LOCUS       NZ_CP071961            10238 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterococcus faecium strain VB13828 plasmid p3VB13828, complete
            sequence.
ACCESSION   NZ_CP071961
VERSION     NZ_CP071961.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18353823
            Assembly: GCF_017603725.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Complete genome of vancomycin resistant E. faecium_VB13828
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   Balaji,V., Yamuna,B.D. and Anushree,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: CANU v. v.1.7; UNICYCLER HYBRID v. v.0.4.6
            Assembly Name                     :: VB13828
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017603725.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:36:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,851
            CDSs (with protein)               :: 2,851
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 52 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 38 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071961.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB13828"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p3VB13828"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2020"
                     /collected_by="Christian medical college"
     gene            join(9483..10238,1..441)
                     /locus_tag="J4767_RS15170"
                     /old_locus_tag="J4767_15170"
     CDS             join(9483..10238,1..441)
                     /locus_tag="J4767_RS15170"
                     /old_locus_tag="J4767_15170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_013330756.1"
                     /translation="MSIISENQKQVYTLNEIEKRKVVEHNSLITSIAKMQKTALKMFEL
                     AVSCIDTENPPKDNIIYLSKKELFSFFDVSSASKHTRFKEAIEYMQKQAFFQIKEEKNK
                     GYEMTSIVPIPTVKWNSYNDDVMIQFNQFIMPYLIDLKAEFTQYKISELKELNSKYSLI
                     LYRWLAMNYSQYEHYSVKGGRRAEQVEAYRNPSITVKELREITDTINEYTRFQSFENRV
                     LKNAIEEINAHTSFNVSYEKVKKGRSIDSIVFHIEKKRRADDNSYKLEDTAFQVDKKQK
                     EQSEAVLVKQAMESKYTRLLLDNMLLSPYEMTDTSLMAGLQAHVYPLYDELKALRGLNG
                     VKDHLSYVRAKQEAYSKRNIAKYLKKAIEQYLPTVKLQDLEQPERASVRGRGKGEIHE"
                     /name="RepB family plasmid replication initiator protein"
     gene            434..1006
                     /locus_tag="J4767_RS15175"
                     /old_locus_tag="J4767_15175"
     CDS             434..1006
                     /locus_tag="J4767_RS15175"
                     /old_locus_tag="J4767_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_089202027.1"
                     /translation="MSENLKTIKELADELSVTKQNIQYHYQRLPKELQLKSSNGSNLIN
                     SKAEKIILGKVESGSKSNTKDQQISSKENFEDEKLDNPFINRLIKEVEYLRIDKDKIIS
                     TKDQQISSKDQQIEKLTNLLDQQQRLALQDKKLLEEYKEEIKELKSLMLPVREDEKDPE
                     AHPSKDINEIKEEPEKKNKKWWHFGRM"
                     /name="DNA-binding protein"
     gene            1055..1747
                     /locus_tag="J4767_RS15180"
                     /old_locus_tag="J4767_15180"
     CDS             1055..1747
