LOCUS       NZ_CP071969             1979 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Enterococcus faecium strain VB13828 plasmid p11VB13828, complete
            sequence.
ACCESSION   NZ_CP071969
VERSION     NZ_CP071969.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18353823
            Assembly: GCF_017603725.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Complete genome of vancomycin resistant E. faecium_VB13828
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: CANU v. v.1.7; UNICYCLER HYBRID v. v.0.4.6
            Assembly Name                     :: VB13828
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017603725.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:36:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,851
            CDSs (with protein)               :: 2,851
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 52 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 38 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071969.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB13828"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p11VB13828"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2020"
                     /collected_by="Christian medical college"
     gene            256..1272
                     /locus_tag="J4767_RS15415"
                     /old_locus_tag="J4767_15415"
     CDS             256..1272
                     /locus_tag="J4767_RS15415"
                     /old_locus_tag="J4767_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002326787.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDKV
                     HPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLF
                     DQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDI
                     LGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIV
                     PKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHS
                     EWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1472..>1654
                     /locus_tag="J4767_RS15420"
                     /old_locus_tag="J4767_15420"
                     /pseudo=""
     CDS             1472..>1654
                     /locus_tag="J4767_RS15420"
                     /old_locus_tag="J4767_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          506..1272
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaggtcttt ttattttttt aggaggagtg ctataatcaa agaaaacgag aaaaaagagc
       61 aaaataaaag ccgagcagtg cgctaacact ctcgactatt tacggaaact acttcaccta
      121 gctccgtaac tcaggttcat tatacgaata tttttaggtt tcttcaagct agagattttt
      181 ggatatgaat ttttagggtt acgtgaactc tggaaattct ttccaaaagt ctcttttttc
      241 gtctggagga cgaaaatggc aaatgaaaat cagttgagtg aaattcaatc aaaaattgaa
      301 ggtcgagggg cagaaaaaag aacaacgata aatcagctcg aaaaacttaa ccgtatttct
      361 aacaggcggt taagtgttga cgataaggta caccccctaa aattaagttg gtcaatcgat
      421 cgaattacaa tcgtcggaaa actgaatgat accatagctt attggacgca aaatggagtt
      481 gtgtttgtcg attttgagca attaatgcgt ttgaatgaaa atcaagggta tttgaagtcg
      541 gttggtacaa atggttggca attatttgac caatatggag aagatatcgc ctatatcgaa
      601 atgttaaaat ttcaccctga caaaggtcgt attgatttta atccaaataa aattggcgag
      661 tttttaaagg ggtcgatgaa acaattcatt catgatttgt ttctggatgc gcatttctca
      721 cgtgcagata ttgcttgcga tattctcggt gttcctgatg attttattac ccagtatcga
      781 atcgttgacc ctgtgtcttt taagccaatt tatggccgtt ctggcaaatt agaaacagcg
      841 tactggggta gtcgtgccag cgagcgacaa gtgcggatgt acaacaaaaa gcttgaacag
      901 gaacgaaaaa aacagattgt accaaaagaa atcgaaacat ggtggcgtat agaaatgcag
      961 ctaagacgtg gcaaagcaac agattggcat gcgatggttc gggaaagttt agatagcttt
     1021 gcaagtcctc attacttgcc tggagatgta aaacctgttg atagaattat gataaaaggg
     1081 ctgaatcaag atcattcaga gtgggcgtat attagcagaa atttgaaata tagattgcgc
     1141 aaattgttga aagaagaaag ccaaaacgat gagttgacga atcatttacg agagacgttt
     1201 aaggaatctg caaacgactt gaaaatagaa ttagacacgt ggcttttggg tttggacgtg
     1261 acagagaagt gataccgaat aagaaaagtc agatgagtga gagtaaattt acgtcaatgt
     1321 ttaatgacgt gagtttacga taagcgatta ttttgcgtga agcaaaataa agcgtaaaag
     1381 gagttgagag cgtgacacgc tttcggctcc ttttttcgtt gtcacttgtg acagcgaata
     1441 ccctcggaga gcatttggaa ttgtgacagc catgtcacaa ttaggaaaat gttactcttt
     1501 gtcaaagagt aacagaaagg tttacactct ttttattagc gacagcgcga aggaggggtt
     1561 caccttaggg tttagtattt cgttcaaaaa gggaacaaag aatacaagca tttctcataa
     1621 taatcgtgag ttaactgata gccaaaaaat caatggtttg tctggtagaa aataattagc
     1681 agatttacaa gtgggaaata aaaagctgaa agaagaaaat tgcatttcaa actacatttt
     1741 ctacaatagc tctaaaatgg catattttgc gttctgagcg gttttagcgt ttatttcgtt
     1801 tagttatcgg cacaatcgtt aaaacacgtt agaggagcga aaatggagcc tgtaaggcga
     1861 ctggtagcgg aacgatcgtg ttagattgtg aaaaccgata gctgaatgaa ataaacagcg
     1921 ggtgcgccct ttccatttcg gcaggaggag gctcaagggg ttcggggaat gaaattccc
//
