LOCUS       NZ_CP072259             7143 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Bacteroides caccae CL03T12C61 plasmid pBcacCL03T12-1, complete
            sequence.
ACCESSION   NZ_CP072259
VERSION     NZ_CP072259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16451178
            Assembly: GCF_018292205.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides caccae CL03T12C61
  ORGANISM  Bacteroides caccae CL03T12C61
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Garcia-Bayona,L., Coyne,M.J. and Comstock,L.E.
  TITLE     Mobile Type VI secretion system loci of the gut Bacteroidales
            display extensive intra-ecosystem transfer, multi-species spread and
            geographical clustering
  JOURNAL   PLoS Genet 17 (4), e1009541 (2021)
   PUBMED   33901198
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Coyne,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) Department of Medicine/Division of
            Infectious Diseases, Brigham & Women's Hospital, 181 Longwood
            Avenue, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-OCT-2020
            Assembly Method                   :: FALCON-Unzip v. 1.2.0
            Assembly Name                     :: BcacCL03T12C61_pbv2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018292205.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 06:46:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,504
            Genes (coding)                    :: 4,387
            CDSs (with protein)               :: 4,387
            Genes (RNA)                       :: 90
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 42 of 117
            Pseudo Genes (incomplete)         :: 74 of 117
            Pseudo Genes (internal stop)      :: 31 of 117
            Pseudo Genes (multiple problems)  :: 25 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072259.1.
            Sequencing by the Institute for Genome Sciences, Genomics Resource
            Center, School of Medicine, University of Maryland, 801 W.
            Baltimore Street, Baltimore, MD 21201, USA. Gene calling method:
            Prodigal version 2.6.3. Annotated using a custom modification of
            Prokka version 1.14.6.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Bacteroides caccae CL03T12C61"
                     /mol_type="genomic DNA"
                     /strain="CL03T12C61"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:997873"
                     /plasmid="pBcacCL03T12-1"
                     /geo_loc_name="USA: Boston"
                     /lat_lon="42.3361 N 71.1075 W"
                     /collection_date="2009-05"
     gene            1..1023
                     /locus_tag="INE72_RS22805"
                     /old_locus_tag="INE72_04543"
                     /db_xref="GeneID:75115717"
     CDS             1..1023
                     /locus_tag="INE72_RS22805"
                     /old_locus_tag="INE72_04543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683039.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_005683039.1"
                     /db_xref="GeneID:75115717"
                     /translation="MARPIKTQSKDIQQSNYFTTAKYDYTLQEKIILYRVAEQAFEYRE
