LOCUS       NZ_CP071928             9318 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain VRE3355 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP071928
VERSION     NZ_CP071928.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15566637
            Assembly: GCF_017584065.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Genomic surveillance of antimicrobial resistant bacterial
            colonisation and infection in intensive care patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: Enterococcus faecium VRE3355
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017584065.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:55:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,883
            CDSs (with protein)               :: 2,883
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 63 of 134
            Pseudo Genes (incomplete)         :: 73 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 21 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071928.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE3355"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed3"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(join(9300..9318,1..119))
                     /locus_tag="J5F47_RS00150"
                     /old_locus_tag="J5F47_00155"
     CDS             complement(join(9300..9318,1..119))
                     /locus_tag="J5F47_RS00150"
                     /old_locus_tag="J5F47_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(233..853)
                     /locus_tag="J5F47_RS00155"
                     /old_locus_tag="J5F47_00160"
     CDS             complement(233..853)
                     /locus_tag="J5F47_RS00155"
                     /old_locus_tag="J5F47_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(850..2976)
                     /locus_tag="J5F47_RS00160"
                     /old_locus_tag="J5F47_00165"
     CDS             complement(850..2976)
                     /locus_tag="J5F47_RS00160"
                     /old_locus_tag="J5F47_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(3030..3404)
                     /locus_tag="J5F47_RS00165"
                     /old_locus_tag="J5F47_00170"
     CDS             complement(3030..3404)
                     /locus_tag="J5F47_RS00165"
                     /old_locus_tag="J5F47_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3537..3998
                     /locus_tag="J5F47_RS00170"
                     /old_locus_tag="J5F47_00175"
     CDS             3537..3998
                     /locus_tag="J5F47_RS00170"
                     /old_locus_tag="J5F47_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4311..4481)
                     /locus_tag="J5F47_RS00175"
                     /old_locus_tag="J5F47_00180"
     CDS             complement(4311..4481)
                     /locus_tag="J5F47_RS00175"
                     /old_locus_tag="J5F47_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020944745.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKQTDK"
                     /name="hypothetical protein"
     gene            complement(4497..5036)
                     /locus_tag="J5F47_RS00180"
                     /old_locus_tag="J5F47_00185"
     CDS             complement(4497..5036)
                     /locus_tag="J5F47_RS00180"
                     /old_locus_tag="J5F47_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(5064..5801)
