggct tatgggtggg
     3841 tttgttgatt gttgggtacc tgagtgcctc tcagttcgag atacagggcc cggatacggg
     3901 gaaaggggaa aaaatccccc caggggtgga gggtttggtt aggttagggg gaaaaactgc
     3961 ttagaagtcg ttacaggctg ttttgaggca tatctaag
//
