LOCUS       NZ_CP072304             5679 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain KSK20 plasmid p4KSK20, complete
            sequence.
ACCESSION   NZ_CP072304
VERSION     NZ_CP072304.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18452712
            Assembly: GCF_017726555.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Yadav,B., Makwana,N., Kharat,A.S., Veeraraghavan,B., Vijayakumar,S.
            and Priya,M.
  TITLE     Complete genome sequencing of Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Yadav,B., Makwana,N., Kharat,A.S., Veeraraghavan,B., Vijayakumar,S.
            and Priya,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler Hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.41x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:30:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,254
            CDSs (total)                      :: 4,158
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 21 of 83
            Pseudo Genes (incomplete)         :: 62 of 83
            Pseudo Genes (internal stop)      :: 13 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072304.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="KSK20"
                     /isolation_source="Respiratory specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p4KSK20"
                     /geo_loc_name="India: New Delhi"
                     /lat_lon="28.5402 N 77.1662 E"
                     /collection_date="2020"
                     /collected_by="Jawaharlal Nehru University"
     gene            complement(join(5627..5679,1..445))
                     /locus_tag="J6I82_RS21135"
                     /old_locus_tag="J6I82_21135"
     CDS             complement(join(5627..5679,1..445))
                     /locus_tag="J6I82_RS21135"
                     /old_locus_tag="J6I82_21135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031380830.1"
                     /translation="MATAIRADYCTNMTINNCSFSGFNNCIEMNFCTNFNILGSGFFNS
                     NNAVFLSNCYGKGAISNNYTHNVNNLISVADSKLKYPLPSKNKEKHKIIKKEQKKNSSI
                     RNIEFNKKIKELINLEANQKFIKRNHQLACKIKYIKANLGKLEAESLLFLTQALMQYDL
                     QS"
                     /name="hypothetical protein"
     gene            complement(606..1022)
                     /locus_tag="J6I82_RS21140"
                     /old_locus_tag="J6I82_21140"
     CDS             complement(606..1022)
                     /locus_tag="J6I82_RS21140"
                     /old_locus_tag="J6I82_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_025464995.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     ILLKTAEIDPNDMKWQIWKELGQETLNHRKKDKK"
                     /name="conjugal transfer protein TraD"
     gene            1303..1458
                     /locus_tag="J6I82_RS21145"
                     /old_locus_tag="J6I82_21145"
     CDS             1303..1458
                     /locus_tag="J6I82_RS21145"
                     /old_locus_tag="J6I82_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="WP_161772599.1"
                     /translation="MQLKQVLGKIKDISFGVIAAPLIIIIMMWASILDQRTFKKELDRK
                     KAQEKT"
                     /name="heme exporter protein CcmD"
     gene            1458..2381
                     /locus_tag="J6I82_RS21150"
                     /old_locus_tag="J6I82_21150"
     CDS             1458..2381
                     /locus_tag="J6I82_RS21150"
