LOCUS       NZ_CP072410            10405 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B4650 plasmid p2B4650, complete
            sequence.
ACCESSION   NZ_CP072410
VERSION     NZ_CP072410.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18478563
            Assembly: GCF_017742915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Veeraraghavan,B., Varghese,G. and Vijayakumar,S.
  TITLE     Complete genome sequencing of K. pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Veeraraghavan,B., Varghese,G. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler Hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54.93x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:56:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,408
            CDSs (total)                      :: 5,280
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 62 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 20 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072410.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B4650"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2B4650"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2015"
                     /collected_by="Christian Medical College"
     gene            255..446
                     /locus_tag="J6316_RS26805"
                     /old_locus_tag="J6316_26800"
     CDS             255..446
                     /locus_tag="J6316_RS26805"
                     /old_locus_tag="J6316_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_071579757.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAGADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            446..844
                     /locus_tag="J6316_RS26810"
                     /old_locus_tag="J6316_26805"
     CDS             446..844
                     /locus_tag="J6316_RS26810"
                     /old_locus_tag="J6316_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071579756.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRFFGAFAPVSVEQEEEQVSGQRDTVTSDHNEQAIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(851..1549)
                     /locus_tag="J6316_RS26815"
                     /old_locus_tag="J6316_26810"
     CDS             complement(851..1549)
                     /locus_tag="J6316_RS26815"
                     /old_locus_tag="J6316_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579755.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEAKQELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(1546..2433)
                     /locus_tag="J6316_RS26820"
                     /old_locus_tag="J6316_26815"
     CDS             complement(1546..2433)
                     /locus_tag="J6316_RS26820"
                     /old_locus_tag="J6316_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_071579754.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKF
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPHQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            complement(2426..2647)
                     /locus_tag="J6316_RS26825"
                     /old_locus_tag="J6316_26820"
     CDS             complement(2426..2647)
                     /locus_tag="J6316_RS26825"
                     /old_locus_tag="J6316_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            4095..4792