                     ANKEWFKEHEGEYIAKDHVSFRLPITHFMTKEQRADGSGKCYDDVVAAFKSLTSKRIEF
                     QTKDAFSFGALLNSADREPGEGIIYFTVHKFVWQSALDFTKGFTKLDLVVAMELKSAYS
                     MRFYEIARRWSDTKIWKISVEDFRDLFGCKEKYPAFNDMKRYIIDNAKEELDRVAPWSF
                     SYEQHKVGRKVESFTFYFYEIPKNKREETERKELLSKYPQAVIRPEVKDWFKNKMGFNT
                     GQIRSNVQLIDELQRIFQNGTIPELEETFQYITKQGFRPQENIGWFIQNLKKKIENAQL
                     "
                     /name="replication initiation protein"
     gene            1053..1451
                     /locus_tag="INE72_RS22810"
                     /old_locus_tag="INE72_04544"
                     /db_xref="GeneID:75115718"
     CDS             1053..1451
                     /locus_tag="INE72_RS22810"
                     /old_locus_tag="INE72_04544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005683040.1"
                     /db_xref="GeneID:75115718"
                     /translation="MKPTDYIEWDNLKNIPFFLCQVVEDKENKDIDIYYLGKRVFHDYD
                     HVGHYMRSAIILFRRIKNRTADWVNLQNLWTLRNCIRENYNHGIGVDSLIYGEDFDGEN
                     PETLTPLTKKRFDLIVKRIQELDKYATI"
                     /name="hypothetical protein"
     gene            1763..4081
                     /locus_tag="INE72_RS22815"
                     /old_locus_tag="INE72_04545"
                     /db_xref="GeneID:75115719"
     CDS             1763..4081
                     /locus_tag="INE72_RS22815"
                     /old_locus_tag="INE72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781630.1"
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="WP_224200430.1"
                     /db_xref="GeneID:75115719"
                     /translation="MDLNKKHLLYLIVFFLFGNLNAFGQSINISGRILSQGTQDAVEYA
                     NIVLFKQDSIFLQGTTSDSIGRFEFMNLSPNDYVLSVSCMGFEPKKILLQNLAETVEID
                     VFLNESALSLGEVVISASSTISKINQRIVFPTKLQLSHSANGMQLLNTMMLPGININPM
                     ANTISSSDGGKVILQINDVNATSEEILTLQPRQIRRVEYSDYAGIRYGEASKIVNYIVT
                     RDDKGGVIGVDLMNSLNILAGGDVFFAKFNKGKSEYALNYTAAFQKINSNNRTRTGSYQ
                     FENSPMLQRDEIGEGGDYSYQMHDVTLGYNYQQSDSTFFNAKLKYALTNHPHNDFRSSL
                     KEKGVDVGRIFDGVSQKINTPTVDLYYELGLKNRQKIYTNLVGSYSKVETHRNYIEYDN
                     IDTLFREQFNLVSDKYSLIAEGIYEKSFTHGSLKLGAKHIQSFTQQEVRQNGTFESNLN
                     QAESSVFTEWNHSKDNLSYSLGLRVNRVHLSNSSIDKSYYNLLPKAMFGYRLSENSFIR
                     YDVEMSQTNPTLIELTDTEIRLDPYLAEKGNLSLKPYRNLNNNLFYENKKGLFTLNVNL
                     RHHYKHNPIMESIKRQGDIFLIIPENMKSWSKYNAEMTLKLGMIKNILQFSLTGGYNHF
                     SSQGNNYSHTHSNFYYSANVLAMYKKWMFIGQIQPFEEMLYGETVIKNGNYHYLAVQYN
                     ANSFSFGIGAFNPFKNVSRSIMENKNALAPFRRESFSNASQTIVATLTWNFSFGKTHSS
                     SSKSLNNQDADYGVKSSYK"
                     /name="TonB-dependent receptor"
     gene            4090..4308
                     /locus_tag="INE72_RS22820"
                     /old_locus_tag="INE72_04546"
                     /db_xref="GeneID:75115720"
     CDS             4090..4308
                     /locus_tag="INE72_RS22820"
                     /old_locus_tag="INE72_04546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005683042.1"
                     /db_xref="GeneID:75115720"