                     /old_locus_tag="J6I82_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002011644.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQSDLEHSDYGN
                     MPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGSKY
                     PYQFAIHNPKAMDGNDQPHIHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDNTG
                     KSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKDPEKKLLPSQAKD
                     PEIREALQQSRTAYKELERLDLGDPKNDLKDLKNSPISDKEIKQGIESFKADFASFKQL
                     ALQQYKEQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            2378..2878
                     /locus_tag="J6I82_RS21155"
                     /old_locus_tag="J6I82_21155"
     CDS             2378..2878
                     /locus_tag="J6I82_RS21155"
                     /old_locus_tag="J6I82_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025464991.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQKVIAEQTH
                     STVSKSLNTGLQEGTAELIKTAKALGHLKNSIESTSDQFSWKIITIILGIFAILMLGII
                     WLFSVYIKPLENIPQIESLRQQGIQFDIGSCTVGEESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     gene            2919..3215
                     /locus_tag="J6I82_RS21160"
                     /old_locus_tag="J6I82_21160"
     CDS             2919..3215
                     /locus_tag="J6I82_RS21160"
                     /old_locus_tag="J6I82_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002011632.1"
                     /translation="MPLIEYYGLLFEWHDQKMDLVYRGREITFEEVCSIFLDPALISFE
                     DVGHYDEQRLISVGLSNRGRLLTVVWVERGDVARIITAFEPSHHQKRRYSNAK"
                     /name="BrnT family toxin"
     gene            3205..3420
                     /locus_tag="J6I82_RS21165"
                     /old_locus_tag="J6I82_21165"
     CDS             3205..3420
                     /locus_tag="J6I82_RS21165"
                     /old_locus_tag="J6I82_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002011639.1"
                     /translation="MQNELERYKNFDPTNAPLVKPSFVKKLQERHTIAQAQAFDADVVA
                     WLSSQDNETKQHINDMVRHIMALKRA"
                     /name="hypothetical protein"
     gene            4356..5135
                     /locus_tag="J6I82_RS21170"
                     /old_locus_tag="J6I82_21170"
     CDS             4356..5135
                     /locus_tag="J6I82_RS21170"
                     /old_locus_tag="J6I82_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_002011642.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEALNLLNSVAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELTETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     ARG             4357..5132
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.23"
                     /coverage="100.0"
     ARG             4357..5132
                     /name="aph(3')-VI"
                     /gene="aph(3')-VI"
                     /gene_name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.23"
                     /coverage="100.0"
     Typing          1459..2378
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagcttctaa ttttccaaga tttgctttta tatattttat tttacaagct aactgatgat
       61 ttctcttaat aaatttttgg ttagcttcta agttaataag ttctttaatt tttttattaa
      121 attctatatt ccttattgaa ctatttttct tctgctcttt ttttatgatt ttatgctttt
      181 ccttattttt tgaaggaaga gggtatttta gttttgaatc agcaactgaa attaaattat
