LOCUS       NZ_CP071927             7837 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain VRE3355 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP071927
VERSION     NZ_CP071927.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15566637
            Assembly: GCF_017584065.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Genomic surveillance of antimicrobial resistant bacterial
            colonisation and infection in intensive care patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: Enterococcus faecium VRE3355
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017584065.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:55:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,883
            CDSs (with protein)               :: 2,883
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 63 of 134
            Pseudo Genes (incomplete)         :: 73 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 21 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071927.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE3355"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            join(7622..7837,1..939)
                     /locus_tag="J5F47_RS00110"
                     /old_locus_tag="J5F47_00115"
     CDS             join(7622..7837,1..939)
                     /locus_tag="J5F47_RS00110"
                     /old_locus_tag="J5F47_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            912..1355
                     /locus_tag="J5F47_RS00115"
                     /old_locus_tag="J5F47_00120"
     CDS             912..1355
                     /locus_tag="J5F47_RS00115"
                     /old_locus_tag="J5F47_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            2525..3493
                     /locus_tag="J5F47_RS00120"
                     /old_locus_tag="J5F47_00125"
     CDS             2525..3493
                     /locus_tag="J5F47_RS00120"
                     /old_locus_tag="J5F47_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            3510..4034
                     /locus_tag="J5F47_RS00125"
                     /old_locus_tag="J5F47_00130"
     CDS             3510..4034
                     /locus_tag="J5F47_RS00125"
                     /old_locus_tag="J5F47_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_044383356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            4210..4614
                     /gene="tnpA"
                     /locus_tag="J5F47_RS00130"
                     /old_locus_tag="J5F47_00135"
     CDS             4210..4614
                     /gene="tnpA"
                     /locus_tag="J5F47_RS00130"
                     /old_locus_tag="J5F47_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            4631..5779
                     /gene="tnpB"
                     /locus_tag="J5F47_RS00135"
                     /old_locus_tag="J5F47_00140"
     CDS             4631..5779
                     /gene="tnpB"
                     /locus_tag="J5F47_RS00135"
                     /old_locus_tag="J5F47_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            6301..6582
                     /locus_tag="J5F47_RS00140"
                     /old_locus_tag="J5F47_00145"
     CDS             6301..6582
                     /locus_tag="J5F47_RS00140"
                     /old_locus_tag="J5F47_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            6584..7000
                     /locus_tag="J5F47_RS00145"
                     /old_locus_tag="J5F47_00150"
     CDS             6584..7000
                     /locus_tag="J5F47_RS00145"
                     /old_locus_tag="J5F47_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     Typing          2526..3489
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 actgatatta actatactct tggttcgcca tttaaaatgc tcgatggaga aaatacggca
       61 aatagttcta ttttctttcc agtattttca gacgctgata atacgattaa atatatttat
      121 accgtcaaaa aaaatacaaa tggagaatat tcaggaagta tttcacaatt tttagcaaaa
      181 gaacttcaaa atctagtaga tagtaatata acagatacaa ccttattttt tcaagataat
      241 agcatttatt attccgataa tggacaaatt aaactacttt ggaaatcacc tttaagtaca
      301 gaaaaaaatg gtgtttcttc ctttacagaa aaaaatgatc ttatatccgt aaatataaaa
      361 aataaaatta aaaatttctc taaaaaacaa aattttcctg cggttaaacg tgtaaaaagt
      421 gtctcctccg gagataactt tattatgatt aattggcgta tagcagaaac ccaaacaagt
      481 tttccgtggt gtgctgctta cgtagaagct gctatcttat gtaacaaaac agatgtacgt