                     /locus_tag="J4767_RS15180"
                     /old_locus_tag="J4767_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570254.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_081117314.1"
                     /translation="MDDEKLIKSKERVQQHGEVFTPQWMVKKMLDVEGIKQACENIDAT
                     FLEPAAGDGNFLVAILERKLKVVTEQFSEGHWGTKSLFALSSIYGIEFLADNLEIARSR
                     MLIHYLNWYEEIFQEKLHSTTDLYKSAMYIIRRNIVRGNTLTKKHPDYDIPIMFNEWKK
                     VKGSSSKVEKIEFTFSSLFENEEVLEKGISEGQLSLFDFEMFDEENKEKKDDSVIIDIK
                     KIYKLGEG"
                     /name="N-6 DNA methylase"
     gene            1749..6137
                     /locus_tag="J4767_RS15185"
                     /old_locus_tag="J4767_15185"
     CDS             1749..6137
                     /locus_tag="J4767_RS15185"
                     /old_locus_tag="J4767_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570255.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]; GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="WP_169043412.1"
                     /translation="MSNFEFLTLELETAELFQTANFAEKNYTEKDYEGTLTKVRKLAEN
                     TANEIAIKEHIVLSERSSFNDVLRELKNDSIDQFIIESFYEIKRLGNDSAHNLNSRSAT
                     QENAKQALHKIFIILVWYMNHYFEIDIKTAYLDFLEPQAEKLYQTAERKLIYIQTADNT
                     SGMFPAYKDAQKIGEATAPDDDFEVDWSPNSEFLRTVGAKRINQYMKTSGIKFVLEWVE
                     LAWKKSTKTWFHDHDVHEVLKRSGIKRPEFLDGSEWFETDVETAKSAIKAVKEGQSALD
                     SVGSTNADDHITLRPEQSLAVEKTRKNFKKNKKMLWNAKMRFGKTLTSLELIKEQKYTK
                     VLIMTHRPVVSDSWFEDFGKMKMAEEGYRYGSEDTQFKLKDLKSGNTPFIYFVSIQKLR
                     YGGGKVNLREFTDVDWDLIIIDEAHEGTQTELADNVLKSLEKDNTKILSLSGTPFNIQD
                     QYSDESVYTWDYTMEQSAKLRYSLEHPTEKNPYESLPKVNMFTFEMKNKERFSDNSKSF
                     NFREFFRVNENNEFVHKHDINAFLDNITNQDSSTNYPFSTKQYRNELRHTLWLLPGVKE
                     ANAFEKLLNEHQIFGKEYKIVNVVKDDKSDSNEVATEGDLDKVRQAIGDPSQSKTITLT
                     VRKLTTGVNIPEWTAVLFLSNTNSAMNYLQAAFRAQTPFSHEKLGMKKNCYIFDFAPDR
                     ALTVMAESAQINSGVGKKNTLQQKEAMTQLLNFMPILGQTEHGMKVFNVDRMLTQLKKV
                     YAEKAVRSGFEDDSLYNDELLTLDEADLNDFNNLKEIVGKTNLSGLPKKVEINVNGLTD
                     EEYEKGEKAQKKKPRERTTEEKEIIEKVKQAKKQRKTMISILRGISIRIPMMIYGMPIE
                     VDKEMGIDEFVNHVDSISWEEFMPKGIKKSDFKRFAKYYDPEVFVEAGRIIRQRAQSYD
                     GLEYTERAEKIAELFGTFKNPDKETVLTPWRVVNLQLSKTIGGLCYFDENFENTTLNGQ
                     DSITWVDTDITKEVFKSDTKILEINSKTGLYPLYVASSLFYQNRNKLNDDRAGRFSKID
                     EDEIIQEVLKENIYVIAKTPMAKTITQRTLAGYNNWATNILYVEDINQKLKTNMDKTIE
                     EIQKGLNVMKFDVVIGNPPYQEKNIGNSTQAPPIYNKFMDLSYSIADKAVLITPARFLF
                     NAGATSKAWNEKMLNDKHLKVLFYERDAAKVFSNTTITGGVAITYRDSKKDFGAIKIFT
                     NYEDLNLIVKKVENKNPVSFSTIFYGQNAYQFLSVFHEENPQAIQKMSKGHEKDIITNV
                     FDTLNFAFYNEIPDDDKYIKIYGRQNNKRVSKYIKAKYIKNHESIDKWKVFIPKANGAS
                     GMITDSGLSRIISTPFIGEPFSGHTQTYISVGKFDSEEEAENCLKYIKTKFARLLLGVL
                     KVTQDNPGPKWKYVPLQDFTNHSDIDWTKSISDIDNQLYKKYDLDLQEIKFIEDKIENM
                     D"