      241 ttacattatg agtatagttg tttgatatag ctccttttcc gtagcaatta gataaaaaga
      301 cagcattatt agaattaaaa aaaccactac ctaatatatt gaagttagta caaaagttca
      361 tctctataca attgttaaac cctgaaaaag agcaattgtt tatagtcata tttgtacaat
      421 aatctgcacg aattgcagtc gccataaaat tcatcctacc ttagttgcct tattattaag
      481 ataatacacc ttatgcaaag taatctttaa ttatctttat aaatcaaaaa tataattttt
      541 agaccctctg tatatagatg agttttttac gtgcttagat aaagttgaat aaaaattctg
      601 aacaatcact ttttgtcttt tttacgatga tttaaagttt cttgtcctag ctctttccaa
      661 atttgccatt tcatatcatt aggatcaatt tcagcagttt ttagaagaat gccaagtaat
      721 tcggctgggt cacgctgatc taggttagct atttcaaata gtgcgcctaa ttgaatgaga
      781 tgacgtgtac gagctttacg ttctgcttcc ttttcggcat tcaataattt tctaactgca
      841 actttttgct tatttgctcg atctatggct ttttcagctt tgatcaaggc attgattttt
      901 ttctcttggt cgggtgttct gtactgaatt ttggctaatt caagtaaaag tttttgagca
      961 tcactaggat tctttaaagc agaaatatat gtgatttttt cagacagcca atcattattc
     1021 atattcaatt ccattaattc atataccgca aaagcacagg ctcaatactc aaagaacgat
     1081 aacaaaatat ttacgttcga gtaacgtaat tttgttaaag tcgttctgga gattatcgcc
     1141 tgtacttcca tcaaaaattt gatggcgcgc ttagacgttt ctcaatttca ttgagaaacc
     1201 taagcacgtt agggacttca tccctaaaac cctgaaactt ctttttttcg ttcaattgaa
     1261 aattgaatga aaaaaagaag taccaaagct aggagcaaca gcatgcaact aaaacaggta
     1321 ttgggaaaaa tcaaagatat tagttttggc gtgatcgcag caccgctaat tatcatcatt
     1381 atgatgtggg cttcgatact cgatcaaaga acatttaaga aagaactaga ccgaaaaaag
     1441 gcacaggaaa aaacctaatg gcgatagctc gtttaagtgt gaaagttggt aaggcaggta
     1501 aggcagcacc gcatgccgaa tatattgatc gggacgaaga aaaaaagctg aaacaggaac
     1561 aatccgacct tgaacatagt gactatggaa atatgccgaa atgggctgaa cataatccga
     1621 ttaccttttg gcaagcagcc gacctttacg agcgtaaaaa tggcagtacc tatcgagaat
     1681 atgaaattgc cctgcctaga gagatgaatg ctgagcaacg cctagaactg gttgaagact
     1741 ttattcagtc tgaaattggc tcaaaatatc cgtatcagtt tgcaattcat aaccctaaag
     1801 caatggacgg caacgatcag ccccacatcc acctcatgtt taatgaacgt ctgcaagatg
     1861 gaatcgaacg agatccagag caatatttca aacgctataa cagcaagaat cctgaacgtg
     1921 gcggagctaa aaaagacaat acaggcaaga gttaccagga acgaaaaact gatattaaag
     1981 acctacgcca aaggtgggcg gatttatgca atagccattt ggaaaaacac caaattgaca
     2041 gccgtattga tatgcgaagc tacaaagagc aaggcataga gaaagaccct gaaaagaaac
     2101 tcttaccaag ccaagccaaa gaccctgaaa tcagggaagc cttgcaacag tcacgtacag
     2161 cctataaaga gcttgagcga ctagatttag gcgaccctaa aaacgacctg aaagacctca
     2221 aaaacagccc tatctcagac aaagaaatta aacagggcat tgagagtttt aaggctgatt
     2281 ttgccagctt taaacagctt gccttacagc agtacaaaga acagcagaaa ttagaacgag
     2341 agcaacagaa aacaatgaag tttagaggga tgagccgatg acacctgatg aaaaaaagtt
     2401 atatgcactg atggcagttg cagaacaaca gcagaaccta gtcaatcagg ccgttaaaga
     2461 gcttcaaatc acgaaagaac acattcagaa ggtcatcgca gagcaaaccc atagcactgt
     2521 atcaaaatcg ctaaatacgg gcttacagga gggtacagca gagcttatca agaccgctaa
     2581 ggcacttgga catttaaaaa actcgattga atcaactagt gatcaatttt catggaaaat
     2641 catcaccatt attttaggca ttttcgctat tctgatgctt ggcatcattt ggttattcag
     2701 tgtttatatc aaacctttag aaaatatccc tcagatcgag agcttaagac agcaaggtat
     2761 ccagtttgat attggaagct gtactgttgg agaggaatct aaaccgtgtg ttcgtgtgat
     2821 gaaaaagcag tgcggatatg gaaaaaatgg agatttttgt gtcatagacc caaaataata
     2881 ggcatatttt ttatttcacg agaggaaaag aggtaatcat gccattaatt gaatattacg
     2941 gcttgttatt tgaatggcat gaccaaaaaa tggatttagt ctatcgaggg cgagaaatca