                     /locus_tag="J5F47_RS00185"
                     /old_locus_tag="J5F47_00190"
     CDS             complement(5064..5801)
                     /locus_tag="J5F47_RS00185"
                     /old_locus_tag="J5F47_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            6446..6532
                     /locus_tag="J5F47_RS00190"
                     /old_locus_tag="J5F47_00195"
     CDS             6446..6532
                     /locus_tag="J5F47_RS00190"
                     /old_locus_tag="J5F47_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6653..7342)
                     /locus_tag="J5F47_RS00195"
                     /old_locus_tag="J5F47_00200"
     CDS             complement(6653..7342)
                     /locus_tag="J5F47_RS00195"
                     /old_locus_tag="J5F47_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(7344..7622)
                     /locus_tag="J5F47_RS15640"
     CDS             complement(7344..7622)
                     /locus_tag="J5F47_RS15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(7582..8496)
                     /locus_tag="J5F47_RS00200"
                     /old_locus_tag="J5F47_00205"
     CDS             complement(7582..8496)
                     /locus_tag="J5F47_RS00200"
                     /old_locus_tag="J5F47_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8478..8858)
                     /gene="mobC"
                     /locus_tag="J5F47_RS00205"
                     /old_locus_tag="J5F47_00210"
     CDS             complement(8478..8858)
                     /gene="mobC"
                     /locus_tag="J5F47_RS00205"
                     /old_locus_tag="J5F47_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     BGC             1..8404
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5065..5801)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7586..8496)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttcagttg atttagtttc ataagtcttt tgtaattgac ggtttaattt ttttggcaat
       61 tgcaaagaat aaataagtct atcataaaat ttttcatact cttttttcaa taaaatcact
      121 agggtgcctc ctttttatta agtgattatt taaattttgg gataggagag atggaatata
      181 aaataaatac ccgtggtgct agttttaaat cagctagcat cacgggtaaa aattataatt
      241 ctataatatt agttccatag tttagaacat ttaagtcatg tgttactagt acaacggctt
      301 ttttttcttt ttctgttaaa tttttcagaa agttcattat aaaagttgag ttttctttat
      361 ctaaatttcc tgtaggttca tctgcaaata atatatcaaa tggttttaac atatttctaa
      421 ttaatgctat tctttgttgt tctccaccgc ttaagagaaa gattttttct tttaagatat
      481 cttttggcaa agaaaaatct gttaataaat tataaatatg atcattggaa atattactgt
      541 taaacttttt tgctaattcg aaattgtatt gtactgtttg attttcaaga agagcatagt
      601 tttgaaataa atagctaata tgatttcttc gtagacgttg tatatcatgt ttagaaactt
      661 tcttttcaaa atagaataag ttaccggtat attgtttatc tattaggctt aaattattta
      721 acagagtagt ttttccacta ccactttttc ctgttactat aataaaatct ttttctgata
      781 ttgaaaagtt ccaattttgt ttgatctttt ttttattaat tgacagggtg aaattctcaa
      841 ctgataatat cataaaatat tctccttaaa atatttaaaa ctattttttt tataaataat
      901 aatagaagta attagattta ttaaaaagat cataaaaatt ataaaataca agtaagtgtt
      961 ttttgaaaat atcaattgaa aacacatagt agcaaataac tctattgtaa aagggagtaa
     1021 acataaatct aaaacttgac ttattggtga atatccaaat atttttttta aagctatttt
     1081 ttttgaattt atagtaaaac taacaaaaat catttgaaaa ttaattatta taaataatcc
     1141 taatattaaa agatattgta gatatgcatt taatttattt actttttttt gagataataa