                     /translation="MNKNSLELFSLLSENSHVTDEQMQNAYKSFVEQITTISQSQQSYP
                     EVFRMLNRTRIELDSLESSSLYELEKK"
                     /name="hypothetical protein"
     gene            complement(4310..4765)
                     /gene="mbpC"
                     /locus_tag="INE72_RS22825"
                     /old_locus_tag="INE72_04547"
                     /db_xref="GeneID:75115721"
     CDS             complement(4310..4765)
                     /gene="mbpC"
                     /locus_tag="INE72_RS22825"
                     /old_locus_tag="INE72_04547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_005683043.1"
                     /db_xref="GeneID:75115721"
                     /translation="MEKTSYQEKEVSTSNTKRGQYSPRRKDTAPKVNPQLAVELIFQEI
                     KRIEAFTKRIEDATQRKVQIDEKSLENAENSLKSVLSGFEKQSYRMKNGGYVDKRISLW
                     TILCAVFSLLFACLMCYLWVDAAKDRDNYKMYYEYYQKENNPQSRDS"
                     /name="mbpC"
     gene            complement(4722..5516)
                     /gene="mbpB"
                     /locus_tag="INE72_RS22830"
                     /old_locus_tag="INE72_04548"
                     /db_xref="GeneID:75115722"
     CDS             complement(4722..5516)
                     /gene="mbpB"
                     /locus_tag="INE72_RS22830"
                     /old_locus_tag="INE72_04548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_005683044.1"
                     /db_xref="GeneID:75115722"
                     /translation="MIAKGKSISHGTAALEYDLSKQIAGKDAATEVCRNGLYGTTGADM
                     VQEMKPYFADFPNVRNNCLRFEVSPSIEESAQYTSEDWRKLADDFLTRMGLQNHQYVVI
                     RHSGTESRKNQAHLHILANRVSMSGELYRDNWIGKRATEAANGMARERNLVQAQDIGKA
                     NRQDIKSGMDAVLQKLERFDFGSFKEEMEKAGYPIREARASTGKLNGYYVKAKSGTEYK
                     ASEIGKNYTLAHIEKTHFKLHRQTLGQSYGKDIISGKGGLHL"
                     /name="mbpB"
     gene            complement(5513..5809)
                     /gene="mbpA"
                     /locus_tag="INE72_RS22835"
                     /db_xref="GeneID:75115723"
     CDS             complement(5513..5809)
                     /gene="mbpA"
                     /locus_tag="INE72_RS22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_032838721.1"
                     /db_xref="GeneID:75115723"
                     /translation="MEVKRTEKITFRCTVLEKAALAEQALRCGLTTSEYCRSLSLGGQP
                     KERYSEEEKALLRDIAKIRGDLQRLNNYFGGRQYREVFEENRVIIEKLKKLLR"
                     /name="mbpA"
     gene            complement(6119..6460)
                     /locus_tag="INE72_RS22840"
                     /old_locus_tag="INE72_04549"
                     /db_xref="GeneID:75115724"
     CDS             complement(6119..6460)
                     /locus_tag="INE72_RS22840"
                     /old_locus_tag="INE72_04549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005683045.1"
                     /db_xref="GeneID:75115724"
                     /translation="MSFEVKTTPHFEREAKILAKRYKSFKSDLKELVEDLAKDPKIGTE
                     LSPGLRKIRLAIVSKGKGKSGGARVITFTICSSESEGRVYLVDVYDKSDYSTVSVPVLK
                     KMIAELDIL"
                     /name="hypothetical protein"
     gene            complement(6450..6683)
