LOCUS       NZ_CP072235             7417 bp    DNA     circular CON 04-APR-2024
DEFINITION  Phocaeicola vulgatus strain CL06T03C24 plasmid pBvulCL06T03-1,
            complete sequence.
ACCESSION   NZ_CP072235
VERSION     NZ_CP072235.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16451191
            Assembly: GCF_018289355.1
KEYWORDS    RefSeq.
SOURCE      Phocaeicola vulgatus
  ORGANISM  Phocaeicola vulgatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Phocaeicola.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Garcia-Bayona,L., Coyne,M.J. and Comstock,L.E.
  TITLE     Mobile Type VI secretion system loci of the gut Bacteroidales
            display extensive intra-ecosystem transfer, multi-species spread and
            geographical clustering
  JOURNAL   PLoS Genet 17 (4), e1009541 (2021)
   PUBMED   33901198
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Coyne,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) Department of Medicine/Division of
            Infectious Diseases, Brigham & Women's Hospital, 181 Longwood
            Avenue, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-OCT-2020
            Assembly Method                   :: FALCON-Unzip v. 1.2.0
            Assembly Name                     :: BvulCL06T03C24_pbv2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018289355.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:39:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,331
            Genes (coding)                    :: 4,209
            CDSs (with protein)               :: 4,209
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 63 of 122
            Pseudo Genes (incomplete)         :: 65 of 122
            Pseudo Genes (internal stop)      :: 18 of 122
            Pseudo Genes (multiple problems)  :: 19 of 122
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072235.1.
            Sequencing by the Institute for Genome Sciences, Genomics Resource
            Center, School of Medicine, University of Maryland, 801 W.
            Baltimore Street, Baltimore, MD 21201, USA. Gene calling method:
            Prodigal version 2.6.3. Annotated using a custom modification of
            Prokka version 1.14.6.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Phocaeicola vulgatus"
                     /mol_type="genomic DNA"
                     /strain="CL06T03C24"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:821"
                     /plasmid="pBvulCL06T03-1"
                     /geo_loc_name="USA: Boston"
                     /lat_lon="42.3361 N 71.1075 W"
                     /collection_date="2009-05"
     gene            1..978
                     /locus_tag="INE85_RS22085"
                     /old_locus_tag="INE85_04452"
     CDS             1..978
                     /locus_tag="INE85_RS22085"
                     /old_locus_tag="INE85_04452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669318.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_008669318.1"
                     /translation="MEKKDKKQRDIVLSQDNALTTARYKFDLIEKRALYSIIRNIRAQY
                     IENENGRRDLFDDLIITIHEEELKKCGDNTKMQNIYDALKRLRNRDIEINNEKMWLSIG
                     FVNYVKHIKNKSYFEVQVSKEVLPYYVELAQNFTSYSMTVAIALKSTFSQRFYELCCQY
