LOCUS       NZ_CP072232             9518 bp    DNA     circular CON 03-APR-2024
DEFINITION  Parabacteroides distasonis strain CL06T03C10 plasmid
            pPdisCL06T03C10-1, complete sequence.
ACCESSION   NZ_CP072232
VERSION     NZ_CP072232.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16451192
            Assembly: GCF_018289335.1
KEYWORDS    RefSeq.
SOURCE      Parabacteroides distasonis
  ORGANISM  Parabacteroides distasonis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
            Parabacteroides.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Garcia-Bayona,L., Coyne,M.J. and Comstock,L.E.
  TITLE     Mobile Type VI secretion system loci of the gut Bacteroidales
            display extensive intra-ecosystem transfer, multi-species spread and
            geographical clustering
  JOURNAL   PLoS Genet 17 (4), e1009541 (2021)
   PUBMED   33901198
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Coyne,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) Department of Medicine/Division of
            Infectious Diseases, Brigham & Women's Hospital, 181 Longwood
            Avenue, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-OCT-2020
            Assembly Method                   :: FALCON-Unzip v. 1.2.0
            Assembly Name                     :: PdisCL06T03C10_pbv2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 366x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018289335.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 05:41:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,428
            Genes (coding)                    :: 4,307
            CDSs (with protein)               :: 4,307
            Genes (RNA)                       :: 107
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 69 of 121
            Pseudo Genes (incomplete)         :: 50 of 121
            Pseudo Genes (internal stop)      :: 20 of 121
            Pseudo Genes (multiple problems)  :: 15 of 121
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072232.1.
            Sequencing by the Institute for Genome Sciences, Genomics Resource
            Center, School of Medicine, University of Maryland, 801 W.
            Baltimore Street, Baltimore, MD 21201, USA. Gene calling method:
            Prodigal version 2.6.3. Annotated using a custom modification of
            Prokka version 1.14.6.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Parabacteroides distasonis"
                     /mol_type="genomic DNA"
                     /strain="CL06T03C10"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:823"
                     /plasmid="pPdisCL06T03C10-1"
                     /geo_loc_name="USA: Boston"
                     /lat_lon="42.3361 N 71.1075 W"
                     /collection_date="2009-05"
     gene            1..960
                     /locus_tag="INE86_RS21760"
                     /old_locus_tag="INE86_04434"
     CDS             1..960
                     /locus_tag="INE86_RS21760"
                     /old_locus_tag="INE86_04434"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_113748955.1"
                     /translation="MENKPIMLAQANALTQARYDFNPIEKRCLYQIIREVRRLFIDSNT
                     GQRDLFNNMRVKLTPDLLAECSDKSNIAKVYESLKRLRKRDVEIDNEDVWMNTGYVTMV
                     KHDKRANIYEVEVSSEIMPYLVALAENFTSYDLTVAISLKSAYSQRFYEYCCQFKNRTN
