LOCUS       NZ_CP071968             2056 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterococcus faecium strain VB13828 plasmid p10VB13828, complete
            sequence.
ACCESSION   NZ_CP071968
VERSION     NZ_CP071968.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18353823
            Assembly: GCF_017603725.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Complete genome of vancomycin resistant E. faecium_VB13828
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: CANU v. v.1.7; UNICYCLER HYBRID v. v.0.4.6
            Assembly Name                     :: VB13828
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017603725.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:36:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,851
            CDSs (with protein)               :: 2,851
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 52 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 38 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071968.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB13828"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p10VB13828"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2020"
                     /collected_by="Christian medical college"
     gene            join(1992..2056,1..157)
                     /locus_tag="J4767_RS15395"
                     /old_locus_tag="J4767_15395"
     CDS             join(1992..2056,1..157)
                     /locus_tag="J4767_RS15395"
                     /old_locus_tag="J4767_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            158..415
                     /locus_tag="J4767_RS15400"
                     /old_locus_tag="J4767_15400"
     CDS             158..415
                     /locus_tag="J4767_RS15400"
                     /old_locus_tag="J4767_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(551..1153)
                     /locus_tag="J4767_RS15405"
                     /old_locus_tag="J4767_15405"
     CDS             complement(551..1153)
                     /locus_tag="J4767_RS15405"
                     /old_locus_tag="J4767_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1220..1375)
                     /locus_tag="J4767_RS15410"
                     /old_locus_tag="J4767_15410"
     CDS             complement(1220..1375)
                     /locus_tag="J4767_RS15410"
                     /old_locus_tag="J4767_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     Typing          318..493
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(624..1153)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agaacttgat tacaaccaag cttacgtttt gcgcttagcg aaagaacatc tgactgaagg
       61 aaaagaatat cgctctgccg gtagaagaaa ctatcttttc agcccagaag cccttaaaaa
      121 actagaaaaa gtcataacca ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa
      181 cgttgaacaa ctagaaaaat tgctagaatc atattccaat gattttatcg aattgaccgg
      241 cggaaaagtc gaagaaacca ccgacctctt tgacctactt ctcacaaata ttctggaatt
      301 aaactctgaa gaacaaaaag agcataaggg aaaactgata gcaccttcct acaggaaggt
      361 ggctaagtcg gctgtgccaa ctggttttct tacaaaacaa ccttatattt tttgatatca
      421 caaaaaaatc cattttggac ctattttcgg tcataatata gtacctactt ttggtcatag
      481 tttcgtccgt agttttggtc atagtgacta agactttcac gcacaaaaaa agagctgaaa
      541 ttaacagctc ttatccttta tttatttttg aatttacact tccaccatga cgattcgatt
      601 ttatatattg cccgataata taagctgaat tatcacaaag aagcggaaac caatcatcaa
      661 atctttctga cattgcatag tcaattaaat ctttgatttc agtgatattc ttagaatcaa
      721 caaacgacat catctctcta atcaactcat atcgttctgc actcgttact gacaaataac
      781 tagcaatttc tgcaccacca tgagcgataa tatcactttt agcatattga aatttttctg
      841 gattatccat atgagcaaaa tatcgcacca taccttttgc attagcacat ttttgcgggt
      901 ttggagaccg aagcttttga gttatctcac gtatttgatc atacgtcttt ttaccgctaa
      961 acattatcaa aacatgccaa tgaggctttt tcaattcacc gtcaggattc acatctttat
     1021 catgaagtgg actctcaacc caaggaacat gtaactcatc taaaaactcc ctccaatttt
     1081 caggagccga ttcaggatag actacaaacg tccaattcct tgtgcgttca tctttagttt
     1141 ttctactcgt catgctataa ttaccttaat tgaacaacag tctaacagca aaagcagtgc
     1201 gccaacacta atgcttttgc tatttttttt gctcattttc ctttccgtta gcattaaccc
     1261 aaattgtaat aagaccagac ttactaagac ccatttgttt cgcaatttta tcaagattat
     1321 ctgccacttc cttagtaagc gttacaacga ctctcttttt ttcattactg atcataattc
     1381 gtatcctcca acgacttttt cttaaaaacc atagtaacaa aaaagtaact aaggtgcaac
     1441 agtttagata ctaattagcg aataataggc cttaaaccct tggcattact gattttgtca
     1501 aaaaaaaacg ctgaaaccct tgggagagta aggctaaaca gctatcgacc aatgaccaat
     1561 gaccaataag gggggtgtcg tagtcccccc ctcctccgcc caattcataa acaccaaaac
     1621 caaaaaacaa acctcagggc tccgccccga accccgacct ccttctaccg aaatatatgc
     1681 aggcgccacg cttactattt cgttcagcta tcggcttttg caatctaaca cgaccgtttc
     1741 gctaccagtc gccttacagg ctccattctc gcttctctta cgtgttttaa cgattgcgcc
     1801 gataactaaa cgaaatagac gctaaatcgc ttaaaacgca aaatagaccc tttttagagc
     1861 attgattttt tctactagtc atttcgataa tataaaattg accacaacac agaaaactta
     1921 tattataagc ttatgtcgta agtccgacgt actcacgtaa tatagacgga cgttaaatgg
     1981 aaggagcata aatgagagtg ataaaatcag aaaaattcac tttggccgaa attatgacga
     2041 ccagcgaact tgcaca
//
