LOCUS       NZ_CP071962             9934 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterococcus faecium strain VB13828 plasmid p4VB13828, complete
            sequence.
ACCESSION   NZ_CP071962
VERSION     NZ_CP071962.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18353823
            Assembly: GCF_017603725.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Complete genome of vancomycin resistant E. faecium_VB13828
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: CANU v. v.1.7; UNICYCLER HYBRID v. v.0.4.6
            Assembly Name                     :: VB13828
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017603725.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:36:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,851
            CDSs (with protein)               :: 2,851
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 52 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 38 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071962.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB13828"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4VB13828"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2020"
                     /collected_by="Christian medical college"
     gene            1..933
                     /locus_tag="J4767_RS15210"
                     /old_locus_tag="J4767_15210"
     CDS             1..933
                     /locus_tag="J4767_RS15210"
                     /old_locus_tag="J4767_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="J4767_RS15215"
                     /old_locus_tag="J4767_15215"
     CDS             950..1420
                     /locus_tag="J4767_RS15215"
                     /old_locus_tag="J4767_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="J4767_RS15220"
                     /old_locus_tag="J4767_15220"
     CDS             1504..1956
                     /locus_tag="J4767_RS15220"
                     /old_locus_tag="J4767_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_060806732.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="J4767_RS15225"
                     /old_locus_tag="J4767_15225"
     CDS             2083..3264
                     /locus_tag="J4767_RS15225"
                     /old_locus_tag="J4767_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3354..3623
                     /locus_tag="J4767_RS15230"
                     /old_locus_tag="J4767_15230"
     CDS             3354..3623
                     /locus_tag="J4767_RS15230"
                     /old_locus_tag="J4767_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="WP_074398906.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
                     /name="bacteriocin 32"
     gene            4429..4602
                     /locus_tag="J4767_RS15235"
                     /old_locus_tag="J4767_15235"
     CDS             4429..4602
                     /locus_tag="J4767_RS15235"
                     /old_locus_tag="J4767_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162897362.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            5060..6160
                     /locus_tag="J4767_RS15240"
                     /old_locus_tag="J4767_15240"
     CDS             5060..6160
                     /locus_tag="J4767_RS15240"
                     /old_locus_tag="J4767_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_211182505.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            6133..6576
                     /locus_tag="J4767_RS15245"
                     /old_locus_tag="J4767_15245"
     CDS             6133..6576
                     /locus_tag="J4767_RS15245"
                     /old_locus_tag="J4767_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            6878..7258
                     /locus_tag="J4767_RS15250"
                     /old_locus_tag="J4767_15250"
     CDS             6878..7258
                     /locus_tag="J4767_RS15250"
                     /old_locus_tag="J4767_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7240..8154
                     /locus_tag="J4767_RS15255"
                     /old_locus_tag="J4767_15255"
     CDS             7240..8154
                     /locus_tag="J4767_RS15255"
                     /old_locus_tag="J4767_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8114..8392
                     /locus_tag="J4767_RS15850"
     CDS             8114..8392