                     /locus_tag="J6316_RS26835"
                     /old_locus_tag="J6316_26830"
     CDS             join(4095..4344,4344..4792)
                     /locus_tag="J6316_RS26835"
                     /old_locus_tag="J6316_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            4879..5871
                     /locus_tag="J6316_RS26840"
                     /old_locus_tag="J6316_26835"
     CDS             4879..5871
                     /locus_tag="J6316_RS26840"
                     /old_locus_tag="J6316_26835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            6060..>6224
                     /locus_tag="J6316_RS26845"
                     /old_locus_tag="J6316_26840"
                     /pseudo=""
     CDS             6060..>6224
                     /locus_tag="J6316_RS26845"
                     /old_locus_tag="J6316_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(6309..7055)
                     /gene="istB"
                     /locus_tag="J6316_RS26850"
                     /old_locus_tag="J6316_26845"
     CDS             complement(6309..7055)
                     /gene="istB"
                     /locus_tag="J6316_RS26850"
                     /old_locus_tag="J6316_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="WP_001016257.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTAE
                     ELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNILLT
                     GATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNSDLL
                     LLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAILDRL
                     VHGSIKIELKGESMRKIQSPLTEGDQ"
                     /name="istB"
     gene            complement(7070..8611)
                     /gene="istA"
                     /locus_tag="J6316_RS26855"
                     /old_locus_tag="J6316_26850"
     CDS             complement(7070..8611)
                     /gene="istA"
                     /locus_tag="J6316_RS26855"
                     /old_locus_tag="J6316_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="WP_002431311.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYANR
                     AAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLWEE
                     YCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPETGEI
                     RTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSATSRA
                     CKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSL
                     ASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKKVRVHAD
                     YHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYSTRNEHMPE
                     AHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNLHKKYSKAR
                     LNAACARALETKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRGSEYYH"
                     /name="istA"
     gene            <8805..9766
                     /locus_tag="J6316_RS26860"
                     /old_locus_tag="J6316_26855"
                     /pseudo=""
     CDS             <8805..9766
                     /locus_tag="J6316_RS26860"
                     /old_locus_tag="J6316_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     Typing          complement(3..207)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.818"
                     /coverage="92.0"
