LOCUS       NZ_CP072221             9081 bp    DNA     circular CON 07-APR-2024
DEFINITION  Bacteroides uniformis strain CL11T00C07 plasmid pBuniCL11T00-1,
            complete sequence.
ACCESSION   NZ_CP072221
VERSION     NZ_CP072221.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16451196
            Assembly: GCF_018289175.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides uniformis
  ORGANISM  Bacteroides uniformis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Garcia-Bayona,L., Coyne,M.J. and Comstock,L.E.
  TITLE     Mobile Type VI secretion system loci of the gut Bacteroidales
            display extensive intra-ecosystem transfer, multi-species spread and
            geographical clustering
  JOURNAL   PLoS Genet 17 (4), e1009541 (2021)
   PUBMED   33901198
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Coyne,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) Department of Medicine/Division of
            Infectious Diseases, Brigham & Women's Hospital, 181 Longwood
            Avenue, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-OCT-2020
            Assembly Method                   :: FALCON-Unzip v. 1.2.0
            Assembly Name                     :: BuniCL11T00C07_pbv2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 271x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018289175.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 07:52:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,676
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,499
            CDSs (with protein)               :: 4,499
            Genes (RNA)                       :: 93
            rRNAs                             :: 5, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 29 of 84
            Pseudo Genes (incomplete)         :: 49 of 84
            Pseudo Genes (internal stop)      :: 22 of 84
            Pseudo Genes (multiple problems)  :: 14 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072221.1.
            Sequencing by the Institute for Genome Sciences, Genomics Resource
            Center, School of Medicine, University of Maryland, 801 W.
            Baltimore Street, Baltimore, MD 21201, USA. Gene calling method:
            Prodigal version 2.6.3. Annotated using a custom modification of
            Prokka version 1.14.6.
            Bacteria available from Dr. Laurie Comstock, Brigham & Women's
            Hospital, 181 Longwood Avenue, Boston, MA 02115 USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Bacteroides uniformis"
                     /mol_type="genomic DNA"
                     /strain="CL11T00C07"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:820"
                     /plasmid="pBuniCL11T00-1"
                     /geo_loc_name="USA: Boston"
                     /lat_lon="42.3361 N 71.1075 W"
                     /collection_date="2009-05"
     gene            1..978
                     /locus_tag="INE90_RS22550"
                     /old_locus_tag="INE90_04537"
     CDS             1..978
                     /locus_tag="INE90_RS22550"
                     /old_locus_tag="INE90_04537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669318.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_008669318.1"