                     KTFFFSVDDLKKMMMLEDKKGYDNSSNFKTKVLDVAQKELKEAYDNGQSDLYFDYQVKD
                     KQGRKILSWFFFIHTKDEEQKTDYKTVGECIRQIHNILTTFFPRDSKYIKRVIAAVNLR
                     PDIAMELTEKMQKKVNDYSRKEIAPIIRYVLREDYNII"
                     /name="replication initiation protein"
     gene            1077..1694
                     /locus_tag="INE86_RS22855"
                     /old_locus_tag="INE86_04435"
     CDS             1077..1694
                     /locus_tag="INE86_RS22855"
                     /old_locus_tag="INE86_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_243704187.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAQKLDGYLNGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVTGRLEQSAKIMEQANEGTEAVRQLLQAVKEQTAI
                     QKQLPAPDLLQKATNRVAELEHLIGKYQEKLDSLSEDKTMIIKERVEWERKYYERDKQN
                     YALLDTLKKQGELLEKAKTELRHCKGFFSSANEDVLKELGIS"
                     /name="hypothetical protein"
     gene            2021..2347
                     /locus_tag="INE86_RS21770"
                     /old_locus_tag="INE86_04436"
     CDS             2021..2347
                     /locus_tag="INE86_RS21770"
                     /old_locus_tag="INE86_04436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113748957.1"
                     /translation="MEKTQRKQSLNFLSDTPKLFDLKELLSSLKITPGQMENMKGLIYI
                     EGLTEKQVRQKLSHSKIIRTNRFGVNSCLYYILFEDVKGRYLCLFNGDRECFRALFFSK
                     VLLN"
                     /name="hypothetical protein"
     gene            2524..2724
                     /locus_tag="INE86_RS21775"
                     /old_locus_tag="INE86_04437"
     CDS             2524..2724
                     /locus_tag="INE86_RS21775"
                     /old_locus_tag="INE86_04437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_113748958.1"
                     /translation="MEKANIGFKAGIILEKLGETGLITIAELARRLNFAADETALAAGW
                     LARENKIYIERRNGLLYIRKE"
                     /name="winged helix-turn-helix domain-containing protein"
     gene            2847..3083
                     /locus_tag="INE86_RS21780"
                     /old_locus_tag="INE86_04438"
     CDS             2847..3083
                     /locus_tag="INE86_RS21780"
                     /old_locus_tag="INE86_04438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_113748959.1"
                     /translation="MDKHIIRENAGKLWRLLSTDVHRKWNLQEVKELTGFNDLEVACAI
                     GWLAREDKIQFELEHHDTKDKNALLYLMLNVYF"
                     /name="winged helix-turn-helix domain-containing protein"
     gene            3441..3872
                     /locus_tag="INE86_RS21785"
                     /old_locus_tag="INE86_04439"
     CDS             3441..3872
                     /locus_tag="INE86_RS21785"
                     /old_locus_tag="INE86_04439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226766949.1"
                     /translation="MEKKETGLMRQIGYLSFVVVLLYGLTLFAVSCDNDEDPPLPPAVE
                     KVVLAFGVDQGEGQLSAKPELENTAAEKIGELISPAEIAKGTTVHFRATHSKSWTIMYW
                     KVNGVIIRSVEPEQTFVIDEHKEVRVAFKPYKSPEPEGL"
                     /name="hypothetical protein"
     gene            complement(3955..4260)
                     /locus_tag="INE86_RS21790"
                     /old_locus_tag="INE86_04440"
