LOCUS       NZ_CP071926             6268 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain VRE3355 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP071926
VERSION     NZ_CP071926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15566637
            Assembly: GCF_017584065.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Genomic surveillance of antimicrobial resistant bacterial
            colonisation and infection in intensive care patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: Enterococcus faecium VRE3355
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017584065.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:55:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,883
            CDSs (with protein)               :: 2,883
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 63 of 134
            Pseudo Genes (incomplete)         :: 73 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 21 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071926.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE3355"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            join(5684..6268,1..822)
                     /locus_tag="J5F47_RS00070"
                     /old_locus_tag="J5F47_00075"
     CDS             join(5684..6268,1..822)
                     /locus_tag="J5F47_RS00070"
                     /old_locus_tag="J5F47_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_208284705.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            1272..2240
                     /locus_tag="J5F47_RS00075"
                     /old_locus_tag="J5F47_00080"
     CDS             1272..2240
                     /locus_tag="J5F47_RS00075"
                     /old_locus_tag="J5F47_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_208284706.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2243..2476
                     /locus_tag="J5F47_RS00080"
                     /old_locus_tag="J5F47_00085"
     CDS             2243..2476
                     /locus_tag="J5F47_RS00080"
                     /old_locus_tag="J5F47_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            2469..2876
                     /locus_tag="J5F47_RS00085"
                     /old_locus_tag="J5F47_00090"
     CDS             2469..2876
                     /locus_tag="J5F47_RS00085"
                     /old_locus_tag="J5F47_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_129974410.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQEVNYSPLS"
                     /name="hypothetical protein"
     gene            complement(3013..4161)
                     /gene="tnpB"
                     /locus_tag="J5F47_RS00090"
                     /old_locus_tag="J5F47_00095"
     CDS             complement(3013..4161)
                     /gene="tnpB"
                     /locus_tag="J5F47_RS00090"
                     /old_locus_tag="J5F47_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4178..4582)
                     /gene="tnpA"
                     /locus_tag="J5F47_RS00095"
                     /old_locus_tag="J5F47_00100"
     CDS             complement(4178..4582)
                     /gene="tnpA"
                     /locus_tag="J5F47_RS00095"
                     /old_locus_tag="J5F47_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            4788..5096
                     /locus_tag="J5F47_RS00100"
                     /old_locus_tag="J5F47_00105"
     CDS             4788..5096
                     /locus_tag="J5F47_RS00100"
                     /old_locus_tag="J5F47_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            5100..5462
                     /locus_tag="J5F47_RS00105"
                     /old_locus_tag="J5F47_00110"
     CDS             5100..5462
                     /locus_tag="J5F47_RS00105"
                     /old_locus_tag="J5F47_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     Typing          1356..2230
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.032"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5..819
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.853"
