LOCUS       NZ_CP072284             5679 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain KSK10 plasmid p4KSK10, complete
            sequence.
ACCESSION   NZ_CP072284
VERSION     NZ_CP072284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18452342
            Assembly: GCF_017724155.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Yadav,B., Makwana,N., Kharat,A.S., Veeraraghavan,B., Vijayakumar,S.
            and Priya,M.
  TITLE     Complete genome sequencing of Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Yadav,B., Makwana,N., Kharat,A.S., Veeraraghavan,B., Vijayakumar,S.
            and Priya,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler Hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 167.89x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:12:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,277
            CDSs (total)                      :: 4,181
            Genes (coding)                    :: 4,098
            CDSs (with protein)               :: 4,098
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 19 of 83
            Pseudo Genes (incomplete)         :: 63 of 83
            Pseudo Genes (internal stop)      :: 13 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072284.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="KSK10"
                     /isolation_source="Respiratory specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p4KSK10"
                     /geo_loc_name="India: New Delhi"
                     /lat_lon="28.5402 N 77.1662 E"
                     /collection_date="2020"
                     /collected_by="Jawaharlal Nehru University"
     gene            complement(join(5068..5679,1..168))
                     /locus_tag="J6F37_RS21250"
                     /old_locus_tag="J6F37_21250"
     CDS             complement(join(5068..5679,1..168))
                     /locus_tag="J6F37_RS21250"
                     /old_locus_tag="J6F37_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_002011642.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEALNLLNSVAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELTETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(1104..1319)
                     /locus_tag="J6F37_RS21255"
                     /old_locus_tag="J6F37_21255"
     CDS             complement(1104..1319)
                     /locus_tag="J6F37_RS21255"
                     /old_locus_tag="J6F37_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002011639.1"
                     /translation="MQNELERYKNFDPTNAPLVKPSFVKKLQERHTIAQAQAFDADVVA
                     WLSSQDNETKQHINDMVRHIMALKRA"
                     /name="hypothetical protein"
     gene            complement(1309..1605)
                     /locus_tag="J6F37_RS21260"
                     /old_locus_tag="J6F37_21260"
     CDS             complement(1309..1605)
                     /locus_tag="J6F37_RS21260"
                     /old_locus_tag="J6F37_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002011632.1"
                     /translation="MPLIEYYGLLFEWHDQKMDLVYRGREITFEEVCSIFLDPALISFE
                     DVGHYDEQRLISVGLSNRGRLLTVVWVERGDVARIITAFEPSHHQKRRYSNAK"
                     /name="BrnT family toxin"
     gene            complement(1646..2146)
                     /locus_tag="J6F37_RS21265"
                     /old_locus_tag="J6F37_21265"
     CDS             complement(1646..2146)
                     /locus_tag="J6F37_RS21265"