                     RNAGFFFYTVEKLREMFMLEKKYKRGCDFKRRVLDDPQKELRELYNAGQCDLYFEFEPK
                     SYKGKEVTEYKFYVYTRQTEQQKLLEYESAKQAIIYITTTISRFSKSDKGYVKRVVNAV
                     QLHPEIAVQVAQKIQKKFEQYIDKPAKQIGAIIRVCLMEDFGIE"
                     /name="replication initiation protein"
     gene            991..1182
                     /locus_tag="INE85_RS22090"
                     /old_locus_tag="INE85_04453"
     CDS             991..1182
                     /locus_tag="INE85_RS22090"
                     /old_locus_tag="INE85_04453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008782581.1"
                     /translation="MKTGDKVIVPAEINGYGRDLRAMVTEIEKFAGAIFVTVIFTEPCP
                     EACGRKGGVYHDFQLITE"
                     /name="hypothetical protein"
     gene            1285..1806
                     /locus_tag="INE85_RS22095"
                     /old_locus_tag="INE85_04454"
     CDS             1285..1806
                     /locus_tag="INE85_RS22095"
                     /old_locus_tag="INE85_04454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_008782582.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAQKLDEYLKGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVAGRLEQSAKIMEQHNEETEAVRLLLQTVREQTAK
                     QLPAPELIAHAAEEKAKAEAKSEEQEREIARLRSENSALLEYKEKAHRELCRVRDEQKT
                     IGKIKVNTEL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            1806..>2180
                     /gene="dcm"
                     /locus_tag="INE85_RS22100"
                     /old_locus_tag="INE85_04455"
                     /pseudo=""
     CDS             1806..>2180
                     /gene="dcm"
                     /locus_tag="INE85_RS22100"
                     /old_locus_tag="INE85_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015193646.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /name="dcm"
     gene            complement(2346..3179)
                     /locus_tag="INE85_RS22105"
                     /old_locus_tag="INE85_04456"
     CDS             complement(2346..3179)
                     /locus_tag="INE85_RS22105"
                     /old_locus_tag="INE85_04456"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032951959.1"
                     /translation="MILPTMTLTELAKEIQSDYKEVHARWTKFNPKFNKMRLKQTYYPW
                     IWNTEIITKKNNKWFFSFYAQSKEDANVVIPHAYITFRYGGTTWAAYPLKGTNVLLIFS
                     SHFFERYIERFLELNKDEKQYTSLDIIKLFYLRNNHIGCIKPELEDLARGFCEDGMILG
                     EWISESAALIKTFLSRNELKVNQYTEYYDMFHNWIIQDMFLAHKGYSLSSMEEFDNLPD
                     SYFSPKEWEKFLKLRGNEMWINIAIECSEFRIEHKEEYEKCSNMLQAIMDNRSLE"
                     /name="hypothetical protein"
     gene            complement(3211..4227)
                     /locus_tag="INE85_RS22110"
                     /old_locus_tag="INE85_04457"
     CDS             complement(3211..4227)
                     /locus_tag="INE85_RS22110"
                     /old_locus_tag="INE85_04457"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008782585.1"
                     /translation="MEKQTAVRETLLKEFANCSDKLFTLGIIRTDSFTGEIGEFIASKY
                     FKLSLAGKSTKAYDGVCPKGYKYQIKSKVISNNNLTHHISNLKYQDFDYLVVVYFDIYY