                     /coverage="99.0"
                     /evalue="8.64e-156"
     Typing          5592..6268
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.115"
                     /coverage="100.0"
                     /evalue="5.34e-146"
ORIGIN
        1 cacgctgata aagataatgg tcgagtgctt ttcaataatt ttagaaatca agaagttgac
       61 tctatagaac gattaatgaa tgattacggt tggaaaaggg atattgtcgg taccaacaaa
      121 attaaagata ttcacgaata caaagaaatc atgagtgaaa tttctgaatt gcgccaaacg
      181 gctgtaaaag agcgtgagag cgtttctgat gagttgaggt atatttatag gcaaagagaa
      241 gaaaacgagc gtgataaggc tctgatggct tctgagagag cgaagattga agaagagaag
      301 aaaaatatgc tgaatgtaaa aaaagagatg gcggtcattt tccaaaaaga tatggttcct
      361 ttagaaagtt tcaagaggct tcctgatggt tcgtatcatt tatctgggaa tgattttgtt
      421 gatttgtatc aacgagctac gagagcaaca aatgccaaga gaaatgctga taacatgagt
      481 gagcaatatt tacagatgct tgagcaaaat acagaattaa atgagtatgt agctacattg
      541 gaagaagagc gtcatttgtc tggtagaaaa caattagcag atttacaagt ggaaaataaa
      601 aaactgaaag aagaaaatcg ctctttacgc cagcgtttag agcgattaaa aaacgcattt
      661 caaaaatcga tcactcgttt gtctatccgt tttggtcttg cagaaaagga tatagggctt
      721 tctgaggaat tagaagtatt agagcaggag aaaaccttat tttatcgtca aaacgtctca
      781 cagcataaat tagagcgttc tgaagatgag atcgagtggt agttacgatg tgcagtcaac
      841 ggtaccggag gcggtttgtg ccgaggataa ggagttgacg gctaaaccac tagcctttta
      901 agctgacatt tgctaaaaaa tggccagtcc attttttggc caacctagcg ttagcgaagt
      961 ttgggggcgc ccttttcaaa gggtgcggtt ttatgttttt ggatatttgc atagctaaag
     1021 agaagtgcta ttattgagtt atcaaataaa taaactgaaa aaagagcaaa aaaataaagt
     1081 cgagcaacgc taccaacgtt ctcgaccttt acggaaacta atcgcactag ctccgtaact
     1141 tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt ttggtaatga
     1201 atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt ttctttgagg
     1261 aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga gtgggaaaaa ttaacagccc
     1321 tatctaacag gcggttaaat gtacaaaata tgtacacccc cttaaaaatg cagtggtcaa
     1381 ttgaccgaat tactatcgtt ggaaaactga aggaaaatat ttattatcac actcaaaatg
     1441 atgtgttgat tttagatttt gagcaattaa tgcgggtgaa tgaaggcaat ggctatttaa
     1501 aagcggttgg gaataacggc tggcaattat tagaccaata cgaagaaaat attgcttata
     1561 ttgagatttt gaaatggcaa gatggcaaag ggcggattga ttttaacccg aataagattg
     1621 gtcatttttt agctagttca atgaaaaatt tcattcatga tttatttcta gaaccacatt
     1681 tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt attacacaat
     1741 atcgagtggt tgatcctgtt tctttcaagc caatttatgg gcggtctggg aagctggaaa
     1801 cagcttattg gggcagtcgt gccagcgaac gccagattcg gctatacaac aaaaaattgg
     1861 aacaggaaac caagcgtaaa attgtgccac cagaaataga aacttggtgg cgactggaat
     1921 tgcagttaag gcgtgggaaa gcaacagatt ggcatgcaat ggttcatgaa agtttaaata
     1981 gttttgctag ccctcatttt ctaccacttg atgtaaaaac aaatgataag gtgatgattt
     2041 ttggactgat tgctaaccct gaaatgtggg ggtttttaga gcgtagaatg aaatacaaat
     2101 tgagagatat tttgaaaaga gaaagtcaaa atgatgagtt gacgaatcat ttacgagaaa
     2161 cattttcgga atcagcagat gttttgaaaa aagaattaga cacttggctt caagggttag
     2221 atgtaacgga ggaggaataa gaatggcaga aaaacaaaag cattttggta agaaaaattg
     2281 gttggtgaat ttaacaggtg ataacgttgt cgttgttttt ttggatagca aaaaaatgtc
     2341 cggtaagtta cttgatgtaa cgcagtatga gttgattttg aaaaataata aaattgattc
     2401 cccaatcatt atccctaagc atgctgttaa atatgtttat ggggtagctg aaagtaacgg
     2461 agaagataat gactgatttg ccagaaaaaa tgaccataaa aaaccttgct gatgagttgg
     2521 gtatatctaa aaatacagtg cgcaatcaac tggtcaatgc aggttatgac cttgctcggt
     2581 ataaagataa tggggttatc atgttagaca aggaattgat tggtgttgtg cggtcaattt
     2641 atcggtcaaa aggtggtcaa ttgactggtg atttaccggt caatgatgca ggcaatgatt
     2701 tcccgttaga aaaagaaaat gaatttttgc gtgaacaatt agcggaaaaa gataggcata
     2761 tttctgaatt aacggaaatg attcgtgacc aacaaaaatt gactttgctt cagcaacaaa
     2821 aaaacgatca gttgttacta gaaaatcaag aagtgaacta ctcaccactt agctaaacct
     2881 gacggttctt aacgcttgaa gtgggagctt cttgggaata gagcatactt gatagcgtat
     2941 ttctttacta acagcggtgt ctcttattta ccaagctatc cccgtagttc ctacggttct