     CDS             complement(3955..4260)
                     /locus_tag="INE86_RS21790"
                     /old_locus_tag="INE86_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005783170.1"
                     /translation="MKKVLFAVALVMGLGSSVAFAGNMVSDVEIVVMVNDYKPIEVKDL
                     PQAVQDTIKKDFADLTIKEAAVEESEEGTKTYKVTFTDAEGTDSEVFFNEKGEVLK"
                     /name="hypothetical protein"
     gene            complement(4500..4658)
                     /locus_tag="INE86_RS21795"
     CDS             complement(4500..4658)
                     /locus_tag="INE86_RS21795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_211744817.1"
                     /translation="MLFFGGVSVLSFCYRDLSAWWETRPDQPDTQYHFVIAGELFLWCN
                     APILLSF"
                     /name="hypothetical protein"
     gene            complement(4839..6470)
                     /locus_tag="INE86_RS21800"
                     /old_locus_tag="INE86_04441"
     CDS             complement(4839..6470)
                     /locus_tag="INE86_RS21800"
                     /old_locus_tag="INE86_04441"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_113748950.1"
                     /translation="MKKGELAKKKRKGCLTALLVVWAVFMVKVISVLDLHFGDIWGRMK
                     AGDIAGFAYLWLVVFFVLLCCLVLWRIVPKGYFRKKQPDTAAAPSWAVTIPTHEGAVIV
                     GNPFRGIFIAGAAGSGKSESVAVPLLLEFIRKGFAGIVYDFKFPALDNDITAFINATGS
                     DLRHFCLNFSDPYRTDFVNPIKPEYVPNTSYAREYAVSIVANLTKESIKNPDFWSRSAT
                     DVLTACIWYLREEHPEICDIPHVLAMVGSNGTALLNTLQKNIITEQMVRSVYDALQRGA
                     DNQVSGVLGTLQGAVAQINTPEMMWVFSRDGCPLDLNDPEAPAVLTVATNPTTVQTLSP
                     LCSLVITVATKLMNQPGKAPSFVLLDEAPTIFIPHLETIPNTGRSNKIATVLMCQDLAQ
                     LADGYGDKKADVLFAACNSHFYGRVASSKTAEVFSRQFGKTDTVFTTRSENKGAISPFK
                     SKGQSEGVQERDIYRPSVFMELQVGEFIGRTVESATPVFHGHFKQVDSRCRQLVTEQPE
                     RFVLGSDVMDYYKQVREDIKSVLGQS"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(6467..7237)
                     /locus_tag="INE86_RS21805"
                     /old_locus_tag="INE86_04442"
     CDS             complement(6467..7237)
                     /locus_tag="INE86_RS21805"
                     /old_locus_tag="INE86_04442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="WP_113748951.1"
                     /translation="MNVKIQGGGGGTYANTGSCTGVVTYLQHEDLERIRQGQQVEPFFN
                     QYRDFVSPKEVTYKIDHNRAQLHKTDAKFFVITVSPSEKELAAMGATQEQQAQAMKQYI
                     RKDVIPAYAENFNKGLQADNIEYYAKIHFSRDKAGVTDMHAHIIVSRKDRSNTIKLSPK
                     TNHRNTGKGAVKGGFDRTEFFRKCETGFDLRTNYRRDVKESFEYLNAMKNGTPAEIKKQ
                     ASRAVQYQARREAEKEITNKVVKKIVKGIGGRGL"
                     /name="DUF5712 family protein"
     gene            complement(7244..7762)
                     /locus_tag="INE86_RS21810"
                     /old_locus_tag="INE86_04443"
     CDS             complement(7244..7762)
                     /locus_tag="INE86_RS21810"
                     /old_locus_tag="INE86_04443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_113748952.1"
                     /translation="MNEQTTVTIDRKTAVILDRLAKSNDVSKKEFLSCALEYFEKYGIN
                     PVQHESPAKEMQKLIKRCDQVIAFIRKQEQNILRPACAAISTTEARIESVLDKLATAGQ
                     VRDLQKAVSDQQQRQNYDARNLRNDILQLLGRATEQERKAFMYLGKLVDAKEKSGFFND
