LOCUS       NZ_CP072415            10275 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B32278 plasmid p2B32278, complete
            sequence.
ACCESSION   NZ_CP072415
VERSION     NZ_CP072415.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18478623
            Assembly: GCF_017742935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Complete genome sequencing of K. pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler Hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,413
            CDSs (total)                      :: 5,285
            Genes (coding)                    :: 5,141
            CDSs (with protein)               :: 5,141
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 63 of 144
            Pseudo Genes (incomplete)         :: 82 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 20 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B32278"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2B32278"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2014"
                     /collected_by="Christian Medical College"
     gene            28..141
                     /locus_tag="J6256_RS27335"
     CDS             28..141
                     /locus_tag="J6256_RS27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(510..>1471)
                     /locus_tag="J6256_RS26745"
                     /old_locus_tag="J6256_26740"
                     /pseudo=""
     CDS             complement(510..>1471)
                     /locus_tag="J6256_RS26745"
                     /old_locus_tag="J6256_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            1665..3206
                     /gene="istA"
                     /locus_tag="J6256_RS26750"
                     /old_locus_tag="J6256_26745"
     CDS             1665..3206
                     /gene="istA"
                     /locus_tag="J6256_RS26750"
                     /old_locus_tag="J6256_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="WP_002431311.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYANR
                     AAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLWEE
                     YCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPETGEI
                     RTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSATSRA
                     CKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSL
                     ASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKKVRVHAD
                     YHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYSTRNEHMPE
                     AHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNLHKKYSKAR
                     LNAACARALETKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRGSEYYH"
                     /name="istA"
     gene            3221..3967
                     /gene="istB"
                     /locus_tag="J6256_RS26755"
                     /old_locus_tag="J6256_26750"
     CDS             3221..3967
                     /gene="istB"
                     /locus_tag="J6256_RS26755"
                     /old_locus_tag="J6256_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="WP_001016257.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTAE
                     ELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNILLT
                     GATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNSDLL
                     LLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAILDRL
                     VHGSIKIELKGESMRKIQSPLTEGDQ"
                     /name="istB"
     gene            complement(<4052..4216)
                     /locus_tag="J6256_RS26760"
                     /old_locus_tag="J6256_26755"
                     /pseudo=""
     CDS             complement(<4052..4216)
                     /locus_tag="J6256_RS26760"
                     /old_locus_tag="J6256_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(4405..5397)
                     /locus_tag="J6256_RS26765"
                     /old_locus_tag="J6256_26760"
     CDS             complement(4405..5397)
                     /locus_tag="J6256_RS26765"
                     /old_locus_tag="J6256_26760"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            complement(5484..6181)
                     /locus_tag="J6256_RS26770"
                     /old_locus_tag="J6256_26765"
     CDS             complement(join(5484..5932,5932..6181))
                     /locus_tag="J6256_RS26770"
                     /old_locus_tag="J6256_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            6465..6575
                     /locus_tag="J6256_RS26775"
                     /old_locus_tag="J6256_26770"
     CDS             6465..6575
                     /locus_tag="J6256_RS26775"
                     /old_locus_tag="J6256_26770"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_139102090.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRR"
                     /name="small membrane protein"
     gene            7629..7850
                     /locus_tag="J6256_RS26780"
                     /old_locus_tag="J6256_26775"
     CDS             7629..7850
                     /locus_tag="J6256_RS26780"
                     /old_locus_tag="J6256_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            7843..8730
                     /locus_tag="J6256_RS26785"
                     /old_locus_tag="J6256_26780"
     CDS             7843..8730
                     /locus_tag="J6256_RS26785"
                     /old_locus_tag="J6256_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_071579754.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKF
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPHQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            8727..9425
                     /locus_tag="J6256_RS26790"
                     /old_locus_tag="J6256_26785"
     CDS             8727..9425
                     /locus_tag="J6256_RS26790"
                     /old_locus_tag="J6256_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579755.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEAKQELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(9432..9830)
                     /locus_tag="J6256_RS26795"
                     /old_locus_tag="J6256_26790"
     CDS             complement(9432..9830)
                     /locus_tag="J6256_RS26795"
                     /old_locus_tag="J6256_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071579756.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRFFGAFAPVSVEQEEEQVSGQRDTVTSDHNEQAIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9830..10021)
                     /locus_tag="J6256_RS26800"
                     /old_locus_tag="J6256_26795"