     3001 tgatgattgg atctaagcca atcccataga atgtagtctt agaccttcgg tcagaatatt
     3061 gatactagca ttgatatcac gatcatgatg agtatgacaa acaggacaag tccattctcg
     3121 aatttcaaga gatttcttgc catctttatg tccgcactct gaacaaatct gactagacgg
     3181 aaaccattta tctactttga tgatttttcg tccataccag tcagccttgt attgtaattt
     3241 agtcacaaac tttgaccaag aaacatcaga aatactttta gctaatttat gatttcgcaa
     3301 catacctttt gtgtttaagt cttcaataca gataatatcg tgatttttga tcatttctgt
     3361 actcaactta ttcagaaaat cagtacgttg attcattact ttttcatgca atctagctac
     3421 tttgcgtttt tgtttttgat aatttttagc ttcaaataga ttggtacctt tatttttagc
     3481 caataaagca cgcctagaca atttgcgttg ttcacgcttt agtttctttt ccattttgga
     3541 cgtgaattta ttattatcaa ttttttgtcc atcagaaaga atcgcaaagt ccataatacc
     3601 taagtcaata ccaattgtgg aattagtttt aggtaattcg ataatttctt ctttacacaa
     3661 caaagaaata tagtatttac cgctagaatg acgtgatatt gtagcagatt tgataatccc
     3721 ttttggttgt ctatgaagct taattcttac taatgacttc aatttaggaa ctttgatgaa
     3781 tttactatca atcaaagcaa ctgtaccatt ttgattattc gttgtataac tctgaacagg
     3841 atttttctta cttttgaaac gtggaaatcc aacggattta tcccgaaaaa aattcttgta
     3901 tgctttatct aaattaagtt gggcattggc taaagcaagg ctatcaattt ctttcaaaaa
     3961 cggaaattct ttcttgtatt tagctggtgt cggaaaagtc atttttttag aagaatcatt
     4021 tttaacttct tcatacgcct tttttcggtc atcaagcatt agattgtaga ccttacggac
     4081 acaaccaaaa gatttggcaa agaagatttc ttgttcctct gttggataga ttctgaattt
     4141 gtatgctttt agttgttcca taaagttcac ctactttcta tttatgattg tttccttgat
     4201 tttggatata cttcttaatc acatcaattg gtgcgccacc agttgtcaga agacaaaaac
     4261 ttttagacca gaacatttct ttccaaagaa attgtttgac tcttggaaag tcacgtttta
     4321 tcaatcttga gctggcactt ttataggcat tgatgaattt tgtcaattct gttttaggtt
     4381 gagctttgaa cataatatga acgtggtctt tatcatgatt ccactccact aaagtaatgt
     4441 gatacgattc tgaaattctt tcaaaagtag ttttggcata atcagatatt tcatcatcaa
     4501 tcacttgtct acgatatttc gttactaaaa caaggtggta ataaagaaga aatactgaat
     4561 gattatttgt atctaattcc atgttatatc aactcatttc ttgtatagac tgattgtaac
     4621 atgggacaaa ataaaaaggc aatacgcctt agttgtcggt cttcgagtta ccaacggtaa
     4681 ccctcatacc gaacggcatt catcacccac ctatagagga tgggcgactt ctgcctaatt
     4741 ctgttaaaaa atcgaaaatg gtggcaattc tggaagtgag gtgtgacatg gataaaattg
     4801 atttgagaga agaaacagtc gatagaattg aattgatgta tgaacggtta gctgattatt
     4861 tgaatttgcc ggatgaagat aattatagtg aaatgcttga acgaatgatg gatgttctca
     4921 actcggttat caatgctgat ctagcaaaat cttcagtttt gactaatgaa tttgttcata
     4981 agcatttagc gcaaacaact gatgaatcag aaaaagaatt ttggttgaaa ttaatgagtc
     5041 atgttatgca gttaaaccaa atttccgtta ttaattccaa agaaaaggag atgtagttta
     5101 tgggaatgac tttagcagat attaaagcgc aagaagaaaa attaaaaaaa gaacaagcga
     5161 aacttcgcca acagaaaaaa cgcttgattg agaaagaaaa tgctgcaatg ggcaaggctc
     5221 ttcgcaagcg ttttgggttt gaaacttttg ctgattttga gcggtggcta tcgtcgattt
     5281 ctgacgatca gcaacacgaa aaatcagtgg ataatgagtg gcttgctgat gaaaaatcag
     5341 cgccaagcga acgagattat cccgatgaat ctgacggctt attcggagtg ataccgaata
     5401 agaaaagtca gatgagtgag agtaaattta cgtcaatatt taatgacgtg aatttacgat
     5461 aagcgattat tttgcgtgaa gcaaaataaa gcgtaaaagg agttgagagc gtgacacgct
     5521 ttcggctcct tttttcgttg tcacttgtga cagcgaatac cctcggagag catttggaat
     5581 tgtgacagcc atgtcacaat taggaaaatg ttactctttg acaaagagta acagaaaggt
     5641 ttacactctt tttattagcg acagcgcgaa ggagggtttt aacttggggt ttagtatttc
     5701 atttaaaaaa ggaacgaaga atacaagtat ttctcataat aatcgtgagt taactgatag
     5761 ccagaaaacc aatgattggc ataagcatat tgacttcacg aaatcggatg aaaatattta
     5821 tttagagcag acgaatctag cagataaata tgaggaattg tttggcgaag tggtagaaaa
     5881 atataatgcg aaacaaaaaa gagccgatag aaaaatcgag gactatttag cgaaagtgcg
     5941 caaggataaa aagttggaac ctcaacgaga atttattgtg caaataggca ttttagatga
     6001 tttccagaca aatcgagcag atggttcttc tactggcata tcgaaacaac aagctgaaca
     6061 aaatcgagtg attgccaata aaattttggt tcagtattac aaggagtttc aagagagaaa
     6121 ccctaatttg gctgtttata atgctgttat tcataatgac gaaataagtc cgcacctgca
     6181 cttgaatatt gtgccggttg ctgaaggtta taaacggggt gttcaaaagc agccgagctt
     6241 taataaagca ctggttcaac aagggata
//