     3001 cgttcgagga ggtctgtagt attttccttg atcctgcatt gatctctttt gaggatgtag
     3061 gacactatga tgaacaacga ctaatatcag tcggtttaag caatcgtggt agactattaa
     3121 cagtggtttg ggttgaacgt ggagatgttg cgagaataat cactgcattt gaaccatcac
     3181 atcaccaaaa gcggaggtac agcaatgcaa aatgaactag aacgctataa aaattttgac
     3241 cctaccaatg cgccattggt taaacctagc tttgtgaaga aattacagga acgccatacc
     3301 atagctcaag cacaggcttt tgatgctgat gtagttgcat ggttaagttc acaagataat
     3361 gaaacaaaac aacatattaa cgatatggtt cgccatatta tggcattgaa acgtgcttga
     3421 ttagggattt agtcatgatt agatatgacc tctctttttt gtcttaatat ctgattgatc
     3481 aaagaaaaag cccattgaat cttgcaactc aatgggcttt cgctttatat tcagcggtac
     3541 gcagttattt gatggtggaa catcaagtaa cgaagatcag cagagctacc aactcgtgca
     3601 gatttcacac aaaggcttgt acctgaactc tattttcaca ataaacagtg tttagatcaa
     3661 gactcaaaat gataagtctt cgttaatccg tgttttaaat catcaaataa gcatcctaga
     3721 tcgagctttg cctgctcctg cgtgtccgtt ttacggattg gcatgcgttg catgtggagg
     3781 gaataagtgt cactggtgca acgtagcgat agtcgaaagg ctaaaccgct acaggggaat
     3841 ctcacaaaag cggagcgtta agagtagttg tataggttgg ttaagttgta tgttggggaa
     3901 accctcgcag agataccgcc atacttcgac agggagcgtg tggggagagg tgcaagacct
     3961 ttagtccttg tctgcatagt ttgcaaagct atgtgcggat acgaccgata tacgtggctc
     4021 cgaagccaaa agaatatgcg gtaaatcggc aatacttaaa ggatgcgtca tttttttaaa
     4081 tgaccgcctt tagggagagt attgccgaaa ctctctcaac cgtttccaaa gccaaagcac
     4141 gaagtgcgac gctttgggta cgagttgaga agttgagagc attttttttt aggaagtata
     4201 gtagtgcgga taatcaaata ctcctttttc tgcttaggct attgaaccag ttttgaattc
     4261 gccccatatt tttgctacag tgaaccaaat taagatcatc tatttactag gccttgcatt
     4321 tgcggggttt taatgctgaa taaaaggaaa acttgatgga attgcccaat attattcaac
     4381 aatttattgg aaacagtgtt ttagagccaa ataaaattgg tcagtcgcca tcggatgttt
     4441 attcttttaa tcgaaataat gaaacttttt ttcttaagcg atctagcact ttatatacag
     4501 agaccacata cagtgtctct cgcgaagcga aaatgttgag ttggctctct gagaaattaa
     4561 aggtgcctga actcatcatg acttttcagg atgagcagtt tgaattcatg atcactaaag
     4621 cgatcaatgc aaaaccaatt tcagcgcttt ttttaacaga ccaagaattg cttgctatct
     4681 ataaggaggc actcaatctg ttaaattcag ttgctattat tgattgtcca tttatttcaa
     4741 acattgatca tcggttaaaa gagtcaaaat tttttattga taaccaactc cttgacgata
     4801 tagatcaaga tgattttgac gctgaattat ggggagacca taaaacttac ctaagtctat
     4861 ggaatgagtt aactgagact cgtgttgaag aaagattggt tttttctcat ggcgatatca
     4921 cggatagtaa tatttttata gataaattca atgaaattta ttttttagat cttggccgtg
     4981 ctgggttagc agatgaattt gtagatatat cctttgttga acgttgccta agagaggatg
     5041 catcggagga aactgctaaa atatttttaa agcatttaaa aaatgataga cctgacaaaa
     5101 ggaattattt tttaaaactt gatgaattga attgattcca agcattatct aaaaatactt
     5161 aattgtcttt ttacgtcgct aaattttaaa taaataagtg aagagcgtta gtggagctat
     5221 tgatttaaag ttttatatca ccaataaatc taaattttgg tttttaaaat gagcttattt
     5281 aaaatagcta aaactgttta ttagcttaac ttgtgattta agtttttttg aatataaaag
     5341 tgttaattta ttagctttgc aagtttattt aaatttcatc gtgcaacatt tttaaccaat
     5401 tttattatcg atggtatttt tttgtctgct tttcttttct tagtctttta tctatctgtc
     5461 agccatatag tattttatta aattcttatg ggaaatgacg aatgttaaat tatcttaaga
     5521 gctttaataa tatcaatact tggcagagta aaacttgaag tgcgacataa accacctaat
     5581 taatttaaag ggtttatgga gtatataaaa ttgtcatacc atcatcttaa ctttgaagat
     5641 cgtactgcat taatgcttga gtcaagaaaa gaaggcttt
//