     CDS             complement(9830..10021)
                     /locus_tag="J6256_RS26800"
                     /old_locus_tag="J6256_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_071579757.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAGADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          29..141
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="100.0"
                     /evalue="5.74e-46"
ORIGIN
        1 aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
       61 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacatgcaaa agcgccactg
      121 gcagcagcca ctggtaactg atttagagga gatagtcttg aagttatgcg ccggttaagg
      181 ctaaactaaa cgaacagttt tggtgactgt gctcctccaa gccagttacc tcggttcaaa
      241 gagttggtag ctcagagaac cttcgaaaaa accgccctgc aaggcggttt tttcgtttac
      301 tgagcaagag attacgatgc taaagaaagg atctcaagag gatcctgttc catatgccta
      361 cccctgaagt gaaaccagca ccactccgcg acaccaacca tcaaaataaa actatttaac
      421 tcgttgtttt tgataagaaa aaaacatata taatcatgat ccataatcat ccaacgcggt
      481 tgacctgccc ccaggattag atacaacctt cagttagtaa tgtcggttcg tttttcttca
      541 tatttcccgt ttcgccagcc cgccgcaaat tcagccggag tctggtagtt cagcgatgaa
      601 tgtggacgac actcgctcta atcctgcggc cagtcattaa tcgttttcct cgcgtgaacg
      661 atatcgcgga accagtgctc attgaggcac tcatccctga atcgtccgtt aaaactttca
      721 ataaatccgt tctgtgttgg cctgcccggc tggataagcc gcagctccac cccatgctca
      781 taagcccact ggtcgagtgc tctgcaggta aactccgccc cctggtcagt tcttatcgtc
      841 gccggatagc cgcgaaacag tgcaatgctg tccagcattc gcgtgacctg aacgcctgaa
      901 atcccaaagg cggcggtaat tgtcagacac tccttcgtga agtcgtccac gcaggtcagg
      961 cacttgatcc tgcgaccggt ggccagcgcg tccatgacaa aatccatcgg ccaggtcagg
     1021 ttgggcgcct ccgggcggag cagcggcaga cgttctgttg ccagaccttt acgacgtcgt
     1081 ctgcgtttta cacccagccc gttaagatgg taaagacggt acacgcgctt gtgattaaca
     1141 tgaaggcctt cacggcgcag taactgccag atgcgtcggt agccaaaacg cctgcgctcc
     1201 agtgccagct cagtgatgtg ccctgataaa tgcgcatcag ccgcgggacg ctgagcctca
     1261 tagcggcagg tcgacaggga caaacctgta agcctgcagg cacgacgttg cgacagaccg
     1321 gtcgcatcac acataaactc aacggcttcc cgtttctggt ctgtcgtcag tactttcgcc
     1381 ccagagccac ctgaagtgcc tccttatcca gcatggcttc ggcaagcagt ttcttgagtc
     1441 tggcattctc ttcctcaagc gacttaaggc gtgcgtattt tcatgaaagg agatcactca
     1501 ataacttcca tcgagatcgg gtaataacat ttgaacagat cgctgaataa catcgatgga
     1561 gatcactttt gactcatttt gttattcagt gatctccatc aatgttattg gaacttcaca
     1621 ggtgtgttga tctgtatctt ttgccattcc ggtaaaggat acctatgcca acagttccaa
     1681 tttctatgag aaaacttaaa gaaattctta ggcttaaata cggtgttgga ctcagccatc
     1741 gacaaattgg tcgtagtctt gcaatctccc cttccgttgt atccagatat gctaatcggg
     1801 cggctcaact tggcataaag cagtggccct tacctacagg atgggatgat acaaaactaa
     1861 aacatgcgtt ccttcagacc caggttaaga tgaagaagca ctctctgcct gactgggcta
     1921 cagtacaccg ggaactgcgt aataaatgcg tgacgctgca gctactctgg gaagaatact
     1981 gtgagcgtaa tccaggcggt ttttacagct ataaccatta ctgccggatg taccgtgaat
     2041 ggctcaaaac cacttcacca tcaatgcgtc aggtacataa agctggcgaa aaacttttcg
     2101 ttgattactg tggacctacc gttggcgtta ccgaccctga gaccggagaa ataagaactg
     2161 ctcaggtcat cgtagctgtt ctcggggcat caagttacac atgggcagag gccacctggt
     2221 ctcagcagct tgaagactgg gtgatgagtc atgttcgctg cttccagtgg ttgggtggcg
     2281 ttcctgaact tgttgttccg gacaatctga aaagcgccac atccagggca tgtaagtatg