                     /locus_tag="J4767_RS15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            8394..9083
                     /locus_tag="J4767_RS15260"
                     /old_locus_tag="J4767_15260"
     CDS             8394..9083
                     /locus_tag="J4767_RS15260"
                     /old_locus_tag="J4767_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7241..8151
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatacct taaagttaat cattaaaaaa ggagtgtcat aatttgaaaa
     3361 aaacaaaatt attggtagct agtttgtgtt tgtttagcag tttattagct ttcacccctt
     3421 ctgtttcatt ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct
     3481 acaaaaaata tccaaaaggt gctaaagtgc caaataaagt taaaatgtta gtaaatatta
     3541 gaggtaaaca aactatgcgt acttgttacc ttatgagttg gaatgctagt agtagaacag
     3601 ccaaatatta ttactacatt tagtaaaaac aaaacattct ttctatacaa taaaatagcg
     3661 tatagagagg atgtttttaa tttgaaaagg agtggttatc tttatgaaaa atagatcatt
     3721 tgctatatta gtattatccg taattttatt agatatattg ttaataacat tagctttaaa
     3781 aactttaaat ttagtaacaa gttttgtcat tataccagta attataggaa tgtctattta
     3841 tttcatcaaa aaaaataaac aatttttttg aatgaaatta cgggcgaaaa taatcactca
     3901 aaattattaa taaaaacgaa tcaactactt tttctgaaga aacatacaac cgtaaacaac
     3961 aattgttgtt tacggttgta aattatcaaa ctatatggtg acttgttgct taaaataggt
     4021 tgaaatttcg tttatttttt ttgagaaatt ttacccacgt actacccatg tacctatcca
     4081 cgtattaccc atgtaccccc ccatgacaaa tgggcggagg ggcttgcggc gcttggtatt
     4141 aaaggcatta tccgcctccc aggtttctga ccattttctc tcataagtgg ggtttttacc
     4201 gcgttgagag aaaatggtca gaaacctaaa cgatcggcaa agaaaacata ccaagcgcca
     4261 caagcaaaaa aagttagagt aacttccaga aaagaaaaat ttttagaaca acttttgaaa
     4321 atttgatttt gatttatatc cgttcaattt aaacatttga taaactgaag gctagtattt
     4381 aaaatgtgta tacaagtggt agcgttaaaa aaagtggagg tataacctat gttaaaaaaa
     4441 actgtgttgg tactgggttc tatttctcta ggaatattag gttttagccc catgctatta
     4501 tccgtaattg gaaaaaacaa ttccaacaga gaacttaaat taaattcaaa ttattacaac
     4561 agtcctaacg aagaattgtt tgtaaaacaa acggataaat aataaaaaag actgctcttt
     4621 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
     4681 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
     4741 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagctttaga aagttgcttt
     4801 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     4861 tggcatactc gacattatgt taactaacgc tataaataac gattatgttc ccaaaacaat
     4921 attttatgaa ctataacaaa aatttgttat cgcatattgt atattttaat tataggtgta
     4981 taccacttaa atttaacaca aaggcgtgat tattttgaaa cccaaaaaga aaatttatct
     5041 aggtctcctc ttttccttga tgattggaat ttttagtata ggaattacaa atacctatgc
     5101 ttcagaaaat tctaatttca atatcaactc ccaaccaatt gaagaacaag caattaattt
     5161 tgcgaaaact gattttgaac actccataac ttcaattctt ttaatggaaa ataacaccga
     5221 aactgatatt aactatactc ttggttcgcc atttaaaatg ctcgatggag aaaatacggc
     5281 aaatagttct attttctttc cagtattttc agacgctgat aatacgatta aatatattta
     5341 taccgtcaaa aaaaatacaa atggagaata ttcaggaagt atttcacaat ttttagcaaa
     5401 agaacttcaa aatctagtag atagtaatat aacagataca accttatttt ttcaagataa
     5461 tagcatttat tattccgata atggacaaat taaactactt tggaaatcac ctttaagtac
     5521 agaaaaaaat ggtgtttctt cctttacaga aaaaaatgat cttatatccg taaatataaa
     5581 aaataaaatt aaaaatttct ctaaaaaaca aaattttcct gcgattaaac gtgtaaaaag
     5641 tgtctcctcc ggagataact ttattatgat taattggcgt atagcagaaa cccaaacaag
     5701 ttttccgtgg tgtgctgctt acgtagaagc tgctatctta tgtaacaaaa cagatgtaca
     5761 tgctacttca gctagagcca ttatgcatta tacctatcct aatttaaaca acagtcaaat
     5821 tgaatcaaaa agtcttaatc aagatcaaat aataaaatat gcaaattact gtggtgttta
     5881 tcctaaaaaa gtagcacgaa tactaaacct ttcagaggtc caaacacaac taagaaaagg
     5941 aaatgccatt tatatgggaa ctgctggtta tggaaattat agtaacagcc gtcatgcact
     6001 tgcattattt gggtgggtca atcaaggtgg ttatcaaact tactatgtgt ggaatccttg
     6061 gtataactac ccaactgtaa taagtgcaaa tgcaggacca gttacaattc ctgtacctgg
     6121 aggtggtttt acttgggaca ataccattac aaattggtaa ataaacccat tgctttagtt
     6181 atgggtctag tgttagttct tttcttaact agttgttcaa aaaaacaaga aatacaaaag
     6241 actgatctga ttccttttct aggaacttgg caaactaaag atcattcaga acgtctagta
     6301 atcactaatt taaatacttc aaaaaatata gaacaatata ccatcttagt aaaagagaac
     6361 aatcataaaa ttcaaatcat tgataaaaat ttaaatataa atgaacaagt tttcatttac
     6421 aaagcaacaa ataatttact aacactgcgc tctaataaaa aacaattaga ttacactttt