     1201 ttcaccagtt tcttgaaaaa cagaataaga aaaatttatg atattttcta ggttaaattc
     1261 ttttatatac ggattaattt gattaaaaaa gttttggttt gaagtatgtt tctgatcaaa
     1321 aaataatcca ccatcactag tcaatatatt tccccaaaat aatgattctg ttatttctgg
     1381 attaataact ctaactatgg gatcagttat tgtatagtta tcttttgcct ttccatataa
     1441 ggtattaaaa ctaaaatact tttggttatt attaacaaat attagtttta tttttaaatt
     1501 tttcaaatta ggatttttat aaggagcttg aggaggagag tcctcaagtc taaactgtat
     1561 gtcttctaaa taattttttt caatcatttt tgcatattct ttatattttt taggtactag
     1621 aatgtaagta gttagtggat cagagtttat taaatttaag tcagtagatt cttcaaaaga
     1681 aataggattg taatatttta gatagtttaa atcaacacat attcctgcac cagcaggttg
     1741 actaacatag tcttcaaaat ttggataaac tttttgtcca ataaaaaatt ctttattatt
     1801 ctcttggata gttgcgtaat tgtatggcgc gataataaaa gttttataat tcttctgtat
     1861 tttataaaca aatttactag cttttttcaa gtagttattt tcttctacgg tgttgtttct
     1921 atttaattga ttagtaatat ttgttttgta taaattagta gttctttccc aatatttaag
     1981 agaattattt tcggcttcat tatgggtatt tattactttt gtagaagcta ttaacagaag
     2041 gatcatgctt aagaatagac agatatttaa tatacaaaga tttaacttaa gtggtcgtga
     2101 attttttaga taatttttta tactagataa atgatagata cttagagtaa ctatgataaa
     2161 taaaaaacta attataaaat gaaaaatact acatagaaga aatgatagga aaaaattttc
     2221 gttactgtaa tatatgctca aaaaaataga taaaatgaaa gttataatca aagaacaaaa
     2281 taagaggttt aactctttaa tagaatcttt tatagtatca aaagagttat agcctaacat
     2341 tttttttaga gtgatttctt tcatttttga aaaagcaaat ataattgtag taattacaat
     2401 aaatatacta agatagcaga taacaagaac aatattagta ttaatttgaa aagtatctga
     2461 ttgattgata ttggaaatag taggtgttcc atattttttc agtacaggaa ttaatttgtt
     2521 tgagtttcct tgtacatata atatttcact taatccaata ttttccacat gagaaaaagg
     2581 ataaatattt atatttaaaa aattatttgg aagttttaat tggtacttag aatttttgtt
     2641 attagtataa tggactttag aatcgtttct tgctaatata ttttttttag aaaatttcca
     2701 gtgtttgtta gcgttagggt ttgtagcaag aatagttagt tggttaacag cagtaaaact
     2761 atattgagaa atattaatat tttcttgtgt agatatttca tacaaagcgt ttatataact
     2821 gttaaagtcc atatcatctt tatattgcat atgaatttca gcggtatttt tatatagcgc
     2881 ttcagaagtc tttataccat ttataacatt agtagaaaaa aatagtattg taatgcagac
     2941 aaaaagtgat aaaacagcgt ttattttttt taacatattt tgaatttcct ttcaaatgta
     3001 aataaaaaag agagtttaaa ctctcttttt taataaacat tccaccaacc agtatttcca
     3061 gtaagtgtgg cattgcatgt tgcataagct cttactccag ctttctgaac tgatcgtact
     3121 tgaccgcctc gtccattttt agctgttgca ctatgtttag ctctagcatg gtagtaattt
     3181 gcataaactt taaatccatt aaccccacag ttgaaatccc cgccgccagc agcatatctc
     3241 aatttactag aagaaaatcc ttcttctgta gcttccattc ctcctccgta aatggcggta
     3301 tggtcttcgg gtacatctag atttaaagct tctgtggcac tagctattcc tccactagtt
     3361 aataagacac ccattaaagc agaaagacat aaaaattttt tcataactta tttctcctat
     3421 cttttttact agaattattt tattttagtg tgttttataa aataaagaac ttttttggcg
     3481 cgttagtatt tttatgataa aattttatgt aaataatcgt ttacatagga gaaaaaatgg
     3541 aggatttagg atctaagcta agagaattaa gagtgaaaaa agggtatact caagaatatg
     3601 tggcaagaaa atttaacgtt acacgtcaaa ctatttcaaa gtgggaaaat aacaaaacaa
     3661 caccagatat agagacacta aaaaaaattt gtgaatttta tagtactaac ataaattcta
     3721 ttttaaatga agataattca aaaaataaaa aaaataattt actggtattg acagctatta
     3781 tttttaatat atttgttact atattaggtt tatttgaatt gattcaaatg aattttttta
     3841 gctttacaca tggaatattt acttggtttg ctctatctgg attaatagta atagatatct
