LOCUS       NZ_CP072252             2782 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Bacteroides cellulosilyticus strain CL06T03C01 plasmid
            pBcelCL06T03-1, complete sequence.
ACCESSION   NZ_CP072252
VERSION     NZ_CP072252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16451184
            Assembly: GCF_018292125.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides cellulosilyticus
  ORGANISM  Bacteroides cellulosilyticus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Garcia-Bayona,L., Coyne,M.J. and Comstock,L.E.
  TITLE     Mobile Type VI secretion system loci of the gut Bacteroidales
            display extensive intra-ecosystem transfer, multi-species spread and
            geographical clustering
  JOURNAL   PLoS Genet 17 (4), e1009541 (2021)
   PUBMED   33901198
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Coyne,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) Department of Medicine/Division of
            Infectious Diseases, Brigham & Women's Hospital, 181 Longwood
            Avenue, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-OCT-2020
            Assembly Method                   :: FALCON-Unzip v. 1.2.0
            Assembly Name                     :: BcelCL06T03C01_pbv2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 171x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018292125.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 07:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,614
            CDSs (total)                      :: 5,535
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 25 of 79
            Pseudo Genes (incomplete)         :: 53 of 79
            Pseudo Genes (internal stop)      :: 15 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072252.1.
            Sequencing by the Institute for Genome Sciences, Genomics Resource
            Center, School of Medicine, University of Maryland, 801 W.
            Baltimore Street, Baltimore, MD 21201, USA. Gene calling method:
            Prodigal version 2.6.3. Annotated using a custom modification of
            Prokka version 1.14.6.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bacteroides cellulosilyticus"
                     /mol_type="genomic DNA"
                     /strain="CL06T03C01"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:246787"
                     /plasmid="pBcelCL06T03-1"
                     /geo_loc_name="USA: Boston"
                     /lat_lon="42.3361 N 71.1075 W"
                     /collection_date="2009-05"
     gene            1..1104
                     /locus_tag="INE78_RS27395"
                     /old_locus_tag="INE78_05478"
                     /db_xref="GeneID:66310435"
     CDS             1..1104
                     /locus_tag="INE78_RS27395"
                     /old_locus_tag="INE78_05478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_005783389.1"
                     /db_xref="GeneID:66310435"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYFDTVGQKMQTKAAPIREGVIVIKQETTMQELQQFAAVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASEALGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDTLKAKIKALEGENEQLSDRLGKAILEKERNGTKVFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="plasmid recombination protein"
     gene            1575..2165
                     /locus_tag="INE78_RS27400"
                     /old_locus_tag="INE78_05479"
                     /db_xref="GeneID:66310436"
     CDS             1575..2165
                     /locus_tag="INE78_RS27400"
                     /old_locus_tag="INE78_05479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008670455.1"
                     /db_xref="GeneID:66310436"
                     /translation="MENKKVLKLTDFQKNEENPFMKQAIEGIENHVVKKYKSSTGSDKK
                     AVVAVADTDTGEVFKTSFIRQIEVDEEQFAKLYLANFAAFFNLSQSAIRVFGYILTCMK
                     PKNDMIIFDRRKCLEYTQYKSDKAIYKGLAELVQSEIIARGPNEYNWFINPLIVFNGDR
