LOCUS       NZ_CP071716             6675 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Escherichia coli strain EC20017429 plasmid pL5, complete sequence.
ACCESSION   NZ_CP071716
VERSION     NZ_CP071716.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18140782
            Assembly: GCF_023921205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Yousfi,K., Walker,M., Bernaquez,I. and Bekal,S.
  TITLE     First report and genomic analysis of multidrug resistance Shiga
            toxin-producing E. coli producing (O118:H2) harboring ESBL, mph(A)
            and blaLAP-2 in human in Canada
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Bekal,S. and Yousfi,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2021) Bacteriologie, Laboratoire de Sante Publique
            du Quebece, 20045 Ch Ste-Marie, SainteAnne-de-Bellevue, Quebec H9X
            3Y3, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. Galaxy version 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Nanopore Technology
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:54:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,022
            CDSs (total)                      :: 5,893
            Genes (coding)                    :: 5,596
            CDSs (with protein)               :: 5,596
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 121 of 297
            Pseudo Genes (incomplete)         :: 170 of 297
            Pseudo Genes (internal stop)      :: 62 of 297
            Pseudo Genes (multiple problems)  :: 52 of 297
            Pseudo Genes (short protein)      :: 4 of 297
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071716.1.
            Bacteria and source DNA available from Laboratoire de Sante
            publique du Quebec, Canada.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20017429"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pL5"
                     /geo_loc_name="Canada: Quebec"
                     /lat_lon="46.81 N 71.21 W"
                     /collection_date="2017-04-29"
                     /collected_by="LSPQ"
     gene            complement(join(5131..6675,1..549))
                     /gene="cda"
                     /locus_tag="JQM62_RS29975"
                     /old_locus_tag="JQM62_29735"
     CDS             complement(join(5131..6675,1..549))
                     /gene="cda"
                     /locus_tag="JQM62_RS29975"
                     /old_locus_tag="JQM62_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            813..1058
                     /locus_tag="JQM62_RS29980"
                     /old_locus_tag="JQM62_29740"
     CDS             813..1058
                     /locus_tag="JQM62_RS29980"
                     /old_locus_tag="JQM62_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(1548..1781)
                     /locus_tag="JQM62_RS29985"
                     /old_locus_tag="JQM62_29745"
     CDS             complement(1548..1781)
                     /locus_tag="JQM62_RS29985"
                     /old_locus_tag="JQM62_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137424.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVLRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1788..2330)
                     /locus_tag="JQM62_RS29990"
                     /old_locus_tag="JQM62_29750"
     CDS             complement(1788..2330)
                     /locus_tag="JQM62_RS29990"
                     /old_locus_tag="JQM62_29750"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_100029810.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2380..2817)