                     /name="Eco57I restriction-modification methylase
                     domain-containing protein"
     gene            6402..6983
                     /locus_tag="J4767_RS15190"
                     /old_locus_tag="J4767_15190"
     CDS             6402..6983
                     /locus_tag="J4767_RS15190"
                     /old_locus_tag="J4767_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="WP_089202025.1"
                     /translation="MNRFFKPIKYLGILTVVIVISAVITYIIDPNLSDTLNSVSSNVPK
                     SVSQKSGLQLVVAYIFNNGFKVPIGMLILSIIPIRFLYWIQPLFTVILPGILFGIVFRY
                     SLAKGFVILISSLPHMLFEVFAFCVWMVALDQFNKWIRYKISRKKQTNTKLFYEFKLIL
                     ISYVKYVIPLIVIAAFTETYVADWISHILS"
                     /name="stage II sporulation protein M"
     gene            6999..7589
                     /locus_tag="J4767_RS15195"
                     /old_locus_tag="J4767_15195"
     CDS             6999..7589
                     /locus_tag="J4767_RS15195"
                     /old_locus_tag="J4767_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_169043413.1"
                     /translation="MVQQIVLPIKDSNILKMVQDTLLDSFRAGRRNYTIFQVGKATLLR
                     VSDVMKLKKTDVFNPDGTVKQTAFIHDQKTGKANTLYLKPVQQDLLIYHDWLVQQNLNS
                     EWLFPSTSRSDRHITEKQFYKIMTRVGELLGINYLGTHTMRKTGAYRVYTQSNYNIGLV
                     MHLLNHSSEAMTLTYLGLDQASRETMLDQIDFG"
                     /name="site-specific integrase"
     gene            7762..8004
                     /locus_tag="J4767_RS15200"
                     /old_locus_tag="J4767_15200"
     CDS             7762..8004
                     /locus_tag="J4767_RS15200"
                     /old_locus_tag="J4767_15200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="WP_208572923.1"
                     /translation="MILEKRGTISEEENRLFFFSSFCMTKRKIFCPFYFYKMGRWRLRK
                     ANRHNPKGIKGESETSTFSSHIVIQERKVLLLMIG"
                     /name="cytochrome B"
     gene            complement(8327..8590)
                     /locus_tag="J4767_RS15205"
                     /old_locus_tag="J4767_15205"
     CDS             complement(8327..8590)
                     /locus_tag="J4767_RS15205"
                     /old_locus_tag="J4767_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin epsilon"
                     /protein_id="WP_169043411.1"
                     /translation="MTLDYRKTFEIEIINEFQSAIHSKMLNYVLNNELDKSDSTNLQTN
                     LLNQLSSMNQINLFKLSLEELEAYHDYLRAIKKYADSITRTT"
                     /name="antitoxin epsilon"
     Typing          2..351
                     /name="rep_cluster_570"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.43"
                     /coverage="100.0"
                     /evalue="5.77e-180"
ORIGIN
        1 gtttttcata ttgagaagaa acgccgagcg gacgataaca gctacaagtt ggaagatacg
       61 gcttttcaag tagataaaaa gcaaaaagaa caaagcgaag cggttctagt taagcaggca
      121 atggaaagca agtacacacg gttattgttg gataatatgt tattaagtcc ttatgaaatg
      181 accgatacgt cacttatggc aggattgcag gcgcacgtgt accctctcta tgacgagctg
      241 aaagccttac gagggttgaa tggggtcaaa gaccacctct cttatgtgag agctaaacaa