                     /translation="MEKKDKKQRDIVLSQDNALTTARYKFDLIEKRALYSIIRNIRAQY
                     IENENGRRDLFDDLIITIHEEELKKCGDNTKMQNIYDALKRLRNRDIEINNEKMWLSIG
                     FVNYVKHIKNKSYFEVQVSKEVLPYYVELAQNFTSYSMTVAIALKSTFSQRFYELCCQY
                     RNAGFFFYTVEKLREMFMLEKKYKRGCDFKRRVLDDPQKELRELYNAGQCDLYFEFEPK
                     SYKGKEVTEYKFYVYTRQTEQQKLLEYESAKQAIIYITTTISRFSKSDKGYVKRVVNAV
                     QLHPEIAVQVAQKIQKKFEQYIDKPAKQIGAIIRVCLMEDFGIE"
                     /name="replication initiation protein"
     gene            992..1183
                     /locus_tag="INE90_RS22555"
                     /old_locus_tag="INE90_04538"
     CDS             992..1183
                     /locus_tag="INE90_RS22555"
                     /old_locus_tag="INE90_04538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021409502.1"
                     /translation="MKTGDKVIVPAEINGYGRDLRAIVTEIEKFAGAIFVTVIFTEPCP
                     EACGRGGGVYHDFQLIAR"
                     /name="hypothetical protein"
     gene            1286..1807
                     /locus_tag="INE90_RS22560"
                     /old_locus_tag="INE90_04539"
     CDS             1286..1807
                     /locus_tag="INE90_RS22560"
                     /old_locus_tag="INE90_04539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008669313.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAKKLDEYLNGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVAGRLEQSAKIMERHNEGTEAVHLLLQAVREQTAK
                     QLPAPELIAHAAEEKAKAEAKSEEQEREIARLRSENSALLEYKEKAYRELCRIRDEQKT
                     IGKIKVNIEL"
                     /name="hypothetical protein"
     gene            1837..2379
                     /locus_tag="INE90_RS22565"
     CDS             1837..2379
                     /locus_tag="INE90_RS22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="WP_080974082.1"
                     /translation="MKKFYLFILLPDYAAHEAVYFSQAITADDFAMKKNPKYVNKVVAP
                     TLEPISSIGGFRTLPDYSFDTMPDDYVALVLIGGFGWNTPVAEKVMPIIKSAIEQEKVI
                     GAICNAASFMAKCGIDRETTSKTLQKSSVIQKISVTLSSVIIAIIVCKLLIFNTLRLQQ
                     FFANHHFSGTYISSNSR"
                     /name="DJ-1/PfpI family protein"
     gene            2444..3499
                     /locus_tag="INE90_RS22570"
                     /old_locus_tag="INE90_04541"
     CDS             2444..3499
                     /locus_tag="INE90_RS22570"
                     /old_locus_tag="INE90_04541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781140.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_215424929.1"
                     /translation="MIVMAMLAFFVFVSRYYVTCRNRQFEQSRWMIVVALLLFVVHYMC
                     QMRLGWRQQGNDVGVLFNLLFYSPSAILLSWSQLNILRAGHRRWSFMRYGVVGYALMVL
                     CIVAGVISNGSLHIGPMLYVADAIHFFTLLYYTWAPLRELGNVQRRLDSEHGNPADAYL
                     RTMRFGFFVVCAFAVISPLYILSRPLLFVFGPLGLISLSLFIVSFTALGFSMSEGVTEI
                     VTETNDEIAAAKVFREIGPERIAEIDMAVSKWRSEGGFRDSNLTLSSFARRIMVNRTDV
                     ASYLTSIYGKNFRTWLSGIRVEEAKALMAAHPEYSNEVVSMECGFSSRVYFQRLFKERT