     6481 agtttggatt ctccaaaaaa gctcgtattt attttgacaa cgaatgatgg gacagaaggg
     6541 acagctaaac caattgaatt ttttaaagct gattgatgaa tagttgtttg atattacaaa
     6601 acaataattt caccgaaggt gaaacaagcg aaactttaca aaaagtggct agcatttgct
     6661 agcctttttt gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc
     6721 ctttgaaaag cggttatttg caccaaatac gtaggtattt ggctagcaaa tatagagctt
     6781 gccaaaccct tttagtgagc tttactcatt gtaccttttt aggtacaatg gtattgcgaa
     6841 cgatacgtcc gtaggacgta ctgaaagggt gggacttatg agcgaacaaa atcaaaattt
     6901 ggctagcgat cattctaaaa aatataccta tcgatccgag ccaaaacaaa tcagttttcg
     6961 agtgagcgaa tctgaatttg caaagctgaa gcagtcagct gaagctttgc aaatgagtgt
     7021 gccggctttc gtaaaagcca aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc
     7081 agacattgcc caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat
     7141 tgctaagtgg tgtaatacac accaacacga cgttgctcct ggagacgccc aacgcttatc
     7201 cgaaaaccta aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagc
     7261 cgaaccactt cttgtagtcg tgcaattaat tacgctgaac cacgagcaac cattaaaaca
     7321 ggaataaact gtgacgtcga ttatgccaaa agtgaaatga aacaaattcg catgctgtat
     7381 ggaaaagacg accgtgtaca ggcacactta ttgattcaat cttttcgacc aggagaaatt
     7441 accgctgaaa aagctaatca actaggggca gagtatgcaa aaaaaatagc ccccgatcac
     7501 caaatagcta tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac
     7561 tccgttaatc tggagactgg aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa
     7621 tgttatgacc taaatgacga gatttgtctt gcacataact taccaatcac tgagcgagga
     7681 aagaaaccag aaaaacgaac catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt
     7741 tggaaagacg aaattcggat tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc
     7801 tatgaccaat tttgtaattc cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa
     7861 aatttcactt acgaattgat ccacaaaaag aaaaaagtac gatccaacag actaggaaag
     7921 gactacgaaa aggagacgat tttacatgag ctggacagac gagaacgaac ctataaccaa
     7981 cagcgaacca acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta
     8041 cgaagagaac aacgaactct tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac
     8101 catgaacaac acgttgaaag aggaacagac caccaacaag aattatcgct ctaaaacgtt
     8161 aaaagacata gaaaacgaaa taaagaaaca acgtgctttt ctcgttcctt atacgcaaga
     8221 aaggatacaa actacgattg atttacaagt taaaaaactc catcaagagc tattagacca
     8281 gcgaaataag gaacagaaaa aggtgcttat tcaacgatca ttggattttc ttgccccctt
     8341 atgttgctta gtcctgacac tgttcgcatt ttggttagta tggggaggct aaaatggcat
     8401 tttatttacc ctatctactc atttttgtta gtatttcagg aagtatttgg ctcatctata
     8461 aaatttttca aacaagatat tcattaaaag gctctaaaat tcgatttaag cgatttttct
     8521 ttcttggctg tatattttcc ttgataatcg tttcaagcgg cttattcggc gttttagagg
     8581 gaaataagcg cgtttctaga agtattttat taggaaatgt cactcaaaag tatgaatcag
     8641 cgcgtgataa aaagaaaaaa gagcaagtat tagcacaaaa aatagaaaaa tttaccgctt
     8701 gttatgagga catgaatgat atttttgtga agcaagaaaa gcgtttgacg gataaaaata
     8761 tggaaacact cactcgactt tatcggaatt tgccagaaga acagcaaaaa gagtaccaag
     8821 aaaaatatga acaagtaaaa aaagacgtgc aatatgtgaa agatacgaaa gtggaagaag
     8881 cttgtagtga tttatttgga gatacaattc ctttttcaac tagcgaacaa gaacgaaaag
     8941 aacgacaaca atcagtcaca tatgaacgct atgaaatctt gcttcaacaa gcaattaaca
     9001 tacaaaatct gactaaaaaa gaaacagctt taaattattt aaaaagcgtc aaggaatggt
     9061 tagatcagca acagcaaaat tgaatttgat tgaaattcat gttaaactaa acatgcaaat
     9121 gttcaaacat tagatacacg aaagccccag agaggtcgga ttctctgggg ctttttttag
     9181 gttgatgtcg cctttactga ttttacttct tgtttcggtt attcaaccaa taactgaata
     9241 cagagataca aactcctaca agaagggggc aaataaacag ttcaaacatc agatacacct
     9301 ccttccagtc gcaagactgt cggataggcg acaagttatt ataccctaca aaattaaatc
     9361 tgtgtggcaa ttttaagtat ctcttatttc cgggttgtta gggcttgccc tgaccgcctg
     9421 tagggcgctt taaatgccag ataaaggttt ttatactaag actgtcttaa aacggcttag
     9481 acaagctctt agagcctcaa ccctcctttt tgaacaatgg ttttagcaaa ggagctggag
     9541 gcgactgcgc aggtataatg tgagagcgta gcgatcacac ctttttggcg accgtaggga
     9601 gcgaggtttt gaattagcag tttagaaaag ttcgcgaacc tgtttaaaga aaaaataatt
     9661 gtttttttag gtgggaaatt ttgtctccct cttttataat ctttttttaa acctcttttt
     9721 aaacctcttt accgtcggct gtgtccttac tcccccaagg gatacaaaat tttataggca
     9781 tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat tatctgttta
     9841 taggcatgaa attatctgtt ttaggtattg tttttagaca taatatgtcc taaaatgatg
     9901 tcaataaaat catttagact ggagtaacaa ctaa
//