      301 gaagcctatt caaaacgtaa tatagctaag tatctaaaaa aagcaattga acaatacctg
      361 ccaactgtaa aattacaaga ccttgaacaa ccagaacgtg caagtgttcg aggtagaggt
      421 aaaggagaaa tccatgagtg aaaatttaaa aacgatcaaa gagctggcgg atgagttgag
      481 tgttacaaaa caaaatattc aatatcacta ccaaaggtta ccaaaagaat tacagcttaa
      541 aagttccaat gggtctaatc taataaattc taaagcagaa aaaataattt taggtaaagt
      601 agaaagtggt agcaaatcaa atacaaaaga ccaacaaata agtagcaaag aaaattttga
      661 agatgaaaaa ttagataatc cctttatcaa tagattaatc aaagaagttg agtatttgag
      721 aatagacaag gataaaatca tatctaccaa agaccaacaa ataagtagca aagaccaaca
      781 aatagaaaaa ctaactaatt tgttagacca gcaacaacgt ttagccttac aagataaaaa
      841 attacttgaa gaatataaag aagaaattaa ggaattaaaa tctttgatgt taccagtacg
      901 agaagatgaa aaagatccgg aggcacaccc atctaaggac ataaatgaaa taaaagaaga
      961 acccgagaaa aaaaataaaa aatggtggca ttttggaaga atgtgaaagt atataaggat
     1021 aactaaattt ttttagtaag actggaggaa tattgtggac gacgaaaaat taattaaatc
     1081 aaaagaacgt gtccaacaac atggagaagt gtttacccct caatggatgg taaaaaaaat
     1141 gttagatgtt gaggggatta agcaagcttg cgaaaatata gatgctacct ttttagaacc
     1201 tgctgctgga gatggaaatt tcttagtggc tattttagaa cgtaaattga aagttgtcac
     1261 tgaacaattc agtgaggggc attgggggac aaaatcattg tttgcgttga gttctattta
     1321 tggaattgag tttttagcag ataacttaga aatagctcgc tctcgtatgt taatccatta
     1381 tctaaattgg tatgaagaaa tttttcaaga aaaacttcac agtacaacag atctatataa
     1441 gtctgctatg tatatcatac ggagaaatat cgtccgagga aatacgttaa ctaagaagca
     1501 ccctgattat gatatcccga ttatgtttaa tgaatggaaa aaagttaaag gttctagtag
     1561 taaagtggaa aaaattgagt tcacattttc aagtttattt gaaaatgaag aagtgttaga
     1621 aaaaggaatc tcagaaggcc aacttagttt atttgatttt gaaatgtttg atgaagaaaa
     1681 caaagagaaa aaggatgatt cagtcataat agatattaag aaaatatata agttgggaga
     1741 gggatgatat gtcaaatttt gaatttttaa ctttagaact agaaacagct gaattatttc
     1801 aaacggctaa ctttgctgaa aaaaattata cggaaaaaga ttatgaaggt acgcttacaa
     1861 aagtcaggaa attagctgag aatactgcca atgaaattgc aataaaagaa catattgtat
     1921 tatcagaacg ctctagtttt aatgatgttt tacgtgaact taaaaatgat tcaattgatc
     1981 aatttataat tgaatcattt tatgaaatta aacgtctagg aaatgattca gcgcacaatc
     2041 taaatagccg tagtgctaca caggaaaacg ctaaacaagc actccataaa atttttataa
     2101 ttttagtttg gtatatgaat cattattttg aaattgatat taaaacagca tatttagatt
     2161 tcttagagcc acaagctgag aaactctatc aaacagcaga gcgtaaatta atttatattc
     2221 aaacagccga taacacctct ggaatgtttc cagcttataa ggatgcacaa aaaataggag
     2281 aagctacagc tcctgatgat gattttgaag ttgattggtc tccaaacagc gaatttttaa
     2341 gaacagtagg cgcaaaaaga atcaatcaat atatgaaaac ttcagggatt aaatttgtcc
     2401 ttgaatgggt cgaattagca tggaaaaaat caaccaaaac gtggtttcat gatcatgatg
     2461 ttcatgaagt actgaaacgt tcaggaataa agcgtcccga attcctagat ggatccgaat
     2521 ggtttgaaac agatgtagaa acagcaaagt cagcaattaa agcagtaaaa gaagggcaat
     2581 ctgcacttga tagtgttgga agtacaaatg ctgacgatca tattacactt cgcccagaac
     2641 aatcattagc ggtagaaaaa actcgaaaaa atttcaaaaa aaataaaaaa atgctatgga
     2701 atgccaaaat gcgatttgga aaaactttga cttccttaga acttataaaa gaacaaaaat
     2761 ataccaaagt tttgattatg acacatcgac ctgtagtttc tgattcatgg tttgaggatt