                     GLTPAEWRRQR"
                     /name="helix-turn-helix domain-containing protein"
     gene            3719..5374
                     /locus_tag="INE90_RS22575"
                     /old_locus_tag="INE90_04542"
     CDS             3719..5374
                     /locus_tag="INE90_RS22575"
                     /old_locus_tag="INE90_04542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008669305.1"
                     /translation="MQKTYLYLMVCVIGAMSLASCSEDSISPDAGGDGDGDSTTEVPLA
                     QVAKPKPNPWLAQEEYSITHFNSAQTDAFSSAVKDGTFNIDLSKCQMTWSGPVNLMTLA
                     STSPNYMWGMSSDRVSYLDISDGKLERVAEAGLPGITMKTQEQLTRIIADHATYDELSK
                     TVTDILGPVPQMSMANGNYVLCDKDNYAYTNAGQVMARYRLANPNNPKDGIMLDHQIRL
                     TNFTFGSFTLVGATMTYDGHLVVAAQNGLLVLNRALTTIEDSYPLPSDQILTNSICIDE
                     NGGVYVASNSRTPGGKGLMQKLICKDGKISTSQADGAWQAYYDGGPQAPCIKLGHGTGS
                     TPTLMGFGQDKDKLVVITDGSKRMKLVAFWRDEIPSDAQQVAGYDKRIAGVHEVTCGLG
                     TSTEWIQSEQSVVVGGYDAFVVNNINVTNQEINDKIIGVIAIGPIVKGPQGAECVRWNT
                     KEKKWESKWTRSDVSSVSMIPAVSIKSEMVFVCGWNDASGWEVTGLDWKSGATRHRSIL
                     GKNNRANGAYAIIQYLANGDLLFNSVAGPIRVKY"
                     /name="hypothetical protein"
     gene            complement(5929..6807)
                     /gene="btgB"
                     /locus_tag="INE90_RS22580"
                     /old_locus_tag="INE90_04543"
     CDS             complement(5929..6807)
                     /gene="btgB"
                     /locus_tag="INE90_RS22580"
                     /old_locus_tag="INE90_04543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgB"
                     /protein_id="WP_008669303.1"
                     /translation="MNVKIQGSGNGTYANTGSCVAVTNYLQHEDLERMKKGEEVQPFFN
                     QFRDYVSAREVTFKIDNNKAKLSRTDAKFYVITVSPSEKELRCMGRTPQECAEALRRYI
                     RQDVMRNYAEGFGKGLKSDDVEYYAKIHFNRDGESDSDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDRRMGFDREPEESFDYLNAVKNGSPAEIAR
                     QVERAERIRKEKWDKLKAELQSRQEPEKSKNQQVEKTPDIEQPIPKKKQQEEDLELLKK
                     PKRSRGFGMGM"
                     /name="btgB"
     gene            complement(6804..7409)
                     /gene="btgA"
                     /locus_tag="INE90_RS22585"
                     /old_locus_tag="INE90_04544"
     CDS             complement(6804..7409)
                     /gene="btgA"
                     /locus_tag="INE90_RS22585"
                     /old_locus_tag="INE90_04544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgA"
                     /protein_id="WP_008669301.1"
                     /translation="MDKETTTSVTIDRKTFARLDRLAKSNNVSKKEFLSCALEYFEKYG
                     INPVEHESPAKEMQKLIKRCDQVIAFIRKQEQDFLRPACEAMGSTSMRVTMSMDSILTE
                     KKFSQYQKDNDLFMRDLASLAGIREQALDRAEKAVGQSRDMLLKNQQAIYARLDAVTQR
                     QEKIFSYIASYIDAKGKAGLFDDIKALYKNEKNKREKI"
                     /name="btgA"
     gene            7939..8286
                     /locus_tag="INE90_RS22590"
                     /old_locus_tag="INE90_04545"
     CDS             7939..8286
                     /locus_tag="INE90_RS22590"