                     /locus_tag="JQM62_RS29995"
                     /pseudo=""
     CDS             complement(<2380..2817)
                     /locus_tag="JQM62_RS29995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3026..3349)
                     /locus_tag="JQM62_RS30000"
                     /old_locus_tag="JQM62_29760"
     CDS             complement(3026..3349)
                     /locus_tag="JQM62_RS30000"
                     /old_locus_tag="JQM62_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3417..3608
                     /locus_tag="JQM62_RS30005"
                     /old_locus_tag="JQM62_29765"
     CDS             3417..3608
                     /locus_tag="JQM62_RS30005"
                     /old_locus_tag="JQM62_29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3760..3948)
                     /locus_tag="JQM62_RS30010"
                     /old_locus_tag="JQM62_29770"
     CDS             complement(3760..3948)
                     /locus_tag="JQM62_RS30010"
                     /old_locus_tag="JQM62_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(4655..4801)
                     /locus_tag="JQM62_RS30015"
                     /old_locus_tag="JQM62_29775"
     CDS             complement(4655..4801)
                     /locus_tag="JQM62_RS30015"
                     /old_locus_tag="JQM62_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(4871..5134)
                     /locus_tag="JQM62_RS30020"
                     /old_locus_tag="JQM62_29780"
     CDS             complement(4871..5134)
                     /locus_tag="JQM62_RS30020"
                     /old_locus_tag="JQM62_29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3694..3777)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="1.15e-36"
     Typing          complement(4239..4426)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(1442..3036)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3409..3611)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.85e-12"
ORIGIN
        1 agccatatat tgcgtaccgt tctgcataag gtccatcaga cgatatttca ccggtacatt
       61 attacccgct gctggtaact ggctggccgg aatatccgtt acctgttcca gttgctgtgt
      121 aaccataaga cgaataattt tttgctcatt tccggaagca ggctccagac gggcaaaaga
      181 aacagcagcc gggcgagacg gttctggcaa accaccacgt aaaccattct caagaactgt
      241 tcgtgcagcg gaaactttct cgtttcccca agggctatgc acactgacag accatcccga
      301 ccagtctccc tcttcaagtt ccttatccag aataatccgg cgaataattc ccggaaaaag
      361 aacactttgt acaagtgcga taccctgtgg gctgtaatcc ccccatcccg gaggcatgga
      421 tggagcgata cctgatgagc ctcctggttt cacatcacca cccgaaatca gtcctgttga
      481 cggccacacg accatcgagt gcccataatc aataccttct gtcggaccac taccttcgta
      541 atcactcata aaaacacctc tttgactttt aaaacaatgc gttaaaaaca acaaactgta
      601 cataaaacca ctggttttat gtacagtaaa aagttacgtc acctcgtaaa tcctgtcaac
      661 ccactggatt tacgtttttg tgaaaacgct caagaaaaac aaccaatcat tcttaaactc
      721 aatggcaaca ggcatgaaag aaggctgatt agcataagga gagagccaac agttaaactg
      781 ttatgacacc agagaaacac ccggagacca ccatgaaacg ccatagactg accgaagccg
      841 aagagcgccg actggcagaa gccattgccg ctgaagctcg tgccatgggg ctgacgaaaa
      901 aggacaaaat gccgcaggaa acaaagcgac gcctccgggc acggatactt aaagccattc
      961 ttgatgaacg aacggccagc aaaaatcgag aagccacact gactgccgga caggacccgg
     1021 atgacacctt cacctggcaa ccactgcgcc gccgctaagc taagggttat ttaacataac
     1081 cccttattgt acgcaccgct gaaatgcgtt cagcgcgatc acggctgctg acaggtaaaa