     2341 atcctgacgt taaccctacc taccagcaga tgcttgagca ttataatgtc gcagttttgc
     2401 ctgcgcggcc acgtaaaccg aaagataaag ccaaagctga agttggcgtt caggttgttg
     2461 aacgctggat catggcccga atcaggcatg agatcttcta cagccttgca tcgcttaatc
     2521 agcgcattcg ggagttgctg gaaagactga ataacaaaat aatgcagaag ttgggttatt
     2581 cacgtgcaga actcttcatc cagcttgata aacccgcact gaagcctctt cctgaagcca
     2641 gttacagtta caccctggtg aagaaagtca gagttcatgc cgattaccac gtggaaatcg
     2701 acaaacatta ctactcggtt ccatgttcgc tgttaggcca gcaactggaa gcatggatct
     2761 ccggagaact ggtaagactc ttcaatcagg ggcaggaggt tgctgtgcac ccgcgcaagc
     2821 gtacttatgg ctacagtacc cgcaacgagc acatgcctga agctcatcga cagcatgcca
     2881 cctggacgcc agagcgtctt ctggaatggg cggggcacat aggcagtgaa actcatagtt
     2941 atgtgcttca tatactgaac tctcgtccac atccggaaca aagctatcgc ttctgccttg
     3001 gactcctgaa ccttcataaa aaatacagta aagccagact taatgcagca tgtgcaagag
     3061 ctctggaaac aaaggtatgg cgtctgtcag gtattaaatc gatcctggaa aaaggtctgg
     3121 ataaacaacc tgttcaggat ccaaaaccag atctgttatc cacgatggaa cacgaaaacg
     3181 tacgcggcag tgagtattac cactgatacg ggatccaatg atgaatcatc tttacgaaca
     3241 actgaccgca cttaaactca ccggcttccg tgatgcgctt aaaaagcaac ttgctcaacc
     3301 gggcacatac caggagctgg gcttcgaaga acgcctgtca ttactgacag cagaagaact
     3361 aacctgccgt gaaaacagga aggcagagcg tctgatcaaa catgcacggt tcagacttaa
     3421 tgctgagtta tcaaagctgg attatcgtaa caatagaggg ctggacaggg ccctcatccg
     3481 ttcactcagt cagggaaact ggttaaccct gaaacaaaat attttactga ccggggccac
     3541 cggcagcggt aaaacgttcc tggcatgtgc acttggtcat aatgcctgcc gacagggata
     3601 caaggtctac tattatcgcc ttaaagcgct gatggaacag tgctatcagg ggcatgctga
     3661 tggaagatac agcaaacttt tgaccaggct gaataatagc gatctgctgc ttctggatga
     3721 ctgggggctg gaacctctct catcagaaca gcgtagcgac ctgctggaaa tagtggatct
     3781 gatgtaccaa cgaggctcaa tcatcgtagt gagccagttg ccggtggaaa actggtacaa
     3841 aatgatcgga gactccacac atgcggatgc catcctagat cgactggttc atggcagtat
     3901 caagatcgaa cttaaaggag aatcaatgcg gaaaatacaa tctccgttga ccgaaggaga
     3961 tcagtgaagg taatttaaaa acggttctgt gaaagtgaca cgaaccgatc tccatcgatg
     4021 ttactcaccg atctccttca cggtaatacg caaggcgctt aacctcgggc acctccatac
     4081 cgccatactt cttacgccag gtgtaaaaga tggcgtcgga aatggcgtgc ttacggcaga
     4141 gctcacgggc agaaaccccg gcttcggcct cgcggagaat actgatgatc tgttcgtcgg
     4201 aaaaacgctt cttcatgagg atgtcctcat gtggcttatg aagacattac taacatcacg
     4261 gtgtattaac caacggggag caggtcactc cgtgtatgcc ccggtttttt cgcgctgtcg
     4321 ctgtctaaac tggctgtaaa aatttacgcc tactgggatg ggctcgcagt aggttcatta
     4381 accaattatt aaatgctcat gttatcaaac atggttcttt ttttttatta ttatcttcct
     4441 ttctattgtg taatatgcaa cgcagcctat tactatcgaa gtaacaaaac atagaaactt
     4501 ttcaggtgcg ttgttatgag ttagtttaga aaatgcaaac acagcgtaaa ggtgtgttag
     4561 ataaatggag tatgaagctg ttcctatata aatcaatgct ttctttagag gtattttatc
     4621 tacacttacc tttgatgtta tgtatatgac tacgcatgct ggaatcccag atgttagaaa
     4681 tgtaatgagt tggtagttat tatatggctc aggtaagtga atgtggccta taatggtgca
     4741 actcgtgatt attattgcta ttgatagcaa tatagggaat gcattttttt tgtatgtgta
     4801 cccattgtac gccaatccaa ttaaggagcc aagtatgaag tctaatatta gatagttacc
     4861 aatcatgtga tgaatagaat tatctttcca gcccgctaat ccgccgttaa ctccacccgc
     4921 taaaaaaaat aaaacaagca tggtgaatga agtggataag ataacattcc ttgccccttt