                     /evalue="1.18e-39"
     Typing          complement(10136..10405)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.155"
                     /coverage="86.0"
                     /evalue="6.27e-131"
ORIGIN
        1 aagtcctgtc gggtttcgcc accgctgatt tgagcgtcag atttcgtgat gcttgtcagg
       61 ggggcggagc ctatggaaaa acggcaacgc cgtggccttt tccacaggct cttctcctcc
      121 tggtgatagc gatgactctt ctgtgtgtcc cgtccgctcg ccgcagccga acggcaggag
      181 cgtagcgact gagtgagcga ggaagcggaa aagagaccgg ttttacactg agcactgacg
      241 ccctgaggcc ccttatgacg aaacaacaga aaactgccct gaacatggct aaattcatcc
      301 agaatcagtc cctgctcctg ctggagaagc ttaacgagct tgatctcgat gcgggggctg
      361 acctttgtga gaaactccac gatgatgcgg agcatctttt ccgtacactg tccctccgac
      421 tggatgcact acaggagggt gactgatggc gaacgtgtcg atcagtgagg cagcacgcct
      481 gacgggtaag agcaggacaa ccctgcacag gttaattaaa gccggtgaac tgtccacctg
      541 tcggggtgaa cggaatgcga aaatgctcga tacttcagag cttttgcggt tttttggtgc
      601 ctttgcgccc gtatccgttg aacaggagga agaacaggtc agtggacagc gtgacacagt
      661 cacttctgac cataatgaac aggccattta cattctgaaa caggaaattg aacacctgaa
      721 cacccttctt gcctcaaaag attcacatat cgacagcctg aaacaggcga tgttgttgct
      781 ggagaaccgg gaagagaagc ggcagaacac gaaccgcccc tggtggcgtt tctggcagtc
      841 ctgaaatgcc tcagtgtttt tgccctgctg ttccgtcagt ggtctggagt ttttccagtt
      901 tccgtctgcg ccgttccgcc gtcgcgcagg catttctccg tctttcctct gctttctctg
      961 cttccagacg ggccagcacg gtgttatgcc gctccccggc cacatatttc cgggccttct
     1021 ccagggttgt ttccagcgcc agctcctttt tctgcaggat ggattcgcgc tgattcagtg
     1081 cctccctctg ttcttccagc tcctgcttgg cttctttccg ggcattctgc accgcctgaa
     1141 gcaagaggct ttctttttgt gcaaacgctt cccgcagtac atcctcagca ccactgtagt
     1201 ggcttcgcag ggccgctgtc tccaggttgt gattctgaat aagggtattt tgtctggctc
     1261 tgtgagcctc ttctgcctcc ctcagagcct gcttatacct gtctgtctcg ctctgtatcc
     1321 ggcgggactc tgagcctctg aaaaacaaaa tgaacagcca cagcgagaac gctgtggctg
     1381 cgatccccgt agccaggatt gcgggaccga gttcggtgac cgccttccag ccagtgaaca
     1441 gctgcggatt gctggcatcc ggatagtaaa cgcccatatg tttcagctca tacagggcgt
     1501 acgcgatggc gaaaacaccg gtacacaata aaataaacgt tcttgtcatc gcaggtatgt
     1561 ctccgtgatt tccggacgaa aatggcccat ttcctggctc acggtacgca gggccatatc
     1621 acgaaccaga ccgagttttt gcagctcctg catccgttgc tgggcgtagc tgtgacgcac
     1681 gccgtgtgcc ccccgtgacc agcccagggt acgcatggag gcggcactga aggcgtttga
     1741 ccagcgctgg cctccgccaa tggcgtactg actctggtaa aacacgccgc gatcggtcac
     1801 ccggaccggg ctggccagcc tgacatgctc gagacgttca gccaggcgtg acggcagcag
     1861 aacttccctg accaggccgc ctttgccctg aacggtgtaa atcacaccct cccgaccggc
     1921 aaatttactg tccgctgccg gacggggatc cggtgcacgc tcctggtggg gcgcgagggt
     1981 cagcagttca tgggcgcgta atccggcggc cagggcgatc gcggtggcca gggcgttggc
     2041 gggctgttgt gctgcggcaa tcagggccac ctggtccggg ctgtaggccc ggctgctcag
     2101 gatctgtgcc tgctccgatt tcaccaccgg caggtgttcg ccggcaggga gggtctgcgt
     2161 cacgtggatc atcatggcct ggatggcctg gcgttccata tcgagcgttt tctggccgac
     2221 ctgctccccg cgctgctcca ggtaggtgat ggcctgcgcc ggtgtcagtg tgcgtaaatc
     2281 accgtggttg atgcggttaa atttcatcca ctctgcgacg cgggtcagtc cctgctcgta
     2341 attccggacc gtaccgacag agcgcagaat atttccctgg agtggtttta tcacgccagc
     2401 cgcctggcgg gcagcgctgg cgaatttagc catttttttc cagccagcgg gtaaccgtgg