                     IATLYKEQG"
                     /name="BfmA/BtgA family mobilization protein"
     gene            8386..8733
                     /locus_tag="INE86_RS21815"
                     /old_locus_tag="INE86_04444"
     CDS             8386..8733
                     /locus_tag="INE86_RS21815"
                     /old_locus_tag="INE86_04444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_113748953.1"
                     /translation="MEEKAKFKIIYSKDADDFLNNLPTKIKDKIIYNIAKAKFVIDPEL
                     FKKLDDTDIWEFRTFYNKVKYRLLAFWDKDNGTNTLVIATHGFIKKTQKTPPKEIAKAE
                     DIRKAYFNSKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8739..9053
                     /locus_tag="INE86_RS21820"
                     /old_locus_tag="INE86_04445"
     CDS             8739..9053
                     /locus_tag="INE86_RS21820"
                     /old_locus_tag="INE86_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_121960625.1"
                     /translation="METMKFYTEEEILDKHIGKKGTPKRDQFEADLNSFLIGEAIKQAR
                     QSKNLTQEELGNLIGVQRAQISRIENGKNLTFSTIARVFKAMGISAKLEIGSMGKVALW
                     "
                     /name="helix-turn-helix transcriptional regulator"
ORIGIN
        1 atggagaata aacctattat gttggctcaa gccaacgccc taacacaagc aagatacgat
       61 tttaatccta tcgagaagcg gtgtctgtat cagatcatcc gggaagtccg caggctgttt
      121 atagacagca ataccgggca acgggattta ttcaacaata tgcgggttaa acttactccg
      181 gatctgctcg cagaatgtag cgacaaaagc aacatagcca aagtttacga atcccttaaa
      241 cggcttagaa aacgggatgt agagatagat aacgaggatg tgtggatgaa taccggatac
      301 gtcacaatgg taaagcatga caaacgggca aacatctacg aggtagaggt ttcttcggag
      361 atcatgccct atttagtcgc tttagccgag aatttcacaa gctacgatct gacggtggca
      421 atcagcctaa aaagtgccta ttcgcaacga ttttatgaat actgctgcca atttaagaac
      481 cgcacgaaca agaccttttt cttttccgtg gatgatctca agaaaatgat gatgttagag
      541 gacaaaaaag gttatgacaa ctcaagcaat tttaaaacaa aggttctcga tgtggctcaa
      601 aaagagctga aagaagcata cgataacggg caatccgatc tgtattttga ttaccaagtg
      661 aaggacaaac aagggcgcaa gatcctttca tggtttttct ttatccatac caaagatgaa
      721 gaacaaaaaa ccgattacaa gacggtcggc gagtgcatcc ggcaaattca taacatactg
      781 acaaccttct ttccacgtga cagcaagtat ataaaacggg tgatagctgc cgttaatctt
      841 cgcccagata tagcaatgga actgacggaa aagatgcaga agaaagtgaa cgattatagc
      901 cggaaagaaa tagcccctat catccggtat gtattgcgag aagattataa tatcatttaa
      961 tccccaaaaa gtgatttatt tcgctataac tgcaataaat cgcaataaaa agcgtaataa
     1021 attgcaataa ccaaacatat aactaccttt gcaacaacat atagcacttt atagcaatgg
     1081 aaaaaggaca ggaaagcaag caaaagagga aaacaccgct taccaccata gcgataagcc
     1141 aagagtacgc ccaaaaatta gacgggtatt taaacggtac gggtataact cgcaaagaat
     1201 ttgtcgagtt atccgttgat tacttccaaa gaaccgggtt cgatctccgg ggtgaagctt
     1261 ttgacctctc cccattggag aaggttaccg ggcggttgga acaatccgca aagatcatgg
     1321 aacaagcgaa tgaaggaacg gaagcggtgc ggcagcttct ccaagctgtc aaggaacaga
     1381 ccgcaataca aaaacaactc cctgcgccgg atctattgca aaaagcgacc aacagagtag
     1441 ccgaactgga acaccttata gggaaatacc aagaaaaact tgattccctt agcgaagata
     1501 aaacaatgat tataaaagag agagtagaat gggaacggaa atattatgaa cgggataaac
     1561 aaaactatgc ccttttagat acacttaaaa agcaaggcga actcttggaa aaagcgaaaa