     2821 ttggaaagat gaagatggca gaggaaggct atcgctatgg atcagaagat acgcagttta
     2881 agttaaaaga tttgaaatca ggaaacactc catttattta ttttgtgtct attcaaaagc
     2941 tccgatatgg cggaggaaaa gtaaatttaa gagaatttac tgatgttgat tgggatttaa
     3001 ttatcattga cgaagcacat gaagggacac agactgagct tgcagataat gtattgaaat
     3061 cacttgaaaa agacaacaca aaaattttgt cattgagcgg aactccattc aatattcaag
     3121 atcagtatag tgatgaatct gtttatacat gggattacac catggaacaa tctgcaaaac
     3181 tccgttattc attagaacat ccgacagaga aaaatccata tgaatcatta ccaaaagtta
     3241 atatgtttac gtttgagatg aaaaataaag aacgcttttc ggataattca aaatcgttta
     3301 atttccgtga gtttttccga gtgaatgaaa ataatgagtt tgttcataaa catgatatta
     3361 atgcattctt agataatatt actaatcaag atagtagcac taattatcca ttttcaacta
     3421 aacaataccg taatgaatta cgccatacat tatggttatt accgggagtt aaagaagcga
     3481 acgcttttga aaaattactc aatgaacatc agatttttgg aaaggaatac aaaattgtta
     3541 atgttgtgaa agatgacaaa tcagatagca acgaagtcgc aacagaaggc gatttggata
     3601 aagttcggca agcaatagga gatcctagtc agagtaaaac tattacactt actgttcgta
     3661 aattaacaac gggggttaat attcctgagt ggactgccgt tcttttcctc tccaatacca
     3721 atagtgctat gaattatctt caagctgcat ttcgagctca aactccattt tctcatgaaa
     3781 aacttggaat gaagaagaat tgttatatct ttgattttgc tccagatcgt gcacttactg
     3841 tcatggctga aagcgcacaa attaactcag gcgtaggtaa gaaaaataca ttacaacaaa
     3901 aagaagctat gacacaatta ctgaatttca tgcctatttt gggacaaaca gaacatggta
     3961 tgaaagtgtt taatgttgat agaatgctta ctcaactcaa aaaagtttat gcggaaaaag
     4021 ctgttcgttc aggatttgaa gacgattcac tttacaatga tgaattactt acacttgatg
     4081 aagcagattt gaatgatttt aataacttga aagaaattgt tggaaaaact aacctatcag
     4141 gattaccaaa gaaagttgaa ataaacgtca atggattaac ggatgaagag tatgaaaaag
     4201 gagaaaaagc tcagaaaaag aaacctcgag aacgcactac agaagaaaaa gaaattattg
     4261 aaaaagtcaa acaagccaaa aaacaacgca aaacaatgat ttcaattttg cgtggaattt
     4321 ccatacgaat tccgatgatg atttatggta tgcctattga ggttgataag gaaatgggaa
     4381 tcgatgaatt tgtcaatcac gttgatagca tttcatggga agagtttatg ccaaaaggca
     4441 ttaaaaagtc tgattttaag cggttcgcaa agtactatga tccagaggtt tttgtcgagg
     4501 caggaagaat tatccgacaa agggctcagt cttatgatgg tttagagtac acagaaagag
     4561 cagagaaaat cgcagaactt tttggaacat ttaaaaaccc agataaagaa actgttttga
     4621 ccccatggag agttgttaat ctacagcttt ctaaaacaat tggcggatta tgttattttg
     4681 atgaaaactt tgaaaacacc acattgaacg gacaagattc gattacttgg gttgataccg
     4741 atatcacaaa agaagtattt aagtccgata ctaaaattct tgaaatcaat tctaaaacag
     4801 gtctatatcc tctctatgta gcaagttcat tgttttatca aaaccgaaat aagttaaacg
     4861 atgaccgagc aggaagattc agcaagattg atgaagatga aataatacaa gaagtattga
     4921 aagaaaatat ttacgtcatt gctaaaacac ccatggcaaa aactatcaca cagcgaacat
     4981 tagcaggtta taataattgg gcaacgaata ttttatatgt ggaagatatt aatcaaaagc
     5041 tcaaaacaaa tatggataaa accattgagg aaatacaaaa aggactgaat gtaatgaaat
     5101 ttgatgtagt tataggaaat cccccatacc aagaaaaaaa tataggaaat agtacacaag
     5161 cacctccaat ttacaataag ttcatggatc tttcttatag cattgcagat aaagcggttc
     5221 ttattactcc tgctagattc ttatttaatg caggtgcaac tagtaaagca tggaatgaaa