                     NPISILKIPSNKINTEEYIIGASSVHSFSQNIARLKLLQKEQVAIRNFAQSYLNLQKEG
                     IIRSRKVVGDIGEYYACKRLNLKLSSNKNEKGLDAIGQGGLTFEIKTRRVYDSERRTSE
                     TRRINNLIGKNADYLIVVTLNHAFECSGMWIMPMKNIINPKSANLKIVNTTKGVKNLVP
                     SQISWLNTGEKFVSFNCMDKQNNSQVEVTNSDIKGNSNKMRIILIIIIIFAIICLVV"
                     /name="hypothetical protein"
     gene            complement(4402..5280)
                     /gene="btgB"
                     /locus_tag="INE85_RS22115"
                     /old_locus_tag="INE85_04458"
     CDS             complement(4402..5280)
                     /gene="btgB"
                     /locus_tag="INE85_RS22115"
                     /old_locus_tag="INE85_04458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgB"
                     /protein_id="WP_008782579.1"
                     /translation="MNVKIQGGGNGTYANTGSCVAVTNYLQHEDLERMKKGEEVQPFFN
                     QFRDYVSSREVTFKIDNNKAKLSQTDAKFYVITVSPSEKELRCMGRTPQERAEALQWYI
                     RQDVMRNYAEGFGKGLRSDDVEYYAKIHFNRDGDSDSDMHAHIIVSRKDRSNTKKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDRRMGFDREPEESFDYLNAVKNGSPAEIAR
                     QVERAERIRKEKWDNLKAELQSRQEPEKSKIQQVEKTPDIEQPIPKKKQQEEDLELLKK
                     PKRSRGFGMGM"
                     /name="btgB"
     gene            complement(5277..5882)
                     /gene="btgA"
                     /locus_tag="INE85_RS22120"
                     /old_locus_tag="INE85_04459"
     CDS             complement(5277..5882)
                     /gene="btgA"
                     /locus_tag="INE85_RS22120"
                     /old_locus_tag="INE85_04459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgA"
                     /protein_id="WP_008782580.1"
                     /translation="MDKETTTSVTIDRKTFARLDRLAKSNNVSKKDFLSCALEYFEKYG
                     INPVEHESPAKEMQKLIKRCDQVIAFIRKQEQDFLRPACEAMGSTSMRVTMSMDSILTE
                     KKFSQYQKDNDLFMRDLASLAGIREQALDRTEKAVGQSRDMLLKNQQAIYARLDAVTQR
                     QEKIFSYIASYIDAKGKAGLFDDIKALYKDEKNKREKI"
                     /name="btgA"
     gene            6275..6622
                     /locus_tag="INE85_RS22125"
                     /old_locus_tag="INE85_04460"
     CDS             6275..6622
                     /locus_tag="INE85_RS22125"
                     /old_locus_tag="INE85_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_008669299.1"
                     /translation="MEAKSRFKVKLSNEAQEFLDGLPNKVREKIISNIRKSTFVLDPDL
                     FKKLDDTDIWEFRTFYNKTKYRLLAFWDKDDKINTLVIATHGFIKKTQKTPPKEIAKAE
                     EIRKDYFNSKR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6628..6942
                     /locus_tag="INE85_RS22130"
                     /old_locus_tag="INE85_04461"
     CDS             6628..6942
                     /locus_tag="INE85_RS22130"
                     /old_locus_tag="INE85_04461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_007851388.1"
                     /translation="MEAKKFYTLEEIEDKYIGEKGTPKRDAYEEELNSFLIGEAIKQAR
                     QSKNLTQEELGNLIGVQRAQISRIENGKNLTFSTIARVFKAMGISAKLEIGSMGKVALW
                     "
                     /name="helix-turn-helix transcriptional regulator"