     1621 cagagttaag acactgtaaa ggctttttct cgtccgcaaa cgaggatgtt ctaaaagaac
     1681 ttggtatttc ataaacactg aattttaaga taataaggca aaaaaataaa aggttcataa
     1741 acccttttag ccaacaattt gttatctttg tgcagtcgta aaccgactgt gtttttacat
     1801 attgaagaaa aaaagcgttt agctgatttc tgtgcaagcg aaaagtccaa aaattgcatt
     1861 atacacagag aaacagataa atgctccccg tgatatgcgg gggtatctcc tgtttgtgtg
     1921 tatagggctt ggactccctc gcttgcggat aggcgaatgc ccttccgctt tttttatgtt
     1981 tattggcttg gtgtagggca caagcaaaaa cattatggca atggaaaaga cacagcgaaa
     2041 acagtcccta aacttcttgt cagacactcc taagctgttt gacctcaaag aactattaag
     2101 ctcgcttaag atcacacccg gacaaatgga gaacatgaag gggttgatat acatcgaagg
     2161 gctgacagaa aaacaagtac gccagaaatt aagccattca aaaatcatca gaacaaatag
     2221 attcggggtt aattcctgcc tgtattatat cctctttgag gatgtaaagg ggagatattt
     2281 gtgcctgttt aacggtgaca gggaatgttt cagagcgttg tttttcagta aggtattact
     2341 aaattaaccc cctataagat agaattgtaa agttcttttc atccgcaaaa tggaatgaat
     2401 agaacgtaat atttatattg ttaaaatcta ttaacggggg ggtaaattct aatttgtatt
     2461 tctacacgct ttgaataata aaatatatac atatttttga accattaaaa tataaacttt
     2521 atcatggaaa aagcaaatat aggatttaaa gccgggatca tcttagaaaa attaggcgaa
     2581 accggactta taaccattgc cgagcttgcg agaaggctaa attttgcggc tgatgaaacc
     2641 gcactcgccg ccgggtggct cgcacgtgag aataagatat acatcgaacg gcgaaacggt
     2701 ttgctgtata tcaggaaaga gtgatacaac gtatcaaaag gataaacgat aaacgctgga
     2761 atgtttgcag tgcgtaacac tattgatgta aaagtgcttt tcattatcca tttgaaacca
     2821 tttataaact tataaaaaat acaggtatgg ataagcacat catcagagag aacgcaggga
     2881 aactctggcg cttattaagc acagacgtac accggaaatg gaatttgcag gaagtaaagg
     2941 aattgacagg gttcaatgat ctggaagtcg cctgtgcgat aggctggctc gctcgtgagg
     3001 acaagattca attcgagctt gaacaccacg acactaagga taagaacgca ttactatacc
     3061 ttatgttgaa cgtctatttc taaacacctc cctttgaatt gtaaaagcct ccggcggggt
     3121 tttcgccggg ggggcttctt gcatttgcat atataacctc aaaggcaagt caaatcggac
     3181 ggggtggcgg tcggacggct gaaaagtcga aaacgtaaca caggcgtgtt actacttgtc
     3241 tttgacaagg cattttttag gggtgctggc atacaatccg aaaggggcgg gaacggctgt
     3301 tcccgtttgt tctccctgca acctacagga tattaacctt aaatcattaa gtgataaaca
     3361 ctatatttaa taaccccttt tagccaacaa attgttatct ttgcgtaggg taaacaaaaa
     3421 ataagcttat gaaaactgct atggaaaaaa aagaaaccgg cttaatgaga cagatcggtt
     3481 atctctcttt tgtcgttgtt ttgttgtatg ggcttacgct atttgcggtt tcttgtgaca
     3541 atgatgaaga tcctcctctt cctcccgcag tggaaaaagt agtgctggca tttggtgtgg
     3601 atcagggtga aggtcagctt tcggctaaac cggagttgga aaatacggct gcggaaaaga
     3661 taggagaatt gatctctccg gctgaaatag cgaaaggcac aacggtgcat tttcgtgcta
     3721 cccattccaa atcatggaca atcatgtatt ggaaagtcaa tggcgtcatt attcgttctg
     3781 tggagccgga gcagactttc gtcatcgatg agcataagga ggttcgggtt gctttcaagc
     3841 cttacaagtc accggaaccg gaggggctgt aaaacaaact gtgtcagcaa agcaataaaa
     3901 tattaatttt gctgacacag tttaattgaa ttctcttttt gctgttcatt tgctttattt
     3961 caggacttcg cccttctcgt tgaagaacac ttcgctgtcc gtgccttctg catcggtaaa
     4021 tgtcaccttg taggtctttg tgccttcctc gctttcctca actgcggctt ccttgattgt
     4081 caggtctgcg aaatccttct tgatcgtgtc ctgaaccgcc tgcggcagat cctttacctc
     4141 gattggctta taatcgttca ccattaccac aatctcaaca tccgatacca tgttgccggc
     4201 gaatgccact gaactaccca atcccataac aagtgctact gcaaataata cctttttcat