     2461 acaacacgac tttgattctc cgggcccgga gccagcgttg cagctccgct gcactggccc
     2521 cggcctggta caggctcagc agttcgccgg tatagcggtc aaggcgggag ggacggtagc
     2581 ggacctggcg gatacggcgg gtctgcgcct gcagggcggc cagttcggcg gcggtatcaa
     2641 attccacagg aactccggtg ttgagttgta agaacgccag tggaaatcca ctgcggcctg
     2701 cgccgatcgc cctcgtttaa accgtaacgt ggttaagccc ggactgctcg cggctgtaaa
     2761 aacgtccctt aagccaggga atggcctgac ctgattgccg gtctcgcaga ttttggctgt
     2821 tttctcagga ttctcggatt gggctgtccg gtacagtatc cccgggggga cccggctgtt
     2881 ttacctgctg ccggtaggca ggtgcgcgtc agagtgcgcc tcctcatggt ttgactaaat
     2941 actagccagg tttttcgtgc gtgtgaatgt tgttttttaa atgttgcgcc tgactcagtt
     3001 taatagcgac acccgctatg tacaggcgca ttttatgcgt cgtacatagc gggtgtcgca
     3061 ggtgctgatt tggcggaata aaaaacaccg ggactgaccg ccccggaccc cgccaacggt
     3121 gaccggacag acaaaaaatt tgcctgcccg gtcaccgttt cacatcatgg cgaggaggcg
     3181 ggatctgaaa cggtgtgatg aaagtgcaga tgaggtcaga cgatgcaggt cggtgagtcc
     3241 gtggtgaacg cgatagtgga ataccacgca cagggcagtg aggaggcgca tgcagaggaa
     3301 aacaggatgt tcctgcgtta tctgcgcgag ttaacgggaa cacagcgtgt ggcggttgag
     3361 gtggccagga aatatttttc tccggtgagg ggctgatatg agctatcttt ttcttgtcgc
     3421 cggcaccgca ccaggtgcga cggtcctttt acagagttgt cctcatttga cgggcctgga
     3481 tggcccgttc ttttatgctt ttaacgtcca gaaccctttt ctcggccgaa atcaccggct
     3541 atgcggtggt gtgcatagcc atgcatatgc gtaaaataaa tcaggcgcgt ttctggcggt
     3601 tttcggtgta cttgccacca ccttttgccc gttaaccacc agaaacgccc tgaggccgtt
     3661 ttcgcggtgc gcgtaagaag ggattatggt aaatagggcg ctatcgccta aatacacggc
     3721 tgattgtttt tttttctctg aagtatgaga ttcctgagta gacagctaca acaagtagag
     3781 caatggcgat agcaagtgaa atccagtcca taaatgcctg attaatttaa taaaaacagc
     3841 ctgttatgat ttcataaaga taatgtcatc tttttttagc atgatactgt cgttacttat
     3901 tgtggtgaga tgaaacataa ccgctgtttt tgtatgtgtt tgttaaggct gctaagatta
     3961 ttcctaacca ctgattttgt acatatcatc aaaaaaataa aaaaactaat gcccccgtgg
     4021 tgggtatata acaaatcacg gtaatggtgc caacttactg atttagtgta cgatggtgtt
     4081 tttgaggtgc tcccgtggct tccatctcca tcagttgtcc ttcctgctca gctactgaag
     4141 gcgtggtgcg taacggtaaa agtactgccg gacatcagcg ctatctctgc tctcactgcc
     4201 gtaaaacatg gcagctacag ttcacttaca ccgcctctca gcccggtaca caccagaaaa
     4261 ttattgatat ggccatgaat ggcgtcggat gtcgcgccag tgcacgcatt atgggcgttg
     4321 gcctcaacac gattttacga cacttaaaaa actcaggccg cagtcggtaa actcacgcat
     4381 acaaccgggc agtgacgtca ttgtttgcgc ggaaatggac gaacagtggg gttacgtcgg
     4441 cgctaaatca cgccagcgct ggttgtttta cgcgtatgac aggatacgga ggacggttgt
     4501 ggcgcacgtg ttcggtgaac gcactctggc cacactggag cgtcttctga gcctgctgtc
     4561 ggcctttgag gtcgtggtat ggatgacgga tggctggccg ctgtatgaat cacgcctgaa
     4621 gggagaactg cacgttatca gcaagcgata tacgcagcgc attgagcgac ataacctgaa
     4681 tctgaggcag tatctggcaa ggctgggcag gaagtcactg tcgttctcaa aatcggtgga
     4741 gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcagt aagttgaagt
     4801 catcaccaca agtaattagt gatacatcca gttttttcca gtaaaatacc tcaataaccc
     4861 atttcagtat gtaaattcat gaataaaagt atcgagtcac tacaagttat tcgagccata
     4921 gcagcctgtt cagtagttct tacacacatt tatggcgcaa gcgtacctgt aattggctct
     4981 tcgggtatct ttggtgttga tctgtttttt gtgctaagcg gttatttaat gactattaca
     5041 taccgcaaaa acagaacagg gtatcaattt ataaaaagca gaatattaag aatatatcca