                     /old_locus_tag="INE90_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_008669299.1"
                     /translation="MEAKSRFKVKLSNEAQEFLDGLPNKVREKIISNIRKSTFVLDPDL
                     FKKLDDTDIWEFRTFYNKTKYRLLAFWDKDDKINTLVIATHGFIKKTQKTPPKEIAKAE
                     EIRKDYFNSKR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8292..8606
                     /locus_tag="INE90_RS22595"
                     /old_locus_tag="INE90_04546"
     CDS             8292..8606
                     /locus_tag="INE90_RS22595"
                     /old_locus_tag="INE90_04546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_007851388.1"
                     /translation="MEAKKFYTLEEIEDKYIGEKGTPKRDAYEEELNSFLIGEAIKQAR
                     QSKNLTQEELGNLIGVQRAQISRIENGKNLTFSTIARVFKAMGISAKLEIGSMGKVALW
                     "
                     /name="helix-turn-helix transcriptional regulator"
     Typing          2..978
                     /name="rep_cluster_1097"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.898"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5933..6807)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggagaaga aagacaagaa acagagggat atagtcctgt cgcaagacaa tgcgctgact
       61 accgcacgct acaagttcga ccttatcgaa aaacgtgccc tatactccat tatacgaaat
      121 atccgggcgc aatacatcga gaacgagaac ggcagacggg acttattcga tgatctgatt
      181 atcaccatac acgaggaaga actaaagaaa tgcggtgaca ataccaagat gcaaaacatc
      241 tacgatgcgc taaaacggct tagaaatcgc gatatagaga ttaacaacga aaaaatgtgg
      301 ctaagtatcg gttttgtcaa ttacgtcaag catatcaaaa ataaatcgta ttttgaggtg
      361 caagtttcca aagaggtact accctattac gtggaactcg cccaaaactt cacgagttac
      421 tccatgacgg tagcaatcgc actcaaaagc acattctcgc aaaggtttta cgaactgtgc
      481 tgccaatacc gaaacgccgg gttctttttc tacacagtcg aaaagcttcg ggaaatgttc
      541 atgttggaaa agaaatacaa acggggatgc gatttcaaaa ggcgtgtact tgacgatccg
      601 caaaaggaac tccgggagtt gtacaatgcc ggacaatgcg atttatactt cgagttcgag
      661 ccaaagagct acaaaggcaa ggaagttacc gaatacaaat tctacgttta cacaaggcaa
      721 accgaacaac aaaagctatt ggaatacgaa agcgcaaaac aggcgatcat ctacataaca
      781 accactattt cgaggttctc taaaagtgac aaaggatatg ttaagcgtgt agtgaatgcc
      841 gtacagttac acccggaaat agccgtacag gtggcgcaaa agatacaaaa gaagtttgag
      901 caatatatag acaagccagc caaacagata ggggctatca tccgggtttg cttaatggaa
      961 gattttggta tagaataaaa aacatacaag tatgaaaaca ggtgataaag ttatcgtacc
     1021 agccgaaata aacggctatg gcagagattt acgggcaata gttacagaga tagagaagtt
     1081 cgccggggca atattcgtaa cagtgatatt caccgaacca tgtccggaag cctgcggaag
     1141 aggggggggt gtttaccatg acttccagct aatagcaagg tgatttattt tgctataatc
     1201 gcaataaatt gcaataatat ctgtgataaa ttgctataat aatacttata actacctttg
     1261 caacagaata tagcatttta cagcaatgga gaaaggacag gaaagcaagc aaaagaggaa
     1321 aacaccgctt accaccatag cgataagcca agagtacgcc aaaaaattag acgagtattt
     1381 aaacggtacg ggtataactc gcaaagaatt tgtcgagttg tccgttgatt atttccaaag
     1441 aaccgggttc gacctccggg gtgaagcttt tgacctctcc ccattagaaa aggttgccgg
     1501 gcggctggaa caatccgcaa agatcatgga acgacacaac gaggggacgg aagcggtgca