     Typing          2..978
                     /name="rep_cluster_1097"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.773"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4406..5280)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.233"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggagaaga aagacaagaa acagagggat atagtcctgt cgcaagacaa tgcgctgact
       61 accgcacgct acaagttcga ccttatcgaa aaacgtgccc tatactccat tatacgaaat
      121 atccgggcgc aatacatcga gaacgagaac ggcagacggg acttattcga tgatctgatt
      181 atcaccatac acgaggaaga actaaaaaaa tgcggtgaca ataccaagat gcaaaacatc
      241 tacgatgcgc taaaacggct tagaaatcgc gatatagaaa ttaacaacga aaagatgtgg
      301 ctaagtatcg gttttgtcaa ttacgtcaag catatcaaaa ataaatcgta ttttgaggtg
      361 caagtttcca aagaggtact accctattac gtggaactcg cccaaaactt cacgagttac
      421 tccatgacgg tagcaatcgc actcaaaagc acattctcgc aaaggtttta cgaactgtgc
      481 tgccaatacc gaaacgccgg gttcttcttc tacacagtcg aaaagctccg ggaaatgttc
      541 atgttggaaa agaaatacaa acggggatgc gatttcaaaa ggcgtgtact tgacgatccg
      601 caaaaggaac tccgggagtt gtacaatgcc ggacaatgcg atttatattt cgagttcgaa
      661 ccaaagagct acaaaggcaa ggaagtgacc gaatacaaat tttacgttta cacaaggcaa
      721 accgaacaac aaaagctatt ggaatacgaa agcgccaaac aggcgatcat ctacataaca
      781 accactattt cgaggttctc caaaagtgac aaaggatatg ttaagcgtgt agtgaacgcc
      841 gtacagttac acccggaaat agccgtacag gtggcgcaaa agatacaaaa gaagtttgag
      901 caatatatag acaagccagc caaacagata ggggctatca tccgggtttg cttaatggaa
      961 gattttggaa tagaataaaa aaatacaagt atgaaaacag gtgataaagt tatcgtacca
     1021 gccgaaatta acggctatgg cagggattta cgggcaatgg ttacagagat agagaagttc
     1081 gccggggcaa tattcgtaac agtgatattc actgaaccat gtccggaagc ctgcggaaga
     1141 aaagggggcg tttaccatga cttccagcta ataacagagt gatttatttt gctataaccg
     1201 caataaattg caataacatt tgcgataagc tgcaataaca aaacttataa ctacctttgc
     1261 aacagaatat agcattttac aacaatggaa aaaggacaag aaagcaagca aaagaggaaa
     1321 acaccgctta ccaccatagc gataagccaa gagtacgccc aaaaattaga tgaatattta
     1381 aagggtacgg gcataacccg caaagaattt gttgagttgt ccgttgatta tttccaaaga
     1441 accgggttcg acctccgggg tgaagcattt gacctctccc cattagaaaa ggttgccggg
     1501 cggctggaac aatccgcaaa gatcatggaa caacacaacg aggaaacgga agcggtgcgc
     1561 ctgcttctcc aaactgtccg ggaacagacc gccaagcaac tccctgcgcc ggaactgatc
     1621 gcccatgctg ccgaagaaaa agcaaaggca gaagccaaaa gcgaagaaca agaacgggag
     1681 attgcccggc tgcgctccga aaactccgct ttgctagaat acaaggaaaa ggcacaccgg
     1741 gaactgtgcc gggtacgtga cgaacaaaaa acaataggga aaataaaagt aaacaccgaa
     1801 ttataatgaa aggaactatt aaatttatag acctctttgc tggaattggt ggcattcgat
     1861 gtggcttaga attagccgca cacgaagctg gttataaaac agagtgtgta tttacttctg
     1921 aaatcaagaa acatgccata aaggttttaa aacaaaatca tcctaacgag ttaataaacg
     1981 gagatattac acaagttaat gaaaaagaaa ttccagattt tgacatttgt tgtgcaggtt
     2041 ttccatgcca atctttttcc ggcggcggaa aaagattggg ctttacagaa acacgtggta
     2101 ctttgttttt tgatgttgaa agaattttaa agaaaaagga acctgctggt tttatcctcg
     2161 aaaacgttga aggacttgta tcattcatat taacaaatct tgcaaccaaa gatgaaaacc
     2221 attaactgag gcatattgac ctaccatgtc tatgtagtta atcgcatacg acaagtccac
     2281 ttcataacca taaaaatcta ttaagtcttg tattaattct tctggggtaa tcataatgtt
