LOCUS       NZ_CP071965             4465 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterococcus faecium strain VB13828 plasmid p7VB13828, complete
            sequence.
ACCESSION   NZ_CP071965
VERSION     NZ_CP071965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18353823
            Assembly: GCF_017603725.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Complete genome of vancomycin resistant E. faecium_VB13828
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: CANU v. v.1.7; UNICYCLER HYBRID v. v.0.4.6
            Assembly Name                     :: VB13828
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017603725.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:36:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,851
            CDSs (with protein)               :: 2,851
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 52 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 38 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071965.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB13828"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p7VB13828"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2020"
                     /collected_by="Christian medical college"
     gene            complement(join(3965..4465,1..39))
                     /locus_tag="J4767_RS15330"
                     /old_locus_tag="J4767_15330"
     CDS             complement(join(3965..4465,1..39))
                     /locus_tag="J4767_RS15330"
                     /old_locus_tag="J4767_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(55..978)
                     /locus_tag="J4767_RS15335"
                     /old_locus_tag="J4767_15335"
     CDS             complement(55..978)
                     /locus_tag="J4767_RS15335"
                     /old_locus_tag="J4767_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            complement(2268..2702)
                     /gene="bacA"
                     /locus_tag="J4767_RS15340"
                     /old_locus_tag="J4767_15340"
     CDS             complement(2268..2702)
                     /gene="bacA"
                     /locus_tag="J4767_RS15340"
                     /old_locus_tag="J4767_15340"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(3107..3280)
                     /locus_tag="J4767_RS15345"
                     /old_locus_tag="J4767_15345"
     CDS             complement(3107..3280)
                     /locus_tag="J4767_RS15345"
                     /old_locus_tag="J4767_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     Typing          complement(56..978)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taattcttcc gcaagttctt tgattgtttt atctgccata aatactcctt aagcttaatc
       61 gaataagttt tgtaaatttc ctagtaaatc ttgtttgagt ttgtttgaaa gttctcgttt
      121 ttctgcctgt ttttcttcag tttgttgttg ttgtttaaaa tcagcttcat gagtgcctaa
      181 gggaagattt agtattcgat ctttagcgcg ccatttttct tctcgtgtta attctccatt
      241 gtgatcaata ttaaataatt ttatacgtaa atcttcttgt ttccctttgg aaaaatcgtc
      301 agcatggtta atttcagctt tccaagtaaa acgataaccg ataacaggtt tacctcgacc
      361 tttaccatat tttttacgaa tagttaatcc tttaaataag ggagtgagtt cttctttaat
      421 tggccttaag ataaatttgt caatatttga cggactattc caataacttt taggaatatc
      481 tagtaaatca aaaaattttt ctttggaaaa ttcagtccag ccttgagttc gatattgttt
      541 taataatcga aacatagttt ttgaatatgt gctatccaaa ttagtaaatt gctctagatt
      601 aaatcttgtc cagttcttta attgattaaa aagtccttta aattctggat taactgaaat
      661 tataacaagt tgtttatctc gtataatgct atattttgtg aaagcaacaa atttagttaa
      721 agtatttcca tcatcattcc aagcattaat acttaataat ttgtcatacg tgctactaag
      781 atcttttata aattgttccc cacgttctgt ataaccgctt agttttttca aatcgtgaaa
      841 tgaaaaaata atctcatcta cacctttttc acgtacacgt gaagcaattg aaaaaaataa
      901 gttcatttca cgactattaa attttctcag aggaatatga tttaatctat tttcatattt
      961 tacaatttca tttgccataa gcttctccta cttttataaa gtatgaaata aaagtaacat
     1021 actttataaa aatactcaat aatgtcggaa aacctacttg aaaagcgtag aaaacctact
     1081 tgaaaagcgt agaaaaccta cttgaaaagc gtagaaaacc tacttgaaaa aactctgaat
     1141 tgcttgggag agtaaggaca cagccgacat aaaagagata taaaaagaga tataaaaaga