                     /locus_tag="INE72_RS22845"
                     /old_locus_tag="INE72_04550"
                     /db_xref="GeneID:75115725"
     CDS             complement(6450..6683)
                     /locus_tag="INE72_RS22845"
                     /old_locus_tag="INE72_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005683046.1"
                     /db_xref="GeneID:75115725"
                     /translation="MQVVSLSKFRTNQTATLLRAIQGESVFLTSRLGDFKLVPVSAEEK
                     IATRIKEGLNDVKRIESGEIPGKNANAFLDEL"
                     /name="hypothetical protein"
ORIGIN
        1 atggcaagac caataaaaac gcaatcaaag gatatacagc agagcaacta ttttacaaca
       61 gccaagtatg actatacact gcaagaaaaa attatcctct atcgtgtagc agaacaagca
      121 tttgaatata gggaagctaa caaggaatgg ttcaaagaac atgaaggaga atatattgca
      181 aaagaccatg tttctttcag gctacccata acgcacttca tgaccaaaga acaaagagct
      241 gatggaagcg gtaaatgcta tgatgatgta gtggctgcct tcaagtcttt aacaagcaaa
      301 agaatagaat ttcagacaaa agatgccttc tcctttggcg cacttctgaa ctcagccgac
      361 agagagccgg gagagggaat aatatatttt accgtacata agtttgtatg gcagtccgct
      421 ttggacttta ccaaaggatt tacaaagctg gatttggttg tagctatgga gttgaaatca
      481 gcctactcca tgcgattcta tgagatagct cgaagatgga gtgataccaa aatatggaaa
      541 atctctgtag aagatttcag agacttattt gggtgcaagg agaaatatcc agcatttaac
      601 gatatgaaac gatatattat agacaatgcc aaagaagaac tggatagggt tgctccgtgg
      661 tcattctctt atgaacagca caaagtggga agaaaggttg agagttttac cttttacttc
      721 tatgagatac ccaaaaacaa aagggaagaa acagaaagaa aagagctact atcaaagtac
      781 ccacaagcag taatcagacc agaggtaaag gattggttca aaaacaagat gggattcaat
      841 acaggacaaa tcagaagtaa tgtgcagctc attgatgaac tgcaacgtat cttccaaaac
      901 ggcacaatcc cggaactgga ggaaacattc caatacataa caaaacaagg gttcagacca
      961 caggaaaata tagggtggtt tattcagaac ctcaaaaaga agatagaaaa cgcacaactt
     1021 tgattatgtc acacaatcaa aacaatgata ttatgaaacc aactgactac atcgagtggg
     1081 ataatctgaa gaatatccct ttcttcctct gccaagtggt cgaggacaaa gaaaacaaag
     1141 acattgacat ctattattta ggaaagcgag tgtttcacga ttacgaccat gtggggcact
     1201 atatgcgctc tgccattata ctgtttcgaa gaatcaagaa ccgcacggca gactgggtaa
     1261 accttcagaa cctctggacg ctgcgcaact gtattcgtga gaattacaat cacggtatag
     1321 gtgtggatag tctgatttac ggagaggact ttgacggcga gaatcccgaa acacttactc
     1381 cattgaccaa aaaacgtttt gacctcatag taaagcgaat acaggaactt gacaaatacg
     1441 caacaattta aattgcatag acgacttcta tcaaatacaa caaaaaggag aaactataat
     1501 gttaaaaccc aattatcgag aacaatatta ctaataaatt cttatctttg catggaattt
     1561 agaacaacaa gttatgtcga acttacagag tgcattagca cgtactttta gaatacgttc
     1621 atagtctata ctgagaatcc tttctcagtt ttcttatagc cttcaattct tctgagggca
     1681 aattttctat tatcatttat taatcaagat tgtgatgcag gtattatatc tgtgtctata
     1741 tctcatttat aagaaaacaa gtatggactt aaataaaaaa catttattat atcttatcgt
     1801 attttttctg ttcggtaact tgaatgcttt tggacaatca attaacattt caggaaggat
     1861 acttagtcaa ggtacacaag atgctgttga atatgctaat attgttctat ttaaacagga
     1921 ttctatattt ttacaaggaa ctacaagtga ttctattggg agatttgaat ttatgaacct
     1981 ttctccgaat gattatgtat tgtcagtatc ttgtatgggt tttgagccta aaaagatatt
     2041 gctacaaaat cttgctgaaa cagtagaaat agatgtgttc ttgaatgaaa gtgcactgtc