     4261 aatccaatct atttaaatat ttaacatatg gtttctgaaa gacaaagaac aaacattgtg
     4321 ccaaacaaac aaacaagtca tatatacctg ataatcaaaa gcatacaaaa aacacatata
     4381 tttattgaag aactgttatc tgtgtatcat ttccacattt tgtggaaacg atacacagac
     4441 tttctataat tgcctccggc ggggttttcg ccggggggct tcttgcattt gcatatctat
     4501 caaaaagaaa gcaatatcgg agcgttacac cataaaaaca gctcgccggc aatgacaaaa
     4561 tgatattgcg tgtccggctg atcggggcgg gtctcccacc atgcggacaa atcccggtaa
     4621 cagaatgaca aaacggacac cccgccaaag aacaacattt tttttctttt ttcctaaaca
     4681 cctgccagcg aagcacagag tatttgtcaa gggcgaaggg taaaaaatac cgcacgagcg
     4741 aagcgagcac aggagatttt ttacccggag agtgaaacgc ccctttacaa atgcggcgtg
     4801 cgtagctacc ttcgtgccgg aaaatagaaa aaatcacgct acgactgccc caaaacgctt
     4861 tttatatctt cccttacctg tttgtaatag tccatgacat cagaacccaa aacaaacctt
     4921 tccggctgtt ctgttaccag ttgccggcaa cggctatcca cttgtttaaa atgaccgtga
     4981 aaaacaggtg ttgcgctctc caccgtgcgc ccgatgaatt cccctacctg taattccatg
     5041 aatacggacg ggcggtaaat gtcccgttcc tgcactccct cgctctgccc tttcgacttg
     5101 aacggcgata tagcaccctt gttctccgac cgtgtcgtaa atacggtgtc cgtcttgccg
     5161 aactgacgac tgaacacctc tgcggtcttg ctcgatgcga cacgcccgta aaaatggctg
     5221 ttgcaggctg cgaacaaaac atcggctttc ttatccccgt acccgtccgc aagctgcgca
     5281 agatcctgac acatcaaaac agtcgctatc ttgttactcc gtccggtgtt cggtatggtc
     5341 tccaaatgag gtataaagat tgtcggggct tcgtccagta acacgaaaga gggcgccttg
     5401 cccggttggt tcatcagctt tgtagcgacc gttataacca agctgcaaag cggtgacaat
     5461 gtttgtaccg ttgtcgggtt ggtggcaacc gtcaacaccg ccggggcttc cggatcgttc
     5521 agatcgagcg ggcaaccgtc acgggaaaac acccacatca tttcgggcgt gttgatctgc
     5581 gcaacggctc cttgtaacgt accaagtacc ccggaaactt ggttatccgc tcccctctgc
     5641 aacgcatcgt aaacgctgcg taccatttgc tccgtaatga tgttcttttg cagcgtgttg
     5701 agtaaggcag tgccattgct gccgaccatt gccagcacat ggggtatgtc gcatatctcc
     5761 gggtgttcct cccggagata ccagatacag gcggtcagca cgtcggttgc ggatctgctc
     5821 caaaagtccg ggttctttat gctctccttt gtcaagttgg caacgataga aacggcgtac
     5881 tcacgtgcgt agctcgtatt aggcacatat tccggcttta tgggattcac gaagtccgtc
     5941 cggtatggat cactgaaatt aagacaaaag tgtcttaaat cgctccctgt ggcgtttata
     6001 aaggctgtta tatcattgtc aagagccgga aacttgaaat cgtacacgat cccggcaaat
     6061 cccttgcgga tgaactcaag gagcaagggc actgcgacac tctcggactt tccggaacct
     6121 gccgcacccg ctataaagat gccccggaaa gggtttccca cgattaccgc cccttcatgg
     6181 gtgggaatgg ttaccgccca tgaaggggcg gctgcggtgt ccggctgttt tttccggaag
     6241 taacctttcg ggacgatgcg ccaaaggaca agacagcaca acagcacgaa gaacaccacc
     6301 aaccaaaggt aagcgaaccc ggcaatgtct ccagctttca tcctgcccca aatatccccg
     6361 aagtggagat cgagcacgga aatgaccttt accatgaaca cagcccacac caccagcaag
     6421 gcggtaaggc aacctttcct tttctttttc gccagctctc cctttttcat aagcccctgc
     6481 cccctattcc tttaacgatc ttctttacga ccttgttcgt gatctccttc tccgcttccc
     6541 gcctcgcttg atactggacg gctctcgatg cctgtttctt tatctctgcg ggcgtgccgt
     6601 tcttcatggc gttcagatac tcgaaacttt cctttacgtc ccggcggtag ttcgtccgga
     6661 gatcaaaccc ggtctcgcac ttccggaaaa actcggtacg gtcaaaaccg cccttaaccg
     6721 cccctttccc cgtgttccgg tggttggtct tggggcttaa cttgatcgta ttgctgcggt
     6781 ctttccggct tactatgatg tgggcgtgca tatccgttac ccctgccttg tcccgactaa
     6841 agtggatctt tgcgtaatac tcgatattgt ccgcttgcaa ccccttgttg aagttctccg
