LOCUS       NZ_CP071823            10077 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain 90CM2 plasmid
            p90CM2-10k, complete sequence.
ACCESSION   NZ_CP071823
VERSION     NZ_CP071823.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16453377
            Assembly: GCF_018604765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Stosic,M., Leangapichart,T. and Sunde,M.
  TITLE     Co-occurrence of mcr-2 and mcr-3 in Klebsiella pneumoniae in human
            from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Leangapichart,T., Stosic,M. and Sunde,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2021) Animal health welfare and food safety,
            Norwegian Veterinary Institute, Pb. 750 Sentrum, Oslo 0106, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-JUN-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,809
            CDSs (total)                      :: 5,684
            Genes (coding)                    :: 5,540
            CDSs (with protein)               :: 5,540
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 61 of 144
            Pseudo Genes (incomplete)         :: 82 of 144
            Pseudo Genes (internal stop)      :: 33 of 144
            Pseudo Genes (multiple problems)  :: 23 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071823.1.
            Bacteria and source DNA available from Norwegian Veterinary
            Institute, Oslo, Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="90CM2"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p90CM2-10k"
                     /geo_loc_name="Thailand"
                     /lat_lon="16.7005814 N 102.5143558 E"
                     /collection_date="2018-12"
                     /collected_by="Thongpan Leangapichart"
     gene            join(8844..10077,1..452)
                     /locus_tag="IM758_RS28485"
                     /old_locus_tag="IM758_28485"
     CDS             join(8844..10077,1..452)
                     /locus_tag="IM758_RS28485"
                     /old_locus_tag="IM758_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            462..719
                     /locus_tag="IM758_RS28490"
                     /old_locus_tag="IM758_28490"
     CDS             462..719
                     /locus_tag="IM758_RS28490"
                     /old_locus_tag="IM758_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            804..953
                     /locus_tag="IM758_RS28495"
                     /old_locus_tag="IM758_28495"
     CDS             804..953
                     /locus_tag="IM758_RS28495"
                     /old_locus_tag="IM758_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2436..4397
                     /locus_tag="IM758_RS28500"
                     /old_locus_tag="IM758_28500"
     CDS             2436..4397
                     /locus_tag="IM758_RS28500"
                     /old_locus_tag="IM758_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_065799589.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4397..5128
                     /gene="mobC"
                     /locus_tag="IM758_RS28505"
                     /old_locus_tag="IM758_28505"
     CDS             4397..5128
                     /gene="mobC"
                     /locus_tag="IM758_RS28505"
                     /old_locus_tag="IM758_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_032448355.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5135..5665
                     /locus_tag="IM758_RS28510"
                     /old_locus_tag="IM758_28510"
     CDS             5135..5665
                     /locus_tag="IM758_RS28510"
                     /old_locus_tag="IM758_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5693..5872)
                     /locus_tag="IM758_RS28515"
                     /old_locus_tag="IM758_28515"
     CDS             complement(5693..5872)
                     /locus_tag="IM758_RS28515"
                     /old_locus_tag="IM758_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5898..6326)
                     /locus_tag="IM758_RS28520"
                     /old_locus_tag="IM758_28520"
     CDS             complement(5898..6326)
                     /locus_tag="IM758_RS28520"
                     /old_locus_tag="IM758_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6490..6804)
                     /locus_tag="IM758_RS28525"
                     /old_locus_tag="IM758_28525"
     CDS             complement(6490..6804)
                     /locus_tag="IM758_RS28525"
                     /old_locus_tag="IM758_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_215116503.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPQEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6792..7070)