     1561 cctgcttctc caagctgtcc gggaacagac cgccaagcaa ctcccagcgc cggaactgat
     1621 cgcccatgct gccgaagaaa aagcaaaggc agaagccaaa agcgaagaac aagaaaggga
     1681 gattgcccgg ctacgctccg aaaactccgc tttgctggag tacaaagaaa aggcataccg
     1741 ggaactatgc cggatacgtg atgaacaaaa aaccataggg aagataaaag taaatataga
     1801 gttataaaag aacatttata aataaaaaaa tcatgaataa agaaatttta tttatttatc
     1861 cttttgccgg attatgcagc acatgaagct gtgtactttt cacaggctat tacagctgat
     1921 gattttgcca tgaaaaaaaa tccaaaatat gtcaataagg ttgtcgcacc caccttagaa
     1981 ccaataagtt cgattggtgg attccgcaca ttacccgatt attcgtttga cacaatgcct
     2041 gatgattatg tagcattggt attaattggc ggttttggtt ggaatacacc tgttgcagaa
     2101 aaggttatgc caattattaa aagtgctatt gaacaagaaa aggttatagg tgcgatttgt
     2161 aatgcagctt cattcatggc taagtgtggt attgaccgag aaacaacatc aaaaactttg
     2221 cagaagtcat ccgtaataca gaaaatctca gtaactttgt cctcggtaat catagcaatt
     2281 attgtctgta aacttttgat atttaacact ctaagattac aacaattttt cgctaatcac
     2341 catttttcag gcacttatat ttcgtcgaac tcacgttaaa ttatttaata tacatacaat
     2401 cttggatact tgcttagacc ttattgctct tcaatatact tgcatgattg tgatggccat
     2461 gcttgcattt tttgtgtttg tgtcgcgcta ctatgtaact tgccgtaaca gacagtttga
     2521 gcagtcgcgt tggatgattg ttgtggcatt actattgttt gttgtacact acatgtgtca
     2581 gatgcggttg ggttggcgac aacaggggaa cgatgtgggt gttctgttta atctgctgtt
     2641 ttattcgcct tcggctattt tgttgtcatg gtcgcagctt aatattctgc gcgctgggca
     2701 tagacgttgg agttttatgc gctatggcgt tgttggatat gctcttatgg tattgtgcat
     2761 agtcgcaggt gttatttcaa atggcagtct gcatattggt cctatgctat atgttgctga
     2821 tgctattcat ttctttactc tattgtatta tacatgggca ccgctgaggg aacttggtaa
     2881 tgttcaacgt cggctcgaca gtgaacatgg taatcctgca gatgcctacc tgcgcactat
     2941 gcgctttggt ttttttgtgg tgtgtgcttt tgctgtaatt tcgcctcttt atatattgtc
     3001 gcggccgctg ctctttgtct ttggaccttt gggattgatt tcgctttcgc tgtttattgt
     3061 tagttttacg gcgcttggat ttagtatgtc cgagggagta actgagattg ttaccgagac
     3121 taacgacgag atagccgctg ctaaagtctt tagggagatt gggcctgagc gtattgccga
     3181 aatagatatg gcggtaagca aatggcgaag cgagggcggt tttagagata gcaacttgac
     3241 attatcctcg tttgctcgtc gcataatggt taatcgtacc gacgttgctt cgtatctgac
     3301 ttccatatat ggtaagaatt tccgtacatg gctttcgggt attcgcgtgg aggaggcaaa
     3361 ggcactcatg gctgcacacc ccgaatatag taacgaggtt gtttctatgg agtgcggttt
     3421 ctcgtcgcgt gtgtattttc agcgtctgtt caaggaaagg acgggcttga ctcccgccga
     3481 gtggcgccgt caacggtaat atactggtta tgtgtgcgtt tgctactgaa tgtaaggtgt
     3541 aacttttgta cgaaggttac ataataaggc aaagtgtaac ctttcttact gtgacgtatc
     3601 ctttctataa gagtgacaca caagtgatgt ctttgaggtt tttgatggtt gatgctcctg
     3661 tatctttgca tgaggaaatt caaactattc aaatataaat tctcaaaaac aattcactat
     3721 gcaaaaaacc tatctttatt taatggtgtg tgtcataggg gctatgtctt tagcttcgtg
     3781 ttccgaagat tctatttctc ctgatgctgg tggcgatggc gatggcgaca gcactaccga
     3841 agtgcctttg gcacaagttg caaaacccaa acctaaccct tggcttgcac aggaagagta
     3901 tagtattacg cacttcaatt cggctcagac tgatgctttc tcgtctgctg tcaaggatgg
     3961 aactttcaat atcgatcttt ccaagtgtca gatgacatgg agcggtcctg ttaatttgat
     4021 gactcttgct tcgacttcgc ccaattatat gtggggtatg agtagcgacc gcgtgtcgta