      541 gctacttcag ctagagccat tatgcattat acctatccta atttaaacaa cagtcaaatt
      601 gaatcaaaaa gtcttaatca agatcaaata ataaaatatg caaattactg tggtgtttat
      661 cctaaaaaag tagcacgaat actaaacctt tcagaggtcc aaacacaact aagaaaagga
      721 aatgccattt atatgggaac tgctggttat ggaaattata gtaacagccg tcatgcactt
      781 gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt actatgtgtg gaatccttgg
      841 tataactacc caactgtaat aagtgcaaat gcaggaccag ttacaattcc tgtacctgga
      901 ggtggtttta cttgggacaa taccattaca aattggtaaa taaacccatt gctttagtta
      961 tgggtctagt gttagttctt ttcttaacta gttgttcaaa aaaacaagaa atacaaaaga
     1021 ctgatctgat tccttttcta ggaacttggc aaactaaaga tcattcagaa cgtctagtaa
     1081 tcactaattt aaatacttca aaaaatatag aacaatatac catcttagta aaagagaaca
     1141 atcataaaat tcaaatcatt gataaaaatt taaatataaa tgaacaagtt ttcatttaca
     1201 aagcaacaaa taatttacta acactgcgct ctaataaaaa acaattagat tacactttta
     1261 gtttggattc tccaaaaaag ctcgtattta ttttgacaac gaatgatggg acagaaggga
     1321 cagctaaacc aattgaattt tttaaagctg attgatgaat agttgtttga tattacaaaa
     1381 caataatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta gcatttgcta
     1441 gccttttttg tattgtggag cgtgggtttt gagggggatt caagggggag cttgcgcccc
     1501 tttgaaaagc ggttatttgc accaaatacg taggtatttg gctagcaaat atagagcttg
     1561 ccaaacactt tagtgagttt tgtagaaagt acctttaaag gtactttcac attacgaacg
     1621 atacgtccgt aggacgtact gaaagggtgg gatttatgag cgaactaatg caaaatttgg
     1681 ctagcaaaaa aagttaaaaa atatatgcta ataatcgagc gaaatgaaca ctttttcaag
     1741 tgagcgaatc cgccatgaca aagctgaagc agtttatttc agctttgtca tggcgtgtgc
     1801 ctagactttt aaaaaagtcg gcacacgagc aaatatcttt atcaagctct aatttgcgtt
     1861 ttaagacgtt tgaaacgtct aagtatataa atatataaga gtaggtttaa gagtgcttaa
     1921 atcgaatttt agagggcatt cagacaactt tagggttgtt agggcttgcc ctgaccgcct
     1981 gtaaggcgct ttaagaacaa gataagggat ttatactaag actgtcttaa aacgtctcaa
     2041 acgaaggctc atagcttctc aaagagttcc ttatgaacat ttttagcaaa ggagccggaa
     2101 gcgactgcgc agtataatgt gagagcagag cgatcacacc tttttaagcg actgtaggga
     2161 gtaagattcc cagttgagaa aaatgcgcga atctgtttgg agaaaaaaga agtggttttt
     2221 taggcgggaa attttgtccc cctcttttat aatctctttt taaacctctt tttaaacctc
     2281 tttaccgtcg gctatatcct tactctccca agggaatgac gcttttatct acatcgtttt
     2341 gtctgtttat ctacatcgtt ttgtctgttt atctacatcg ttttgtctgt ttatctacat
     2401 cgttttgtct gtttgtacat tgaattagta catcgacgat gttaatataa gatgtagata
     2461 aacaggcatt ttgtatttca taacgtgtat ctcttgtgcg agtaagaaag gagctatttt
     2521 aaatatgtct aatgaaatcg taaagtatca tcatgaatta aatacaattc cgttgcgaaa
     2581 atttacacca gtcgaaatga atttgttctt ttctatcgta tcaagaatgc gtgatgtagg
     2641 tgataaaaaa gtacaattta cattcgagca attaaaagat ttaagcaatt ataaagcaac
     2701 tgcaaatgtt cgattcatag atgatttaga aacaacgtat gacaaattaa tggatttacg
     2761 ttttggtaga agaagtgctg acggtttgca acgtgaacgt tttgttttat ttaatcaatt
     2821 taaaatcgac ggaaaatctg atattccttt tgctgaaata caagttcatg aaaaagcctt
     2881 acccttactt aataatcttg aagaatgggt gcgctattct ctccaacaat tcaatgaatt
     2941 agaaagcagt tattcaaaaa caatgtttcg attactaaaa caatacagaa ccaaaggttt
     3001 cgcttatttt tcaaaagaag actttcatga attacttgat attcctaaaa gttacaacca
     3061 aggtacgatt gatcaacgtg tgttcaatcc aatacgacaa gaactaaccg caatctttaa
     3121 aggtttaaca atcaaaaaga aatacggaaa aggacgggga aagcctgtca ttggctatca
     3181 atttacattt aaacctgaga taaaaaaatc agacgacttt aacaaacatg ccccaacaaa
     3241 agaagaacaa aacaaagcat tagaactagc taaccaacaa gttcaatctg aaaaagcacc
     3301 tacttcatta caagaaaaaa tcaaagaaca aaagcgaaaa ttggaacttt tacaacagtt
     3361 agaaaagtta gaaagagaac aaaataccca aaataaagaa aataatgcac aaaaatcaga
     3421 gataaattcc gatattcctt cagaactaaa aaaagactta ttagacaatc ttcaaaactt
     3481 attcaaagat tgaggggttt ttagatttta tgacagaaaa aacaatcaaa gaacttgctg
     3541 aagaattagg agttagtaaa acagcaatta acaaaaaagt ttctgacgaa aatcgaaaac
     3601 tttggttttc gaaaatcgga aacaagtttg tgattaatga aaccggacaa aaggctataa
     3661 aatcaatgtt ttttaacgaa aacgaaaact caaatcaaca actagttggc gaaaaaacga
     3721 aaaccgaaaa ccacaaaaac gccaaccaag ttcccgaatt agaagtcctt tctctcctag
     3781 aacaacagtt aaatcaaaaa gatatgcaac tacaagaaaa agacgagcaa ataaaacaaa
     3841 tgcaaaaatt actcgaccac caacaacaac tcaccctgca agccaaccaa caaatcgaga
     3901 aactgcagga acaactacag ttgacttata cggaagaatc ttcggaaaat aaatcaacga
     3961 ctctttctga agaaacagaa tcgatagaaa aacaatcaaa ggcggagaaa aaatggtggc