     3901 taaattattt ttatatttac tattcttatt taaaaaatga aaaatttttt tatacaatgt
     3961 ttttccccct agttattttg attattttat taagctaata tgaagtagtt agttatttaa
     4021 ttgcatagtt gacataatgt cgactatgcc aagtagcctc tctaccaagg gatacagggg
     4081 ctcactttgt gatttttcat tggtctaggt caaaagctct ttttaaggct tgattttctg
     4141 cagttgcttc tttccgtttc tttgtgaatt tttaccagtc attttttact ggggtgttat
     4201 ttttcataaa atatgataaa tttttggtag cgcttcataa gcgcgttgct ttttcaagaa
     4261 taaacactca ttatttttta actggctgct cgaaagagca gtctttttta ttatttatcc
     4321 gtttgtttta caaacaattc ttcgttagga ctgttgtaat aatttggatt caatttcaat
     4381 tcaccatgtt tattcccttt attgattact gacaataaca tcggactgaa gcctagaatt
     4441 cctagtaaaa tagaaccgaa tagaagattt tttcttgtca tgaatgttcc ctccagttat
     4501 ttccaaagtt gccaccattt ttgcttcttc ttttttgtgt gtttttctat cgattctgtt
     4561 tcttcagaaa gaatagtagt tgattcatct tctggagttt cttccgtata agctaattgt
     4621 aactgttctt gcagtttttc gatctgttga tttgcctgta gggtgagctg ttgttgctgg
     4681 tcgagtaatt tttgcatatt agaattattt tgctctttga gggaaagttg ttctcgtaaa
     4741 aattcatttt cttcacgcaa cttatctagc aactcgttta caatcacgtt tttgcttgat
     4801 atataaacgt ttttatcgtt ttcttgttca tactcatgca acctatatag taacctttgg
     4861 ttacctaagt tgctagcaag ttgcaatact ccagaacgta atacaacctt ataaccacgc
     4921 tcatttttag ctaataagga cgggtctaat ttatcaacat gtttagagat tgcttgccta
     4981 cttatttcaa ataattcaga taattccttt attgtaaagg tttctttggg ttgcatgata
     5041 attgcctcct aaattgttag gtattatttc tctaaccaat tatctaaggt aattgaaggt
     5101 atattttctt caaaataaat ttgcagagat cctatcttat ttcctttgta ggctttaatt
     5161 tttttgattt caaaagtttt aaaaatagca gggtgagttg aattaacgtt taaaaattct
     5221 tttttcgctt gggcaaaaat ttgtttatca atatctctca tgcgataact ttctggtatt
     5281 gctaatagag ctctaaaatc ttctattttt acaaaccagt gacctttttt agatcttctg
     5341 tctttatgag ccataagttg gcgatataac tctttaacgt agctactctt taagtttgtc
     5401 atattttcta attcgaaacg agtaaaatta gttccgattg aattaagtat atattcaaat
     5461 tcttcagaaa cctttattaa taatgtttca ttcactttat cgattttaaa tttttgaaac
     5521 agatttaatt gttcatatgt tttttcgtct tcataaataa aagacaaagt aatgattttt
     5581 tttgacattt ttattaaatc attagcaaaa cggtcgccac gtgcatttat attgtaatca
     5641 attaattctt ttatttgttt aaatgtaaaa atttcttctt ttgtaccttt tcttttcatt
     5701 ttagaacata ttccccaaaa taaatccata tctatagggg taaattttct taaagggaca
     5761 gaatttaatc cattattata tcgaactata tcgttactca ataaaaatca cctcaataaa
     5821 ttatgttatc atagacataa gaggatgtaa aacacaattt atcccccata gtgtgacaat
     5881 ttatccccca tagtgtgaca atttatcccc catagtgtga caatttatcc cccatagtgt
     5941 gacaatttat cccccactac ctatgtcaat acttactctc ccataaggtt gctaatccct
     6001 aacagttatt attaacagta tatctaacag tatatctaac agtatatctt aagagcacat
     6061 tgaatttccc gcctaaaaaa ctcctacttt ttcctccaaa aaggttcgcg aatttttcta
     6121 aactggaaaa cttgctccct acggtcgcta aaaaggtgtg atcgctatgc tctcatatta
     6181 tactgcgcag tcgcctccgg ctcctttgct aaaaccgttg ttcaaagagg aggtgtgaga
     6241 ctctgaaagc ttgtctaagc cgttctaaga caattcttga ttaatttccc ttaatctagt
     6301 cgagaagcgc cttacaggcg gtcagggcaa gccctaacaa ccctaaaaca agtgatactt
     6361 aaaaacacca cacaaattta attttttaag atataataac ttgtcgccta tccgacagtc
     6421 ttgcgactgg aaggaggtgt atctaatgtt tgaactgttc atttgccccc ttcttgtagg
     6481 agtttgtgtc tctttattca gttattggtt gaataaccga aacaagaagt aaaatcaata
     6541 aaggcgacat caacctgaaa aaaagcccca gagaatccga cctctctggg gctttcgtgt