                     /old_locus_tag="J6F37_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025464991.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQKVIAEQTH
                     STVSKSLNTGLQEGTAELIKTAKALGHLKNSIESTSDQFSWKIITIILGIFAILMLGII
                     WLFSVYIKPLENIPQIESLRQQGIQFDIGSCTVGEESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     gene            complement(2143..3066)
                     /locus_tag="J6F37_RS21270"
                     /old_locus_tag="J6F37_21270"
     CDS             complement(2143..3066)
                     /locus_tag="J6F37_RS21270"
                     /old_locus_tag="J6F37_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002011644.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQSDLEHSDYGN
                     MPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGSKY
                     PYQFAIHNPKAMDGNDQPHIHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDNTG
                     KSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKDPEKKLLPSQAKD
                     PEIREALQQSRTAYKELERLDLGDPKNDLKDLKNSPISDKEIKQGIESFKADFASFKQL
                     ALQQYKEQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            complement(3066..3221)
                     /locus_tag="J6F37_RS21275"
                     /old_locus_tag="J6F37_21275"
     CDS             complement(3066..3221)
                     /locus_tag="J6F37_RS21275"
                     /old_locus_tag="J6F37_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="WP_161772599.1"
                     /translation="MQLKQVLGKIKDISFGVIAAPLIIIIMMWASILDQRTFKKELDRK
                     KAQEKT"
                     /name="heme exporter protein CcmD"
     gene            3502..3918
                     /locus_tag="J6F37_RS21280"
                     /old_locus_tag="J6F37_21280"
     CDS             3502..3918
                     /locus_tag="J6F37_RS21280"
                     /old_locus_tag="J6F37_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_025464995.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     ILLKTAEIDPNDMKWQIWKELGQETLNHRKKDKK"
                     /name="conjugal transfer protein TraD"
     gene            4079..4576
                     /locus_tag="J6F37_RS21285"
                     /old_locus_tag="J6F37_21285"
     CDS             4079..4576
                     /locus_tag="J6F37_RS21285"
                     /old_locus_tag="J6F37_21285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031380830.1"
                     /translation="MATAIRADYCTNMTINNCSFSGFNNCIEMNFCTNFNILGSGFFNS
                     NNAVFLSNCYGKGAISNNYTHNVNNLISVADSKLKYPLPSKNKEKHKIIKKEQKKNSSI
                     RNIEFNKKIKELINLEANQKFIKRNHQLACKIKYIKANLGKLEAESLLFLTQALMQYDL
                     QS"
                     /name="hypothetical protein"
     ARG             complement(5069..5679)
                     /name="APH(3')-VIa"
                     /gene="APH(3')-VIa"
                     /gene_name="APH(3')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.01"
                     /coverage="78.38"
     ARG             complement(2..168)
                     /name="APH(3')-VIb"
                     /gene="APH(3')-VIb"
                     /gene_name="APH(3')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="21.62"
     Typing          complement(2147..3066)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgagagaca ctgtatgtgg tctctgtata taaagtgcta gatcgcttaa gaaaaaaagt
       61 ttcattattt cgattaaaag aataaacatc cgatggcgac tgaccaattt tatttggctc