     5281 aaatgttaaa tgataaacat ttaaaagttt tattctatga acgagatgca gctaaagtat
     5341 tttctaatac aacgattacg ggtggtgtag ccataactta tagagattct aaaaaagatt
     5401 ttggtgcaat aaaaatattt actaattacg aagatctaaa tttaatagta aagaaagtag
     5461 aaaataaaaa ccctgtatca tttagtacaa ttttttatgg gcaaaatgca tatcaatttt
     5521 tatcagtatt tcatgaagag aatccacaag ctatacaaaa aatgagtaaa ggacatgaaa
     5581 aagatataat aacaaatgtg tttgatactt taaattttgc cttttataat gaaatacctg
     5641 acgatgataa atatattaag atttatggtc gtcaaaataa caagagagta agcaaatata
     5701 ttaaagcaaa atatataaaa aaccatgaaa gcatagacaa atggaaggtc tttatcccca
     5761 aagcaaatgg agcttccggg atgataaccg attctggttt atctagaatt ataagtacac
     5821 cctttatagg agaacctttt tcagggcaca cgcagaccta tatttctgtg ggaaaatttg
     5881 attcagaaga agaagcagaa aattgcttaa aatacatcaa gacaaaattt gctcgcttat
     5941 tattaggagt tttaaaagta actcaggata atcctggacc aaaatggaaa tatgtacctc
     6001 ttcaagactt tacaaaccat tcagatattg actggacaaa atctatatct gatatagata
     6061 accaactata caaaaaatat gatctagatc ttcaagaaat taaatttatt gaggataaaa
     6121 ttgaaaatat ggattaaacg gagattttat attataaaac tagttattta aaccctaagt
     6181 caacataatc tcgtttaaac gtagtaccgc ctggatcggt tgatccaggc ggtttcttta
     6241 tctaactcta ttgctgcttt taaaaatggt aaaaataggg gctataatta gataataacc
     6301 cctataaaaa actattaaaa cagccccttt aatctgacag ttagattaaa ggggctgttt
     6361 atttatttta atgatataat aaccctaaaa ggagtaggct aatgaataga ttttttaaac
     6421 ccattaaata tttagggatc ttgacggttg ttattgttat ttcagctgta ataacttata
     6481 ttatcgatcc taatttatca gatacattaa attctgtatc aagcaatgtc ccaaaatccg
     6541 tatcacaaaa atcaggatta caattagtag ttgcgtatat ttttaataat gggtttaaag
     6601 tcccgatagg tatgttaatt ttatcaatta tacctatccg atttttatat tggattcagc
     6661 cactttttac agtcatacta cctggaatat tatttggtat agtttttaga tattcgttgg
     6721 caaaaggatt cgttatttta atctcctcgc tccctcatat gttatttgag gtcttcgcat
     6781 tttgtgtatg gatggtagct ttggatcaat ttaataaatg gattagatac aaaatatcta
     6841 ggaaaaaaca aacaaatact aagttatttt atgaattcaa gttaatatta atctcatatg
     6901 ttaagtacgt tatcccatta attgttatcg cagcttttac tgaaacttat gttgcggatt
     6961 ggattagtca tatattaagt taaaaaagga gtaataatat ggttcaacaa atcgtcctcc
     7021 ccatcaaaga ttcaaatatc ttaaaaatgg tgcaggatac actacttgat agttttcgtg
     7081 cagggcgtcg caattacacc atctttcaag tggggaaagc cactctctta agggtaagtg
     7141 atgtcatgaa attaaagaaa acagatgtat tcaatcctga tggaacagtc aaacagactg
     7201 ctttcattca tgatcaaaag acaggtaaag cgaatacttt gtatttaaaa cctgtccaac
     7261 aagatttgtt aatatatcat gattggctgg tccaacaaaa tctcaattca gagtggttat
     7321 ttccctcaac ctctcgctct gatcgtcata taacggagaa acagttttat aagatcatga
     7381 cacgagttgg tgagcttcta ggaattaact atctaggaac gcataccatg cgtaaaacag
     7441 gtgcttatcg ggtctatacg cagtcaaatt ataatattgg gttagtcatg cacttattga
     7501 atcactcaag tgaggcaatg accttaacgt atcttggatt agatcaagcc agtcgagaaa
     7561 cgatgttaga ccaaattgat tttgggtaat gaatgtttag aacttaatag tcgtccaaaa
     7621 acgactactt tttttgttga atcgtacgag gacgattcga tgcttttgac ttgtaagaac
     7681 aggattgaat gaagccatac tgttcgcata tttttagtgg atgaacaaac aaaatacgag