                     /locus_tag="IM758_RS28530"
                     /old_locus_tag="IM758_28530"
     CDS             complement(6792..7070)
                     /locus_tag="IM758_RS28530"
                     /old_locus_tag="IM758_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7412..7630)
                     /locus_tag="IM758_RS28535"
                     /old_locus_tag="IM758_28535"
     CDS             complement(7412..7630)
                     /locus_tag="IM758_RS28535"
                     /old_locus_tag="IM758_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            complement(7627..7998)
                     /gene="tolA"
                     /locus_tag="IM758_RS28540"
                     /old_locus_tag="IM758_28540"
     CDS             complement(7627..7998)
                     /gene="tolA"
                     /locus_tag="IM758_RS28540"
                     /old_locus_tag="IM758_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8272..8517)
                     /locus_tag="IM758_RS28545"
                     /old_locus_tag="IM758_28545"
     CDS             complement(8272..8517)
                     /locus_tag="IM758_RS28545"
                     /old_locus_tag="IM758_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             3844..10077
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1057..1635)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.938"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4398..5125
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.74e-141"
ORIGIN
        1 agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata
       61 aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
      121 cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc
      181 atgattatca tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag
      241 gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata
      301 aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca
      361 gtgatggcga acacctcggg gcatttgatc ccaaaacggg taagcaggtt aaagggccag
      421 atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt
      481 tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg
      541 gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg
      601 gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa
      661 taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac
      721 aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga
      781 gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga
      841 ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg
      901 gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat
      961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa
     1021 aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg
     1081 gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg
     1141 gcgcacactt caagagcaac ctcgtgttta gctaaacaaa tcctctgcga actcccagtt
     1201 accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc aagtgaacag
     1261 ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg
     1321 gagcgaacga cctacaccgg gccgagatac cagtgtgtga gctatgagaa agcgccacac
     1381 ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag
     1441 cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc
     1501 cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct
     1561 gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct
     1621 gccgttttgc ctcacatgct cgttccctgc ttatccacgg aatctgtggg taactttgcg
     1681 agagttcgca gcgcccgccg cagtctcacg gccggcgcgt agcgaccgag tgagcgagga
     1741 ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgtggatgtg acatattgct
     1801 ccgtgttacc gcctttctct tccctgccga taaattattc agataggtga agtatgtcgg
     1861 ttttctgctg aatattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
     1921 tgcacgtcat ccccgctggg gcgtgactga cttcaggggg aaatcccctg cttacgctgg
     1981 ctggacgcca ccgacgcact gtctttcccc ctctttctgg ctctgtccgg ctggacgccg
     2041 gtcacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaatcca
     2101 cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc
     2161 gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc
     2221 gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg
     2281 catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg
     2341 accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcaggggc tgcacaacac
     2401 gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct
     2461 ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc
     2521 gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggcag
     2581 aactctgggc cgggcggatg cgattcgtgg ggctgagtca taaggagcgg cgggagtatg