     1141 atggcaacaa accacccgaa aagttgccgc gatcgcacct gataaatttt aaccgtatgc
     1201 atagctattc agccatgtga ataacgctgg ttttgcctgc gtaaacctca tgacactgtt
     1261 ttttttccat cttttcagtt gatgacatac gcagacatcg cgggatgagg ctgaggaatg
     1321 agcgcgatct ggcaaagagg caaaacacag caacaaaaac gacacgccag aatcgcgccc
     1381 ggatgcgttt ttaacgcgtt ccggtaccat ctggcaacct cccggaacaa ctcaccgtca
     1441 catccccatt gacgggccac gccatacccg tgcttcccgt tcctgctctt catgccagga
     1501 ccgcgcacgc tcccgttcca ggcgtgcctg cctttcctgt tcatccctta tctgctgttc
     1561 gtgataaata accgactcaa gcggtccacc tgcccggcta atctcagcac ctgctgactc
     1621 aagtcgtcgc actgttccct cagttgcccg ttctcctgtc gtgtcagctc gaacatatgc
     1681 tgcaaatccg tgaaggcgct ctcccagtct ttcagccgct gcgtatagtc ctgctgcaat
     1741 tgctctaagg cgttcagtaa gtgcatttcc agctctgtca tactcactta ctccctgacc
     1801 agtcttactg cgttcttctt ctccaccgtc cagttgtttt cccccttcac cccggacggc
     1861 aacaccagaa atttcccgtt cctgccctcg tgatacgtca caccccatgt tttttcccgg
     1921 agtttcgcca gcgtctcttc ctggtccctg atagccagga tgttcgccgc aatacggctt
     1981 tcctgccact gaatcagccc ccatgacgcc agaaaaatcc cgaccgtgac gcaaagcgcc
     2041 gtcagcgacg ggtacagtat ccgccctttg accagcttcc agagcagctc ttcctgccgc
     2101 cgggcaagtt cgttctctgt ggcgctgaac tgcgcgttca cggcactgtt cagcgtctcc
     2161 agttgttctt tcaccgctgc tgtgtgtgcg ctgatagcgt ctctgatttt ctgcccgttt
     2221 aagttcagtt ccctgtctac agacgcttcg agcttcctga actcgctgtt cagcatgttc
     2281 tctgtagaga cggcacgctc tttcagtttc ttctcgaagt ctgtccccat ttgtaaaaga
     2341 ttgctcatac agcgcccctt tcagcctgag attacgcccg ccctccgggt cggcgatact
     2401 gatactgctc ctggttgtcc tcaccacctc aaaacctgcc gctgtaagcg cctcagtgac
     2461 atcctgacgc gtttttagcg ctccggcatg gtaaagagcc tccagtcccc tcgtaatcgc
     2521 ttctgcggcc tcctgtttcg ctttcggcag attattcggg gtgacaagtg tccgcctgtt
     2581 ctccggtgcg ttcgggtcgt gcagcccgta atggtgattc accagtgtct gccaggcatt
     2641 gattcgcgga cggtccgccc ggtcgtaata gggctggagc cgttttccgc tcgccagctc
     2701 catattcggg atgacaaaat tcagctcaag acgccccttg tcctggtgct ccacccacag
     2761 gatgctgtac tgattttttt caagaccggg catcagtacc cgctcaaagc tctccatcac
     2821 cctttcacgc tctcccggtg gcagtgtctg ctctgcaaaa gacagaaccc ccgaggtgta
     2881 ttttttcgca aacggcgtgg catcgatgag ttcccgcacc tcttcgggag caccccgcag
     2941 aactctcgcc ccttcccggt tacgctcccg gcccagcagg taatcaaccg gaccactgcc
     3001 accgcctttt cccctggcat gaaacttaac tatcatcgcg ttctccctgt ttacggacct
     3061 catccctcag ctcactcagt tcacgtccga tggccatcag tgcagccacc acatgaaccc
     3121 ggtcatgccc cgaccactgt ccactgttta tcttccgggc tatctgattc aggttattgc
     3181 cgaccgaagc gaactggcgc aacagcggcg gtgccagtgt cggaagacct gacgttttcg
     3241 atggcggtgc ccccaggcag accttacgca tccatgacgc aagttgtttt ccctcacaac
     3301 gtgccagcag ccgcgcatgt tcctcatccg tgacccgtat cgtgagcatc ctttcgcgtt
     3361 tcaccggtat cattaaaaac ctccgacaga ctccccacac atggagaaac agaactgtga
     3421 ctaaacagga aaaaaccgcc cttaacatgg cccgcttcat cagaagccag acgctgaccc
     3481 tgctggaaaa actgaatgaa ctggacgccg acgaccaggc tgacatctgc gaagcgcttc
     3541 acgaccacgc tgacgagctt taccgcagct gcctcgcacg cttcggggat gacggtgaaa
     3601 acctctgaca catgcagctc ccggagacgg tcacagcttg cctgtgagcg gatgccggga
     3661 gcagacaagc ccgtcagggc gcgtcagcgg gttttagcgg gtgtcggggc gcagccatga
     3721 cccagtcacg tagcgatagc ggagtgtata ctggcttaat catgtggcat cagtgaggat
     3781 ggtatgaaaa gtgcaccatg ccgggtgtga aatgccgcac agatgcgtaa ggagaaaatg
     3841 ctcgtccagg cgcttttccg cttcctcgct cactgactcg ctacgctcgg tcgttcggct