     6601 atctaatgtt tgatcatttg cacattcagt ttaacatgat tttaaatcaa attcaatttt
     6661 gttgttgctg atctaaccat tccttgacac tttttagata gtttaaggcg gtttcttttt
     6721 ttgtcggatt ttgcatgtta gttgcttgtt gaagtaagat ttcatagcgt tcatatgtga
     6781 cagattgttg tttttctttt ttttcttctt cactggcaaa ccaagggttt gtgtctccaa
     6841 atagatcact acaggcttct tctatctttg tatcttttac atattgcacg tcttttttgg
     6901 ttcgctcata gttgtcttga acctcttttt gctgtttttc tggcaagttt tgataaagac
     6961 gagtgagttt ttccatgttt ttatccgtca aacgtttttc ttgattcaca aaaatatcgt
     7021 tcatttcctc ataacaagtg gtaaactctt ccattttttg tgctaatgct tgctcttttt
     7081 tctttttatc acgcgctgat tcatactttt gagtgacatt tcctaataaa atacttctag
     7141 aaacgcgctt atttccctct aaaacgccga ataagccgct tgaaacgatt atcaaggaaa
     7201 atatacagcc aagaaagaaa aatcgcttaa atcgaatttt agagcctttt aatgaatatc
     7261 ttgtttgaaa aattttatag atgagccaaa tacttcctga aatactaaca aaaatgagta
     7321 gatagggtaa ataaaatgcc attttagcct ccccatacta accaaaatgc gaacagtgtc
     7381 aggactaagc aacataaggg ggcaagaaaa tccaatgatc gttgaataag cacctttttc
     7441 tgttccttat ttcgctggtc taatagctct tgatggagtt ttttaacttg taaatcaatc
     7501 gtagtttgta tcctttcttg cgtataagga acgagaaaag cacgttgttt ctttatttcg
     7561 ttttctatgt cttttaacgt tttagagcga taattcttgt tggtggtctg ttcctctttc
     7621 aacgtgttgt tcatggtttc ttcgagccgg tctaatttcc ggttgttcag gcgtaagagt
     7681 tcgttgttct cttcgtactt cttctgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     7741 ctcgttggtt cgctgttggt tataggttcg ttctcgtctg tccagctcat gtaaaatcgt
     7801 ctccttttcg tagtcctttc ctagtctgtt ggatcgtact tttttctttt tgtggatcaa
     7861 ttcgtaagtg aaatttttcc cgcgattgaa gcaatgaatc cccaatgttt tgagggaatt
     7921 acaaaattgg tcataggttc tggttttttt gtttttcatt gtttgatcga tcgcaatccg
     7981 aatttcgtct ttccaaacag gttcattttt ttcttttaat tttatttcag acatggttcg
     8041 tttttctggt ttctttcctc gctcagtgat tggtaagtta tgtgcaagac aaatctcgtc
     8101 atttaggtca taacatttgt ttagaaatgc ttgaccatga gcttgaaatt tttttccagt
     8161 ctccagatta acggagttaa tgacaatgtg attatgaata tggtctttgt ctgtatgtgt
     8221 gtaaatagct gtttggtgat cgggggctat tttttttgca tactctgccc ctagttgatt
     8281 agctttttca gcggtaattt ctcctggtcg aaaagattga atcaataagt gtgcctgtac
     8341 acggtcgtct tttccataca gcatgcgaat ttgtttcatt tcacttttgg cataatcgac
     8401 gtcacagttt attcctgttt taatggttgc tcgtggttct gcgtaattaa ttgcacgact
     8461 acaagaagtg gttcggctta ccttaattgt tgccatatct tttgtagctc cttttgcatg
     8521 atttttaggt tttcggataa acgttgagcg tcaccagaag caacgtcatg ttggtgtgta
     8581 ttgcaccact tagcaatttg attgatattc cctccagctt tggctaaatc gcgcgcgatt
     8641 gcctgggcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     8701 ttcacgaaag ccggcacact catttgcaaa gcttcagccg attgcttcag ctttgcaaat
     8761 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     8821 gaagaattgc tagccaaatt ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     8881 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaatga gtaaagctca
     8941 ctaaaagggt ttggcaagct ctatatttgc tagccaaata cctacgtatt tagtgcaaat
     9001 aaccgcttgt caaaggggcg caagctcccc cttgaatccc cctcaaaagc cacgctccac
     9061 aatacaaaaa aagctagcaa atgctagcca ctttttgtaa agtttcgctt atttcaccga
     9121 agatgaaatt gttactttgc aacaaaaaac ataaatgctt ttttgtgtaa actactaact
     9181 atttagctaa aactgatagt tttattaaac attcagcaat atttttagcc aattttttat
     9241 cagactttcg gcgagtcatt atgataatat gtccactttt tgttacttct accaaaaatc
     9301 tagcaataac tctcccat
//