     4021 aattctggca ataaaaccag caatttaaca gaattaggca gaagtcgccc atcctctata
     4081 ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg gtaactcgaa gaccgacaac
     4141 taaggcgtat tgccttttta ttttgtccca tgttacaatc agtctataca agaaatgagt
     4201 tgatataaca tggaattaga tacaaataat cattcagtat ttcttcttta ttaccacctt
     4261 gttttagtaa cgaaatatcg tagacaagtg attgatgatg aaatatctga ttatgccaaa
     4321 actacttttg aaagaatttc agaatcgtat cacattactt tagtggagtg gaatcatgat
     4381 aaagaccacg ttcatattat gttcaaagct caacctaaaa cagaattgac aaaattcatc
     4441 aatgcctata aaagtgccag ctcaagattg ataaaacgtg actttccaag agtcaaacaa
     4501 tttctttgga aagaaatgtt ctggtctaaa agtttttgtc ttctgacaac tggtggcgca
     4561 ccaattgatg tgattaagaa gtatatccaa aatcaaggaa acaatcataa atagaaagta
     4621 ggtgaacttt atggaacaac taaaagcata caaattcaga atctatccaa cagaggaaca
     4681 agaaatcttc tttgccaaat cttttggttg tgtccgtaag gtctacaatc taatgcttga
     4741 tgaccgaaaa aaggcgtatg aagaagttaa aaatgattct tctaaaaaaa tgacttttcc
     4801 gacaccagct aaatacaaga aagaatttcc gtttttgaaa gaaattgata gccttgcttt
     4861 agccaatgcc caacttaatt tagataaagc atacaagaat ttttttcggg ataaatccgt
     4921 tggatttcca cgtttcaaaa gtaagaaaaa tcctgttcag agttatacaa cgaataatca
     4981 aaatggtaca gttgctttga ttgatagtaa attcatcaaa gttcctaaat tgaagtcatt
     5041 agtaagaatt aagcttcata gacaaccaaa agggattatc aaatctgcta caatatcacg
     5101 tcattctagc ggtaaatact atatttcttt gttgtgtaaa gaagaaatta tcgaattacc
     5161 taaaactaat tccacaattg gtattgactt aggtattatg gactttgcga ttctttctga
     5221 tggacaaaaa attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga
     5281 acaacgcaaa ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga
     5341 agctaaaaat tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa
     5401 tcaacgtact gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat
     5461 ctgtattgaa gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat
     5521 ttctgatgtt tcttggtcaa agtttgtgac taaattacaa tacaaggctg actggtatgg
     5581 acgaaaaatc atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg
     5641 acataaagat ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac
     5701 tcatcatgat cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca
     5761 ttctatggga ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg
     5821 gtaaataaga gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa
     5881 gaagctccca cttcaagcgt taagaaccgt caggtttagc taagtggtga gtagttcaca
     5941 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
     6001 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
     6061 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
     6121 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
     6181 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
     6241 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
     6301 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
     6361 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
     6421 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
     6481 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
     6541 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
     6601 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
     6661 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
     6721 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
     6781 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
     6841 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
     6901 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     6961 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     7021 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     7081 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     7141 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     7201 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     7261 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     7321 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     7381 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     7441 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     7501 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     7561 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     7621 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     7681 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     7741 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     7801 cataacttca attcttttaa tggaaaataa caccgaa
//