     2341 actttttatt ctaaagaacg attgtccata atcgcttgaa gcatattaga acatttttca
     2401 tattcttctt tatgttcaat tctaaattcg ctacattcaa tggctatatt tatccacatt
     2461 tcattacctc ttagtttaag aaatttttcc cattctttag gcgagaagta ggaatctggt
     2521 aaattatcaa attcttccat agatgagaga gaatatcctt tgtgagctaa gaacatatct
     2581 tggataatcc aattatgaaa catatcgtaa tactctgtat attgatttac ttttagttca
     2641 ttgcgagata agaaggtttt gattaatgca gcagattcag aaatccattc acctaaaatc
     2701 attccatctt cacagaaacc tctagctaaa tcttctaatt caggttttat acatccaatg
     2761 tggttattgc gtaaataaaa taattttatt atatcaaggg atgtgtattg cttctcatcc
     2821 ttatttagtt ccaagaaacg ttcaatgtaa cgttcaaaga aatgggaaga aaaaatcagt
     2881 aacacatttg tccctttaag cggataagct gcccatgtag ttcctccata cctaaaagta
     2941 atgtaggcat gaggaattac aacgttagca tcctctttag attgtgcata aaagctgaag
     3001 aaccatttat tatttttctt tgtaataatc tctgtattcc atatccatgg ataatatgtt
     3061 tgcttcaatc tcattttatt gaatttggga ttaaatttag tccagcgtgc atggacttcc
     3121 ttgtaatcag actgaatttc ctttgccaat tcggtcaatg tcattgttgg taaaatcata
     3181 ttaacaaata tttagaattg agattaataa tcacacaacc aaacatatta tagcgaagat
     3241 aatgattata attagaataa tacgcatttt attgctgttt cccttaatat cagaatttgt
     3301 gacttccact tgagagttat tttgtttatc catgcaatta aatgatacaa acttctcgcc
     3361 agtgtttaac caactaattt ggcttggtac taaattcttt actccttttg ttgtattaac
     3421 aatcttaaga ttggctgatt taggattaat tatatttttc attggcatta tccacattcc
     3481 agaacattca aaggcatgat ttaaagttac cacaattaaa taatctgcat ttttgcctat
     3541 tagattgttt attcttcttg tttcagaagt ccgtctttca ctatcatata ctcgccttgt
     3601 tttgatttca aaagttaatc ctccttgacc tattgcatcc agtcccttct cattcttatt
     3661 gctagataat tttaggttca agcgtttaca agcataatat tctccaatat cccctactac
     3721 tttacggctt ctaataatcc cttctttttg taaattcaag taactttgag caaaatttct
     3781 gatagcgact tgttcttttt gaagtaattt taatcgagct atattctgag aaaatgaatg
     3841 cacagaagaa gcgccaataa tgtattcttc cgtattaatt ttattagaag ggatttttaa
     3901 tatggatata ggattgtaat atatatcaaa atatacaact acgagatagt caaaatcttg
     3961 atattttaga ttagaaatat gatgagtaag attattgtta gatataactt tagatttaat
     4021 ttgatattta tagcctttag gacatactcc atcgtatgct tttgtggatt taccagctaa
     4081 acttagttta aaatacttgg aggcaataaa ttctcctatt tctccagtaa agctatctgt
     4141 acgaattatt cctaaagtga aaagtttatc actgcaattg gcaaattctt ttaataaagt
     4201 ttctctaact gctgtttgct tttccatgaa taaaatttta ttgttttatt aatggctata
     4261 aaggtagaaa taaataaagt atttagcaaa ttttggattc cgctaattgt tgtgcgatgg
     4321 tcgcccagtt ggctatgggc atggtccatt tcttacgtat gttgcggtaa gcgagataaa
     4381 cggtgccgga aaatagaaaa atcacatgcc cattccgaaa cctctgctcc tttttggctt
     4441 cttcaggagt tccagatctt cctcctgctg cttctttttg ggaataggct gctcgatgtc
     4501 cggcgttttt tccacctgct gaattttcga cttctccggt tcctgccggg attgaagctc
     4561 cgctttgaga ttgtcccatt tctcctttct gatgcgttcc gcacgttcca cctgccgggc
     4621 tatttccgcc gggcttccgt tcttgacggc attaagatag tcgaagcttt cctccggctc
     4681 ccggtcaaac cccatgcgcc gatcaaaaga gctttcacac ttccggaaaa aatccgtccg
     4741 gtcaaaacct cccttcacgt tgccgcagtt cttctttccg gtgtggttcg ttttagggct
     4801 taactttttg gtgttgctcc ggtctttccg actgacgatg atatgtgcgt gcatatcgct