     6901 cataagccgg gataacatct ttccggatat actgtttcat ggcttgcgcc tgctgttcct
     6961 gcgtggctcc cattgctgcg agttccttct ctgacgggct gacggtgata acaaaaaact
     7021 ttgcgtccgt cttgtggagc tgcgcccggt tatgatcgat cttatacgtg acctctttgg
     7081 ggctgacgaa atcccggtac tggttgaaga acggctctac ctgttgtccc tgccggatgc
     7141 gctcaagatc ttcatgctgc aagtaagtta ctaccccggt acaactcccg gtattggcat
     7201 acgtgccgcc cccgccccct tgtatcttca cgttcatagg ctcttatccc tgctccttgt
     7261 acagtgtcgc aatgtcgttg aaaaaaccgc ttttctcctt tgcgtcaacg agcttgccaa
     7321 gatacatgaa cgctttccgt tcctgctcgg tggctctgcc caaaagctgg agtatgtcgt
     7381 ttcgcaagtt cctcgcatcg tagttctgcc gttgctgctg gtcggaaacg gctttctgca
     7441 agtcccgcac ctgtccggcg gttgccagct tgtcgagcac actctcgata cgggcttcgg
     7501 tggtgctgat cgctgcgcaa gccgggcgca agatgttctg ttcctgcttc cgaatgaacg
     7561 ctatcacttg atcgcaccgc ttgataagtt tctgcatctc ctttgccggg ctttcatgtt
     7621 gcaccgggtt aatgccgtat ttctcgaaat actccaacgc acaggaaagg aactctttct
     7681 ttgaaacatc gttagacttt gccagcctgt caaggataac ggctgtcttt ctatctattg
     7741 ttacggttgt ctgttcattc atacttttat acttgcatag gttattgcat acataaattt
     7801 cgcctacaat atactaatat ccagcgaatt acaaaaacat tgcatacatt ttgtatgcaa
     7861 gccgcatacg taacaggtaa taaataattg aaaatcagat atataaaaat tcagcattgg
     7921 acttagtcca atgactgctt gctcacttcg ttcactcact tcgttcacag gtaaaacccg
     7981 ccgataatag ctaaaataat tgcaatctat aaaactatca ttctataata cagaagatta
     8041 gtttttatat caattagtga atcattggct agtctattat taaacggctg attttcagcc
     8101 ataatcccgt agggcaagag gaaaaaacgg tacgtagtgc cgtttcctct tgctgttctt
     8161 tcggctgctt tgcatccggc gaacaccctc ccctgcggta cggcaaaagg gtacttttgc
     8221 atcgtacaag ggggacgggg agagccgggc aaagcagccg aaagaaccta ttccacaaac
     8281 aaaaaacaca cgccaaaacg acacaacagg ctgtataaaa taaatagaaa ttgtttcgct
     8341 atatggaaac aaaagattat ctttgcacca taaaaacaac aacttatgga agaaaaggct
     8401 aaattcaaaa tcatctactc aaaagatgca gatgattttc tgaacaactt accaacaaag
     8461 attaaagaca aaattatcta taatattgcc aaagccaagt ttgtgataga tccggaactc
     8521 tttaaaaagt tagacgatac ggatatatgg gagttcagaa cattctataa caaagtaaaa
     8581 tacaggctgc tggctttttg ggataaagat aacggaacaa atacccttgt tattgcaaca
     8641 catggattta taaagaaaac ccaaaaaaca ccacccaagg aaatagcgaa agccgaagat
     8701 attagaaaag catattttaa ttcaaaaaaa taaagcgtat ggaaacaatg aaattttata
     8761 cagaagaaga aatattagat aagcacatag ggaaaaaagg cactcccaaa agagatcagt
     8821 ttgaagctga tctaaactct ttcttaattg gtgaagccat taaacaggct cgccagtcga
     8881 agaacctaac ccaagaggaa ttaggcaatc tgataggagt gcagcgtgct caaatctccc
     8941 gaattgaaaa cggtaagaat ttaacctttt ccaccattgc aagagttttt aaggctatgg
     9001 gaataagtgc taaacttgaa atcggaagca tgggtaaagt tgcgttatgg taactaacta
     9061 tacaacaaaa tgttatatca aatccgggag cgaaaacgca tcccggaagc ctctcttttt
     9121 atacacaaaa attcatattt acacaaaatt taacacgcct aaaaattctc cttccttccg
     9181 ctgcgcttgc tttacctttt ccgtgatttt cttctcactt tctctctcgc ggtccgtccg
     9241 gatggcaagc gaagcgccta taaaatctca aaaatcgaca cgtgcgtatt actggtaatt
     9301 ttgagttact tttgagtacc tttttcggca tgcttcaaac cgtttattca tgcgggtttc
     9361 aagatatcta ttacaacctt tttacgagct attgcaactt ttttacgagc tattgcaact
     9421 tttttacgca aaaaagggga tattaaaaca attatattgc aactttattg tataattggt
     9481 ttcaataaca tatatttgca acgtaaaaca atacagtt
//