     4081 tcttgacata agcgacggca aacttgaaag ggttgccgag gctggcttgc cagggattac
     4141 aatgaagacc caggagcaac tgacacgtat aattgccgat catgctactt acgacgaact
     4201 ttccaagacg gttaccgata tattgggtcc ggtgccacaa atgtctatgg ccaacggtaa
     4261 ttatgtcttg tgcgacaagg ataactatgc ttacaccaat gcaggtcagg taatggcacg
     4321 ctatcgcctt gccaatccta ataatcccaa ggatggtatc atgttggatc atcagataag
     4381 acttacgaat tttaccttcg gctcttttac actcgtaggc gctactatga cctatgacgg
     4441 acaccttgtt gtggctgctc agaacggact tcttgtgctg aatcgtgctc ttactacaat
     4501 cgaagattca taccctcttc catcagacca gattctcacc aactctatct gcatcgacga
     4561 gaatggcggt gtttacgtag caagtaacag ccgcacacca ggcggaaaag gccttatgca
     4621 gaaactcatc tgcaaggacg gcaaaatctc cacctcccag gctgatggtg catggcaagc
     4681 ctattacgac ggtggtccgc aagcaccatg tatcaaactc ggacatggca ctggttcaac
     4741 gcctacactt atgggctttg gccaagacaa ggacaagctg gtagttatta ccgatggctc
     4801 taagcgtatg aaactggtag ctttctggcg tgacgaaatc ccctcagacg cacagcaagt
     4861 ggcaggatac gacaagcgta tagccggagt gcacgaggtt acatgtggtc ttggaacatc
     4921 aacagagtgg atacagtctg agcagtctgt tgtagtaggc ggctatgacg cctttgttgt
     4981 aaacaatatt aatgttacca atcaggaaat aaacgacaag attattggtg taatcgctat
     5041 tggtcctatt gtcaaaggcc ctcaaggtgc cgagtgtgta cgttggaata caaaggagaa
     5101 aaagtgggag agtaagtgga cgcgcagcga tgtaagttct gtaagtatga ttcctgccgt
     5161 aagtatcaag tcagagatgg tatttgtatg tggctggaac gatgcttcgg gctgggaagt
     5221 tacaggactt gactggaaga gtggtgccac acgtcaccgc agcatattgg gcaagaacaa
     5281 ccgagccaat ggtgcctacg ccatcattca gtatcttgct aacggcgacc ttttattcaa
     5341 ctctgttgcc ggaccaatcc gcgtaaaata ttgagataat ttaatcatca tataacagtt
     5401 atgtcaatac ttcactaaaa aattaccgaa gtattgtaat gacacacccg aacatattga
     5461 aatgttctat cagttccata aacaaggctt ctgttcttta ttcctagata caggtgataa
     5521 ataacagata agaaacagat tataacaagc ctccggcggg gttttcgctg ggggcttttg
     5581 acctttgcac atataaaaag caaaaagaaa gcgatatcgg agcgtatacc ataaaacaga
     5641 ccaccgacta ttacaaaacg atattacgtg cctaactgat cggggcagtt cctccaccat
     5701 gcggacaaat cccggtaaca aaatgacaaa acggacactc accaaagaac aacatttttt
     5761 tcttttttcc taaacacctt ccagcgaagc acagtgtatt tgtcaagggc gaagggtaaa
     5821 aaataccgca cgagcgaagc gagcacagga gattttttac ccggagagtg aaacgtccct
     5881 ttacaaatgc ggcgtgcgga gttacctttg tgccggaaaa tagaaaaatc acatgcccat
     5941 tccgaaacct ctgctccttt ttggcttctt caggagttcc agatcttcct cctgctgctt
     6001 ctttttggga ataggctgct cgatgtccgg cgttttctcc acctgttgat ttttcgactt
     6061 ctccggctcc tgccgggatt gaagctctgc tttgagtttg tcccatttct cctttctgat
     6121 gcgttccgca cgttccacct gccgggctat ttccgccgga cttccgttct tgacggcatt
     6181 aagatagtcg aagctttcct ccggctcccg gtcaaacccc atgcgccgat caaaagagct
     6241 ttcacacttc cggaaaaaat ccgtccggtc aaaacctccc ttcacgttgc cgcagttctt
     6301 cttcccggtg tggttcgttt tagggcttaa cttcctggtg ttgctccggt ctttccggct
     6361 gacgatgata tgtgcgtgca tatcgctgtc actttcaccg tcccggttga aatggatctt
     6421 tgcataatat tccacatcat cgcttttcaa gccttttccg aagccctccg catagtttct
     6481 catcacatcc tgccgtatgt accgccgtaa ggcttccgca cactcctgcg gagttctgcc
     6541 catgcagcgc agctccttct ctgaagggct gacagtgata acatagaact ttgcgtcagt