                     VSFTKTYVRKKTLAAQKKEEAEKRQLSLGFDEL"
                     /name="hypothetical protein"
     Typing          1576..2155
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.479"
                     /coverage="95.0"
                     /evalue="2.71e-121"
     Typing          2..1101
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.64"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggagcaa caagtattca tgtacaagca gtgaagccgg ggagcgagat tcacaacttt
       61 agggaaaaag agttggacta tgttcgtccg gaacttagtc atttgaatga aagctgggtt
      121 ggagatagca tctcccatcg gctggagagt gcgaaacaga gatatttcga tacggttggg
      181 cagaagatgc agactaaagc agcacccata cgggaggggg taatagtaat caagcaagaa
      241 accaccatgc aggaactcca gcaattcgct gcggtctgca aggaacgttt cggtattgaa
      301 gcgtttcaaa tccatataca caaggacgaa ggatacatga acgcaaagca atggacacct
      361 aaccttcacg cccatgtagt tttcgattgg acgcagccga acgggaagag tgtgcgttta
      421 tcgcgtgatg acatggcaga actccaaacc atagcatctg aagcactggg catggaacgt
      481 ggtgtttctt ctgatcgcaa acacttatcg gctatgcagt acaagaccga atgtgcgaaa
      541 gaacagcttc aggaactatc caacgatata tccagtgcgt tagacaagca caaggacgtg
      601 caaaaccagc ttcttcagct tcagaaagaa ctacgttcca ttgaaacaaa gaaaaacgtc
      661 caaaaactca tttctaaggc ttcagagaag ttttacggct tgatagggaa aacggttaac
      721 gatagggaaa aagatacctt aaaagccaaa ataaaggctt tagaggggga aaatgaacag
      781 ctatccgata gactgggaaa ggctatactt gaaaaagagc gaaacggcac aaaggtattc
      841 aaagccgaga acgacaagga gtattataga cagcaaatgg acaatgcaag gacaaccagt
      901 aaccgactaa gaacggagaa ccaagaacta aagaccgaaa ccaaggaatt gaaaaaagaa
      961 cttggtaaga tgaaagactt gttcaattcc gaacaactgg aggctttaag gcatcatttc
     1021 ccaaacatat caaaagctat ggaagagggg aaagacctac tcaagcaaat caccagaagc
     1081 agaggtttcg gtatgggtat gtaacccccc ccgcccccaa agggggagcg accaaacggc
     1141 agcttcactc aatggagtgt tacgccgttc gggttcggaa gaaattttgc agggttcgaa
     1201 aaacggttgt gtgcttattc gaaaaaaagt ttatggtgtg ggcgctcgtc gggacgggtg
     1261 gtcccgcccc ttgccgctgg gcgctccccg accgatgatt ttttaagtag ttgattatat
     1321 gccgttttta gggtataata agaacttaag aaatagaata agttaagtag ttgatactca
     1381 atatagggta ttttccatat tggaaaatca gattttccat attagaaagt cactctttcc
     1441 tattagatgt taaaaaggaa aatatatttt gcttttttat tgtttaatgg aaatttaaat
     1501 attcctttgt ggaaaatcaa attatacttt ttcaccgata tatagaaagt caaatttgcc
     1561 taaatagaat gtttatggaa aataagaaag tactcaaatt aaccgatttt caaaaaaacg
     1621 aagaaaaccc ctttatgaaa caagcaattg aagggattga aaatcacgtt gttaagaaat
     1681 acaaaagtag tacgggaagt gataaaaaag ctgtagttgc tgttgctgat acagatacag
     1741 gagaagtatt caaaacctcg tttatccgac aaatagaagt agatgaagag caatttgcaa
     1801 aactatacct tgcgaatttt gccgcttttt tcaatctatc acaatcagct attcgggttt
     1861 tcgggtatat tctaacttgc atgaagccta agaatgatat gataattttc gataggcgaa
     1921 aatgcttaga atacacccaa tacaaatcag acaaagccat atacaaagga ttggcagagc
     1981 tcgtacaaag tgaaatcata gcaagaggtc caaacgaata taattggttc attaatccgt
     2041 tgattgtctt taatggggat agggtttcat ttacaaaaac atacgttcgg aaaaagactt
     2101 tagctgccca aaagaaagaa gaagcagaga aacgacagtt atcacttggt tttgatgaac
     2161 tgtaacactc cattgagtga agctgccgtt tggtcgctcc ccctttgggg gcgggggggg
     2221 atagataaag ttcctctatg taaagttata atgggggatg aaaggcaagg ttcgctaacc
     2281 ttaccccgat tgtttatgct tcccagcatt caatctatcc ccgtttatgg gtttctcttt
     2341 caccctacaa agataacctc atgggggaaa aatgtccaag aatatggggt aaaaactatc
     2401 aagtcggtag aaaataagta tctttgaagt acatttttag tagaggtact ggtatgccta
     2461 gacgaagcag ataggcaaaa ataaagccgc tggggatgtc ggatgtccgt caattaactc
     2521 cagcggcttg tattttggta actctcccgt atcggtcagc taccgtttgg tcagcaacaa
     2581 aggtagtact ttattgtcgc aaatccaaat gttttcccct ttatttgtgc atttacaaaa
     2641 cattcactaa cttcgcacct atgcagtatg gtgatagcac actagcaccc taaagggtac
     2701 gtgtgtaagg ggacaagccc ccttaacccc ctgtcagccg gctgtcaccg gctgttatcg
     2761 attgtcacaa aaatgttaac tt
//