     7741 agattttttg ttcgtttatc catgatttta gaaaaaagag ggacgatttc ggaagaagaa
     7801 aatcgtctct tttttttttc ttctttttgt atgacaaaaa gaaagatctt ttgcccattt
     7861 tatttttata aaatgggtag gtggcgtttg cgtaaagcaa atcgacacaa tccaaagggg
     7921 ataaaagggg aaagtgaaac ttccaccttt tcaagccaca ttgtaataca agaacgaaaa
     7981 gtattactac tcatgattgg ttgaatggag gcaaatgaaa taaaattcaa atacatgagc
     8041 gaagcgaatt ttaataaaca cgcttgacgg aaaaagagta cagatcactt acgtgaaact
     8101 gtactctttt ttagcacaaa cctatcaatt tttcgtaaga aaaatttggt gggtataagt
     8161 gcgcccttag taaaaatcgc taagggaata tttttcaaat taattttgta gacacgcacc
     8221 ccttgttttt aagggcgcac ttatacccct aaatttcatt tagtggtaat tgtgctataa
     8281 aacttaattt acatttcgtt ttatcgtcaa gcgtgtttgt taaaactcac gttgttcgtg
     8341 tgatactgtc ggcgtatttt tttattgctc gtaaatagtc atgataggct tctagttctt
     8401 caagagataa tttaaataaa ttaatttgat tcatacttga taattgattt agcaaatttg
     8461 tttgtaggtt cgtagaatcg cttttatcga gttcattatt taaaacatag ttcaacattt
     8521 ttgagtgaat cgctgattga aattcattga ttatctcaat ttcaaatgtt tttctgtaat
     8581 ctaatgtcat gttgtttgct cctttctggt ttcgctttgc tcaactaaac atttactttt
     8641 aattaaacag acttcgctaa tttaatcaaa aataaacgtg cgaataaaaa gcaaatcatt
     8701 tttaaagctc ataaaatgcg ttctaaggcg ttttatgagc tttaggtgta atttatgctc
     8761 tttactttta aaaagcctgt atcagcttga aattagtgtt taaattgatt ttaacctaaa
     8821 tccagggtgc ttacttcttt ttgggtgcgc tagtttattc aaaaggaatc ctgtaaataa
     8881 aaaagtaaca tactccacaa agctctattt ttagttttaa gggggttttt gttctaagtc
     8941 tgcttttggg ttgttagggc ttgccctgac cgtctgtaag acgcttgatt gcgtgatatg
     9001 agtatttagc tagtcaaaca gttaaaacag cttatatgag caattagagg gaatccaata
     9061 aattcctaaa agtggttttg atcttttctt ttagcgagtg aacgaagtga acgctgcaag
     9121 taaaatgtga gcgtggtttt cgctcactcc ttttttgatg actttgacct ttatttttac
     9181 atttttgaaa aaaataaaaa aaaggagaag cctatattac atttatctta tatattttat
     9241 tcttttgttt cttttgcgtg gaaaaaaagt cagtggtagc aaggaaggac agatttttat
     9301 agcaacaaaa aacgatgtat atagcaacaa aaaacgatgt atatagcaac aaaaaacgat
     9361 gtatatagca acaagaactt gattaagttg ctatatattt gtataatgaa agcatagaga
     9421 aaattgacgt ctaaatcact tctctatgct taatcacaaa acactcacaa aggagtattt
     9481 ctatgtctat tatatcagaa aatcaaaagc aggtgtatac cttgaatgaa atcgaaaaac
     9541 gtaaagtggt cgagcataat agcttgatca cttcaatcgc taaaatgcaa aagaccgctt
     9601 taaaaatgtt tgaattagcc gtgtcttgca ttgatacaga aaatccgccc aaagataata
     9661 taatctatct ttcaaaaaaa gaattatttt ctttttttga tgtatcttca gcaagtaaac
     9721 atactcgttt taaagaagcg attgagtata tgcagaaaca agcttttttt caaattaaag
     9781 aagaaaaaaa taaagggtat gaaatgacaa gtatcgtccc aataccaact gttaaatgga
     9841 acagctacaa tgatgatgta atgatacaat ttaatcaatt tattatgcct tatctgattg
     9901 atttaaaggc agaatttaca cagtataaaa tctctgaatt gaaagaactt aatagcaagt
     9961 acagtcttat cttatatcgt tggttagcta tgaattatag ccagtacgag cattatagtg
    10021 ttaaaggtgg acggagagcc gagcaagtgg aagcctaccg caacccctca atcacagtga
    10081 aagagttgcg agaaatcact gatacgatta atgaatatac acgttttcaa agttttgaaa
    10141 accgtgtatt aaaaaatgcc attgaagaaa tcaacgccca tacatccttt aacgtgtctt
    10201 atgagaaagt caaaaaagga cgttcgattg attctatt
//