     5101 ccttaccttg tgttatcact gcctcttatt attttttcat atttctataa aaacataagt
     5161 gcatctgata tttttcataa cataaccctc ctcccaacta ttcatggtaa ctatgatagg
     5221 ataaactttg tttgctggac attaacgtat gaattatatt tttacatagt attttcattg
     5281 tgcatgtaca tattcaaagg ggcaaggaat gttatcttat ccacttcatt caccatgctt
     5341 gttttatttt ttttagcggg tggagttaac ggcggattag cgggctggaa agataattct
     5401 attcatcaca tgattggtaa ctatctaata ttagacttca tacttggctc cttaattgga
     5461 ttggcgtaca atgggtacac atacaaaaaa aatgcattcc ctatattgct atcaatagca
     5521 ataataatca cgagttgcac cattataggc cacattcact tacctgagcc atataataac
     5581 taccaactca ttacatttct aacatctggg attccagcat gcgtagtcat atacataaca
     5641 tcaaaggtaa gtgtagataa aatacctcta aagaaagcat tgatttatat aggaacagct
     5701 tcatactcca tttatctaac acacctttac gctgtgtttg cattttctaa actaactcat
     5761 aacaacgcac ctgaaaagtt tctatgtttt gttacttcga tagtaatagg ctgcgttgca
     5821 tattacacaa tagaaaggaa gataataata aaaaaaaaga accatgtttg ataacatgag
     5881 catttaataa ttggttaatg aacctactgc gagcccatcc cagtaggcgt aaatttttac
     5941 agccagttta gacagcgaca gcgcgaaaaa accggggcat acacggagtg acctgctccc
     6001 cgttggttaa tacaccgtga tgttagtaat gtcttcataa gccacatgag gacatcctca
     6061 tgaagaagcg tttttccgac gaacagatca tcagtattct ccgcgaggcc gaagccgggg
     6121 tttctgcccg tgagctctgc cgtaagcacg ccatttccga cgccatcttt tacacctggc
     6181 gtaagaagta tggcggtatg gaggtgcccg aggttaagcg ccttgcgtat taccgtgaag
     6241 gagatcggtg agtaacatcg atggagatcg gttcgtgtca ctttcacaga accgttttta
     6301 aattaccttc actgatctcc ttcggtcaac ggagattgta ttttccgcat tgattctcct
     6361 ttaagttcga tcttgatact gccatgaacc agtcgatcta ggatggcatc cgcatgtgtg
     6421 gagtctccga tcattttgta ccagttttcc accggcaact ggctcactac gatgattgag
     6481 cctcgttggt acatcagatc cactatttcc agcaggtcgc tacgctgttc tgatgagaga
     6541 ggttccagcc cccagtcatc cagaagcagc agatcgctat tattcagcct ggtcaaaagt
     6601 ttgctgtatc ttccatcagc atgcccctga tagcactgtt ccatcagcgc tttaaggcga
     6661 taatagtaga ccttgtatcc ctgtcggcag gcattatgac caagtgcaca tgccaggaac
     6721 gttttaccgc tgccggtggc cccggtcagt aaaatatttt gtttcagggt taaccagttt
     6781 ccctgactga gtgaacggat gagggccctg tccagccctc tattgttacg ataatccagc
     6841 tttgataact cagcattaag tctgaaccgt gcatgtttga tcagacgctc tgccttcctg
     6901 ttttcacggc aggttagttc ttctgctgtc agtaatgaca ggcgttcttc gaagcccagc
     6961 tcctggtatg tgcccggttg agcaagttgc tttttaagcg catcacggaa gccggtgagt
     7021 ttaagtgcgg tcagttgttc gtaaagatga ttcatcattg gatcccgtat cagtggtaat
     7081 actcactgcc gcgtacgttt tcgtgttcca tcgtggataa cagatctggt tttggatcct
     7141 gaacaggttg tttatccaga cctttttcca ggatcgattt aatacctgac agacgccata
     7201 cctttgtttc cagagctctt gcacatgctg cattaagtct ggctttactg tattttttat
     7261 gaaggttcag gagtccaagg cagaagcgat agctttgttc cggatgtgga cgagagttca
     7321 gtatatgaag cacataacta tgagtttcac tgcctatgtg ccccgcccat tccagaagac
     7381 gctctggcgt ccaggtggca tgctgtcgat gagcttcagg catgtgctcg ttgcgggtac
     7441 tgtagccata agtacgcttg cgcgggtgca cagcaacctc ctgcccctga ttgaagagtc
     7501 ttaccagttc tccggagatc catgcttcca gttgctggcc taacagcgaa catggaaccg
     7561 agtagtaatg tttgtcgatt tccacgtggt aatcggcatg aactctgact ttcttcacca
     7621 gggtgtaact gtaactggct tcaggaagag gcttcagtgc gggtttatca agctggatga
     7681 agagttctgc acgtgaataa cccaacttct gcattatttt gttattcagt ctttccagca