     3901 gcggcgagcg gtatctgccc actcaaaagc ggtgatagtg ttatccacag aatcagggga
     3961 taacgccgga aagaacatgt gagcaagaaa cgaaaacccc gaaaaaggcc gcgccggagg
     4021 cgctttttcc ataggctccg cccccctgac ggacttcaca aaacagacac tttttcggtg
     4081 catctgtggg agccgtgagg ctcaaccatg aaatccgcca gcggtggcga aacccgacgt
     4141 ggcttaaaga tcatcaccgt ttccggtgaa tcgctccctc ctgcgccccc acgttcccgc
     4201 catatcgatt accggatacc ttttccgcct ttctcccttg cgggacacgt ggcgctttct
     4261 catagctcac gtcttctggt atctcggggc gacttaggca gttcgctccg ggctgggctg
     4321 tatgcaggaa ccccccgttc agcccgactg ctgcaccttc tccggtaact gttctcttgg
     4381 ggccaacccg gaaaagacac gacttaacgc cactggcagc agccattggt aactgattta
     4441 gaggagttgc tcttgaagtt atgaaccagt taaggctaaa ctgaaaagac agttttggtg
     4501 actgtgctcc tccaagccag ttaccaccgc cgattagaag aggtcgaaga cttctgatcg
     4561 gacggaagga cccgccacaa ggcgggtttt ttcgtttaca ggcagcagat tacgcgcaga
     4621 aaaaaaggat ctcaagaaga tcctttaata tttttcactg aaccgccacc ccgattagtt
     4681 tcgaagatga tgatggagca attgttccac cctgaacatc ccttacatag ttagcttggc
     4741 aagcagtcag acacaaaaca gcaagaacga gaatcataga tccccctatc ttttttttca
     4801 tgacatctcc ttgtcatgag tgaatatctg acatttatac ttaaatgcca gatattaaaa
     4861 tcactcatga ttataattta aatttttcca agatagatcg gacctcaacc ttaagtccat
     4921 tatcatcaat ttcataatca gccctatctg catcaggctc aaatcgctca gcagccataa
     4981 acaactcttc cccacaattt aatatattcg gagacaaatc cttaacacca tacaatcttc
     5041 tccgttcaac acaaattcgc tctgaaaact caattgctgt aattttcgaa gctaaaaata
     5101 atttcgccaa atcgatcatt gccatcttgt tcatagttca cccgtctcta aataaattcg
     5161 accattatcc gcatctggat ttattttcca cccagataag aactcaccat ttgacttaat
     5221 tataaccaca ttcatcgtat taggattaaa ataaactttt gaaccttttt ctcttcggta
     5281 tgttcctttc tctactgtat ccttatccga taaatgctcc tcaatagcat ccctgaattt
     5341 agtaagagtt tcacgattct tttttgtatc actaatacca aaatcaccag catgtttata
     5401 ttttttatca agttgtttac gcgaaaatcg ccctgaatcc agaggatcat taagcatcac
     5461 ataaacagct tcaacaccag tcccagcagc atcaggacgc cagtaaataa aatctcgtag
     5521 tccaccaaca tcagaaacag gagtaatcgt cgttactggc atttcgaccg gttttacttc
     5581 cgttcctgta tgcactggtg taaccggaac gggttgatga ttacctgtat ctgttggcac
     5641 cgaaggttgt ggtaccggat taaccagaat cgtccgggaa ggtgcgccta ctactgctgg
     5701 taacgtgatt ttatccagtc ctgttgctgt atcacggaca gcattaagaa caggaaccgc
     5761 agctgggata ctttcattcc ctgttttcag taaatccaga gctaattgcc cgttactgtt
     5821 tatcaattgt ccacgaacag gcagatttac actcgtcgca ccaggttcaa tgaccacgcc
     5881 ctgtccagcc aacatctgag cactcagcga aaacatcgcc tccagatttc gccccggcac
     5941 gatatcactt cctgctccag cttcttctga atgaaaccac agagcaccaa gagaaatccg
     6001 cataagcagg ggactcactt cgggtatggt tactttctcc agagcattac tgaaaaactt
     6061 gcttatgttg cctgcaatat caccagcagc agtccagaca ccagttccgg tcatcgccag
     6121 ttgtacagaa ctggcagtgt tattcagaac aagagctccc tgctcattca ttactgaatt
     6181 agccctttcc atcatttcct gtgtatatac aggaggctgg ttcacacctg ctttactaaa
     6241 taactcggct tttgcttttt cagcttcagc ccgggctacc gcttcttctt tcttgactct
     6301 gaaatcatcc tttgctttat tttcggcttc ctgacgctga ctcagaccat tactgtccag
     6361 aatctctaca gttgaaatat ataacggaga aacaccactg ccatcaggaa aacgaacaat
     6421 gacatcatga ttattactgg acatcgtata accagcctca acataattac ctgtctcagc
     6481 aataactcct tcttcctgaa tttcgggatt agacagaacc ggagtattca tctgacccac
     6541 acttaatacc aggtccggta gatttggtga tacaggggca ctgtaaacat ctttaatatt
     6601 tgttcccgga cgactccggt ccggaactgg aacggcatca actaccggca ctgtcatcgg
     6661 aatacctccg ataat
//