     2101 ccttggtgaa gttgttattt cagcttcatc tacaataagc aaaataaatc agcgaatcgt
     2161 ttttccgaca aaattacaac ttagtcattc ggcaaacgga atgcaactat taaatacaat
     2221 gatgcttccg ggaataaaca tcaatccgat ggcaaacacg atttcatctt ccgacggtgg
     2281 aaaagtcata ttgcaaatca atgacgtaaa tgccacatcg gaagagatac tgactctgca
     2341 accgcgtcaa atcaggaggg ttgaatattc ggattatgca ggaataaggt acggagaagc
     2401 atctaaaatt gtcaattata ttgttacaag ggacgataag ggtggtgtta ttggtgtgga
     2461 cttgatgaac tccctgaata ttctggcggg tggcgatgtg tttttcgcca agtttaataa
     2521 aggcaagtcg gaatatgcac tgaactacac cgctgctttt cagaaaatca acagcaataa
     2581 tcggacacgt acaggaagct atcaatttga aaattcacct atgttgcaga gagatgaaat
     2641 cggggaaggc ggagattatt cgtatcagat gcatgatgtc actttagggt acaactatca
     2701 acaaagcgat tcgacattct tcaatgcaaa attaaagtat gctttgacaa accatccgca
     2761 taatgacttt agaagtagcc tgaaagaaaa aggtgtagat gtaggcagaa tatttgatgg
     2821 tgttagtcag aaaatcaaca cgccaacagt agatttatat tatgaacttg gtttgaaaaa
     2881 tcggcaaaaa atttatacta accttgtcgg gagttattct aaagttgaaa cacatcgtaa
     2941 ttacatagaa tacgacaata ttgatacgct tttcagggag caattcaatt tggtttccga
     3001 caaatattca ctgattgcag aaggtattta cgagaaaagt tttactcatg gtagtttgaa
     3061 acttggagca aagcacattc aatcgtttac tcaacaagaa gttcgacaaa atggaacttt
     3121 cgagtcgaat ttgaatcaag ctgaatcttc cgtctttaca gaatggaatc acagtaaaga
     3181 taatctcagc tattctctgg ggcttagagt taatcgggta cacctatcca atagctcaat
     3241 cgataagagt tattataatc ttctgccaaa agccatgttt ggatacaggc taagtgaaaa
     3301 ttcttttatt cgttacgatg tggagatgag tcaaacaaac ccgactttga ttgaattaac
     3361 cgatacggaa attagacttg acccttatct tgccgaaaaa ggaaacttgt cattgaaacc
     3421 ttatcgcaac ctgaacaaca acctttttta cgaaaacaaa aaaggcttgt ttacgctcaa
     3481 tgtaaaccta cgacatcatt ataagcacaa ccctatcatg gagtcaataa agcgacaagg
     3541 tgacatcttc cttataatac ctgaaaatat gaaaagttgg agcaagtata atgcagaaat
     3601 gaccttaaag ttgggcatga taaagaacat cttacagttt tcgcttacag gcggatacaa
     3661 tcatttcagc agtcaaggaa acaactattc gcatacacac tctaattttt attatagcgc
     3721 aaatgtgttg gcaatgtata aaaaatggat gtttatcggt cagatacagc cgtttgaaga
     3781 aatgttatat ggtgaaaccg ttattaagaa cggcaattac cactacttag ccgttcaata
     3841 caatgcaaat agtttttcgt tcggcatagg tgcattcaat ccttttaaga atgtttcccg
     3901 aagtataatg gaaaacaaga atgctctagc tccttttaga agagagagtt ttagtaatgc
     3961 ttctcagact attgtagcta ctcttacgtg gaatttcagc tttggaaaaa ctcatagctc
     4021 aagcagtaaa tctctgaata atcaggacgc tgattatggt gtgaaaagta gttataaata
     4081 gagtctgtta tgaataaaaa cagtttggaa ttattcagtt tattatctga aaattcacat
     4141 gttacagacg aacaaatgca aaatgcctat aagtcttttg tagaacagat aacaacaatc
     4201 agtcaatctc aacaaagcta tcctgaagta tttcggatgc taaatagaac tcgtattgag
     4261 cttgattctt tggaatcatc atctctttac gagttggaaa aaaaataagc taagaatctc
     4321 tgctttgggg attattttct ttttgataat attcataata catcttatag ttatccctat
     4381 cttttgctgc atccacccat aagtagcaca tcagacaagc aaacaacagg gagaaaacgg
     4441 cacaaagtat agtccaaagt gaaatacgtt tgtccacata gccaccattc ttcatccggt
     4501 agctctgctt ctcgaaaccc gacaagacac tttttagact gttttcggca ttttcaagac
     4561 ttttctcatc tatctgtact tttctctgtg ttgcgtcctc tatgcgcttt gtaaaggctt