      121 taaaacactg tttccaataa attgttgaat aatattgggc aattccatca agttttcctt
      181 ttattcagca ttaaaacccc gcaaatgcaa ggcctagtaa atagatgatc ttaatttggt
      241 tcactgtagc aaaaatatgg ggcgaattca aaactggttc aatagcctaa gcagaaaaag
      301 gagtatttga ttatccgcac tactatactt cctaaaaaaa aatgctctca acttctcaac
      361 tcgtacccaa agcgtcgcac ttcgtgcttt ggctttggaa acggttgaga gagtttcggc
      421 aatactctcc ctaaaggcgg tcatttaaaa aaatgacgca tcctttaagt attgccgatt
      481 taccgcatat tcttttggct tcggagccac gtatatcggt cgtatccgca catagctttg
      541 caaactatgc agacaaggac taaaggtctt gcacctctcc ccacacgctc cctgtcgaag
      601 tatggcggta tctctgcgag ggtttcccca acatacaact taaccaacct atacaactac
      661 tcttaacgct ccgcttttgt gagattcccc tgtagcggtt tagcctttcg actatcgcta
      721 cgttgcacca gtgacactta ttccctccac atgcaacgca tgccaatccg taaaacggac
      781 acgcaggagc aggcaaagct cgatctagga tgcttatttg atgatttaaa acacggatta
      841 acgaagactt atcattttga gtcttgatct aaacactgtt tattgtgaaa atagagttca
      901 ggtacaagcc tttgtgtgaa atctgcacga gttggtagct ctgctgatct tcgttacttg
      961 atgttccacc atcaaataac tgcgtaccgc tgaatataaa gcgaaagccc attgagttgc
     1021 aagattcaat gggctttttc tttgatcaat cagatattaa gacaaaaaag agaggtcata
     1081 tctaatcatg actaaatccc taatcaagca cgtttcaatg ccataatatg gcgaaccata
     1141 tcgttaatat gttgttttgt ttcattatct tgtgaactta accatgcaac tacatcagca
     1201 tcaaaagcct gtgcttgagc tatggtatgg cgttcctgta atttcttcac aaagctaggt
     1261 ttaaccaatg gcgcattggt agggtcaaaa tttttatagc gttctagttc attttgcatt
     1321 gctgtacctc cgcttttggt gatgtgatgg ttcaaatgca gtgattattc tcgcaacatc
     1381 tccacgttca acccaaacca ctgttaatag tctaccacga ttgcttaaac cgactgatat
     1441 tagtcgttgt tcatcatagt gtcctacatc ctcaaaagag atcaatgcag gatcaaggaa
     1501 aatactacag acctcctcga acgtgatttc tcgccctcga tagactaaat ccattttttg
     1561 gtcatgccat tcaaataaca agccgtaata ttcaattaat ggcatgatta cctcttttcc
     1621 tctcgtgaaa taaaaaatat gcctattatt ttgggtctat gacacaaaaa tctccatttt
     1681 ttccatatcc gcactgcttt ttcatcacac gaacacacgg tttagattcc tctccaacag
     1741 tacagcttcc aatatcaaac tggatacctt gctgtcttaa gctctcgatc tgagggatat
     1801 tttctaaagg tttgatataa acactgaata accaaatgat gccaagcatc agaatagcga
     1861 aaatgcctaa aataatggtg atgattttcc atgaaaattg atcactagtt gattcaatcg
     1921 agttttttaa atgtccaagt gccttagcgg tcttgataag ctctgctgta ccctcctgta
     1981 agcccgtatt tagcgatttt gatacagtgc tatgggtttg ctctgcgatg accttctgaa
     2041 tgtgttcttt cgtgatttga agctctttaa cggcctgatt gactaggttc tgctgttgtt
     2101 ctgcaactgc catcagtgca tataactttt tttcatcagg tgtcatcggc tcatccctct
     2161 aaacttcatt gttttctgtt gctctcgttc taatttctgc tgttctttgt actgctgtaa
     2221 ggcaagctgt ttaaagctgg caaaatcagc cttaaaactc tcaatgccct gtttaatttc
     2281 tttgtctgag atagggctgt ttttgaggtc tttcaggtcg tttttagggt cgcctaaatc
     2341 tagtcgctca agctctttat aggctgtacg tgactgttgc aaggcttccc tgatttcagg
     2401 gtctttggct tggcttggta agagtttctt ttcagggtct ttctctatgc cttgctcttt
     2461 gtagcttcgc atatcaatac ggctgtcaat ttggtgtttt tccaaatggc tattgcataa
     2521 atccgcccac ctttggcgta ggtctttaat atcagttttt cgttcctggt aactcttgcc
     2581 tgtattgtct tttttagctc cgccacgttc aggattcttg ctgttatagc gtttgaaata
     2641 ttgctctgga tctcgttcga ttccatcttg cagacgttca ttaaacatga ggtggatgtg
     2701 gggctgatcg ttgccgtcca ttgctttagg gttatgaatt gcaaactgat acggatattt
     2761 tgagccaatt tcagactgaa taaagtcttc aaccagttct aggcgttgct cagcattcat
     2821 ctctctaggc agggcaattt catattctcg ataggtactg ccatttttac gctcgtaaag