     1201 gatataaaaa gaggggaggc taattttccc gcctaaaaaa tcactccttt tttctctaaa
     1261 caggttcgcg aatttatcaa aactgccaat tccaaacctt gctccctacg gttcgctaaa
     1321 aaggtgtgat cgctctgctc tcacattata ctgcgcagtc gcctccggct cctttgctaa
     1381 aagccgttca taaggaaccc tttgagaagc tctgagcgtt cgtttaagac attttaagac
     1441 agtcctagta taaatccctt atcttgttct taaaacgcct tacaggcggt cagggcaagc
     1501 cctaacaacc ctaaaattgt ctaaatgccc tctaaaattc gatttaagca ctcttaaacc
     1561 tattcttata tatttatata cttaggcgtt tcaaacgtct taaaacgcaa attagagctt
     1621 gataaagata tttgctcgtg tgcctagact tttaaaaaag tcggcacacg ccatgacaaa
     1681 gctgaaataa actgcttcag ctttgtcatg gcggattcgc tcacttgaaa aagtgtttat
     1741 ttcgctcgat tattagcata tattttttag cttttttgct agccaaattt tgcattaatt
     1801 tgctcataag tcccaccctt tcagtacgtc ctacggacgt atcgttcgta atgtgaaagt
     1861 acctttaaag gtactttcta caaaactcac taaaagggtt tggcaagctc tatatttgct
     1921 agccaaatac ctccgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc
     1981 ttgaatcccc ctcaaaaccc acgctccaca atacaaaaag tggctagaaa atgctagcca
     2041 ctttttgtaa aatttagtat gatttatttt tagctattta gagtttttat ttccgtaaat
     2101 acttctaaaa attgctaaga taaatgctat aggaattaga taaagaatac ttttttgtaa
     2161 gcttatattg aaaattagcc taaatatgaa gaacatagtg attaaaacta ttattgaaat
     2221 aattacagct aaaatctttt tattcatgag atagtttcac tttctgatta ttttattaca
     2281 taacgagcgt aatcattgta ttgatatgat gttgtattgc taaagtgagc attatatgtt
     2341 aattgtacac cttttccttg atttgcagct cttacaaaga cattcattgc atcaacagca
     2401 ctactaaaag catttgtaat aacccatgtt ccaactccac caataggaat aaaagatgca
     2461 atttgagcag gagtctttag cttattataa ttttttttca aatctctaca cattttatta
     2521 gtaacataat agttcatttt atagcaagta aatccacgat gattatgatt atatcgtgaa
     2581 cttgcagctt ccacttttat acctctagca ctaaaaggaa acagatttaa cggagtactt
     2641 gctccaacaa tcataccgaa caaaaataga caagtaataa ttttaataat tttatttttc
     2701 attgttactt cctccttaga tataaaatcc taggaaagcg ctttctgtat atatttagaa
     2761 taatcagaaa attaaatatt gtcaagaggg tttattttat aaaacgtgtt attttaaagg
     2821 ttagtagaca taatgtgtac tatgccaagt agcctctcta ccaagggata caggggctca
     2881 ctttgtgatt ttcgattggt ctaagtcaaa agctcttttt aaggcttgat tttctgcagt
     2941 tgcttctttc cgtttctttg tgaattttta ccagtcattt tttactgggg tgttattttt
     3001 cataaaatat gataaatttt tggtagcgct tcataagcgc gttgcttttt caagaataaa
     3061 cactcattat tttttaactg gctgctcgaa agagcagtct tttttattat ttatccgtct
     3121 cttttacaaa caattcttcg ttaggactgt tgtaataatt tgaatttaat ttaagttctc
     3181 cgttggaatt gttttttcca attacggata atagcatggg gctaaaacct aatattccta
     3241 gagaaataga acccagtacc aacacagttt tttttaacat aggttatacc tccacttttt
     3301 ttaacgctac cacttgtata cacattttaa atactagcct tcagtttatc aaatgtttaa
     3361 attgaacgga tataaatcaa aattaaattt tcaaaagttg ttctaaaaaa tttttctttt
     3421 ttggaagtta ctctaacttt ttttgcttgt ggtgcttggt atgttttctt cgccgaccgt
     3481 ttaggttctt gaccattttc tcttataagt gggattttta ccacgttgag agaaaatggt
     3541 caagaacctg ggaggcggag aatgccctta ataccaagca ccgcaagcct ttcccatact
     3601 ttattcgatg atcccacgat gatctcataa tgattctaca atgaccctac gatgatcaaa
     3661 attctcaaaa aaaataaacg aaatttcaaa cgattttttt gaaattttgt taaaatcaca
     3721 tttgtttttt tatgactttt tcaacaaaat cgattagaat ttattttgtt tttccaccaa
     3781 aaattgagtg agatttcaac ctattttaag caataagtca ccatatagtt tgatagttta
     3841 caactataaa cattgattgt ttacagttgt aaactatttt ttctttctca taaaaatttg
     3901 agtgatctcc ccatcaggta gtacaacttt ttcctctact atccgaacta acttctagaa
     3961 aattttagag attggttcta ttagatttcc aaatttgcca ccactttttt tcaataagtg
     4021 gttgtgtatt gtctgctttt ttttcagaaa aggcagttga ttcggttgtt ggagtttctt
     4081 ctgtataagt taaatggagc tgttcttgta ggttctcaat ttgttggttc gcttgtagag
     4141 tgagttgttg ttgctggtcg agtaattttt gcatttgttt tatttgatta tctttttctt
     4201 gtagctgtat atcttttcga tttaactgtt tttctaggaa agaaaggagt tttaattcgg
     4261 aaacttggtt ggtgttttta tggtttttgg tttttgattt ttcgccaact agttttcgat
     4321 ttaagtttgc gttatcgact aaaaacattg attttatagc tttttgaccg gtttcgttaa
     4381 tcacaaactt gtttccgttt tttgcaaacc aaagttttcg atttttgtca gaaacttttt
     4441 tgttaattgc tgttttacta actcc
//