     4981 gaatatgtac atgcacaatg aaaatactat gtaaaaatat aattcatacg ttaatgtcca
     5041 gcaaacaaag tttatcctat catagttacc atgaatagtt gggaggaggg ttatgttatg
     5101 aaaaatatca gatgcactta tgtttttata gaaatatgaa aaaataataa gaggcagtga
     5161 taacacaagg taaggtggat atattcttaa tattctgctt tttataaatt gataccctgt
     5221 tctgtttttg cggtatgtaa tagtcattaa ataaccgctt agcacaaaaa acagatcaac
     5281 accaaagata cccgaagagc caattacagg tacgcttgcg ccataaatgt gtgtaagaac
     5341 tactgaacag gctgctatgg ctcgaataac ttgtagtgac tcgatacttt tattcatgaa
     5401 tttacatact gaaatgggtt attgaggtat tttactggaa aaaactggat gtatcactaa
     5461 ttacttgtgg tgatgacttc aacttactga tagtgtttta tgttcagata atgcccgatg
     5521 actttgtcat gcagctccac cgattttgag aacgacagtg acttcctgcc cagccttgcc
     5581 agatactgcc tcagattcag gttatgtcgc tcaatgcgct gcgtatatcg cttgctgata
     5641 acgtgcagtt ctcccttcag gcgtgattca tacagcggcc agccatccgt catccatacc
     5701 acgacctcaa aggccgacag caggctcaga agacgctcca gtgtggccag agtgcgttca
     5761 ccgaacacgt gcgccacaac cgtcctccgt atcctgtcat acgcgtaaaa caaccagcgc
     5821 tggcgtgatt tagcgccgac gtaaccccac tgttcgtcca tttccgcgca aacaatgacg
     5881 tcactgcccg gttgtatgcg tgagtttacc gactgcggcc tgagtttttt aagtgtcgta
     5941 aaatcgtgtt gaggccaacg cccataatgc gtgcactggc gcgacatccg acgccattca
     6001 tggccatatc aataattttc tggtgtgtac cgggctgaga ggcggtgtaa gtgaactgta
     6061 gctgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggca gtacttttac
     6121 cgttacgcac cacgccttca gtagctgagc aggaaggaca actgatggag atggaagcca
     6181 cgggagcacc tcaaaaacac catcgtacac taaatcagta agttggcacc attaccgtga
     6241 tttgttatat acccaccacg ggggcattag tttttttatt tttttgatga tatgtacaaa
     6301 atcagtggtt aggaataatc ttagcagcct taacaaacac atacaaaaac agcggttatg
     6361 tttcatctca ccacaataag taacgacagt atcatgctaa aaaaagatga cattatcttt
     6421 atgaaatcat aacaggctgt ttttattaaa ttaatcaggc atttatggac tggatttcac
     6481 ttgctatcgc cattgctcta cttgttgtag ctgtctactc aggaatctca tacttcagag
     6541 aaaaaaaaac aatcagccgt gtatttaggc gatagcgccc tatttaccat aatcccttct
     6601 tacgcgcacc gcgaaaacgg cctcagggcg tttctggtgg ttaacgggca aaaggtggtg
     6661 gcaagtacac cgaaaaccgc cagaaacgcg cctgatttat tttacgcata tgcatggcta
     6721 tgcacaccac cgcatagccg gtgatttcgg ccgagaaaag ggttctggac gttaaaagca
     6781 taaaagaacg ggccatccag gcccgtcaaa tgaggacaac tctgtaaaag gaccgtcgca
     6841 cctggtgcgg tgccggcgac aagaaaaaga tagctcatat cagcccctca ccggagaaaa
     6901 atatttcctg gccacctcaa ccgccacacg ctgtgttccc gttaactcgc gcagataacg
     6961 caggaacatc ctgttttcct ctgcatgcgc ctcctcactg ccctgtgcgt ggtattccac
     7021 tatcgcgttc accacggact caccgacctg catcgtctga cctcatctgc actttcatca
     7081 caccgtttca gatcccgcct cctcgccatg atgtgaaacg gtgaccgggc aggcaaattt
     7141 tttgtctgtc cggtcaccgt tggcggggtc cggggcggtc agtcccggtg ttttttattc
     7201 cgccaaatca gcacctgcga cacccgctat gtacgacgca taaaatgcgc ctgtacatag
     7261 cgggtgtcgc tattaaactg agtcaggcgc aacatttaaa aaacaacatt cacacgcacg
     7321 aaaaacctgg ctagtattta gtcaaaccat gaggaggcgc actctgacgc gcacctgcct
     7381 accggcagca ggtaaaacag ccgggtcccc ccggggatac tgtaccggac agcccaatcc
     7441 gagaatcctg agaaaacagc caaaatctgc gagaccggca atcaggtcag gccattccct
     7501 ggcttaaggg acgtttttac agccgcgagc agtccgggct taaccacgtt acggtttaaa
     7561 cgagggcgat cggcgcaggc cgcagtggat ttccactggc gttcttacaa ctcaacaccg