     2881 gtcggctgct tgccaaaagg taatcggatt atgttcagcc catttcggca tatttccata
     2941 gtcactatgt tcaaggtcgg attgttcctg tttcagcttt ttttcttcgt cccgatcaat
     3001 atattcggca tgcggtgctg ccttacctgc cttaccaact ttcacactta aacgagctat
     3061 cgccattagg ttttttcctg tgcctttttt cggtctagtt ctttcttaaa tgttctttga
     3121 tcgagtatcg aagcccacat cataatgatg ataattagcg gtgctgcgat cacgccaaaa
     3181 ctaatatctt tgatttttcc caatacctgt tttagttgca tgctgttgct cctagctttg
     3241 gtacttcttt ttttcattca attttcaatt gaacgaaaaa aagaagtttc agggttttag
     3301 ggatgaagtc cctaacgtgc ttaggtttct caatgaaatt gagaaacgtc taagcgcgcc
     3361 atcaaatttt tgatggaagt acaggcgata atctccagaa cgactttaac aaaattacgt
     3421 tactcgaacg taaatatttt gttatcgttc tttgagtatt gagcctgtgc ttttgcggta
     3481 tatgaattaa tggaattgaa tatgaataat gattggctgt ctgaaaaaat cacatatatt
     3541 tctgctttaa agaatcctag tgatgctcaa aaacttttac ttgaattagc caaaattcag
     3601 tacagaacac ccgaccaaga gaaaaaaatc aatgccttga tcaaagctga aaaagccata
     3661 gatcgagcaa ataagcaaaa agttgcagtt agaaaattat tgaatgccga aaaggaagca
     3721 gaacgtaaag ctcgtacacg tcatctcatt caattaggcg cactatttga aatagctaac
     3781 ctagatcagc gtgacccagc cgaattactt ggcattcttc taaaaactgc tgaaattgat
     3841 cctaatgata tgaaatggca aatttggaaa gagctaggac aagaaacttt aaatcatcgt
     3901 aaaaaagaca aaaagtgatt gttcagaatt tttattcaac tttatctaag cacgtaaaaa
     3961 actcatctat atacagaggg tctaaaaatt atatttttga tttataaaga taattaaaga
     4021 ttactttgca taaggtgtat tatcttaata ataaggcaac taaggtagga tgaattttat
     4081 ggcgactgca attcgtgcag attattgtac aaatatgact ataaacaatt gctctttttc
     4141 agggtttaac aattgtatag agatgaactt ttgtactaac ttcaatatat taggtagtgg
     4201 tttttttaat tctaataatg ctgtcttttt atctaattgc tacggaaaag gagctatatc
     4261 aaacaactat actcataatg taaataattt aatttcagtt gctgattcaa aactaaaata
     4321 ccctcttcct tcaaaaaata aggaaaagca taaaatcata aaaaaagagc agaagaaaaa
     4381 tagttcaata aggaatatag aatttaataa aaaaattaaa gaacttatta acttagaagc
     4441 taaccaaaaa tttattaaga gaaatcatca gttagcttgt aaaataaaat atataaaagc
     4501 aaatcttgga aaattagaag ctgaaagcct tcttttcttg actcaagcat taatgcagta
     4561 cgatcttcaa agttaagatg atggtatgac aattttatat actccataaa ccctttaaat
     4621 taattaggtg gtttatgtcg cacttcaagt tttactctgc caagtattga tattattaaa
     4681 gctcttaaga taatttaaca ttcgtcattt cccataagaa tttaataaaa tactatatgg
     4741 ctgacagata gataaaagac taagaaaaga aaagcagaca aaaaaatacc atcgataata
     4801 aaattggtta aaaatgttgc acgatgaaat ttaaataaac ttgcaaagct aataaattaa
     4861 cacttttata ttcaaaaaaa cttaaatcac aagttaagct aataaacagt tttagctatt
     4921 ttaaataagc tcattttaaa aaccaaaatt tagatttatt ggtgatataa aactttaaat
     4981 caatagctcc actaacgctc ttcacttatt tatttaaaat ttagcgacgt aaaaagacaa
     5041 ttaagtattt ttagataatg cttggaatca attcaattca tcaagtttta aaaaataatt
     5101 ccttttgtca ggtctatcat tttttaaatg ctttaaaaat attttagcag tttcctccga
     5161 tgcatcctct cttaggcaac gttcaacaaa ggatatatct acaaattcat ctgctaaccc
     5221 agcacggcca agatctaaaa aataaatttc attgaattta tctataaaaa tattactatc
     5281 cgtgatatcg ccatgagaaa aaaccaatct ttcttcaaca cgagtctcag ttaactcatt
     5341 ccatagactt aggtaagttt tatggtctcc ccataattca gcgtcaaaat catcttgatc
     5401 tatatcgtca aggagttggt tatcaataaa aaattttgac tcttttaacc gatgatcaat
     5461 gtttgaaata aatggacaat caataatagc aactgaattt aacagattga gtgcctcctt
     5521 atagatagca agcaattctt ggtctgttaa aaaaagcgct gaaattggtt ttgcattgat
     5581 cgctttagtg atcatgaatt caaactgctc atcctgaaaa gtcatgatga gttcaggcac
     5641 ctttaatttc tcagagagcc aactcaacat tttcgcttc
//