     6601 ccggcttaac ttagccttgt tgttgtctat cttgaatgtg acctcccttg cgctcacgta
     6661 gtcccggaac tggttgaaga acggctgcac ctcctcccct ttcttcatcc tctccaaatc
     6721 ctcatgctgg agatagttcg tcaccgcaac acaactgcct gtattggcat atgtgccgtt
     6781 accgcttccc tgtatcttca cgttcatatc ttttcccttt tgttcttctc gttcttgtac
     6841 aaggctttta tatcatcgaa aagcccggct tttcctttcg catcgatata gcttgcgata
     6901 taggagaaga tcttctcctg cctttgcgtc acagcgtcca gccttgcata aatggcttgt
     6961 tggttcttca gaagcatatc cctcgactga ccgactgcct tttccgcccg gtccaaagcc
     7021 tgctccctta tcccggcaag gcttgcaagg tcacgcatga ataggtcatt gtctttttgg
     7081 tattggctga actttttttc cgtaagtatc gagtccatgc tcatggtcac tctcatgctt
     7141 gtactgccca tggcttcaca agccggacgc aagaaatcct gctcctgctt tctgatgaac
     7201 gctatcacct gatcacagcg tttgataagt ttctgcattt cctttgccgg actctcatgc
     7261 tcaaccgggt tgatgccgta cttctcgaag tactccagcg cacaggaaag gaactctttc
     7321 tttgatacat tattggactt tgccagccta tcaagccggg caaacgtttt tcggtcaatg
     7381 gtgacagaag tggtggtttc cttatccata caattgcggt tttaattgct gtttatatta
     7441 ttaaatctct gatttccaaa ggtatgattt taaccacaat ttcaaactaa ttgcggttaa
     7501 aaaacttaaa cggctgatat tcagccgtaa ccccgtaggg caagaggaaa aacagggtaa
     7561 agcccggttt cctcttgcta ttctttcgac tgccgagcaa tcgacgaaca ccctcccctg
     7621 cggtacggcg aaagctcgta caagggggac ggggagaagt cggagcgagg cacaaagaaa
     7681 gaacctattc cacaaacaaa aaaacacatc aattgctatt ctttcgactg ccgagcaatc
     7741 gacgaacacc ctcccctgcg gtacggcgaa agctcgtaca agggggacgg ggagaagtcg
     7801 gagcgaggca caaagaaaga acctattcca caaacaaaaa aacacatcaa aacggcacaa
     7861 caggctgcat aaaatatcaa tattttgttt cgctatatgg aaacaaaata ttatctttgc
     7921 aacactaaaa aactttatat ggaagcaaaa tcaagattca aggtcaaatt atccaacgaa
     7981 gcccaagagt ttttagacgg attgcccaac aaggtacgtg agaagatcat tagcaatatt
     8041 cgtaaatcca cctttgtact tgatccagat ttattcaaga aattggatga tacggatatt
     8101 tgggaattca gaacgttcta taacaaaacc aagtacaggc ttcttgcttt ttgggataag
     8161 gacgataaga taaacactct ggtaatcgca acacatgggt ttatcaagaa aacccaaaag
     8221 acacctccca aagaaatagc caaagccgaa gagattagaa aagattattt taattcaaaa
     8281 agataaagcg tatggaagca aagaaatttt atacactcga agaaatcgag gataaataca
     8341 tcggagaaaa aggtactccc aaacgtgatg cctacgaaga agaacttaac tcttttttga
     8401 taggtgaagc cattaaacaa gctcgccaat caaagaactt aacccaagag gaattaggta
     8461 atctgatagg agtgcaacgg gctcaaatct cccgaattga aaacggtaag aatttaacct
     8521 tttccaccat tgcaagagtt tttaaggcta tggggataag tgctaaactt gaaatcggaa
     8581 gcatgggtaa agttgcgtta tggtaactaa ctatacaaca aaaagttaca ttaaatccgg
     8641 gatgcgtttt cgctcccgga aacttcttgt tttatacatt aaaagtcata tttatacaaa
     8701 atttaacacg ccaaaaaatc ttcctaatcc gttgcacttg gctttacttg tttccactct
     8761 ctcccattct ccggaaattc gtcgtatcaa aaaacacgct gaaaagtgga gcgaagcgac
     8821 ctctataaac caaaaaagaa acactccatt gctttacggt aatttttagt ttctttttag
     8881 ttacttttac ggcatagcat aacacattgt gctgtaatat cttacaaagg tcatctacga
     8941 caaatttccg agcatctacg acaaatttcc gagcatctac gacaaatttc cgaaaaaaac
     9001 ggttatctac gacaaatatt ctacgccata tatttgtttt tgtcgtagat aaatatttcc
     9061 tttgcgaaaa gcattaaagt c
//