     7621 gagttcctgt ggaatttgat accgccgccg aactggccgc cctgcaggcg cagacccgcc
     7681 gtatccgcca ggtccgctac cgtccctccc gccttgaccg ctataccggc gaactgctga
     7741 gcctgtacca ggccggggcc agtgcagcgg agctgcaacg ctggctccgg gcccggagaa
     7801 tcaaagtcgt gttgtccacg gttacccgct ggctggaaaa aaatggctaa attcgccagc
     7861 gctgcccgcc aggcggctgg cgtgataaaa ccactccagg gaaatattct gcgctctgtc
     7921 ggtacggtcc ggaattacga gcagggactg acccgcgtcg cagagtggat gaaatttaac
     7981 cgcatcaacc acggtgattt acgcacactg acaccggcgc aggccatcac ctacctggag
     8041 cagcgcgggg agcaggtcgg ccagaaaacg ctcgatatgg aacgccaggc catccaggcc
     8101 atgatgatcc acgtgacgca gaccctccct gccggcgaac acctgccggt ggtgaaatcg
     8161 gagcaggcac agatcctgag cagccgggcc tacagcccgg accaggtggc cctgattgcc
     8221 gcagcacaac agcccgccaa cgccctggcc accgcgatcg ccctggccgc cggattacgc
     8281 gcccatgaac tgctgaccct cgcgccccac caggagcgtg caccggatcc ccgtccggca
     8341 gcggacagta aatttgccgg tcgggagggt gtgatttaca ccgttcaggg caaaggcggc
     8401 ctggtcaggg aagttctgct gccgtcacgc ctggctgaac gtctcgagca tgtcaggctg
     8461 gccagcccgg tccgggtgac cgatcgcggc gtgttttacc agagtcagta cgccattggc
     8521 ggaggccagc gctggtcaaa cgccttcagt gccgcctcca tgcgtaccct gggctggtca
     8581 cggggggcac acggcgtgcg tcacagctac gcccagcaac ggatgcagga gctgcaaaaa
     8641 ctcggtctgg ttcgtgatat ggccctgcgt accgtgagcc aggaaatggg ccattttcgt
     8701 ccggaaatca cggagacata cctgcgatga caagaacgtt tattttattg tgtaccggtg
     8761 ttttcgccat cgcgtacgcc ctgtatgagc tgaaacatat gggcgtttac tatccggatg
     8821 ccagcaatcc gcagctgttc actggctgga aggcggtcac cgaactcggt cccgcaatcc
     8881 tggctacggg gatcgcagcc acagcgttct cgctgtggct gttcattttg tttttcagag
     8941 gctcagagtc ccgccggata cagagcgaga cagacaggta taagcaggct ctgagggagg
     9001 cagaagaggc tcacagagcc agacaaaata cccttattca gaatcacaac ctggagacag
     9061 cggccctgcg aagccactac agtggtgctg aggatgtact gcgggaagcg tttgcacaaa
     9121 aagaaagcct cttgcttcag gcggtgcaga atgcccggaa agaagccaag caggagctgg
     9181 aagaacagag ggaggcactg aatcagcgcg aatccatcct gcagaaaaag gagctggcgc
     9241 tggaaacaac cctggagaag gcccggaaat atgtggccgg ggagcggcat aacaccgtgc
     9301 tggcccgtct ggaagcagag aaagcagagg aaagacggag aaatgcctgc gcgacggcgg
     9361 aacggcgcag acggaaactg gaaaaactcc agaccactga cggaacagca gggcaaaaac
     9421 actgaggcat ttcaggactg ccagaaacgc caccaggggc ggttcgtgtt ctgccgcttc
     9481 tcttcccggt tctccagcaa caacatcgcc tgtttcaggc tgtcgatatg tgaatctttt
     9541 gaggcaagaa gggtgttcag gtgttcaatt tcctgtttca gaatgtaaat ggcctgttca
     9601 ttatggtcag aagtgactgt gtcacgctgt ccactgacct gttcttcctc ctgttcaacg
     9661 gatacgggcg caaaggcacc aaaaaaccgc aaaagctctg aagtatcgag cattttcgca
     9721 ttccgttcac cccgacaggt ggacagttca ccggctttaa ttaacctgtg cagggttgtc
     9781 ctgctcttac ccgtcaggcg tgctgcctca ctgatcgaca cgttcgccat cagtcaccct
     9841 cctgtagtgc atccagtcgg agggacagtg tacggaaaag atgctccgca tcatcgtgga
     9901 gtttctcaca aaggtcagcc cccgcatcga gatcaagctc gttaagcttc tccagcagga
     9961 gcagggactg attctggatg aatttagcca tgttcagggc agttttctgt tgtttcgtca
    10021 taaggggcct cagggcgtca gtgctcagtg taaaaccggt ctcttttccg cttcctcgct
    10081 cactcagtcg ctacgctcct gccgttcggc tgcggcgagc ggacgggaca cacagaagag
    10141 tcatcgctat caccaggagg agaagagcct gtggaaaagg ccacggcgtt gccgtttttc
    10201 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gcggtggcga
    10261 aacccgacag gactt
//