     4621 ctatgcgctt tatctcctga aatatcagtt caaccgcaag ctgcgggttc actttcggtg
     4681 ctgtgtcctt tctgcgtggg ctgtactgcc cccttttggt gttagaggtg gagacctcct
     4741 tttcctgata tgatgtcttt tccataactc tgtcctaatg tctgtctgtg taacttgaaa
     4801 tgggttttct ctatgtgtgc gagggtgtag ttcttgccta tctcactggc tttgtactcc
     4861 gttccggact ttgccttgac atagtatccg ttcagcttcc cagtactggc tctggcttca
     4921 cggataggat atccagcttt ctccatttcc tccttgaaac tgccgaagtc gaacctctcg
     4981 agcttctgaa gaactgcgtc catgccggac ttgatgtcct gtctgtttgc cttgcctata
     5041 tcctgagcct gtacgagatt cctctccctc gccatgccgt tggctgcttc ggtcgccctc
     5101 ttgcctatcc agttgtcccg gtaaagctcg ccggacatgg aaacccggtt tgccaatata
     5161 tggaggtgtg cctggttctt ccggctctcc gttcctgaat gccttatcac cacatactgg
     5221 tggttctgca accccatgcg tgtaaggaag tcgtctgcta gcttcctcca gtcctcactg
     5281 gtgtattgtg cgctttcctc aatgctcgga cttacctcaa agcggaggca gttgttcctc
     5341 acgttgggga aatcggcaaa atacggcttc atctcctgaa ccatgtccgc acctgttgtg
     5401 ccgtaaagtc cgttccggca cacttcggtg gctgcgtcct ttccggctat ctgcttggag
     5461 aggtcgtact ccagtgctgc agtaccgtgt gaaatgcttt ttcctttggc tatcatcgca
     5521 agagcttttt aagtttttca attataaccc ggttctcctc gaacacctcc ctgtactggc
     5581 gaccgccgaa gtagttgttc agcctttgga ggtcgcccct tatcttggct atgtcacgaa
     5641 ggagagcctt ttcctcctca ctatacctct ccttcggctg accgccaagt gaaaggcttc
     5701 tacagtactc cgaagtggta agaccgcatc ggagagcctg ttccgcaagt gcagccttct
     5761 caagtaccgt acagcggaat gttatctttt cagtgcgttt tacttccatg ccggctaaga
     5821 taggcaaaaa aatccttttg agcaaagcga gaaaggcaag aaatgtccga cacggacact
     5881 tcttgctatg taaacacgct gcataggaat acacccacgc ttaccgactg cacagcccac
     5941 acactttttt ccctttttcc ccatgcttca taccaacgga acacggagat aaagtcaagg
     6001 gtacggactg aaaaaaccgc acagcgaagc tgctcaggat tttttcggat ccggacttgc
     6061 gaagcccttg actttggcgg agtgtggagt tacctttgca tcgggtaaac gggaaaaatc
     6121 aaagtatatc cagttctgct atcatcttct ttagaactgg cacacttacg gtagagtagt
     6181 ccgacttgtc atagacatca acgagataca cacgaccttc actttcggaa gaacatatcg
     6241 taaacgtgat gacacgtgcg ccccctgact tgccttttcc tttggagact atggcaaggc
     6301 ggattttacg caaaccggga ctaagttcag tgcctatttt cgggtctttg gctaaatctt
     6361 cgacaagctc ctttaaatcg gacttgaaag acttgtacct tttcgcaagg attttcgctt
     6421 ccctctcaaa atgcggagtg gtctttactt caaagctcat cgaggaatgc gtttgcgttc
     6481 ttgcccggaa tttcgccgga ttcaattcgt tttacatcat tcaacccctc tttgatacgg
     6541 gttgcgattt tctcctccgc tgatacagga accagtttga aatctccgag acgagaggtc
     6601 aggaatacgg attccccctg tatggcacgg agaagggttg cggtctgatt tgttctaaac
     6661 ttgcttaatg aaactacttg catatctatt cattttttaa ttgtgttaca aatgtaacca
     6721 aagttactga tattgtaacc aaattaagtt tctttaacac gcgggaaagt tcgttccaaa
     6781 ctttaacctc taaccttccc caaaccagtc tttttttctt ccctattgtt tttccgaaag
     6841 tatgtttttg ggcagttttt gagtcacttc gagactaaaa ttctgtaacc ttgtcctaag
     6901 gttattatct tgtattaacc ttgtacaaaa aaaaatacgt ttacaattta ttggttttca
     6961 atatcataca gacaacaaaa aagccgatat acggaagaat agtacgtcat atacggaaga
     7021 atagtacgtc actttttttt ttatatacgg aagaatagta cgtcaataac gaaaaatcaa
     7081 cattattttt ccgtatatta ttttttatgc tatatttgca actgaaaacc aataattgag
     7141 agt
//