     4861 gtcactgtca ccgtcccgat tgaaatggat ctttgcgtaa tattccacat catcgcttct
     4921 caagcctttt ccgaagccct ccgcatagtt tctcatcacg tcctgccgga tgtaccactg
     4981 caaggcttcc gcacgctcct gcggagtcct gcccatgcag cgcagctctt tctctgaagg
     5041 gctgacggta ataacataga actttgcgtc agtctggctt aacttagcct tgttgttgtc
     5101 tatcttgaat gtgacctccc ttgagctcac gtagtcccgg aactggttga agaacggctg
     5161 cacctcctcc cctttcttca tcctctccaa atcctcatgc tggagatagt tcgtcaccgc
     5221 aacacaactg cctgtattgg cgtatgtgcc gttaccgcct ccctgtatct tcacgttcat
     5281 atcttttccc ttttgttctt ctcgtccttg tacaaggctt ttatatcatc gaaaagcccg
     5341 gcttttcctt tcgcatcgat gtagcttgcg atataggaga agatcttctc ctgcctttgc
     5401 gtcacagcgt ccagccttgc ataaatggct tgttggttct tcagaagcat atccctcgac
     5461 tgaccgactg ccttttccgt ccggtccaaa gcctgctccc ttatcccggc aaggcttgca
     5521 agatcacgca tgaataggtc attgtccttt tggtattggc tgaacttttt ttccgtaagt
     5581 atcgagtcca tactcatggt cactctcatg cttgtactgc ccatggcttc acaagccgga
     5641 cgcaagaaat cctgctcctg cttcctgatg aacgctatca cctgatcaca gcgtttgata
     5701 agtttctgca tttcctttgc cggactctca tgctcaaccg ggttgattcc gtacttctcg
     5761 aagtactcca gcgcacagga aaggaagtct ttctttgata cattattgga ctttgccagc
     5821 ctgtcaagcc gggcaaacgt ttttcggtca atggtgacag aagtagtggt ttccttatcc
     5881 atacaattgc tgttttaatt gctaattata ttattaaatc tctgatttac aaaggtatga
     5941 ttttaaccac aatttcaaac taattgcggt taaaaaactt aaacggctga tattcagccg
     6001 taaccccgta gggcaagagg aaaaacaggg caaagcccgg tttcctcttg ctgttctttc
     6061 gactgccgag caatcgaaag aacaccctcc cctgcggtac ggcgaaagct cgtacaaggg
     6121 ggacggggag aagtcggagc gaggcactaa gaaagaacct attccacaaa caaaaaaaca
     6181 cattaaaacg gcacaacagg ctgcataaaa taaatatatt ttgtttccat ataatgaaac
     6241 aaaatattat ctttgcaaca ctaaaaaact ttatatggaa gcaaaatcaa gattcaaggt
     6301 caaattatcc aacgaagccc aagagttttt agacggattg cccaacaagg tacgtgagaa
     6361 gatcattagc aatattcgta aatccacctt tgtacttgat ccagatttat tcaagaaatt
     6421 ggatgatacg gatatttggg aattcagaac gttctataac aaaaccaagt acaggcttct
     6481 tgctttttgg gataaggacg ataagataaa cactctggta atcgcaacac atgggtttat
     6541 caagaaaacc caaaagacac ctcccaaaga aatagccaaa gccgaagaga ttagaaaaga
     6601 ttattttaat tcaaaaagat aaagcgtatg gaagcaaaga aattttatac actcgaagaa
     6661 atcgaggata aatacatcgg agaaaaaggt actcccaaac gtgatgccta cgaagaagaa
     6721 cttaactctt ttttgatagg tgaagccatt aaacaggctc gccaatcaaa gaacttaacc
     6781 caagaggaat taggtaatct gataggagtg caacgagctc aaatctcccg aattgaaaac
     6841 ggtaagaatt taaccttttc caccattgca agagttttta aggctatggg gataagtgct
     6901 aaacttgaaa tcggaagcat gggtaaagtt gcgttatggt aactaactat acaacaaaaa
     6961 attacattaa atccgggatg cgttttcgct cccggaaact tctcgtttta tacatttaaa
     7021 gtcacattta tacaaaattt aacacgccag aaaatcttcc taatccgttg cacttggctt
     7081 tacctgtttc cactctctcc cattctccgg aaattcgtcg tatcaaaaaa cacgctgaaa
     7141 agtggagcga agcgacctct ataaaccaaa aaagaaacac tccattgctt tacggtaatt
     7201 tttagtttct ttttagttac ttttatggca tagcataaca cattgtgcta taatatctta
     7261 caaaggtcat ctacgacaaa tttccgagca tctacgacaa atttccgagc atctacgaca
     7321 aatttccgaa aaaaacggct atctacgaca aatattctac gtcaaatatt tgtttttgtc
     7381 gtagataaat atttcctttg cgaaaagcat taaagtc
//