     2641 ccgatcgcta taagcgcgat aataaccgga ctgcgccatt ttcggtgctg gcctcgctgg
     2701 attcgcccgg gcggatggtc agggcgcggc gacatgtctg gaactacgca tactggctga
     2761 ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct
     2821 ggggagaaac cgtcctgcgt ccggacctct acgcgaacgc agattattac ctgctgcagc
     2881 cggctaacct gttcgggatt gcctggtgtg ccggaggtct tgtcgcaggc gtcctcctgg
     2941 cgcggctgct ggcgttcctg ctgcttgacg ggcattttgc ggccgctgac gaggcggtga
     3001 acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa atgaccgacg
     3061 tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg
     3121 agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc
     3181 tggtcccgcg tgatacctgg cgcaagacca acattcagat cctcggcctg cccgggagcg
     3241 gcaagagcgt gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgcgg
     3301 tggtgtactt cgacccgaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc
     3361 cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg
     3421 atctcgacca gtacgccctg aagaatcttc tggttgccgg atttaacctg agtgagaccg
     3481 gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc
     3541 agtttccgca gggggcaaat attcagcagg tgctggcggc ggcgtacgca ctgccggagg
     3601 ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg
     3661 tccttcagac ggacagcggc atcgacgtgg cggggataat caacggcggc ggctgcctgt
     3721 acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc
     3781 gctgcgcgca aatcatcatt gcgcgggacg agttccggtg gtggccgcac gcgagcatca
     3841 tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc
     3901 gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggccagtgcg
     3961 ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct
     4021 ggttctaccg ggcggccagc caggagtccg ctcagtgggc ggccggacag accggggaga
     4081 tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcatatca
     4141 gcggggacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac
     4201 atctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca
     4261 gcccgctgcg tgttgatcgc cgggaaattc cgctgaaaac cttccctgtg ctggcgaaaa
     4321 cggacccgct ggcggagtac cagtcggagg ccgggcggca gcggccgggt gatgatgatt
     4381 ttgcggggct gtactgatgg cactggagcg atacaacgtc agccacgcga agcggcaggc
     4441 acggaacgcg gagaaaacac ggctgaccct gcgctggctg cgggaagaac tgtgttccac
     4501 ggcggagctg gttgcgcaac ggctggggat tgcggcggta cagccggttt accgcttcct
     4561 ggacagcctg gtggcgaaag ggctgctggt ccgggcgaaa tacccggttg acggccgtca
     4621 ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc tcgttcgacg aggacgagcc
     4681 gctgacggac attattccgt ttcagccgtc gcgggtgtcg gcggcgcagc tgccgcaccg
     4741 gctggccgtg cagtccctgc gcctcgccat ggaggcccgg ggggccaccg gctggcggta
     4801 cctgcaccgt attgccctga aagggatgaa ggtgccggac gcgctggcgg agctggacgg
     4861 cagaacggtg gcgtttgagg tggaacgtac cgtcaagagc cgtcgccggt atcaggaagt
     4921 ggtgtccggg tatctgttca accgtaaggc gaacggcatc gatgaaatct ggtacatctg
     4981 ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg atgaaatcgt
     5041 gaacccgcag accggcgagg cccgcaaaac ggcggagctg gaccgggaga ggctgttcgc
     5101 atgctttaaa tttatgacaa cggagtgagg gattatggcg ctgtacatgg tatttattga
     5161 ggacgtggaa gacaaagacg gcgagcgctg cattatgcca accagcgagc atctggaaga
     5221 catggcggtc atggcctggc agaccggtgc atatgtattc aggtttgaag atcaggccca
     5281 gcatctggca atgatgaagt ttatcggcat gtatcacgaa cggctgaaca gcgatgacaa
     5341 agccacgtat tacttcaccg gcaaaaatcc ggcgtgggaa cgtgagaaac cggccggaat
     5401 gaagctgtgt gattatcttt atcgcaaggt tgagcagttc gccatcgaga acggcgtgca
     5461 ggtactggac gccgggaaat acaccccgga gcagatcggg gcgatgattg acgtcgtgag
     5521 tgagcggtac ggggaaagcg cggtgaacga gaagctgaaa gcggagctga tgcgggaata
     5581 taacagccgc tattttaacg ttccggtgcc agaactgccg gaggataaac ccgcccgccg
     5641 gaggtggccg ttcggcaaaa aataaacgcc agcaccggcc atgccggtgc tgttattctt
     5701 ccccgagctt atcctgcatc acctgccaga gtgtttccgc atcctcgtgc agccgctcgc
     5761 acatgtcggc cggggtatcc atatccagct cgttcagttt ctcgagcaac agcagcgact
     5821 ggtccttaat gaatttagcc atattgaggg cggttttttc ctgcttcgtc atgttctcca
     5881 ttcccttcgg taagttctca gctaaaccag ccggaccaga agcgtttttt cttctgtgtc
     5941 agttctgttg tcaatctgtc cacttcggcc tggagtttcc ggcgctcatc atcgtgctgg
     6001 cggcgttctt cttctcggga tgttttatct tccagcatca gggtgacagc ctgccggaga
     6061 tcggccagtt ccttttgcat tgccagaagc tgttcctgtg ggaaattttt ttgtgtattt
     6121 tcacggtgtg aaatgccggg aatttgtttc tcagggatat tcacatttcc gtatacacgg
     6181 atcagttctg acacatccac gacagggttg tttttcccgt cacgtgacac ggtaaccttt
     6241 ccctgtttta catgattgta tagcgttctt ctggtaatgc ccgcggcttg tgctgcctgt
     6301 gaaaggttga gcaatgtttt cgccactgta tacccctgat tttatcgggt gtgaaagatg
     6361 tgtaatttct cacggtaaat gtatcgctga attttacccg tttttccgtt caggatgtgg