     7741 actcccgaat gcgctgatta agcgatgcaa ggctgtagaa gatctcatgc ctgattcggg
     7801 ccatgatcca gcgttcaaca acctgaacgc caacttcagc tttggcttta tctttcggtt
     7861 tacgtggccg cgcaggcaaa actgcgacat tataatgctc aagcatctgc tggtaggtag
     7921 ggttaacgtc aggatcatac ttacatgccc tggatgtggc gcttttcaga ttgtccggaa
     7981 caacaagttc aggaacgcca cccaaccact ggaagcagcg aacatgactc atcacccagt
     8041 cttcaagctg ctgagaccag gtggcctctg cccatgtgta acttgatgcc ccgagaacag
     8101 ctacgatgac ctgagcagtt cttatttctc cggtctcagg gtcggtaacg ccaacggtag
     8161 gtccacagta atcaacgaaa agtttttcgc cagctttatg tacctgacgc attgatggtg
     8221 aagtggtttt gagccattca cggtacatcc ggcagtaatg gttatagctg taaaaaccgc
     8281 ctggattacg ctcacagtat tcttcccaga gtagctgcag cgtcacgcat ttattacgca
     8341 gttcccggtg tactgtagcc cagtcaggca gagagtgctt cttcatctta acctgggtct
     8401 gaaggaacgc atgttttagt tttgtatcat cccatcctgt aggtaagggc cactgcttta
     8461 tgccaagttg agccgcccga ttagcatatc tggatacaac ggaaggggag attgcaagac
     8521 tacgaccaat ttgtcgatgg ctgagtccaa caccgtattt aagcctaaga atttctttaa
     8581 gttttctcat agaaattgga actgttggca taggtatcct ttaccggaat ggcaaaagat
     8641 acagatcaac acacctgtga agttccaata acattgatgg agatcactga ataacaaaat
     8701 gagtcaaaag tgatctccat cgatgttatt cagcgatctg ttcaaatgtt attacccgat
     8761 ctcgatggaa gttattgagt gatctccttt catgaaaata cgcacgcctt aagtcgcttg
     8821 aggaagagaa tgccagactc aagaaactgc ttgccgaagc catgctggat aaggaggcac
     8881 ttcaggtggc tctggggcga aagtactgac gacagaccag aaacgggaag ccgttgagtt
     8941 tatgtgtgat gcgaccggtc tgtcgcaacg tcgtgcctgc aggcttacag gtttgtccct
     9001 gtcgacctgc cgctatgagg ctcagcgtcc cgcggctgat gcgcatttat cagggcacat
     9061 cactgagctg gcactggagc gcaggcgttt tggctaccga cgcatctggc agttactgcg
     9121 ccgtgaaggc cttcatgtta atcacaagcg cgtgtaccgt ctttaccatc ttaacgggct
     9181 gggtgtaaaa cgcagacgac gtcgtaaagg tctggcaaca gaacgtctgc cgctgctccg
     9241 cccggaggcg cccaacctga cctggccgat ggattttgtc atggacgcgc tggccaccgg
     9301 tcgcaggatc aagtgcctga cctgcgtgga cgacttcacg aaggagtgtc tgacaattac
     9361 cgccgccttt gggatttcag gcgttcaggt cacgcgaatg ctggacagca ttgcactgtt
     9421 tcgcggctat ccggcgacga taagaactga ccagggggcg gagtttacct gcagagcact
     9481 cgaccagtgg gcttatgagc atggggtgga gctgcggctt atccagccgg gcaggccaac
     9541 acagaacgga tttattgaaa gttttaacgg acgattcagg gatgagtgcc tcaatgagca
     9601 ctggttccgc gatatcgttc acgcgaggaa aacgattaat gactggccgc aggattagag
     9661 cgagtgtcgt ccacattcat cgctgaacta ccagactccg gctgaatttg cggcgggctg
     9721 gcgaaacggg aaatatgaag aaaaacgaac cgacattact aactgaaggt tgtatctaat
     9781 cctgggggca ggtcaaccgc gttggatgat tatggatcat gattatatat gtttttttct
     9841 tatcaaaaac aacgagttaa atagttttat tttgatggtt ggtgtcgcgg agtggtgctg
     9901 gtttcacttc aggggtaggc atatggaaca ggatcctctt gagatccttt ctttagcatc
     9961 gtaatctctt gctcagtaaa cgaaaaaacc gccttgcagg gcggtttttt cgaaggttct
    10021 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcacagt caccaaaact
    10081 gttcgtttag tttagcctta accggcgcat aacttcaaga ctatctcctc taaatcagtt
    10141 accagtggct gctgccagtg gcgcttttgc atgtcttacc gggttggact caagacgata
    10201 gttaccggat aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt
    10261 ggagcgaact gccttcccgg aactgagtgt caggcgtgga atgagataaa cgcggccata
    10321 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac
    10381 cagggggaaa cgcctggtat cttta
//