     6421 ttttctgacg cgctgtgctt gtcaaaccgg cgacggccac gcaatgaatt gcgcccccgt
     6481 catcggttat tagaggctgt ctgcccccgg atacccggat tctttctgat gcctgacggt
     6541 gacgatcaca atccggtcca tttcccggtc gtggcggtag agcatcacat agccactgtc
     6601 gccaaacccg atcaccagtt cctgatattc ctgcggcaga aatggcaccg ggcggccaat
     6661 gtctggcaat gttttcagtt gctggatagc ccggacgatc acctcaccgg cttttctggc
     6721 tgccagccga tttttggttt tgagaaagtc ctggaggcgt tgtaaatctc gctgtgccag
     6781 cgcggaaatc gttacctgtg gcatggcgga gcatcctgct cgttctcagt tccccaggta
     6841 cttatccagg cttctgcttc ttccgcagta aggtgcagac ccgtttcctg atagtgctgc
     6901 catgcggctt gtccgtcccg caggtactgg ctgcgcttct cttcccggtc gatgtattcg
     6961 gtgattgcct ccagcattag cgcgtgcgct gagcggtgcc ggtcatcggc cagcgttttc
     7021 agccggtcct taagttcttc atcgagcctg atggatgttg ctgatgacat aaaagcccct
     7081 ttgtagtcat gtgtaataca tatgactact ttaatccgtg gcggcgctat ttgccagaaa
     7141 tacggctttt gctacccatt tttcagcttc gcttccccat ttttcagctt cgcttccact
     7201 aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc gggttaaagg
     7261 acctgttact ggcggcaacc atcatcattg aactttttgg tggtggctgg tcgcggatcc
     7321 tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat tgccgggtgc
     7381 cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa aagtgacagc
     7441 cggtaagcga cacgttgtcc actcgtccat cgtccagaat atcaaacgtg gcggtcacct
     7501 gaccttcgat gcgttttgct gcggcctgta ccgggtaggt tggctgcagt gtggttacgg
     7561 gttttagtgg tatcgcatgt accggagtga ggaacatggc cgccagcact gtcagagtaa
     7621 aagctttcat ggtctgaaat ccagaggggc gtttttgaat atttgccagg tattttcatc
     7681 cggtgccggc ggaattttgg cgtgggtaac ggctgatatt gctgctttac agagtttcgg
     7741 atctcctcct tcagcaatgg cgctgttcag agtgccatca cgttgtatgg atatccgcac
     7801 ggtgcacatt tttcccgcat aggcctctgg ttcaaacatc tgcatttcaa cagcggtctt
     7861 gatggccgca gcgtagctac tgattttaga tgacttatcc agagtataaa ttttcggcgg
     7921 ctgggtggtg gaatgcttac aggccgtcag gagtaagggg ctgcacaatg ccagcaggat
     7981 aaagggtatt aaacgcatcg ggttgatgac ctgtcagtaa aaaatattac atgtatattt
     8041 gccactcttt acggccacgt tcattgacat aaacacggct tatcgtatcg tttttacacc
     8101 aaacggcccg ccatgcagtt gcgtgcatag gcatgcagcg ggttacaatt taccgggcgc
     8161 gtttccggcg gttttccggg tgatttgttg cctgttttat cccgtagccg ccggaaacgc
     8221 tctgagggct tctcgggcgg tgctgataac gggttttatg gtaaataccc ctcaccggcg
     8281 gcgcagcggc tgccaggtga aggtggtttc cgccgcctcg tatttctccc gacgcatttc
     8341 ctgcgcccgc ctggcttcac gttcgtcctg aatggcgcga agcacccttt cccgcacctg
     8401 ctgttttacc gcctttggca gcgttctgtt ccctttcagg cccatggcca gtgcctgagc
     8461 gacaaaggcg tccgctaggc gcttttcttc agcgggagtc agtttcaggt gtgtcatggt
     8521 ggcctccggg cgtctcaggt ttcctgaaca gtttaaccgc gggtcctccc ctcataccag
     8581 ttagctttca gatgtgtcta taccacctga aatgtaagtg tgatgtacat ctcaaataat
     8641 aaaatttgtt gccgtgagaa attttcggga aaagacgcga tctgcttaca ggtggctccg
     8701 ggcgcttttt tgtgcctgaa gatgagaact aatctcatat attgacacct gaaaacggga
     8761 ggagtaaggt aataattgta ctgtgtatat gtacagtatg ttggctttaa tttattgttt
     8821 taaatgtcaa agaggaaaac gatatgagtg gtggagacgg tcgaggtccg ggtaattcag
     8881 gtctgggaca taatggtggt caggccagtg ggaatgtgaa cggtacgtct ggtaaaggtg
     8941 gcccttcatc aggtggtggt acggatccaa acagcgggcc gggctggggt acgacgcata
     9001 cgccgaatgg agatattcat aactataatc cgggggagtt tggtaacggc gggagtaaac
     9061 ccggtggtaa tggtggtaac agcggcaatc atagcggtag ctctggtggc ggacagtctt
     9121 cggccactgc gatggccttc ggtctgcctg ccctggctac tccgggggct gaaggactgg
     9181 ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc tgatgtgctg gctgccctga
     9241 aggggccgtt taagtttggt ctgtggggga ttgcgatcta cggtgtgctg ccttctgaga
     9301 ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac gtcattaccg gccgatgcgg
     9361 tgacggagac tccggcaagt actttaccac tggaccaggc gacggttcgt gtcagacaac
     9421 gggttgtgga tgtggtgaag gatgagcggc agcatattgc ggttgtcgca ggtcggccaa
     9481 tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga
     9541 ttccgggtct cccgtctctg caggtgagcg taccgaaagg tgttccgaca gcgaaagccc
     9601 cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc ggctggtttt acagccggtg
     9661 gtaactcccg tgaggccgtt attcgtttcc cgaaagagac cggacagaag ccggtttatg
     9721 tgtcggtgac agatgttctt accccggcac aggtaaaaca gcgtcaggag gaagaaaagc
     9781 gtcgccagca ggcatgggac gccgctcatc cggaagaggg gctgaaaaga gactatgata
     9841 aagcgaaagc tgagctggat gccgaagata aaaatattgc gaccttaaac agccgcattg
     9901 catcgacaga gaaggcgctc cccggtgcaa gggctgctgt tcaggaagcc gataaaaagg
     9961 taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct catgaatatg
    10021 gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat cagaatg
//
